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Integrating routine blood biomarkers and artificial intelligence for supporting diagnosis of silicosis in engineered stone workers Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-06-29 Daniel Sanchez‐Morillo, Antonio León‐Jiménez, María Guerrero‐Chanivet, Gema Jiménez‐Gómez, Antonio Hidalgo‐Molina, Antonio Campos‐Caro
Engineered stone silicosis (ESS), primarily caused by inhaling respirable crystalline silica, poses a significant occupational health risk globally. ESS has no effective treatment and presents a rapid progression from simple silicosis (SS) to progressive massive fibrosis (PMF), with respiratory failure and death. Despite the use of diagnostic methods like chest x‐rays and high‐resolution computed tomography
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Assessing the impact of long‐term storage on the quality and integrity of biological specimens in a reproductive biobank Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-06-26 Zhao Wang, Changming Zhang, Xin Zhang, Yuehong Bian, Yongzhi Cao
Biobanks hold a pivotal role in facilitating translational and clinical research endeavors. However, the effects of prolonged storage on frozen blood samples analytes are not well defined yet. The aim of this study was to investigate the long‐term stability of the quality of DNA, RNA, and endocrine markers within blood samples amassed from the biobank over the past 11 years. The results show that the
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Propionate‐producing engineered probiotics ameliorated murine ulcerative colitis by restoring anti‐inflammatory macrophage via the GPR43/HDAC1/IL‐10 axis Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-27 Guangbo Kang, Xiaoli Wang, Mengxue Gao, Lina Wang, Zelin Feng, Shuxian Meng, Jiahao Wu, Zhixin Zhu, Xinran Gao, Xiaocang Cao, He Huang
Inflammatory bowel disease (IBD) is a chronic and unspecific inflammatory disorder of the gastrointestinal tract, and current treatment options often fail to maintain long‐term remission. Studies have shown that propionate level is reduced in fecal samples from patients with IBD. Propionate can ameliorate IBD through intestinal epithelial cells and immune regulation, but its effects on the inflammatory
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Enabling next‐generation therapies: A foreword to a special issue on nanotechnology in medicine Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-24 Josué Sznitman
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Statistical process monitoring creates a hemodynamic trajectory map after pediatric cardiac surgery: A case study of the arterial switch operation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-16 Daniel P. Howsmon, Matthew F. Mikulski, Nikhil Kabra, Joyce Northrup, Daniel Stromberg, Charles D. Fraser, Carlos M. Mery, Richard P. Lion
Postoperative critical care management of congenital heart disease patients requires prompt intervention when the patient deviates significantly from clinician‐determined vital sign and hemodynamic goals. Current monitoring systems only allow for static thresholds to be set on individual variables, despite the expectations that these signals change as the patient recovers and that variables interact
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Hydrogels in the clinic: An update Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-16 John R. Clegg, Kolade Adebowale, Zongmin Zhao, Samir Mitragotri
Hydrogels have been used in the clinic since the late 1980s with broad applications in drug delivery, cosmetics, tissue regeneration, among many other areas. The past three decades have witnessed rapid advances in the fields of polymer chemistry, crosslinking approaches, and hydrogel fabrication methods, which have collectively brought many new hydrogel products, either injectable or non‐injectable
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Pilot PET study of vaginally administered bioadhesive nanoparticles in cynomolgus monkeys: Kinetics and safety evaluation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-10 Molly K. Grun, Praveen Honhar, Yazhe Wang, Samantha Rossano, Minsoo Khang, Hee Won Suh, Krista Fowles, Harvey J. Kliman, Alessandra Cavaliere, Richard E. Carson, Bernadette Marquez‐Nostra, W. Mark Saltzman
Long‐lasting vaginal dosage forms could improve the therapeutic efficacy of vaginal microbicides, but achieving long‐term delivery to the vaginal canal has been a significant challenge. To advance understanding of vaginal dosage retention and biodistribution, we describe a method of noninvasive imaging with 89Zr‐labeled bioadhesive nanoparticles (BNPs) in non‐human primates. We additionally examined
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Therapeutic potential and impact of nanoengineered patient‐derived mesenchymal stem cells in a murine resection and recurrence model of human glioblastoma Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-08 Rawan Al‐Kharboosh, Alex Bechtle, Stephany Y. Tzeng, Jiaying Zheng, Sujan Kumar Mondal, David R. Wilson, Carlos Perez‐Vega, Jordan J. Green, Alfredo Quiñones‐Hinojosa
Confounding results of engineered mesenchymal stem cells (MSCs) used as cellular vehicles has plagued technologies whereby success or failure of novel approaches may be dismissed or inaccurately ascribed solely to the biotechnology platform rather than suitability of the human donor. Polymeric materials were screened for non‐viral engineering of MSCs from multiple human donors to deliver bone morphogenic
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Antibody drug conjugates in the clinic Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-04 Edidiong Udofa, Disha Sankholkar, Samir Mitragotri, Zongmin Zhao
Antibody‐drug conjugates (ADCs), chemotherapeutic agents conjugated to an antibody to enhance their targeted delivery to tumors, represent a significant advancement in cancer therapy. ADCs combine the precise targeting capabilities of antibodies and the potent cell‐killing effects of chemotherapy, allowing for enhanced cytotoxicity to tumors while minimizing damage to healthy tissues. Here, we provide
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A stretchable, electroconductive tissue adhesive for the treatment of neural injury Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-03 Jharana Dhal, Mahsa Ghovvati, Avijit Baidya, Ronak Afshari, Curtis L. Cetrulo, Reza Abdi, Nasim Annabi
Successful nerve repair using bioadhesive hydrogels demands minimizing tissue–material interfacial mechanical mismatch to reduce immune responses and scar tissue formation. Furthermore, it is crucial to maintain the bioelectrical stimulation‐mediated cell‐signaling mechanism to overcome communication barriers within injured nerve tissues. Therefore, engineering bioadhesives for neural tissue regeneration
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Hemostats in the clinic Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-05-01 Maithili Joshi, Zongmin Zhao, Samir Mitragotri
Given the prevalence of hematological conditions, surgeries, and trauma incidents, hemostats—therapeutics designed to control and arrest bleeding—are an important tool in patient care. The prophylactic and therapeutic use of hemostats markedly enhances survival rates and improves the overall quality of life of patients suffering from these conditions. Since their inception in the 1960s, hemostats have
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Implantable and transcutaneous photobiomodulation promote neuroregeneration and recovery of lost function after spinal cord injury Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-26 Andrew R. Stevens, Mohammed Hadis, Alice Phillips, Abhinav Thareja, Michael Milward, Antonio Belli, William Palin, David J. Davies, Zubair Ahmed
Spinal cord injury (SCI) is a cause of profound and irreversible damage, with no effective therapy to promote functional recovery. Photobiomodulation (PBM) may provide a viable therapeutic approach using red or near‐infrared light to promote recovery after SCI by mitigating neuroinflammation and preventing neuronal apoptosis. Our current study aimed to optimize PBM dose regimens and develop and validate
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Model acetylcholinesterase‐Fc fusion glycoprotein biotechnology system for the manufacture of an organophosphorus toxicant bioscavenging countermeasure Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-26 Thomas G. Biel, Talia Faison, Alicia M. Matthews, Uriel Ortega‐Rodriguez, Vincent M. Falkowski, Edward Meek, Xin Bush, Matthew Flores, Sarah Johnson, Wells W. Wu, Mari Lehtimaki, Rong‐Fong Shen, Cyrus Agarabi, V. Ashutosh Rao, Janice E. Chambers, Tongzhong Ju
Organophosphate (OP) toxicants remain an active threat to public health and to warfighters in the military. Current countermeasures require near immediate administration following OP exposure and are reported to have controversial efficacies. Acetylcholinesterase (AChE) fused to the human immunoglobulin 1 (IgG1) Fc domain (AChE‐Fc) is a potential bioscavenger for OP toxicants, but a reproducible AChE‐Fc
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Eye of the future: Unlocking the potential utilization of hydrogels in intraocular lenses Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-24 Hao Wu, Jiale Wang, Wenhui Fan, Qi Zhong, Rongyue Xue, Siyu Li, Zongming Song, Ye Tao
Hydrogels are distinguished by their exceptional ability to absorb and retain large volumes of water within their complex three‐dimensional polymer networks, which is advantageous for the development of intraocular lenses (IOLs). Their innate hydrophilicity offers an optimal substrate for the fabrication of IOLs that simulate the natural lens' accommodation, thereby reducing irritation and facilitating
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Tissue‐engineered tracheal implants: Advancements, challenges, and clinical considerations Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-22 Shixiong Wei, Yiyuan Zhang, Feixiang Luo, Kexing Duan, Mingqian Li, Guoyue Lv
Restoration of extensive tracheal damage remains a significant challenge in respiratory medicine, particularly in instances stemming from conditions like infection, congenital anomalies, or stenosis. The trachea, an essential element of the lower respiratory tract, constitutes a fibrocartilaginous tube spanning approximately 10–12 cm in length. It is characterized by 18 ± 2 tracheal cartilages distributed
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Two‐pronged reversal of chemotherapy resistance by gold nanorods induced mild photothermal effect Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-18 Qi Shang, Ziyan Chen, Jing Li, Mingmei Guo, Jiapei Yang, Zhu Jin, Yuanyuan Shen, Shengrong Guo, Feihu Wang
Chemotherapy treatment outcomes are severely restricted by multidrug resistance (MDR), in which tumors develop a multiple cross‐resistance toward drug involving the pump and nonpump resistance mechanisms, resulting in drug efflux and defending against drug toxicity. Herein, we constructed a pH and near infrared (NIR) light responsive nanomedicine DOX@FG based on gold nanorods (GNRs) that demonstrated
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Nanomaterial‐assisted oncolytic bacteria in solid tumor diagnosis and therapeutics Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-17 Xiangdi Zeng, Qi Chen, Tingtao Chen
Cancer presents a formidable challenge in modern medicine due to the intratumoral heterogeneity and the dynamic microenvironmental niche. Natural or genetically engineered oncolytic bacteria have always been hailed by scientists for their intrinsic tumor‐targeting and oncolytic capacities. However, the immunogenicity and low toxicity inevitably constrain their application in clinical practice. When
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Dissolved gases from pressure changes in the lungs elicit an immune response in human peripheral blood Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-17 Abigail G. Harrell, Stephen R. Thom, C. Wyatt Shields
Conventional dogma suggests that decompression sickness (DCS) is caused by nitrogen bubble nucleation in the blood vessels and/or tissues; however, the abundance of bubbles does not correlate with DCS severity. Since immune cells respond to chemical and environmental cues, we hypothesized that the elevated partial pressures of dissolved gases drive aberrant immune cell phenotypes in the alveolar vasculature
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Versatile tissue‐injectable hydrogels capable of the extended hydrolytic release of bioactive protein therapeutics Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-15 Eric S. Nealy, Steven J. Reed, Steven M. Adelmund, Barry A. Badeau, Jared A. Shadish, Emily J. Girard, Kenneth Brasel, Fiona J. Pakiam, Andrew J. Mhyre, Jason P. Price, Surojit Sarkar, Vandana Kalia, Cole A. DeForest, James M. Olson
Hydrogels are extensively employed in healthcare due to their adaptable structures, high water content, and biocompatibility, with FDA‐approved applications ranging from spinal cord regeneration to local therapeutic delivery. However, clinical hydrogels encounter challenges related to inconsistent therapeutic exposure, unmodifiable release windows, and difficulties in subsurface polymer insertion.
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Spatiotemporal evolutionary process of osteosarcoma immune microenvironment remodeling and C1QBP‐driven drug resistance deciphered through single‐cell multi‐dimensional analysis Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-11 Wu Xin, Ning Tang, Qiangqiang Zhao, Jianbin Xiong
The tumor immune microenvironment has manifested a crucial correlation with tumor occurrence, development, recurrence, and metastasis. To explore the mechanisms intrinsic to osteosarcoma (OS) initiation and progression, this study synthesizes multiple single‐cell RNA sequencing data sets, constructing a comprehensive landscape of the OS microenvironment. Integrating single‐cell RNA sequencing with
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Rational design of structure‐based vaccines targeting misfolded alpha‐synuclein conformers of Parkinson's disease and related disorders Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-09 Jose Miguel Flores‐Fernandez, Verena Pesch, Aishwarya Sriraman, Enrique Chimal‐Juarez, Sara Amidian, Xiongyao Wang, Caleb Duckering, Andrew Fang, Sara Reithofer, Liang Ma, Leonardo M. Cortez, Valerie L. Sim, Gültekin Tamgüney, Holger Wille
Synucleinopathies, including Parkinson's disease (PD), multiple system atrophy (MSA), and dementia with Lewy bodies (DLB), are neurodegenerative disorders caused by the accumulation of misfolded alpha‐synuclein protein. Developing effective vaccines against synucleinopathies is challenging due to the difficulty of stimulating an immune‐specific response against alpha‐synuclein without causing harmful
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A double‐edged sword: The complex interplay between engineered nanoparticles and platelets Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-06 Yathreb Asaad, Danielle Nemcovsky‐Amar, Josué Sznitman, Pierre H. Mangin, Netanel Korin
Nanoparticles (NP) play a crucial role in nanomedicine, serving as carriers for localized therapeutics to allow for precise drug delivery to specific disease sites and conditions. When injected systemically, NP can directly interact with various blood cell types, most critically with circulating platelets. Hence, the potential activation/inhibition of platelets following NP exposure must be evaluated
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Ultrasound inhibits tumor growth and selectively eliminates malignant brain tumor in vivo Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-04-02 Nitsa Buaron, Antonella Mangraviti, Yuan Wang, Ann Liu, Mariangela Pedone, Eric Sankey, Itay Adar, Abraham Nyska, Riki Goldbart, Tamar Traitel, Henry Brem, Betty Tyler, Joseph Kost
Glioma is one of the most common primary malignant brain tumors. Despite progress in therapeutic approaches, the median survival of patients with glioma remains less than 2 years, generating the need for new therapeutic approaches. Ultrasound (US) is widely used in medical fields and is used as a therapeutic tool mainly for improving the performance of therapeutic entities. In this study, we examined
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Development of a novel glucose‐dendrimer based therapeutic targeting hyperexcitable neurons in neurological disorders Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-27 Anjali Sharma, Nirnath Sah, Rishi Sharma, Preeti Vyas, Wathsala Liyanage, Sujatha Kannan, Rangaramanujam M. Kannan
Neuronal hyperexcitability and excitotoxicity lies at the core of debilitating brain disorders such as epilepsy and traumatic brain injury, culminating in neuronal death and compromised brain function. Overcoming this challenge requires a unique approach that selectively restores normal neuronal activity and rescues neurons from impending damage. However, delivering drugs selectively to hyperexcitable
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Vaccine adjuvants for infectious disease in the clinic Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-22 Morgan Goetz, Naaz Thotathil, Zongmin Zhao, Samir Mitragotri
Adjuvants, materials added to vaccines to enhance the resulting immune response, are important components of vaccination that are many times overlooked. While vaccines always include an antigen to tell the body what to vaccinate to, of equal importance the adjuvant provides the how, a significant factor in producing a complete response. The adjuvant space has been slow to develop with the first use
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Human induced pluripotent stem cells‐derived liver organoids grown on a Biomimesys® hyaluronic acid‐based hydroscaffold as a new model for studying human lipoprotein metabolism Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-16 Meryl Roudaut, Amandine Caillaud, Zied Souguir, Lise Bray, Aurore Girardeau, Antoine Rimbert, Mikaël Croyal, Gilles Lambert, Murielle Patitucci, Gaspard Delpouve, Élodie Vandenhaute, Cédric Le May, Nathalie Maubon, Bertrand Cariou, Karim Si‐Tayeb
The liver plays a key role in the metabolism of lipoproteins, controlling both production and catabolism. To accelerate the development of new lipid‐lowering therapies in humans, it is essential to have a relevant in vitro study model available. The current hepatocyte‐like cells (HLCs) models derived from hiPSC can be used to model many genetically driven diseases but require further improvement to
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Tolerability of a piezoelectric microneedle electroporator in human subjects Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-15 Chao‐Yi Lu, Pankaj Rohilla, Eric I. Felner, Gaurav Byagathvalli, Erkan Azizoglu, M. Saad Bhamla, Mark R. Prausnitz
Electroporation, or the use of electric pulses to facilitate the intracellular delivery of DNA, RNA, and other molecules, is a well‐established technique, that has been demonstrated to significantly augment the immunogenicity of DNA/mRNA vaccines and therapeutics. However, the clinical translation of traditional electroporators has been limited due to high costs, large size, complex user operation
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Evaluation of a novel vaginal cells self‐sampling device for human papillomavirus testing in cervical cancer screening: A clinical trial assessing reliability and acceptability Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-13 Chung‐Yao Yang, Ting‐Chang Chang, Hung‐Hsueh Chou, Angel Chao, Shih‐Tien Hsu, Yu‐Hsiang Shih, Huei‐Jean Huang, Cheng‐Tao Lin, Min‐Yu Chen, Lou Sun, Kuan‐Gen Huang, Kai‐Yun Wu, Wu‐Chiao Hsieh, Yi‐Ting Huang, Liang‐Hsuan Chen, Chien‐Hsing Lu, Hao Lin, Chao‐Min Cheng
Cervical cancer is a significant public health concern, particularly in low‐ and middle‐income countries where resources for prevention and treatment are limited. Routine screening, such as the Papanicolaou test (Pap smears) and human papillomavirus (HPV) testing, plays a crucial role in the early detection and prevention of cervical cancer. However, the participation rate in cervical cancer screening
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Synchronously in vivo real‐time monitoring bacterial load and temperature with evaluating immune response to decipher bacterial infection Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-13 Huaixuan Sheng, Huizhu Li, Shunyao Li, Chengxuan Yu, Yueming Wang, Haichen Hu, Lu Fang, Fuchun Chen, Yanyan Lu, Xiaogang Xu, Xing Yang, Shiyi Chen, Yuefeng Hao, Yunxia Li, Sijia Feng, Jun Chen
Determining the precise course of bacterial infection requires abundant in vivo real‐time data. Synchronous monitoring of the bacterial load, temperature, and immune response can satisfy the shortage of real‐time in vivo data. Here, we conducted a study in the joint‐infected mouse model to synchronously monitor the bacterial load, temperature, and immune response using the second near‐infrared (NIR‐II)
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Siglec15/TGF‐β bispecific antibody mediates synergistic anti‐tumor response against 4T1 triple negative breast cancer in mice Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-12 Limei Shen, Alison M. Schaefer, Karthik Tiruthani, Whitney Wolf, Samuel K. Lai
An ideal tumor‐specific immunomodulatory therapy should both preferentially target the tumor, while simultaneously reduce the immunosuppressive environment within the tumor. This guiding principle led us to explore engineering Siglec‐15 (S15) targeted bispecific antibody (bsAb) to enhance therapy against triple negative breast cancer (TNBC). S15 appears to be exclusively expressed on macrophages and
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Correction to “Primary T‐cell‐based delivery platform for in vivo synthesis of engineered proteins” Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-03-11
Radhakrishnan H, Newmyer SL, Ssemadaali MA, Javitz HS, Bhatnagar P. Primary T-cell-based delivery platform for in vivo synthesis of engineered proteins. Bioeng Transl Med. 2024; 9(1):e10605. doi:10.1002/btm2.10605 Unlike the remainder of the manuscript where the data have been developed using pan CD3 T cells, Figure 4d–f used CD4 T cells. We apologize for this error. Corrections have been disclosed
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Supramolecular dye nanoassemblies for advanced diagnostics and therapies Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-02-13 Pouria Ramezani, Stefaan C. De Smedt, Félix Sauvage
Dyes have conventionally been used in medicine for staining cells, tissues, and organelles. Since these compounds are also known as photosensitizers (PSs) which exhibit photoresponsivity upon photon illumination, there is a high desire towards formulating these molecules into nanoparticles (NPs) to achieve improved delivery efficiency and enhanced stability for novel imaging and therapeutic applications
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Engineering a “muco-trapping” ACE2-immunoglobulin hybrid with picomolar affinity as an inhaled, pan-variant immunotherapy for COVID-19 Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-02-07 Karthik Tiruthani, Carlos Cruz-Teran, Jasper F. W. Chan, Alice Ma, Morgan McSweeney, Whitney Wolf, Shoufeng Yuan, Vincent K. M. Poon, Chris C. S. Chan, Lakshmi Botta, Brian Farrer, Ian Stewart, Alison Schaefer, Jasmine Edelstein, Priya Kumar, Harendra Arora, Jeff T. Hutchins, Anthony J. Hickey, Kwok-Yung Yuen, Samuel K. Lai
Soluble angiotensin-converting enzyme 2 (ACE2) can act as a decoy molecule that neutralizes severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by blocking spike (S) proteins on virions from binding ACE2 on host cells. Based on structural insights of ACE2 and S proteins, we designed a “muco-trapping” ACE2-Fc conjugate, termed ACE2-(G4S)6-Fc, comprised of the extracellular segment of ACE2 (lacking
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Sex-dependent improvement in traumatic brain injury outcomes after liposomal delivery of dexamethasone in mice Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-02-04 Gherardo Baudo, Hannah Flinn, Morgan Holcomb, Anjana Tiwari, Sirena Soriano, Francesca Taraballi, Biana Godin, Assaf Zinger, Sonia Villapol
Traumatic brain injury (TBI) can have long-lasting physical, emotional, and cognitive consequences due to the neurodegeneration caused by its robust inflammatory response. Despite advances in rehabilitation care, effective neuroprotective treatments for TBI patients are lacking. Furthermore, current drug delivery methods for TBI treatment are inefficient in targeting inflamed brain areas. To address
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Tricaprylin-based drug crystalline suspension for intramuscular long-acting delivery of entecavir with alleviated local inflammation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-29 Min Young Jeong, Myoung Jin Ho, Joon Soo Park, Hoetaek Jeong, Jin Hee Kim, Yong Jin Jang, Doe Myung Shin, In Gyu Yang, Hye Rim Kim, Woo Heon Song, Sangkil Lee, Seh Hyon Song, Yong Seok Choi, Young Taek Han, Myung Joo Kang
In order to ensure prolonged pharmacokinetic profile along with local tolerability at the injection site, tricaprylin-based drug crystalline suspension (TS) was designed and its local distribution, pharmacokinetics, and inflammatory response, were evaluated with conventional aqueous suspension (AS). As model drug particles, entecavir 3-palmitate (EV-P), an ester lipidic prodrug for entecavir (EV),
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Engineered in vivo and in vitro tumor model recapitulates vasculogenic mimicry signatures in melanoma Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-27 Qizhi Shuai, Xinrui Xu, Yuxiang Liang, Zulala Halbiyat, Xin Lu, Zixuan Hu, Zhiwei Peng, Jie An, Zhiwei Feng, Tingjuan Huang, Hong Zhao, Zhizhen Liu, Jun Xu, Jun Xie
Vasculogenic mimicry (VM) describes a process by which tumor cells formed a novel microcirculation pattern in an endothelial cell-free manner. Clinically, VM is associated with aggressive phenotype and poor patient survival. However, the current models for investigating VM include 2D monolayer cultures, Matrigel-based cultures, and animal models, each of which has limitations. Matrigel-based models
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AI-organoid integrated systems for biomedical studies and applications Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-20 Sudhiksha Maramraju, Andrew Kowalczewski, Anirudh Kaza, Xiyuan Liu, Jathin Pranav Singaraju, Mark V. Albert, Zhen Ma, Huaxiao Yang
In this review, we explore the growing role of artificial intelligence (AI) in advancing the biomedical applications of human pluripotent stem cell (hPSC)-derived organoids. Stem cell-derived organoids, these miniature organ replicas, have become essential tools for disease modeling, drug discovery, and regenerative medicine. However, analyzing the vast and intricate datasets generated from these organoids
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Highly efficient CRISPR/Cas9-mediated exon skipping for recessive dystrophic epidermolysis bullosa Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-17 Alex du Rand, John Hunt, Christopher Samson, Evert Loef, Chloe Malhi, Sarah Meidinger, Chun-Jen Jennifer Chen, Ashley Nutsford, John Taylor, Rod Dunbar, Diana Purvis, Vaughan Feisst, Hilary Sheppard
Gene therapy based on the CRISPR/Cas9 system has emerged as a promising strategy for treating the monogenic fragile skin disorder recessive dystrophic epidermolysis bullosa (RDEB). With this approach problematic wounds could be grafted with gene edited, patient-specific skin equivalents. Precise gene editing using homology-directed repair (HDR) is the ultimate goal, however low efficiencies have hindered
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In situ light-activated materials for skin wound healing and repair: A narrative review Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-16 Jordan R. Yaron, Mallikarjun Gosangi, Shubham Pallod, Kaushal Rege
Dermal wounds are a major global health burden made worse by common comorbidities such as diabetes and infection. Appropriate wound closure relies on a highly coordinated series of cellular events, ultimately bridging tissue gaps and regenerating normal physiological structures. Wound dressings are an important component of wound care management, providing a barrier against external insults while preserving
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Micro Trojan horses: Engineering extracellular vesicles crossing biological barriers for drug delivery Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-11 Bin Zeng, Ying Li, Jiang Xia, Yin Xiao, Nawaz Khan, Bin Jiang, Yujie Liang, Li Duan
The biological barriers of the body, such as the blood–brain, placental, intestinal, skin, and air-blood, protect against invading viruses and bacteria while providing necessary physical support. However, these barriers also hinder the delivery of drugs to target tissues, reducing their therapeutic efficacy. Extracellular vesicles (EVs), nanostructures with a diameter ranging from 30 nm to 10 μm secreted
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Ultrasound molecular imaging for early detection of acute renal ischemia–reperfusion injury Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-03 Ling Ren, Yuzhuo Zhao, Tiantian Wang, Yan Tong, Ping Zhao, Fang Nie, Yukun Luo, Lianhua Zhu
Microcirculatory perfusion disorder and inflammatory response are critical links in acute kidney injury (AKI). We aim to construct anti-vascular cell adhesion molecule-1(VCAM-1) targeted microbubbles (TM) to monitor renal microcirculatory perfusion and inflammatory response.
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Feasibility of engineered Bacillus subtilis for use as a microbiome-based topical drug delivery platform Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-02 Veronica A. Montgomery, Amy J. Wood-Yang, Mark P. Styczynski, Mark R. Prausnitz
Non-adherence to medication is a major challenge in healthcare that results in worsened treatment outcomes for patients. Reducing the frequency of required administrations could improve adherence but is challenging for topical drug delivery due to the generally short residence time of topical formulations on the skin. In this study, we sought to determine the feasibility of developing a microbiome-based
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An AI-assisted integrated, scalable, single-cell phenomic-transcriptomic platform to elucidate intratumor heterogeneity against immune response Bioeng. Transl. Med. (IF 6.1) Pub Date : 2024-01-02 Christopher P. Tostado, Lucas Xian Da Ong, Joel Jia Wei Heng, Carlo Miccolis, Shumei Chia, Justine Jia Wen Seow, Yi-Chin Toh, Ramanuj DasGupta
We present a novel framework combining single-cell phenotypic data with single-cell transcriptomic analysis to identify factors underpinning heterogeneity in antitumor immune response. We developed a pairwise, tumor-immune discretized interaction assay between natural killer (NK-92MI) cells and patient-derived head and neck squamous cell carcinoma (HNSCC) cell lines on a microfluidic cell-trapping
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Trafficking through the blood–brain barrier is directed by core and outer surface components of layer-by-layer nanoparticles Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-28 Nicholas G. Lamson, Andrew J. Pickering, Jeffrey Wyckoff, Priya Ganesh, Elizabeth A. Calle, Joelle P. Straehla, Paula T. Hammond
Drug-carrying nanoparticles are a promising strategy to deliver therapeutics into the brain, but their translation requires better characterization of interactions between nanomaterials and endothelial cells of the blood–brain barrier (BBB). Here, we use a library of 18 layer-by-layer electrostatically assembled nanoparticles (NPs) to independently assess the impact of NP core and surface materials
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Growth factors and growth factor gene therapies for treating chronic wounds Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-28 James A. Mullin, Erfan Rahmani, Kristi L. Kiick, Millicent O. Sullivan
Chronic wounds are an unmet clinical need affecting millions of patients globally, and current standards of care fail to consistently promote complete wound closure and prevent recurrence. Disruptions in growth factor signaling, a hallmark of chronic wounds, have led researchers to pursue growth factor therapies as potential supplements to standards of care. Initial studies delivering growth factors
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Differential sensitivity to hypoxia enables shape-based classification of sickle cell disease and trait blood samples at point of care Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-27 Claudy D'Costa, Oshin Sharma, Riddha Manna, Minakshi Singh, Samrat Singh, Srushti Singh, Anish Mahto, Pratiksha Govil, Sampath Satti, Ninad Mehendale, Yazdi Italia, Debjani Paul
Red blood cells (RBCs) become sickle-shaped and stiff under hypoxia as a consequence of hemoglobin (Hb) polymerization in sickle cell anemia. Distinguishing between sickle cell disease and trait is crucial during the diagnosis of sickle cell disease. While genetic analysis or high-performance liquid chromatography (HPLC) can accurately differentiate between these two genotypes, these tests are unsuitable
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Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-26 Shih-Heng Chen, Hsin-Wen Wang, Pei-Ching Yang, Shih-Shien Chen, Chia-Hsin Ho, Pei-Ching Yang, Ying-Chi Kao, Shao-Wen Liu, Han Chiu, Yu-Jie Lin, Er-Yuan Chuang, Jen-Huang Huang, Huang-Kai Kao, Chieh-Cheng Huang
The prognosis for postinjury peripheral nerve regeneration remains suboptimal. Although transplantation of exogenous Schwann cells (SCs) has been considered a promising treatment to promote nerve repair, this strategy has been hampered in practice by the limited availability of SC sources and an insufficient postengraftment cell retention rate. In this study, to address these challenges, SCs were aggregated
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Photothermal Prussian blue nanoparticles generate potent multi-targeted tumor-specific T cells as an adoptive cell therapy Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-22 Elizabeth E. Sweeney, Palak Sekhri, Nethaji Muniraj, Jie Chen, Sally Feng, Joshua Terao, Samantha J. Chin, Danielle E. Schmidt, Catherine M. Bollard, Conrad Russell Y. Cruz, Rohan Fernandes
Prussian blue nanoparticle-based photothermal therapy (PBNP-PTT) is an effective tumor treatment capable of eliciting an antitumor immune response. Motivated by the ability of PBNP-PTT to potentiate endogenous immune responses, we recently demonstrated that PBNP-PTT could be used ex vivo to generate tumor-specific T cells against glioblastoma (GBM) cell lines as an adoptive T cell therapy (ATCT). In
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A bioengineered anti-VEGF protein with high affinity and high concentration for intravitreal treatment of wet age-related macular degeneration Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-19 Chengnan Huang, Yuelin Wang, Jinliang Huang, Huiqin Liu, Zhidong Chen, Yang Jiang, Youxin Chen, Feng Qian
Intravitreal (IVT) injection of anti-vascular endothelial growth factor (anti-VEGF) has greatly improved the treatment of many retinal disorders, including wet age-related macular degeneration (wAMD), which is the third leading cause of blindness. However, frequent injections can be difficult for patients and may lead to various risks such as elevated intraocular pressure, infection, and retinal detachment
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Fibroblasts and endothelial cells interplay drives hypertrophic scar formation: Insights from in vitro and in vivo models Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-20 Yaxin Tan, Mengde Zhang, Yi Kong, Fanliang Zhang, Yuzhen Wang, Yuyan Huang, Wei Song, Zhao Li, Linhao Hou, Liting Liang, Xu Guo, Qinghua Liu, Yu Feng, Chao Zhang, Xiaobing Fu, Sha Huang
Hypertrophic scar formation is influenced by the intricate interplay between fibroblasts and endothelial cells. In this study, we investigated this relationship using in vitro and in vivo models. Clinical observations revealed distinct morphological changes and increased vascularity at pathological scar sites. Further analysis using OCTA, immunohistochemistry, and immunofluorescence confirmed the involvement
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Efficacy of catheter-based drug delivery in a hybrid in vitro model of cardiac microvascular obstruction with porcine microthrombi Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-08 Yannick Rösch, Thorald Stolte, Miriam Weisskopf, Sabrina Frey, Rob Schwartz, Nikola Cesarovic, Dominik Obrist
Microvascular obstruction (MVO) often occurs in ST-elevation myocardial infarction (STEMI) patients after percutaneous coronary intervention (PCI). Diagnosis and treatment of MVO lack appropriate and established procedures. This study focused on two major points by using an in vitro multiscale flow model, which comprised an aortic root model with physiological blood flow and a microfluidic model of
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Immunogenicity of an adjuvanted broadly active influenza vaccine in immunocompromised and diverse populations Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-08 Dylan A. Hendy, Erik S. Pena, Luis Ontiveros-Padilla, Timothy A. Dixon, Denzel D. Middleton, Grace L. Williamson, Nicole Rose Lukesh, Sean R. Simpson, Rebeca T. Stiepel, Md Jahirul Islam, Michael A. Carlock, Ted M. Ross, Eric M. Bachelder, Kristy M. Ainslie
Influenza virus outbreaks are a major burden worldwide each year. Current vaccination strategies are inadequate due to antigenic drift/shift of the virus and the elicitation of low immune responses. The use of computationally optimized broadly reactive antigen (COBRA) hemagglutinin (HA) immunogens subvert the constantly mutating viruses; however, they are poorly immunogenic on their own. To increase
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Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-07 Wei-Wen Hu, Yen-Chi Chen, Chia-Wen Tsao, Shen-Liang Chen, Chung-Yuh Tzeng
A multifunctional bioreactor was fabricated in this study to investigate the facilitation efficiency of electrical and mechanical stimulations on myogenic differentiation. This bioreactor consisted of a highly stretchable conductive membrane prepared by depositing polypyrrole (PPy) on a flexible polydimethylsiloxane (PDMS) film. The tensile deformation of the PPy/PDMS membrane can be tuned by adjusting
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Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-04 Hyeon Ryoo, Hannah Kimmel, Evi Rondo, Gregory H. Underhill
Cellular phenotypes and functional responses are modulated by the signals present in their microenvironment, including extracellular matrix (ECM) proteins, tissue mechanical properties, soluble signals and nutrients, and cell–cell interactions. To better recapitulate and analyze these complex signals within the framework of more physiologically relevant culture models, high throughput culture platforms
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Establishment of bladder cancer spheroids and cultured in microfluidic platform for predicting drug response Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-12-04 Qiao Xiong, Ting Liu, Yidie Ying, Xiaowen Yu, Ziwei Wang, Hongliang Gao, Tianhai Lin, Weihua Fan, Zhensheng Zhang, Qiang Wei, Yuqing Ge, Shuxiong Zeng, Chuanliang Xu
Cisplatin-containing combination chemotherapy has been used as the standard treatment for bladder cancer patients at advanced stage. However, nearly 50% of patients are nonresponders. To guide the selection of more effective chemotherapeutic agents, a bladder cancer spheroids microfluidic drug sensitivity analysis system was established in this study. Bladder cancer spheroids were established and successfully
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Deconvolution of synthetic mRNA expression: Nucleoside chemistry alters translatability Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-11-28 Hanieh Moradian, Marko Schwestka, Toralf Roch, Manfred Gossen
Recent technological advances in the production of in vitro transcribed messenger RNA (IVT-mRNA) facilitate its clinical use as well as its application in basic research. In this regard, numerous chemical modifications, which are not naturally observed in endogenous mRNA, have been implemented primarily to address the issue of immunogenicity and improve its biological performance. However, recent findings
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First magnetic particle imaging to assess pulmonary vascular leakage in vivo in the acutely injured and fibrotic lung Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-11-29 Xin Feng, Pengli Gao, Yabin Li, Hui Hui, Jingying Jiang, Fei Xie, Jie Tian
Increased pulmonary vascular permeability is a characteristic feature of lung injury. However, there are no established methods that allow the three-dimensional visualization and quantification of pulmonary vascular permeability in vivo. Evans blue extravasation test and total protein test of bronchoalveolar lavage fluid (BALF) are permeability assays commonly used in research settings. However, they
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Therapeutic potential of mesenchymal stem cells from human iPSC-derived teratomas for osteochondral defect regeneration Bioeng. Transl. Med. (IF 6.1) Pub Date : 2023-11-29 Jiseong Kim, Jin-Su Kim, Dohyun Kim, Alvin Bacero Bello, Byoung Ju Kim, Byung-Hyun Cha, Soo-Hong Lee
Human induced pluripotent stem cells (iPSCs) hold great promise for personalized medicine, as they can be differentiated into specific cell types, especially mesenchymal stem cells (MSCs). Therefore, our study sought to assess the feasibility of deriving MSCs from teratomas generated from human iPSCs. Teratomas serve as a model to mimic multilineage human development, thus enriching specific somatic