Photodynamic therapy (PDT) using photosensitizer (PS)‐embedded silicone membrane‐covered self‐expandable metallic stents (SEMSs) can function in palliative therapeutic option for malignant gastrointestinal tract obstruction. However, stent‐related complications should be considered, and accurate delivery of light sources is technically difficult. Here, a Chlorin e6 (Ce6)‐an embedded stent‐based catheter is developed to improve its therapeutic efficacy and safety. PDT using Ce6‐embedded stent successfully induced cell death of colorectal cancer cell line. PDT‐treated liver tissues showed an increase in ablation depth in proportion to irradiation energy, and 600 J/cm2 demonstrates an even and sufficient ablation depth. Endoluminal PDT using the Ce6‐embedded stent‐based catheter was technically successful in a rat colon model without procedure‐related complications such as colonic perforation or stricture formation. The results in colonoscopy, colonography, and histological examination, along with statistical analysis, suggest that a novel PDT modality using a Ce6‐embedded stent‐based catheter was safely conducted and demonstrated apoptotic cell death at 12 h after PDT, and it gradually recovered from 2 to 4 weeks. Thus, the PDT using the Ce6‐embedded stent‐based catheter may represent a promising new approach for the treatment of malignant colorectal obstruction.

中文翻译：

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使用基于光反应支架的导管系统进行腔内光动力疗法治疗恶性结直肠梗阻


使用光敏剂 （PS） 嵌入硅胶膜覆盖的自膨式金属支架 （SEMS） 的光动力疗法 （PDT） 可用于恶性胃肠道梗阻的姑息治疗选择。然而，应考虑支架相关的并发症，并且准确输送光源在技术上很困难。在这里，开发了一种 Chlorin e6 （Ce6） - 一种基于支架的嵌入式导管，以提高其治疗效果和安全性。使用 Ce6 嵌入支架的 PDT 成功诱导了结直肠癌细胞系的细胞死亡。PDT 处理的肝组织显示消融深度与照射能量成正比，600 J/cm2 表明消融深度均匀且足够。使用 Ce6 嵌入支架的导管的腔内 PDT 在大鼠结肠模型中技术上是成功的，没有与手术相关的并发症，如结肠穿孔或狭窄形成。结肠镜检查、结肠造影和组织学检查的结果以及统计分析表明，一种使用 Ce6 嵌入支架的导管的新型 PDT 模式是安全的，并在 PDT 后 12 小时显示凋亡细胞死亡，并在 2 至 4 周内逐渐恢复。因此，使用 Ce6 嵌入支架的导管的 PDT 可能代表了治疗恶性结直肠梗阻的一种有前途的新方法。