Sperm quality analysis plays an important role in diagnosing infertility, which is widely implemented by computer‐assisted sperm analysis (CASA) of sperm‐swimming imaging from commercial phase‐contrast microscopy. A well‐equipped microscope comes with a high cost, increasing the burden of assessment, and it also occupies a large volume. For point‐of‐care testing (POCT) of sperm quality, these factors are confronted with the challenges of low‐cost and portable instruments. In this study, an encoded light‐emitting diode (LED) array illumination is employed to achieve a portable microscope with multicontrast imaging for sperm quality analysis. This microscopy has dimensions of 16.5 × 14.0 × 25.0 cm, and its dark‐field (DF) imaging provides high‐contrast sperm image data which is suitable for CASA. According to DF imaging, we developed a software of LabCASA, which can used to assess the motility characteristics of sperm. Compared with TrackMate, the difference in motility parameters from our software was less than 10% in the coefficient of variation (CV). The sperm motility parameters vary with the chamber temperature, which further confirms the reliability of our system with DF imaging. The DF imaging provides strong robustness for tracking sperm's motion under different microscopes. For assessment of the motility parameters, our system can work at a lower cost with a plastic structure. This system with DF imaging is suitable for portable POCT of sperm quality analysis, which is highly cost‐effective in resource‐constrained circumstances.

中文翻译：

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精子质量分析仪：具有暗场成像功能的便携式 LED 阵列显微镜


精子质量分析在诊断不孕症中起着重要作用，这通过商业相差显微镜对精子游泳成像的计算机辅助精子分析 （CASA） 得到广泛实施。配备齐全的显微镜成本高，增加了评估负担，而且占用的体积也很大。对于精子质量的即时检测 （POCT），这些因素面临着低成本和便携式仪器的挑战。在这项研究中，采用编码发光二极管 （LED） 阵列照明来实现具有多重对比成像的便携式显微镜，用于精子质量分析。该显微镜的尺寸为 16.5 × 14.0 × 25.0 厘米，其暗场 （DF） 成像可提供适用于 CASA 的高对比度精子图像数据。根据 DF 成像，我们开发了 LabCASA 软件，可用于评估精子的运动特性。与 TrackMate 相比，我们软件的运动参数差异在变异系数 （CV） 上小于 10%。精子活力参数随腔室温度而变化，这进一步证实了我们的 DF 成像系统的可靠性。DF 成像为在不同显微镜下跟踪精子的运动提供了强大的稳健性。为了评估运动参数，我们的系统可以使用塑料结构以较低的成本工作。该系统具有 DF 成像功能，适用于精子质量分析的便携式 POCT，在资源有限的情况下具有很高的成本效益。