Biobanks hold a pivotal role in facilitating translational and clinical research endeavors. However, the effects of prolonged storage on frozen blood samples analytes are not well defined yet. The aim of this study was to investigate the long‐term stability of the quality of DNA, RNA, and endocrine markers within blood samples amassed from the biobank over the past 11 years. The results show that the overall quality and integrity of DNA remained not significantly influenced. However, RNA integrity and purity displayed substantial deterioration as storage duration increased, to ensure high‐quality RNA for downstream analyses, advised to prioritize using blood samples stored within 3 years. Furthermore, the study examined the influence of storage time on endocrine markers. Through repeated measures ANOVA and linear regression analyses, it was evident that storage duration significantly influenced the levels of endocrine markers. This insight aids researchers in selecting appropriate markers for their investigations and augments the precision and dependability of results when dealing with long‐term stored samples.

中文翻译：

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评估长期储存对生殖生物库中生物样本的质量和完整性的影响


生物样本库在促进转化和临床研究工作中发挥着关键作用。然而，长期储存对冷冻血液样本分析物的影响尚未明确。本研究的目的是调查过去 11 年生物库收集的血液样本中 DNA、RNA 和内分泌标记物质量的长期稳定性。结果表明，DNA 的整体质量和完整性并未受到显着影响。然而，随着储存时间的延长，RNA的完整性和纯度显着恶化，为了确保下游分析的高质量RNA，建议优先使用储存在3年内的血液样本。此外，该研究还检查了储存时间对内分泌标记物的影响。通过重复测量方差分析和线性回归分析，很明显，储存时间显着影响内分泌标志物的水平。这种见解有助于研究人员为他们的研究选择合适的标记，并在处理长期存储的样本时提高结果的准确性和可靠性。