The potential of low‐intensity pulsed ultrasound (LIPUS) in regulating ovarian function has been demonstrated; however, there is a lack of scientific evidence regarding the long‐term efficacy of LIPUS in treating ovarian injury and understanding its regulatory mechanisms. In this study, 4‐vinylcyclohexene diepoxide (VCD) was used to induce ovarian injury in rats, and LIPUS was applied to target the damaged ovarian tissues. The research aimed to investigate the long‐term protective effect of LIPUS against ovum toxicity induced by VCD and elucidate the associated molecular mechanisms. During the experiment, HE staining was employed for observing the morphology and structure of the ovary, while protein sequencing was utilized for identifying and confirming the molecular mechanism through which LIPUS restores the damaged ovarian structure. The long‐term effectiveness of LIPUS in protecting against ovarian injury was evaluated through ELISA, estrous cycle monitoring, fertility testing, and behavioral analysis. The results indicated that LIPUS effectively restored the structure of damaged ovaries. Both in vivo and in vitro studies revealed that this protective effect may be attributed to LIPUS inhibiting apoptosis of ovarian granulosa cells (GCs) by regulating Daxx‐mediated ASK1/JNK signaling pathway. Subsequent functional tests demonstrated significant improvements in sex hormone secretion and regulation of estrous cycle within 6 cycles following LIPUS treatment. Additionally, there was a notable increase in offspring numbers after mating. Behavioral analysis revealed that LIPUS effectively alleviated menopausal symptoms resulting from ovarian injury including mood fluctuations, cognitive behavior changes, and reduced muscle excitability levels. These findings suggest that beneficial effects of LIPUS may help reduce VCD‐induced ovarian damage with long‐term efficacy.

中文翻译：

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低强度脉冲超声通过抑制颗粒细胞凋亡，对卵巢进行长期保护，防止 4-乙烯基环己烯二氧化酯诱导的损伤的潜力


低强度脉冲超声 （LIPUS） 在调节卵巢功能方面的潜力已得到证实;然而，缺乏关于 LIPUS 治疗卵巢损伤和了解其调节机制的长期疗效的科学证据。在这项研究中，使用 4-乙烯基环己烯二氧化物 （VCD） 诱导大鼠卵巢损伤，并应用 LIPUS 靶向受损的卵巢组织。该研究旨在探讨 LIPUS 对 VCD 诱导的卵子毒性的长期保护作用，并阐明相关的分子机制。实验中采用 HE 染色观察卵巢的形态和结构，同时利用蛋白质测序鉴定和确认 LIPUS 恢复受损卵巢结构的分子机制。通过 ELISA 、发情周期监测、生育力测试和行为分析评估 LIPUS 预防卵巢损伤的长期有效性。结果表明，LIPUS 有效恢复了受损卵巢的结构。体内和体外研究表明，这种保护作用可能归因于 LIPUS 通过调节 Daxx 介导的 ASK1/JNK 信号通路抑制卵巢颗粒细胞 （GCs） 凋亡。随后的功能测试显示，LIPUS 治疗后 6 个周期内性激素分泌和发情周期调节有显着改善。此外，交配后后代数量显着增加。行为分析显示，LIPUS 有效缓解了卵巢损伤引起的更年期症状，包括情绪波动、认知行为改变和肌肉兴奋水平降低。 这些发现表明，LIPUS 的有益作用可能有助于减少 VCD 诱导的卵巢损伤，并具有长期疗效。