The endometrium, the inner lining of the uterus, assumes a crucial role in the female reproductive system. Disorders and injuries impacting the endometrium can lead to profound consequences, including infertility and compromised women's overall health. Recent advancements in stem cell research have opened new possibilities for the treatment and repair of endometrial issues. In the present study, we constructed a degradable hydrogel by loading adipose‐derived stem cells (ADSCs) and melanin nanoparticles (MNP). In vitro cell experiments validated the biocompatibility of the prepared hydrogels and their adeptness in encapsulating ADSCs. Subsequently, we explored the impact of hydrogel@ADSC@MNP constructs in the healing process of uterine injury in mice. The results indicated that hydrogel@ADSC@MNP could augment endometrial thickness and ameliorate endometrial interstitial fibrosis. The injured tissue adjacent to hydrogel@ADSC@MNP constructs exhibited higher levels of bFGF, IGF‐1, and VEGFA compared with the corresponding tissue in mice receiving hydrogel constructs alone or in the model group. Furthermore, the hydrogel@ADSC@MNP system enhanced the proliferative capabilities of uterine endometrial cells, facilitated microvasculature regeneration, and reinstated the endometrium's capacity to receive the embryos. Our findings strongly suggest that the hydrogel@ADSC@MNP system holds significant promise for repairing and regenerating damaged endometrium.

中文翻译：

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包裹有脂肪干细胞和黑色素的温度响应水凝胶可促进子宫内膜损伤的修复和再生


子宫内膜是子宫的内壁，在女性生殖系统中发挥着至关重要的作用。影响子宫内膜的疾病和损伤可能会导致深远的后果，包括不孕和损害女性的整体健康。干细胞研究的最新进展为子宫内膜问题的治疗和修复开辟了新的可能性。在本研究中，我们通过加载脂肪干细胞（ADSC）和黑色素纳米粒子（MNP）构建了可降解水凝胶。体外细胞实验验证了所制备的水凝胶的生物相容性及其封装 ADSC 的能力。随后，我们探讨了水凝胶@ADSC@MNP结构对小鼠子宫损伤愈合过程的影响。结果表明水凝胶@ADSC@MNP可以增加子宫内膜厚度并改善子宫内膜间质纤维化。与单独接受水凝胶结构或模型组的小鼠的相应组织相比，与水凝胶@ADSC@MNP结构相邻的受损组织表现出更高水平的bFGF、IGF-1和VEGFA。此外，Hydrogel@ADSC@MNP系统增强了子宫内膜细胞的增殖能力，促进微血管再生，恢复子宫内膜接受胚胎的能力。我们的研究结果强烈表明水凝胶@ADSC@MNP系统对于修复和再生受损的子宫内膜具有重要的前景。