-
Undetected, natural conception pregnancies in luteal phase stimulations—case series and review of literature Hum. Reprod. (IF 6.0) Pub Date : 2024-07-17 B Lawrenz, B Ata, E Kalafat, R Del Gallego, S Selim, J Edades, H Fatemi
STUDY QUESTION What is the risk of an undetected natural conception pregnancy during luteal phase ovarian stimulation, and how does it impact the pregnancy's course? SUMMARY ANSWER The risk for an undetected, natural conception pregnancy in luteal phase ovarian stimulation is low and it appears that ovarian stimulation is unlikely to harm the pregnancy. WHAT IS KNOWN ALREADY Random start ovarian stimulation
-
The density of the inner cell mass is a new indicator of the quality of a human blastocyst: a valid supplement to the Gardner scoring system Hum. Reprod. (IF 6.0) Pub Date : 2024-07-16 Bo Huang, Zhou Li, Xinling Ren, Jian Bai, Jing Yue, Xiyuan Dong, Liu Yang, Bingxin Ma, Jinzhong Wang, Wenjing Zhou, Xuefeng Wang, Yaxian Guo, Keyi Si, Zhenzhi Shi, Lei Jin
STUDY QUESTION Can the density of the inner cell mass (ICM) be a new indicator of the quality of the human blastocyst? SUMMARY ANSWER The densification index (DI) developed in this study can quantify ICM density and provide positive guidance for ploidy, pregnancy, and live birth. WHAT IS KNOWN ALREADY In evaluating the quality of ICM, reproductive care clinics still use size indicators without further
-
First Australian estimates of incidence and prevalence of uterine fibroids: a data linkage cohort study 2000–2022 Hum. Reprod. (IF 6.0) Pub Date : 2024-07-16 L F Wilson, K M Moss, J Doust, C M Farquhar, G D Mishra
STUDY QUESTION What is the estimated prevalence and incidence of uterine fibroids diagnosed in Australian women of reproductive age? SUMMARY ANSWER An estimated 7.3% of Australian women had a diagnosis of uterine fibroids by the age of 45–49 years, with age-specific incidence highest in women aged 40–44 years (5.0 cases per 1000 person-years). WHAT IS KNOWN ALREADY Uterine fibroids are associated with
-
Granulosa cell metabolism at ovulation correlates with oocyte competence and is disrupted by obesity and aging Hum. Reprod. (IF 6.0) Pub Date : 2024-07-16 Atsushi Morimoto, Ryan D Rose, Kirsten M Smith, Doan T Dinh, Takashi Umehara, Yasmyn E Winstanley, Hiroaki Shibahara, Darryl L Russell, Rebecca L Robker
STUDY QUESTION Is oocyte developmental competence associated with changes in granulosa cell (GC) metabolism? SUMMARY ANSWER GC metabolism is regulated by the LH surge, altered by obesity and reproductive aging, and, in women, specific metabolic profiles are associated with failed fertilization versus increased blastocyst development. WHAT IS KNOWN ALREADY The cellular environment in which an oocyte
-
Infertility treatments and cyanotic congenital heart defects among livebirths in the USA: findings from a contemporary cohort Hum. Reprod. (IF 6.0) Pub Date : 2024-07-15 Duke Appiah, Julie Sang, Olumakinwa E Olayemi, Eric K Broni, Buse Baykoca-Arslan, Imo A Ebong, Catherine Kim
STUDY QUESTION Is there an elevated risk of cyanotic congenital heart defects (CCHD) among livebirths following infertility treatments? SUMMARY ANSWER In this population-based study of single livebirths, infertility treatment (either ART or non-ART) was associated with a higher prevalence of CCHD among livebirths. WHAT IS KNOWN ALREADY The use of infertility treatment has been on the rise over the
-
Transgender persons’ view on previous fertility decision-making and current infertility: a qualitative study Hum. Reprod. (IF 6.0) Pub Date : 2024-07-15 J D Asseler, I de Nie, F B van Rooij, T D Steensma, D Mosterd, M O Verhoeven, M Goddijn, J A F Huirne, N M van Mello
STUDY QUESTION How do adult transgender and gender diverse (TGD) people, who are infertile due to prior gender-affirming treatment, view their current infertility and their reproductive decisions made in the past? SUMMARY ANSWER In a time where sterilization was mandatory, transgender adolescents prioritized gender-affirming treatment over their future fertility and would make the same choice today
-
The impact of type 2 diabetes on polycystic ovary syndrome in patients undergoing sleeve gastrectomy Hum. Reprod. (IF 6.0) Pub Date : 2024-07-15 Tao Zhu, Yian Zhao, Xiaocheng Bi, Peikai Zhao, Teng Liu, Yuxuan Li, Shumin Li, Shigang Zhao, Shaozhuang Liu, Xin Huang
STUDY QUESTION Does the concurrent type 2 diabetes mellitus (T2DM) aggravate the features and prognosis of PCOS in patients undergoing sleeve gastrectomy (SG)? SUMMARY ANSWER For patients undergoing SG with obesity, concurrent T2DM is associated with aggravated metabolic but milder reproductive features of PCOS and did not attenuate the resumption of regular menstruation for up to 1 year after surgery
-
Pretreatment with luteal estradiol for programming antagonist cycles compared to no pretreatment in advanced age women stimulated with corifollitropin alfa: a non-inferiority randomized controlled trial Hum. Reprod. (IF 6.0) Pub Date : 2024-07-15 Isabelle Cédrin-Durnerin, Isis Carton, Nathalie Massin, Nicolas Chevalier, Sophie Dubourdieu, Bettina Bstandig, Xénia Michelson, Seydou Goro, Camille Jung, Anne Guivarc’h-Lévêque
STUDY QUESTION Does luteal estradiol (E2) pretreatment give a similar number of retrieved oocytes compared to no-pretreatment in advanced-aged women stimulated with corifollitropin alfa in an antagonist protocol? SUMMARY ANSWER Programming antagonist cycles with luteal E2 gave similar number of retrieved oocytes compared to no-pretreatment in women aged 38–42 years. WHAT IS KNOWN ALREADY Programming
-
Perspectives on sperm donor anonymity: insights from donor-conceived adults in Belgium Hum. Reprod. (IF 6.0) Pub Date : 2024-07-15 Phyline Casteels, Julie Nekkebroeck, Herman Tournaye
STUDY QUESTION Are donor-conceived adults in Belgium interested in obtaining donor information, and do these interests vary based on their family backgrounds? SUMMARY ANSWER Donor-conceived adults express a significant interest in obtaining donor-related information, with the highest interest reported by offspring from heterosexual couples compared to those from lesbian couple-parented or single-parent
-
O-130 The spatial conformational features of blastocysts can serve as a new basis for selection Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 B Huang, M Binxing, J Lei
Study question Can spatial conformational features become a new basis for blastocyst selection? Summary answer The spatial conformational features of blastocysts can be associated with clinical outcomes, which is a new basis for blastocyst selection. What is known already At present, the quality evaluation of blastocysts is two-dimensional, and there are relatively few evaluation indicators. There
-
O-075 Particulate matter (PM2.5 and PM10) exposure prior to oocyte collection is associated with decreased live birth rates in subsequent frozen embryo transfers Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 S Leathersich, C Roche, M Walls, E Nathan, R Hart
Study question Does exposure to particulate matter air pollution prior to oocyte collection (OPU) or frozen embryo transfer (FET) impact FET live birth rates? Summary answer Increasing PM10 and PM2.5 exposure prior to OPU is associated with decreased odds of live birth in subsequent FET, independent of the conditions at FET. What is known already Ambient air pollution is increasing, and exposure is
-
Care-seeking for difficulties conceiving in sub-Saharan Africa: findings from population-based surveys in eight geographies Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 Suzanne O Bell, Elizabeth Larson, Dory Bittle, Caroline Moreau, Elizabeth Omoluabi, Funmilola M OlaOlorun, Pierre Akilimali, Simon P S Kibira, Fredrick Makumbi, Georges Guiella, Rosine Mosso, Peter Gichangi, Philip Anglewicz
STUDY QUESTION What is the nature of women’s care-seeking for difficulties conceiving in sub-Saharan Africa (SSA), including the correlates of seeking biomedical infertility care at a health facility? SUMMARY ANSWER Care-seeking for difficulties getting pregnant was low, much of which involved traditional or religious sources of care, with evidence of sociodemographic disparities in receipt of biomedical
-
O-091 Severe maternal and neonatal morbidity among gestational carriers: a population-based cohort study Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 M Ivanova, J Ray, J Shellenberger, J Pudwell, M P Velez
Study question Are gestational carriers at higher risk of severe maternal (SMM) or neonatal (SNM) morbidity, compared to patients using unassisted conception or in vitro fertilization (IVF)? Summary answer A higher risk of SMM and adverse pregnancy outcomes is seen among gestational carriers, without evident neonatal morbidity. What is known already The use of gestational carriers (GC) also known as
-
O-315 Familial endocrine disease increases the risk of pregnancy loss and recurrent pregnancy loss– a nationwide register-based study of 366,548 Danish women Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 P Egerup, S Bliddal, D Westergaard, D Glintborg, S Bonnema, U Feldt-Rasmussen, M Nyegaard, L H Mortensen, H S Nielsen
Study question Is endocrine disease and pregnancy loss associated, and can the association be due to inherited genetic causes? Summary answer Endocrine disease is associated with an increasing number of pregnancy losses. Endocrine disease in parents and siblings increases a woman’s risk of pregnancy loss. What is known already One in four pregnancies results in a pregnancy loss, but only 60% hereof
-
O-027 Efficacy results from the phase II randomized clinical trial: OXO-001 in infertile women undergoing egg donation IVF/ICSI Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 A Arbat, J Bellver, J Garcia-Velasco, H Višňová, R Kurzawa, A Gosalvez, N P Polyzos, J J Espinós, R Trinchant, M Llorens, R Torres, I Canals
Study question Does OXO-001 increase implantation and pregnancy rates in women undergoing egg donation IVF/ICSI and embryo transfer (ET) compared to placebo? Summary answer This proof-of-concept (PoC) prospective randomized and controlled study shows that OXO-001 treatment increases implantation and pregnancy rates in infertile women undergoing IVF/ICSI with studied doses. What is known already OXO-001
-
O-158 Social disparities in achieving a live birth after initiating ART treatment: a national register-based study among women in Denmark Hum. Reprod. (IF 6.0) Pub Date : 2024-07-10 R Uggerhøj, A Skaaning, F Kyhl, L Schmidt, D Vassard
Study question How are educational level, labor market attachment and income associated with achieving a live birth after initiating ART treatment among women in Denmark? Summary answer Higher educational level, labor market attachment and higher income were strongly associated with achieving a live birth after initiating ART treatment among women in Denmark. What is known already Infertility is increasing
-
Non-invasive preimplantation genetic testing for aneuploidy: is the promise real? Hum. Reprod. (IF 6.0) Pub Date : 2024-07-06 Michelle Volovsky, Richard T Scott, Emre Seli
Recent advances in preimplantation genetic testing for aneuploidy (PGT-A) have significantly enhanced its application in ART, providing critical insights into embryo viability, and potentially reducing both the time spent in fertility treatments and the risk of pregnancy loss. With the integration of next-generation sequencing, PGT-A now offers greater diagnostic precision, although challenges related
-
Longitudinal associations between urinary biomarkers of phthalates and replacements with novel in vivo measures of placental health Hum. Reprod. (IF 6.0) Pub Date : 2024-07-06 Emma M Rosen, Danielle R Stevens, Erin E McNell, Mollie E Wood, Stephanie M Engel, Alexander P Keil, Antonia M Calafat, Julianne Cook Botelho, Elena Sinkovskaya, Ann Przybylska, George Saade, Alfred Abuhamad, Kelly K Ferguson
STUDY QUESTION What is the longitudinal association between gestational phthalate exposure and in vivo placental outcomes? SUMMARY ANSWER Phthalates were adversely associated with placental microvasculature, stiffness, and presence of calcification, with different metabolites associated with different outcomes. WHAT IS KNOWN ALREADY Phthalate exposure is ubiquitous and implicated as a contributor to
-
Moonshot. Long shot. Or sure shot. What needs to happen to realize the full potential of AI in the fertility sector? Hum. Reprod. (IF 6.0) Pub Date : 2024-07-04 Gerard Letterie
Quality healthcare requires two critical components: patients’ best interests and best decisions to achieve that goal. The first goal is the lodestar, unchanged and unchanging over time. The second component is a more dynamic and rapidly changing paradigm in healthcare. Clinical decision-making has transitioned from an opinion-based paradigm to an evidence-based and data-driven process. A realization
-
P-742 In-vitro fertilization outcomes after laparoscopic repair of cesarean scar defect Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S Y Shin, K Hwang, J E Shin, D H Choi, J H Kim
Study question Does laparoscopic repair of cesarean scar defect improve in-vitro fertilization (IVF) outcomes? Summary answer Laparoscopic (LSC) repair of cesarean scar defect (CSD) should selectively indicated to patients experiencing repetitive pregnancy failure or having an increased depth of niche What is known already Cesarean section (CS) rate has markedly from 7% in 1990 to an estimated 29%
-
P-269 Three-dimensional assessment of the inner cell mass is more effective at predicting implantation and live birth than Gardner grading Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 R Morgan, C He, C Jacques, T Ferrand, T Fréour, C Hickman, N Zaninovic, J Malmsten
Study question 3-dimensional quantitative assessment of the inner cell mass (ICM) is more effective at selecting viable embryos than the widely used Gardner grading system? Summary answer 3-dimensional quantitative assessment of the ICM is significantly more predictive of implantation and live birth than the widely used Gardner grading of the ICM What is known already ICM assessment forms a key part
-
P-337 Ready for polygenic risk scores? An analysis of regulation of preimplantation genetic testing in seven European countries Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 M Siermann, V Van der Schoot, E Bunnik, P Borry
Study question Would the different regulatory approaches for preimplantation genetic testing (PGT) in Europe permit the implementation of preimplantation genetic testing using polygenic risk scores (PGT-P)? Summary answer While the regulatory approaches for PGT differ between countries, the latitude for potential implementation of PGT-P seems limited in all three regulatory models. What is known already
-
O-128 Can embryo re-expansion pattern post-biopsy for PGT-A have a predictive value on the outcome? Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 I Elkhatib, A Bayram, A Abdala, R Gonzales, D G Raquel, L Melado, B Lawrenz, E Kalafat, D Nogueira, H Fatemi
Study question Does re-expansion grade at 1 hour post-biopsy correlate with post-thawing re-expansion rate and live birth rate? Summary answer Embryo re-expansion 1-hour post-biopsy is associated with post-warming re-expansion and increased live birth. What is known already Blastocyst cryopreservation is a key component of PGT-A procedures. In non-PGT-A cycles, collapsing blastocysts before vitrification
-
O-219 Explainable artificial intelligence (XAI) to determine the follicle sizes on trigger day that maximize mature oocyte yield Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S Hanassab, S M Nelson, A Akbarov, A C Yeung, A Hramyka, T Alhamwi, R Salim, A N Comninos, G H Trew, T W Kelsey, T Heinis, W S Dhillo, A Abbara
Study question Which follicle sizes on trigger day (TD) are key to obtaining mature oocytes, embryos, and blastocysts, thereby enhancing live birth rates? Summary answer Follicles sized 13-18mm are most likely to yield mature oocytes. Live birth rate (LBR) was increased with a greater proportion of follicles in this range. What is known already At the time of oocyte retrieval, follicles that are either
-
P-146 The importance of cytoplasmic strings between inner cell mass and trophectoderm after blastulation on embryonic development and pregnancy outcome Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 J Zhu, W Han, G Huang
Study question Whether the cytoplasmic strings between inner cell mass and trophectoderm during blastocyst expansion have a significant impact on embryo development and pregnancy outcomes? Summary answer The presence of cytoplasmic strings is related to morphological quality, but it has no impact on embryo ploidy or clinical outcome after euploid embryo transfer. What is known already Cytoplasmic strings
-
P-126 The impact of ICSI versus IVF on the reproductive outcomes of couples with non-male factor infertility and frozen-thawed embryo transfer cycles Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 Y Zhang, G Yichun
Study question Does Intracytoplasmic Sperm Injection (ICSI) improve reproductive outcomes in patients with non-male factor infertility undergoing Frozen-Thawed Embryo Transfer (FET) treatment? Summary answer ICSI showed slightly improved clinical outcomes compared to IVF, but was associated with higher preterm birth rates and low birthweight when twins were born. What is known already The use of ICSI
-
P-536 Preimplantation Genetic Testing for hereditary breast and ovarian cancer syndrome due to BRCA pathogenic variant : the parisian center experience Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 R Borghese, J Steffann, O Lassartesse, F Souare, N Achour, A Mayeur, A Benachi, D Stoppa Lyonnet, M Grynberg, C Sonigo
Study question What are the outcomes of Preimplantation genetic testing for monogenic disease (PGT-M) for BRCA pathogenic variants? Summary answer Among couples entering a PGT-M program for BRCA pathogenic variants, 23.5% achieved a live-birth. What is known already BRCA pathogenic variants are associated with an oncologic predisposition with variable penetrance and are accessible to screening and
-
O-194 Cumulative live birth and neonatal outcomes according to different male factors in IVF/ICSI cycles Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 L Jiang, K Qian
Study question What are the impacts of different male factors on cumulative live birth and neonatal outcomes of IVF/ICSI cycles? Summary answer Severe male infertility factors may be negatively associated with fertilization and egg utilization, embryo development and cumulative live birth rate, but not with neonatal outcomes. What is known already Several studies have explored the effect of semen quality
-
P-410 A modified artificial cycle-frozen embryo transfer(mAC-FET) protocol with evidence of corpus luteum(CL) formation as determined by serum relaxin-2 in early pregnancy :a prospective proof-of-concept study Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 M Varma, S Singh, H Bansal, H Tuli, R Bansal, T Kaur
Study question Can a mAC-FET protocol for endometrial preparation in ovulatory patients lead to formation of a CL as evidenced by serum relaxin-2 in the pregnant patients? Summary answer CL forms in the mAC-FET protocol as serum relaxin-2 in early pregnancy was comparable to that in spontaneous conception.Late luteal rise of relaxin-2 wasn’t demonstrated. What is known already Clinical evidence points
-
P-566 Addressing ethnic underrepresentation in carrier screening: prioritizing comprehensive variant curation over ClinVar-based automated analysis Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 I Trujillo, A Feliu, M Palahi, A Martín, R Moret, D Cotán, M Sandalinas
Study question How does the use of a ClinVar-based approach in Carrier Genetic Screening (CGS) affect the efficiency and accuracy of carrier detection analysis? Summary answer For CGS, comprehensive variant curation, not automated analysis alone, addresses risk from under-representation of diverse populations in genetic databases. What is known already Next-generation sequencing (NGS) analyses in Carrier
-
P-101 Mitochondria-mediated apoptosis induced testicular dysfunction in bisphenol F treated rats: ameliorative effect of baicalin Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 M Hamed, A Odetayo
Study question Will baicalin treatment ameliorate bisphenol F (BPF)-induced testicular dysfunction? Summary answer Baicalin ameliorates BPF-induced mitochondrial dysfunction-associated apoptosis What is known already BPF is a chemical presently considered a major replacement for bisphenol A and has been widely used in the canning and other food processing industries. However, a major limitation of
-
O-013 Population pharmacokinetics of follitropin delta during ovarian stimulation Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 P Heiser, P Pinton, D Jonker
Study question Which patient characteristics affect serum follicle stimulating hormone (FSH) concentrations in women undergoing ovarian stimulation (OS) with follitropin delta, a human cell line derived FSH? Summary answer Body weight is the main determinant of serum FSH concentrations during OS. Renal function, race, ethnicity, hepatic function and age have at most small influence. What is known already
-
P-761 Four-dimensional ultrasound using an electronic probe to guide embryo transfer: a feasibility study Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 D Vasquez Carlon, J P Rouleau, J Guerrero, A Rodriguez, M Costa, J Hernandez, F Naftolin, A Palumbo
Study question Is four-dimensional ultrasound (4D-US) guided embryo transfer feasible and safe? Summary answer 4D trans-abdominal US with electronic probe is feasible and safe and justifies a formal study for non-inferiority to two-dimensional ultrasound (2D-US) for embryo transfer guidance. What is known already The use of 2D-US to guide embryo transfer is the usual procedure (Eshre/ASRM guidelines
-
O-159 Domestic and cross-border reproductive care among same-sex male intended parents: comparing live birth rates (LBRs) in a national retrospective cohort in the United States (U.S.) Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 B Monseur, L Shandley, J Zhang, S Leonard, B Laniakea, H Hipp, J Obedin-Maliver, R Alvero, M Leondires
Study question How common is cross-border reproductive care (CBRC) in the U.S. among international same-sex male intended parents? Do CBRC LBRs differ compared to domestic counterparts? Summary answer A substantial number of international same-sex male intended parents seek CBRC in the U.S. LBRs per transfer are reassuring and comparable to domestic counterparts. What is known already Same-sex male
-
O-322 What is the number of IVF cycles needed to produce at least one euploid blastocyst in patients of all ages with varied AMH levels Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 Y Alhelou, M Fakih
Study question How many cycles are needed to get at least one blastocyst in patients of different AMH levels and varied ages? Summary answer Age and AMH determine the number of cycles, which ranges from 1 cycle in young patients up to 10 cycles in over-44-year-old patients. What is known already AMH secretion by ovarian granulosa cells initiates during the 36th week of gestation in pre-antral and antral
-
P-473 Assessing patient perceptions: emotional support and clinic culture in fertility care. Examining changes in emotional support and clinic culture over 4 years Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S Banks
Study question How do patients perceive emotional support and clinic culture in the context of fertility care, what factors contribute to their overall experiences within fertility clinics? Summary answer Patients find clinic support lacking, rated 4.8/10, with <45% perceiving a supportive clinic culture. Despite increased awareness, emotional support perception declined from 2019 to 2023. What is
-
P-551 Application of simultaneous PGT-M/PGT-A using whole genome sequencing and haplotyping for monogenetic disorders, multiple variants, low penetrance, and de novo disorders Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S Madjunkova, A Agrawal, S Chen, R Abramov, N Logan, K Glass, A Baratz, P Sharma, M Madjunkov, C Librach
Study question Is the concurrent use of high-resolution PGT-A and genome-wide haplotyping with disease-specific assays for PGT-M effective in an unselected patient population at-risk for monogenetic disorders? Summary answer PGT-A/PGT-M is an effective treatment for couples/patients burdened with genetic diseases, that provides optimal family planning, prevents vertical transmission, and reduces personal
-
O-017 Swim-up versus density gradients for sperm preparation in intrauterine insemination: a multi-centre randomised clinical trial Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 T Duong, T K Le, A TL Vu, D L Nguyen, T D Pham, M T Nguyen, P TM Nguyen, T M Nguyen, A H Le, C T Tran, V Q Dang, L N Vuong, B W Mol, R J Norman, T M Ho
Study question What is the effectiveness of swim-up (SU) and density gradients (DG) for sperm preparation in infertile couples undergoing intrauterine insemination (IUI)? Summary answer In couples undergoing IUI, there is no significant difference in the treatment outcomes between SU and DG. What is known already Swim-up (SU) and density gradients (DG) are the most two commonly used techniques for
-
P-187 Profiling secreted proteins in human embryos: Time-specific discrimination of ploidy and confirmation of enhanced post-implantation development with maternal co-culture Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 A Palomar, A Quiñonero, R Yagüe-Serrano, S Morales-Alvarez, M Molè, M Florensa, A Mercader, F Domínguez, I Sánchez-Ribas
Study question Can proteins released by embryos into culture medium between 7- and 14-days post-fertilization (d.p.f.) potentially function as biomarkers indicating the ploidy status of the embryos? Summary answer Combined secreted levels of calreticulin (CALR), human Placental Lactogen (hPL), Placental Growth Factor (PlGF) and human Pappalysin-1 (PAPP-A) differentiate euploid from chromosome 21-aneuploid
-
P-638 Paternal Consumption of Caloric and Non-Caloric Sweeteners is Associated with Sex-Specific Differences in Reproductive outcomes in offspring Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 M Kearns, C Reynolds
Study question Does paternal pre-conception consumption of caloric or non-caloric sweeteners (NCS) coupled with high fat diets (HFD) predispose offspring to early puberty onset in mice? Summary answer Paternal prenatal consumption of the NCS Rebaudioside A(RebA) resulted in early puberty onset in female offspring only compared to water and caloric sweetener groups. What is known already A growing body
-
P-311 Impact of adenomyosis on reproductive outcomes applying the revised Morphological Uterus Sonographic Assessment criteria in fresh donor oocyte recipients pretreated with GnRH agonist Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S Peralta, P Carrillo, Y Barral, C Ros, M Mendez, A Goday, D Manau
Study question Could the diagnosis of adenomyosis (AD) impact reproductive outcomes when applying the revised Morphological Uterus Sonographic Assessment (MUSA) criteria in fresh donor oocyte cycles? Summary answer Adenomyosis diagnosis following the revised MUSA criteria in recipients did not have adversely affect reproductive outcomes in fresh donor oocyte recipients pretreated with GnRH agonist
-
P-232 EIF4E1B Contributes to Early Translation Burst for Embryogenesis Whose Deficiency Causes Embryo Development Arrest at Four-Cell Stage during Oocyte to Embryo Transition in Women Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 G Jing, L Ge
Study question What are the effects and corresponding mechanisms of the early burst of translation inhibited during the oocyte-to-embryo transition (OET), which is essential for human embryogenesis? Summary answer EIF4E1B contributes to the early burst of translation for embryogenesis whose deficiency causes embryo development arrest at four-cell stage during OET in women. What is known already The
-
P-738 Reproductive surgery for fertility preservation: Robotic single-port myomectomy (da Vinci® SP) versus robotic single-site myomectomy (da Vinci® Xi or Si) Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 K Jeong, J H Lee, S Y Park, W Shin, H W Chung
Study question To compare clinical and surgical outcomes of robotic single-port myomectomy using da Vinci® SP and robotic single-site myomectomy with Xi or Si for fertility preservation. Summary answer The robotic single-port myomectomy (da Vinci® SP) might be feasible, even if the myoma is deep-seated, compared to robotic single-site myomectomy (Xi or Si system). What is known already Myomas are clinically
-
P-418 What is the optimal midluteal phase circulating progesterone level during a fresh IVF cycle? The answer differs from what one expects in a natural cycle Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S R Soares, I Costa Vieira, A R Neves, S Santos Ribeiro
Study question Do midluteal phase circulating progesterone (P4) levels relate to the fresh embryo transfer clinical outcome and is rescue P4 useful in such cases? Summary answer Live birth rate (LBR) was significantly lower when P4 levels were below 20ng/ml and did not seem to benefit from rescue intramuscular P4. What is known already Preceding literature on the predictive value of midluteal P4 for
-
P-319 Chronic endometritis prevalence, associated factors and pregnancy outcome among infertile women Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 H Lee, J Kim, D Choi, C Park, H Kwon, J Kim, J Shin
Study question What are the prevalence, associated factors and pregnancy outcome of chronic endometritis(CE) among infertile women? Summary answer The overal prevalence of chronic endometritis in infertile women was 24.7%. Diagnosis rate of CE was higher in primary infertility compared to other infertility groups. What is known already Chronic endometritis is defined as a chronic inflammation of uterine
-
P-406 Late paternal effect rooted in sperm DNA double-strand breaks (DSBs) correlates with the likelihood of miscarriage in patients with poor ovarian response Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 M S Lee, C I Lee, C H Chen, C C Huang, H M Tsao, T A Chang, C T Hsu, H C Chang, T E Wang
Study question Can paternal risk factors be minimized and prevented in ICSI procedure? Summary answer The influence of DSBs can be prevented in ICSI cases with normal ovarian response, whereas DSB is exclusively associated with miscarriage in poor ovarian reserve. What is known already Sperm DNA fragmentation (SDF) is intricately linked to male fertility capacity, with a high prevalence affecting 20%
-
P-316 Fertility, pregnancy outcomes, and perinatal results related to experimental endometriosis in a murine model Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 N Franzoni Smargiassi, C Mantovani da Luz, J C Rosa e Silva, P A Navarro
Study question Does the transplantation of endometrial tissue fragments into the peritoneal wall of female mice reduce natural fertility and lead to compromised obstetric and perinatal outcomes? Summary answer Experimental murine endometriosis did not compromise fertility, but females with more ectopic endometria had litters with reduced birth weight and fewer offspring at weaning. What is known already
-
P-204 Automated embryo assessment: a simple web application correlates scores with embryo morphology, developmental velocity, ploidy, and clinical outcomes Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 C Albert Rodriguez, L Bori, L Murria, C Piluso, J Remohí, M Meseguer
Study question How does the novel automated scoring system in our laboratory correlate with treatment success in different subpopulations? Summary answer The web app automates embryo assessment, linking scores to morphology, development speed, ploidy, and clinical outcomes in treatments with both patient and donor oocytes. What is known already In the realm of assisted reproductive technologies, numerous
-
P-592 Comparison of the cumulative live birth rate of different doses of rFSH in poor ovarian reserve women undergoing GnRH antagonist ART treatment: a Randomized Controlled Trail Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 L Xiaofeng, T Yi, G Fei
Study question Does an increased FSH dosage result in higher cumulative live birth rates in advanced age women with poor ovarian reserve treated with IVF or ICSI? Summary answer In poor ovarian reserve women, FSH dose of 300 IU/day does not improve cumulative live birth rates as compared to 150 IU/day. What is known already Natural fecundity and pregnancy rate following ART decrease dramatically in
-
P-699 High levels of total FSH during COS is detrimental to oocyte development in good responders: results from a three-year retrospective, multicenter study Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 H Karimova, N Pylypenko, D Hudkova, O Kotlyarova, V Vozniuk, L Barbakadze, B Aydin, U Dorofeyeva
Study question Are elevated doses of follicle stimulating hormone (FSH) detrimental to oocyte maturation and the efficacy of ovarian stimulation in good responders? Summary answer Elevated FSH levels reduce the number of oocytes collected from good responders. Patients receiving lower total FSH doses had optimal and higher maturation rates. What is known already There is an inverse relationship between
-
P-422 Modified natural cycle does not increase live birth rates compared to hormone replacement therapy: success rates and perinatal outcomes of 1.354 single euploid blastocyst cryotransfers Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 A Garcia-Faura, K Portela, B Freijomil, A Mallafre, A Lopez, F Garcia, V Montalvo, B Marques
Study question Frozen euploid single blastocyst transfer in modified natural cycle (mNC) increases success rates, improving obstetric outcomes compared to hormone replacement therapy (HRT) protocols? Summary answer Elective euploid single-blastocyst transfer (eeSBT) in mNC did not show higher success rates nor better obstetric and perinatal outcomes compared to HRT protocols What is known already eeSBT
-
P-162 Embryo transfer impact: a comprehensive national cohort analysis comparing maternal outcomes across varied embryo stages in fresh and frozen transfers Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 C T Chang, E M Tsai, H Y Chuang, I L Hsu, S F Weng
Study question What is the ideal choice between fresh and frozen embryos transfer, and should the transfer be done at the cleavage or blastocyst stage? Summary answer This study highlights the clinical pregnancy and live birth superiority of frozen embryo transfer in blastocysts but not in cleavage stage embryos. What is known already Frozen embryo transfer not only improves reproductive outcomes by
-
P-362 Cellular senescence in mouse ovaries as an explanatory mechanism of cyclophosphamide detrimental effect to female fertility Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 X Hao, J Zhao, K Rodriguez-Wallberg
Study question Is cellular senescence a causative mechanism of cyclophosphamide’s detrimental effect to female fertility? Summary answer Cyclophosphamide induced up-regulation of genes related to cellular senescence and associated pathways, oocyte organelles alterations and increased beta-galactosidase (b-Gal) positive cells in mouse ovaries. What is known already Cyclophosphamide (CPA) is frequently
-
P-351 Reproductive outcomes in a cohort of lymphoma patients Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 L B Colmorn, A Tønnes Pedersen, E C Larsen, S G Kristensen, K T Macklon
Study question What are the reproductive outcomes in women with malignant lymphoma Hodgkins (HD) and non-Hodgkins (NH) from a single, experienced center? Summary answer 74 children were born to 43 women (28 with HD, 15 with NH) giving a live birth rate (LBR) of 81% and 72%, respectively. What is known already The risk of premature ovarian insufficiency (POI) after HD and NH is related to female age
-
P-508 Management of incidental findings after preconception expanded carrier screening: identifying genetic predispositions to cancer and metabolic conditions in 8010 screened ART patients Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 G López, J J Guillén, A Bellés López, A Sánchez Díaz, A Domingo-Gallego, X Armengol, M Segura-Puimedon, L Armengol-Dulcet, R Buscà, D Mataró, M Popovic, I Miguel-Escalada, A Rodriguez-Aranda
Study question How do individuals and fertility clinics navigate incidental findings from Expanded Carrier Screening (ECS) in reproductive decision-making? Summary answer Incidental ECS findings prompted varied responses from patients and gamete donors, influencing diverse reproductive choices and underscoring the pivotal role of pre-test genetic counseling. What is known already ECS aims to assess
-
P-182 High-resolution synthetic embryo images generated by AI Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 H J Chae, J H Park, M K Chung, M Kim, H S Min, N Y Kim, D H Yoo, I Oh, H Lee
Study question How effectively can a latent diffusion model generate high-fidelity embryo images tailored to the specific contextual needs of researchers, based on the user’s text input? Summary answer The latent diffusion model successfully generated high-resolution embryo images, providing a novel approach to address data scarcity in embryo imaging. What is known already The use of AI in generating
-
P-533 Impact on clinical outcomes of day-6 extended culture and reduced culture media volume applied to non-invasive PGT-A Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 C Rubio LLuesa, L Navarro-Sánchez, G Ardestani, G Barroso, C Bisioli, K Boynukalin, D Cimadomo, G Mamede Andrade, D Valbuena, C Simon-Valles, D Sakkas
Study question Impact on clinical outcomes of day-6 extended culture and reduced culture media volume applied to non-invasive PGT-A Summary answer The implementation of changes in culture conditions to accommodate niPGT-A has no impact on blastocysts viability or pregnancy outcomes. What is known already The recent identification of embryo cell-free DNA (cfDNA) in the culture media has created the
-
P-223 Follicular fluid from women stimulated with hMG protocol displays altered energy metabolism and this impacts embryo morphokinetics Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 S Fortini, M Del Collado Barrondo, C Giovanna Basso, D Ejzenberg, N Frantz, R G B Sousa, M Nichi, M P Milazzotto
Study question Does different type of ovarian stimulation affect follicle metabolism? Summary answer Patients stimulated hMG-only show increased levels of glucose and MDA in follicular fluid and longer Cc3 embryo time compared to patients stimulated rFSH and hMG. What is known already Oocyte growth and maturation strongly rely on energy metabolism. However, there is a notable lack of studies examining
-
P-758 Perinatal deaths to women dispensed clomiphene citrate: A study within a whole of population birth cohort Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 M Davies, A Rumbold, R Fernandez, H McElroy, V Moore
Study question Is the risk of stillbirth and neonatal death increased in pregnancies where clomiphene citrate was dispensed 3 months before or after time of conception? Summary answer The risk of stillbirth and/or neonatal death is 45% higher in pregnancies among women dispensed clomiphene citrate compared to unexposed women independent of confounders. What is known already Clomiphene citrate (CC)
-
P-359 Ten years’ experience using cryopreserved oocytes from In Vitro Maturation (IVM) or Controlled Ovarian Hyperstimulation (COH) cycles for fertility preservation in oncologic indications Hum. Reprod. (IF 6.0) Pub Date : 2024-07-03 Y Boumerdassi, V Puy, S Sarandi, J Lousqui, L Sayyahensan, M Majer, J Eid, C Vinolas, R Goldstein, A Loiseau, F Eustache, M Grynberg, M Peigné, D Nogueira, C Sifer
Study question Is IVM a relevant option for fertility preservation in cancer patients, considering biological/clinical outcomes post-thawing, compared to COH? Summary answer This study shows that despite a significantly altered embryo morphology within the IVM group, the clinical outcomes remain comparable to those of COH. What is known already Fertility Preservation (FP) techniques are proposed to