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Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-27 Jiwei Mao, Hongyu Zhang, Yu Chen, Liang Wei, Jun Liu, Jens Nielsen, Yun Chen, Ning Xu
Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic
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Advancing microbial production through artificial intelligence-aided biology Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-24 Xinyu Gong, Jianli Zhang, Qi Gan, Yuxi Teng, Jixin Hou, Yanjun Lyu, Zhengliang Liu, Zihao Wu, Runpeng Dai, Yusong Zou, Xianqiao Wang, Dajiang Zhu, Hongtu Zhu, Tianming Liu, Yajun Yan
Microbial cell factories (MCFs) have been leveraged to construct sustainable platforms for value-added compound production. To optimize metabolism and reach optimal productivity, synthetic biology has developed various genetic devices to engineer microbial systems by gene editing, high-throughput protein engineering, and dynamic regulation. However, current synthetic biology methodologies still rely
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Functional roles and engineering strategies to improve the industrial functionalities of lactic acid bacteria during food fermentation Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-21 Huan Yang, Liying Hao, Yao Jin, Jun Huang, Rongqing Zhou, Chongde Wu
In order to improve the flavor profiles, food security, probiotic effects and shorten the fermentation period of traditional fermented foods, lactic acid bacteria (LAB) were often considered as the ideal candidate to participate in the fermentation process. In general, LAB strains possessed the ability to develop flavor compounds via carbohydrate metabolism, protein hydrolysis and amino acid metabolism
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Development and applications of lipid hydrophilic headgroups for nucleic acid therapy Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-19 Wanting Ma, Xingxing Fu, Tianyi Zhao, Yanfei Qi, Shubiao Zhang, Yinan Zhao
Nucleic acid therapy is currently the most promising method for treating tumors and genetic diseases and for preventing infectious diseases. However, the biggest obstacle to this therapy is delivery of the nucleic acids to the target site, which requires overcoming problems such as capture by the immune system, the need to penetrate biofilms, and degradation of nucleic acid performance. Designing suitable
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Cordyceps militaris: A novel mushroom platform for metabolic engineering Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-19 Jiapeng Zeng, Yue Zhou, Mengdi Lyu, Xinchang Huang, Muyun Xie, Mingtao Huang, Bai-Xiong Chen, Tao Wei
, widely recognized as a medicinal and edible mushroom in East Asia, contains a variety of bioactive compounds, including cordycepin (COR), pentostatin (PTN) and other high-value compounds. This review explores the potential of developing as a cell factory for the production of high-value chemicals and nutrients. This review comprehensively summarizes the fermentation advantages, metabolic networks
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Enzyme-mediated green synthesis of glycosaminoglycans and catalytic process intensification Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-08 Jie Zheng, Xiao-jun Lin, Han Xu, Muhammad Sohail, Liang-an Chen, Xing Zhang
Glycosaminoglycans (GAGs) are a family of structurally complex heteropolysaccharides that play pivotal roles in biological functions, including the regulation of cell proliferation, enzyme inhibition, and activation of growth factor receptors. Therefore, the synthesis of GAGs is a hot research topic in drug development. The enzymatic synthesis of GAGs has received widespread attention due to their
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Advances and opportunities in process analytical technologies for viral vector manufacturing Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-05 Sobhana A. Sripada, Mahshid Hosseini, Srivatsan Ramesh, Junhyeong Wang, Kimberly Ritola, Stefano Menegatti, Michael A. Daniele
Viral vectors are an emerging, exciting class of biologics whose application in vaccines, oncology, and gene therapy has grown exponentially in recent years. Following first regulatory approval, this class of therapeutics has been vigorously pursued to treat monogenic disorders including orphan diseases, entering hundreds of new products into pipelines. Viral vector manufacturing supporting clinical
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Nucleic acid-responsive smart systems for controlled cargo delivery Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-31 Akbar Hasanzadeh, Arefeh Ebadati, Sara Saeedi, Babak Kamali, Hamid Noori, Behnam Jamei, Michael R. Hamblin, Yong Liu, Mahdi Karimi
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The potential of Pavlovophyceae species as a source of valuable carotenoids and polyunsaturated fatty acids for human consumption Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-20 Filipe Maciel, Leandro Madureira, Pedro Geada, José António Teixeira, Joana Silva, António Augusto Vicente
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ATP regeneration by ATPases for in vitro biotransformation Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-17 Lijing Chang, Huijuan Cui, Fei Li, Yi-Heng P. Job Zhang, Lingling Zhang
Adenosine triphosphate (ATP) regeneration is a significant step in both living cells and biotransformation (BT). Rotary motor ATP synthases (ATPases), which regenerate ATP in living cells, have been widely assembled in biomimetic structures for ATP synthesis. In this review, we present a comprehensive overview of ATPases, including the working principle, orientation and distribution density properties
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Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-16 Cristobal Camarena-Bernard, Victor Pozzobon
Increased consumer awareness for healthier and more sustainable products has driven the search for naturally sourced compounds as substitutes for chemically synthesized counterparts. Research on pigments of natural origin, such as carotenoids, particularly lutein, has been increasing for over three decades. Lutein is recognized for its antioxidant and photoprotective activity. Its ability to cross
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Co-utilization of carbon sources in microorganisms for the bioproduction of chemicals Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-15 Qian Ma, Jinhang Yi, Yulin Tang, Zihao Geng, Chunyue Zhang, Wenchao Sun, Zhengkai Liu, Wenwen Xiong, Heyun Wu, Xixian Xie
Carbon source is crucial for the cell growth and metabolism in microorganisms, and its utilization significantly affects the synthesis efficiency of target products in microbial cell factories. Compared with a single carbon source, co-utilizing carbon sources provide an alternative approach to optimize the utilization of different carbon sources for efficient biosynthesis of many chemicals with higher
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A roadmap for model-based bioprocess development Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-15 Khadija Mu'azzam, Francisco Vitor Santos da Silva, Jason Murtagh, Maria Jose Sousa Gallagher
The bioprocessing industry is undergoing a significant transformation in its approach to quality assurance, shifting from the traditional Quality by Testing (QbT) to Quality by Design (QbD). QbD, a systematic approach to quality in process development, integrates quality into process design and control, guided by regulatory frameworks. This paradigm shift enables increased operational efficiencies
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Hydrogen production pathways in Clostridia and their improvement by metabolic engineering Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-14 Roberto Mazzoli, Simone Pescarolo, Giorgio Gilli, Gianfranco Gilardi, Francesca Valetti
Biological production of hydrogen has a tremendous potential as an environmentally sustainable technology to generate a clean fuel. Among the different available methods to produce biohydrogen, dark fermentation features the highest productivity and can be used as a means to dispose of organic waste biomass. Within this approach, Clostridia have the highest theoretical H production yield. Nonetheless
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Unlocking the potential of enzyme engineering via rational computational design strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-11 Lei Zhou, Chunmeng Tao, Xiaolin Shen, Xinxiao Sun, Jia Wang, Qipeng Yuan
Enzymes play a pivotal role in various industries by enabling efficient, eco-friendly, and sustainable chemical processes. However, the low turnover rates and poor substrate selectivity of enzymes limit their large-scale applications. Rational computational enzyme design, facilitated by computational algorithms, offers a more targeted and less labor-intensive approach. There has been notable advancement
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Overview of indigo biosynthesis by Flavin-containing Monooxygenases: History, industrialization challenges, and strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-08 Changxin Fan, Ziqi Xie, Da Zheng, Ruihan Zhang, Yijin Li, Jiacheng Shi, Mingyuan Cheng, Yifei Wang, Yu Zhou, Yi Zhan, Yunjun Yan
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Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-05 Tian-Jie Ao, Chen-Guang Liu, Zhao-Yong Sun, Xin-Qing Zhao, Yue-Qin Tang, Feng-Wu Bai
Anaerobic digestion (AD) is an effective and applicable technology for treating organic wastes to recover bioenergy, but it is limited by various drawbacks, such as long start-up time for establishing a stable process, the toxicity of accumulated volatile fatty acids and ammonia nitrogen to methanogens resulting in extremely low biogas productivities, and a large amount of impurities in biogas for
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Surfactant-mediated bio-manufacture: A unique strategy for promoting microbial biochemicals production Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-03 Yunxin Yi, Xiaofan Jin, Moutong Chen, Teodora Emilia Coldea, Haifeng Zhao
Biochemicals are widely used in the medicine and food industries and are more efficient and safer than synthetic chemicals. The amphipathic surfactants can interact with the microorganisms and embed the extracellular metabolites, which induce microbial metabolites secretion and biosynthesis, performing an attractive prospect of promoting the biochemical production. However, the commonness and differences
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Unearthing naturally-occurring cyclic antibacterial peptides and their structural optimization strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2024-05-03 Ping Zeng, Honglan Wang, Pengfei Zhang, Sharon Shui Yee Leung
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Aptamer-modified paper-based analytical devices for the detection of food hazards: Emerging applications and future perspective Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-30 Mingwei Qin, Imran Mahmood Khan, Ning Ding, Shuo Qi, Xiaoze Dong, Yin Zhang, Zhouping Wang
Food analysis plays a critical role in assessing human health risks and monitoring food quality and safety. Currently, there is a pressing need for a reliable, portable, and quick recognition element for point-of-care testing (POCT) to better serve the demands of on-site food analysis. Aptamer-modified paper-based analytical devices (Apt-PADs) have excellent characteristics of high portability, high
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Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-29 David Catalán-Tatjer, Konstantina Tzimou, Lars K. Nielsen, Jesús Lavado-García
Recombinant adeno-associated viruses (rAAVs) stand at the forefront of gene therapy applications, holding immense significance for their safe and efficient gene delivery capabilities. The constantly increasing and unmet demand for rAAVs underscores the need for a more comprehensive understanding of AAV biology and its impact on rAAV production. In this literature review, we delved into AAV biology
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Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-27 Paola Andrea Palacios, Jo Philips, Anders Bentien, Michael Vedel Wegener Kofoed
Electromethanogenesis has emerged as a biological branch of Power-to-X technologies that implements methanogenic microorganisms, as an alternative to chemical Power-to-X, to convert electrical power from renewable sources, and CO into methane. Unlike biomethanation processes where CO is converted via exogenously added hydrogen, electromethanogenesis occurs in a bioelectrochemical set-up that combines
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Sustainable media feedstocks for cellular agriculture Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-26 Lutz Grossmann
The global food system is shifting towards cellular agriculture, a second domestication marked by cultivating microorganisms and tissues for sustainable food production. This involves tissue engineering, precision fermentation, and microbial biomass fermentation to establish food value chains independent of traditional agriculture. However, these techniques rely on growth media sourced from agricultural
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Carbohydrate binding modules: Compact yet potent accessories in the specific substrate binding and performance evolution of carbohydrate-active enzymes Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-26 Yuxian You, Haocun Kong, Caiming Li, Zhengbiao Gu, Xiaofeng Ban, Zhaofeng Li
Carbohydrate binding modules (CBMs) are independent non-catalytic domains widely found in carbohydrate-active enzymes (CAZymes), and they play an essential role in the substrate binding process of CAZymes by guiding the appended catalytic modules to the target substrates. Owing to their precise recognition and selective affinity for different substrates, CBMs have received increasing research attention
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Engineering the next-generation synthetic cell factory driven by protein engineering Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-23 Ailin Guan, Zixi He, Xin Wang, Zhi-Jun Jia, Jiufu Qin
Synthetic cell factory offers substantial advantages in economically efficient production of biofuels, chemicals, and pharmaceutical compounds. However, to create a high-performance synthetic cell factory, precise regulation of cellular material and energy flux is essential. In this context, protein components including enzymes, transcription factor-based biosensors and transporters play pivotal roles
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Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-22 Merve Atasoy, William T. Scott Jr, Alberte Regueira, Miguel Mauricio-Iglesias, Peter J. Schaap, Hauke Smidt
In recent years, there has been growing interest in harnessing anaerobic digestion technology for resource recovery from waste streams. This approach has evolved beyond its traditional role in energy generation to encompass the production of valuable carboxylic acids, especially volatile fatty acids (VFAs) like acetic acid, propionic acid, and butyric acid. VFAs hold great potential for various industries
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Optimization strategies for CO2 biological fixation Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-19 Xiutao Liu, Linqing Li, Guang Zhao, Peng Xiong
Global sustainable development faces a significant challenge in effectively utilizing CO. Meanwhile, CO biological fixation offers a promising solution. CO has the highest oxidation state (+4 valence state), whereas typical multi‑carbon chemicals have lower valence states. The Gibbs free energy (Δ) changes of CO reductive reactions are generally positive and this renders it necessary to input different
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The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-12 Ruchika, Nabab Khan, Shagun Sanjivv Dogra, Ankit Saneja
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Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-07 Lijuan Wang, Yingying Guo, Yizhou Shen, Kun Yang, Xue Cai, Bo Zhang, Zhiqiang Liu, Yuguo Zheng
L-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with being extensively studied as the preferred strain. This preference is due to its ability
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Recent advances in design and application of synthetic membraneless organelles Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-06 Li Wan, Yingying Zhu, Wenli Zhang, Wanmeng Mu
Membraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) have been extensively studied due to their spatiotemporal control of biochemical and cellular processes in living cells. These findings have provided valuable insights into the physicochemical principles underlying the formation and functionalization of biomolecular condensates, which paves the way for the development of
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Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-06 Zi-Xu Zhang, Ying-Shuang Xu, Zi-Jia Li, Lu-Wei Xu, Wang Ma, Ying-Feng Li, Dong-Sheng Guo, Xiao-Man Sun, He Huang
are marine microorganisms known for their fast growth and ability to store lipids, making them useful for producing polyunsaturated fatty acids (PUFAs), biodiesel, squalene, and carotenoids. However, the high cost of production, mainly due to expensive fermentation components, limits their wider use. A significant challenge in this context is the need to balance production costs with the value of the
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Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-04 Chengcheng Jiang, Yuqi Ma, Wei Wang, Jingjing Sun, Jianhua Hao, Xiangzhao Mao
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Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-04-03 Shi-Peng Zhou, Ya-Ping Xue, Yu-Guo Zheng
Nitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting
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Inteins: A Swiss army knife for synthetic biology Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-27 Stanislav Anastassov, Maurice Filo, Mustafa Khammash
Inteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including
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Expanding the horizon of transient CAR T therapeutics using virus-free technology Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-26 Lucia Enriquez-Rodriguez, Noha Attia, Idoia Gallego, Mohamed Mashal, Iván Maldonado, Gustavo Puras, José Luis Pedraz
The extraordinary success that chimeric antigen receptor (CAR) T cell therapies have shown over the years on fighting hematological malignancies is evidenced by the six FDA-approved products present on the market. CAR T treatments have forever changed the way we understand cellular immunotherapies, as current research in the topic is expanding even outside the field of cancer with very promising results
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The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-26 Jianfei Wang, Jiaqi Huang, Shijie Liu
As an energy-storage substance of microorganisms, polyhydroxybutyrate (PHB) is a promising alternative to petrochemical polymers. Under appropriate fermentation conditions, PHB-producing strains with metabolic diversity can efficiently synthesize PHB using various carbon sources. Carbon-rich wastes may serve as alternatives to pure sugar substrates to reduce the cost of PHB production. Genetic engineering
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Rational construction of synthetic consortia: Key considerations and model-based methods for guiding the development of a novel biosynthesis platform Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-24 Yu Liu, Boyuan Xue, Hao Liu, Shaojie Wang, Haijia Su
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Biocatalysis of CO2 and CH4: Key enzymes and challenges Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-23 Aipeng Li, Xupeng Cao, Rongzhan Fu, Shuqi Guo, Qiang Fei
Mitigating greenhouse gas emissions is a critical challenge for promoting global sustainability. The utilization of CO and CH as substrates for the production of valuable products offers a promising avenue for establishing an eco-friendly economy. Biocatalysis, a sustainable process utilizing enzymes to facilitate biochemical reactions, plays a significant role in upcycling greenhouse gases. This review
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Mobile genetic element-based gene editing and genome engineering: Recent advances and applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-22 Jaeseong Hwang, Dae-Yeol Ye, Gyoo Yeol Jung, Sungho Jang
Genome engineering has revolutionized several scientific fields, ranging from biochemistry and fundamental research to therapeutic uses and crop development. Diverse engineering toolkits have been developed and used to effectively modify the genome sequences of organisms. However, there is a lack of extensive reviews on genome engineering technologies based on mobile genetic elements (MGEs), which
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Harnessing recalcitrant lignocellulosic biomass for enhanced biohydrogen production: Recent advances, challenges, and future perspective Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-21 Sameh S. Ali, Rania Al-Tohamy, Tamer Elsamahy, Jianzhong Sun
Biohydrogen (Bio-H) is widely recognized as a sustainable and environmentally friendly energy source, devoid of any detrimental impact on the environment. Lignocellulosic biomass (LB) is a readily accessible and plentiful source material that can be effectively employed as a cost-effective and sustainable substrate for Bio-H production. Despite the numerous challenges, the ongoing progress in LB pretreatment
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Diverse models of cavity engineering in enzyme modification: Creation, filling, and reshaping Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-20 Zehua Zhang, Yongchao Cai, Nan Zheng, Yu Deng, Ling Gao, Qiong Wang, Xiaole Xia
Most enzyme modification strategies focus on designing the active sites or their surrounding structures. Interestingly, a large portion of the enzymes (60%) feature active sites located within spacious cavities. Despite recent discoveries, cavity-mediated enzyme engineering remains crucial for enhancing enzyme properties and unraveling folding-unfolding mechanisms. Cavity engineering influences enzyme
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Engineering aspects of lipid-based delivery systems: In vivo gene delivery, safety criteria, and translation strategies Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-20 Ismail Eş, Aneesh Thakur, Amin Mousavi Khaneghah, Camilla Foged, Lucimara Gaziola de la Torre
Defects in the genome cause genetic diseases and can be treated with gene therapy. Due to the limitations encountered in gene delivery, lipid-based supramolecular colloidal materials have emerged as promising gene carrier systems. In their non-functionalized form, lipid nanoparticles often demonstrate lower transgene expression efficiency, leading to suboptimal therapeutic outcomes, specifically through
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A domain swapping strategy to create modular transcriptional regulators for novel topology in genetic network Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-19 Clement T.Y. Chan, Vincenzo Kennedy, Sahaj Kinshuk
Transcriptional regulators generate connections between biological signals and genetic outputs. They are used robustly for sensing input signals in building genetic circuits. However, each regulator can only generate a fixed connection, which generates constraints in linking multiple signals for more complex processes. Recent studies discovered that a domain swapping strategy can be applied to various
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Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-18 Minrui Lu, Yuanyuan Sha, Vinod Kumar, Zhaoxian Xu, Rui Zhai, Mingjie Jin
As a sustainable and renewable alternative to petroleum fuels, advanced biofuels shoulder the responsibility of energy saving, emission reduction and environmental protection. Traditional engineering of cell factories for production of advanced biofuels lacks efficient high-throughput screening tools and regulating systems, impeding the improvement of cellular productivity and yield. Transcription
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Multifaced application of AFEX-pretreated biomass in producing second-generation biofuels, ruminant animal feed, and value-added bioproducts Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-16 Maedeh Mohammadi, Mahsa Alian, Bruce Dale, Bryan Ubanwa, Venkatesh Balan
Lignocellulosic biomass holds a crucial position in the prospective bio-based economy, serving as a sustainable and renewable source for a variety of bio-based products. These products play a vital role in displacing fossil fuels and contributing to environmental well-being. However, the inherent recalcitrance of biomass poses a significant obstacle to the efficient access of sugar polymers. Consequently
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Recent advances in phyto-combined remediation of heavy metal pollution in soil Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-07 Shaoxiong Deng, Xuan Zhang, Yonghua Zhu, Rui Zhuo
The global industrialization and modernization have witnessed a rapid progress made in agricultural production, along with the issue of soil heavy metal (HM) pollution, which has posed severe threats to soil quality, crop yield, and human health. Phytoremediation, as an alternative to physical and chemical methods, offers a more cost-effective, eco-friendly, and aesthetically appealing means for in-situ
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Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-07 Chengmei Yin, Xiangyao Chen, Haiyang Zhang, Yong Xue, Hao Dong, Xiangzhao Mao
Non-homogeneous enzyme-catalyzed systems are more widely used than homogeneous systems. Distinguished from the conventional biphasic approach, Pickering emulsion stabilized by ultrafine solid particles opens up an innovative platform for biocatalysis. Their vast specific surface area significantly enhances enzyme-substrate interactions, dramatically increasing catalytic efficiency. This review comprehensively
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Next generation probiotics: Engineering live biotherapeutics Biotechnol. Adv. (IF 12.1) Pub Date : 2024-03-02 Sanjeeva Kumar Murali, Thomas J. Mansell
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The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-27 Jianlong Wang, Xuan Guo
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New opportunities and perspectives on biosynthesis and bioactivities of secondary metabolites from Aloe vera Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-21 Mrudulakumari Vasudevan Ushasree, Qi Jia, Seon Gil Do, Eun Yeol Lee
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Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-19 Xiaoxiao Li, Zhengyu Jin, Yuxiang Bai, Birte Svensson
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Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-13 Brahma N. Singh, Meisam Tabatabaei, Abhay Pandit, Lothar Elling, Vijai Kumar Gupta
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CRISPR-Cas systems of lactic acid bacteria and applications in food science Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-11 Yanhua Cui, Xiaojun Qu
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR associated proteins) systems are widely distributed in lactic acid bacteria (LAB), contributing to their RNA-mediated adaptive defense immunity. The CRISPR-Cas-based genetic tools have exhibited powerful capability. It has been highly utilized in different organisms, accelerating the development of life science. The review
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The thin line between monooxygenases and peroxygenases. P450s, UPOs, MMOs, and LPMOs: A brick to bridge fields of expertise Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-08 Davide Decembrino, David Cannella
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Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing Biotechnol. Adv. (IF 12.1) Pub Date : 2024-02-07 Yongdan Wang, Qiang Fu, So Young Park, Yong Suk Lee, Seo-Young Park, Dong-Yup Lee, Seongkyu Yoon
Recombinant adeno-associated virus (rAAV) is one of the prominent gene delivery vehicles that has opened promising opportunities for novel gene therapeutic approaches. However, the current major viral vector production platform, triple transfection in mammalian cells, may not meet the increasing demand. Thus, it is highly required to understand production bottlenecks from the host cell perspective
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Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects Biotechnol. Adv. (IF 12.1) Pub Date : 2024-01-26 Zhijin Gong, Jiayao Chen, Xinyu Jiao, Hao Gong, Danzi Pan, Lingli Liu, Yang Zhang, Tianwei Tan
The construction of high-performance microbial cell factories (MCFs) is the centerpiece of biomanufacturing. However, the complex metabolic regulatory network of microorganisms poses great challenges for the efficient design and construction of MCFs. The genome-scale metabolic network models (GSMs) can systematically simulate the metabolic regulation process of microorganisms , providing effective
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Molecular imprinting technology for biomedical applications Biotechnol. Adv. (IF 12.1) Pub Date : 2024-01-22 Julija Sarvutienė, Urte Samukaite-Bubniene, Simonas Ramanavicius, Arunas Ramanavicius
Molecularly imprinted polymers (MIPs), a type of biomimetic material, have attracted considerable interest owing to their cost-effectiveness, good physiochemical stability, favourable specificity and selectivity for target analytes, and widely used for various biological applications. It was demonstrated that MIPs with significant selectivity towards protein-based targets could be applied in medicine
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PHA is not just a bioplastic! Biotechnol. Adv. (IF 12.1) Pub Date : 2024-01-23 Helen Park, Hongtao He, Xu Yan, Xu Liu, Nigel S. Scrutton, Guo-Qiang Chen
Polyhydroxyalkanoates (PHA) have evolved into versatile biopolymers, transcending their origins as mere bioplastics. This extensive review delves into the multifaceted landscape of PHA applications, shedding light on the diverse industries that have harnessed their potential. PHA has proven to be an invaluable eco-conscious option for packaging materials, finding use in films foams, paper coatings
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Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review Biotechnol. Adv. (IF 12.1) Pub Date : 2024-01-09 Jinsong Liang, Ru Zhang, Jianning Chang, Le Chen, Mohammad Nabi, Haibo Zhang, Guangming Zhang, Panyue Zhang
The rumen of ruminants is a natural anaerobic fermentation system that efficiently degrades lignocellulosic biomass and mainly depends on synergistic interactions between multiple microbes and their secreted enzymes. Ruminal microbes have been employed as biomass waste converters and are receiving increasing attention because of their degradation performance. To explore the application of ruminal microbes
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