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Structural insights into the assembly pathway of the Helicobacter pylori CagT4SS outer membrane core complex Structure (IF 4.4) Pub Date : 2024-07-19
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Intracellular tau fragment droplets serve as seeds for tau fibrils Structure (IF 4.4) Pub Date : 2024-07-19
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Structural insights into an atypical histone binding mechanism by a PHD finger Structure (IF 4.4) Pub Date : 2024-07-18
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Cryo-EM structures reveal the molecular mechanism of HflX-mediated erythromycin resistance in mycobacteria Structure (IF 4.4) Pub Date : 2024-07-18
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Crystal structure of lipase from Pseudomonas aeruginosa reveals an unusual catalytic triad conformation Structure (IF 4.4) Pub Date : 2024-07-17
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They all rock: A systematic comparison of conformational movements in LeuT-fold transporters Structure (IF 4.4) Pub Date : 2024-07-17
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Berberine analog of chloramphenicol exhibits a distinct mode of action and unveils ribosome plasticity Structure (IF 4.4) Pub Date : 2024-07-16
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Structural basis of homodimerization of the JNK scaffold protein JIP2 and its heterodimerization with JIP1 Structure (IF 4.4) Pub Date : 2024-07-15
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Cryo-EM structure of the R388 plasmid conjugative pilus reveals a helical polymer characterized by an unusual pilin/phospholipid binary complex Structure (IF 4.4) Pub Date : 2024-07-12 Abhinav K. Vadakkepat, Songlin Xue, Adam Redzej, Terry K. Smith, Brian T. Ho, Gabriel Waksman
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Pho pictures provide powerful perspectives of phosphate importing proteins Structure (IF 4.4) Pub Date : 2024-07-11 David Speedman, David B. Sauer
In this issue of Structure, Schneider et al.1 report multiple structures of the low-affinity inorganic-phosphate transporter Pho90 from Saccharomyces cerevisiae. With remarkable resolution of the Divalent Anion Sodium Symporter family member, their cryo-EM studies of this fungal protein reveal new modes of sodium, substrate, and lipid binding.
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The merger of two major families of proteins that regulate cellular processes Structure (IF 4.4) Pub Date : 2024-07-11 Karlett J. Parra
In this issue of Structure, Oot and Wilkens1 present new mechanistic insights to finally merge the function of V-ATPase and TLDc domain proteins. They show that TLDc proteins directly affect V-ATPase activity and assembly, expanding our understanding of how V-ATPase and TLDc proteins exert a plethora of biological functions.
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Squishy to crusty: Biophysics reveal the molecular details of FUS droplet maturation Structure (IF 4.4) Pub Date : 2024-07-11 Erich J. Sohn, David S. Libich
In a recent issue of Nature Chemical Biology, Emmanouilidis et al. (2024) investigate the maturation of biomolecular condensates of FUS1-267 and probe the molecular details of droplet aging. They observe that the liquid-to-solid transition of the droplet is mediated at the surface by FUS1–267 molecules that have adopted β-strand conformations.
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Binding of steroid substrates reveals the key to the productive transition of the cytochrome P450 OleP Structure (IF 4.4) Pub Date : 2024-07-05 Antonella Costanzo, Francesca Fata, Ida Freda, Maria Laura De Sciscio, Elena Gugole, Giovanni Bulfaro, Matteo Di Renzo, Luca Barbizzi, Cécile Exertier, Giacomo Parisi, Marco D’Abramo, Beatrice Vallone, Carmelinda Savino, Linda Celeste Montemiglio
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Molecular mechanism of contactin 2 homophilic interaction Structure (IF 4.4) Pub Date : 2024-07-04 Shanghua Fan, Jianfang Liu, Nicolas Chofflet, Aaron O. Bailey, William K. Russell, Ziqi Zhang, Hideto Takahashi, Gang Ren, Gabby Rudenko
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Molecular insights into the initiation step of the Rcs signaling pathway Structure (IF 4.4) Pub Date : 2024-07-03 Nobuhiko Watanabe, Alexei Savchenko
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Mechanism of ceramide synthase inhibition by fumonisin B1 Structure (IF 4.4) Pub Date : 2024-07-03 Zike Zhang, Qi Fang, Tian Xie, Xin Gong
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Major-groove sequence-specific RNA recognition by LoaP, a paralog of transcription elongation factor NusG Structure (IF 4.4) Pub Date : 2024-07-02 Amr Elghondakly, Madison D. Jermain, Wade C. Winkler, Adrian R. Ferré-D’Amaré
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Markerauto2: A fast and robust fully automatic fiducial marker-based tilt series alignment software for electron tomography Structure (IF 4.4) Pub Date : 2024-06-26 Zihe Xu, Hongjia Li, Xiaohua Wan, Jose-Jesus Fernández, Fei Sun, Fa Zhang, Renmin Han
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De novo design of SARS-CoV-2 main protease inhibitors with characteristic binding modes Structure (IF 4.4) Pub Date : 2024-06-25 Yan Zhu, Jiaolong Meng, Bo Feng, Yao Zhao, Yi Zang, Lingling Lu, Mingbo Su, Qi Yang, Qi Zhang, Lu Feng, Jinyi Zhao, Maolin Shao, Yuanyuan Ma, Xiuna Yang, Haitao Yang, Jia Li, Xuefeng Jiang, Zihe Rao
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Structural insights into the role and targeting of EGFRvIII Structure (IF 4.4) Pub Date : 2024-06-21 Atrish Bagchi, Steven E. Stayrook, Katerina T. Xenaki, Chrystal A. Starbird, Sofia Doulkeridou, Rachid el Khoulati, Rob C. Roovers, Karl R. Schmitz, Paul M.P. van Bergen en Henegouwen, Kathryn M. Ferguson
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Structural investigation of the docking domain assembly from trans-AT polyketide synthases Structure (IF 4.4) Pub Date : 2024-06-21 Se-Young Son, Da-Woon Bae, Eunji Kim, Bo-Gyeong Jeong, Myeong-Yeon Kim, So-Yeon Youn, Soojung Yi, Gyeongmin Kim, Ji-Sook Hahn, Nam Ki Lee, Yeo Joon Yoon, Sun-Shin Cha
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Roles of the gate loop in β-arrestin-1 conformational dynamics and phosphorylated receptor interaction Structure (IF 4.4) Pub Date : 2024-06-17 Kiae Kim, Janbolat Ashim, Donghee Ham, Wookyung Yu, Ka Young Chung
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Structure and bioactivity of an insecticidal trans-defensin from assassin bug venom Structure (IF 4.4) Pub Date : 2024-06-17 Andrew A. Walker, Yanni K.-Y. Chin, Shaodong Guo, Jiayi Jin, Evienne Wilbrink, Mohaddeseh Hedayati Goudarzi, Hayden Wirth, Eric Gordon, Christiane Weirauch, Glenn F. King
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Real-time structural characterization of protein response to a caged compound by fast detector readout and high-brilliance synchrotron radiation Structure (IF 4.4) Pub Date : 2024-06-17 Konstantinos Magkakis, Fredrik Orädd, Byungnam Ahn, Vanessa Da Silva, Roberto Appio, Tomás S. Plivelic, Magnus Andersson
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Protein engineering enables a soakable crystal form of human CDK7 primed for high-throughput crystallography and structure-based drug design Structure (IF 4.4) Pub Date : 2024-06-12 Manjeet Mukherjee, Philip J. Day, Duncan Laverty, Juan A. Bueren-Calabuig, Andrew J. Woodhead, Charlotte Griffiths-Jones, Steve Hiscock, Charlotte East, Susan Boyd, Marc O'Reilly
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Mechanism of heterochromatin remodeling revealed by the DDM1 bound nucleosome structures Structure (IF 4.4) Pub Date : 2024-06-12 Hongwei Zhang, Zhanxi Gu, Yuan Zeng, Yu Zhang
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Biochemical and structural characterization of the DNA-binding properties of human TRIP4 ASCH domain reveals insights into its functional role Structure (IF 4.4) Pub Date : 2024-06-12 Chengtao Hu, Ziyue Chen, Guanchao Wang, Hui Yang, Jianping Ding
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An expanded trove of fragment-bound structures for the allosteric enzyme PTP1B from computational reanalysis of large-scale crystallographic data Structure (IF 4.4) Pub Date : 2024-06-10 Tamar Mehlman, Helen M. Ginn, Daniel A. Keedy
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Fraternal twins at work: Structures of PLD3/4 reveal mechanism for lysosomal nucleic acid breakdown Structure (IF 4.4) Pub Date : 2024-06-06 Oliver Daumke, Markus Damme
Phospholipase D (PLD) family proteins degrade phospholipids and nucleic acids. In the current issue of Structure, Yuan et al.1 report crystal structures of lysosomal PLD3 and PLD4 with and without a single-stranded DNA substrate. Their manuscript reveals a catalytic ping-pong mechanism and explains how disease-associated mutations compromise PLD3/4 function.
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Trading in catalytic power for sensing Structure (IF 4.4) Pub Date : 2024-06-06 Zhoufei Hei, Jing Wang, Pengfei Fang
In this issue of Structure, Yin et al.1 present the CryoEM structure of the HisRS-like domain of human GCN2 and demonstrate that it is a pseudoenzyme, which binds uncharged tRNA in a different manner than HisRS and does not bind histidine and ATP.
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Watching atomically resolved structural dynamics—Blinded by the light Structure (IF 4.4) Pub Date : 2024-06-06 Karsten Heyne, Stefan Haacke, R.J. Dwayne Miller
In a recent issue of Nature, Barends et al.1 studied the photodissociation of carboxymyoglobin with ultrafast laser pump-probe serial femtosecond crystallography experiments. They observed significant differences in heme protein structural dynamics for biologically relevant 1-photon excitation relative to high excitation leading to the absorption of several photons per heme.
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Inside the capsid: Revealing viral genome organization through multiscale simulations Structure (IF 4.4) Pub Date : 2024-06-06 Eric R. May
In a recent issue of Nature, Coshic et al. employ a computational multiscale approach to package the complete HK97 viral genome into its capsid. They find both good agreement with experimental observations and shed new light on the heterogeneity of genome structures and the mechanism by which they package.
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Cryo-electron tomography reveals the packaging pattern of RuBisCOs in Synechococcus β-carboxysome Structure (IF 4.4) Pub Date : 2024-05-31 Wen-Wen Kong, Yun Zhu, Heng-Rui Zhao, Kang Du, Rui-Qian Zhou, Bo Li, Feng Yang, Pu Hou, Xia-He Huang, Yuxing Chen, Ying-Chun Wang, Fei Sun, Yong-Liang Jiang, Cong-Zhao Zhou
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Tilt-series-based joint CTF estimation for cryo-electron tomography Structure (IF 4.4) Pub Date : 2024-05-31 Ranhao Zhang, Yuan Shen, Xueming Li
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Structural insights into the Langya virus attachment glycoprotein Structure (IF 4.4) Pub Date : 2024-05-29 Chenghai Wang, Min Li, Yufan Wang, Qiang Ding, Shilong Fan, Jun Lan
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Structural basis for inhibition of the lysosomal two-pore channel TPC2 by a small molecule antagonist Structure (IF 4.4) Pub Date : 2024-05-29 Gamma Chi, Dawid Jaślan, Veronika Kudrina, Julia Böck, Huanyu Li, Ashley C.W. Pike, Susanne Rautenberg, Einar Krogsaeter, Tina Bohstedt, Dong Wang, Gavin McKinley, Alejandra Fernandez-Cid, Shubhashish M.M. Mukhopadhyay, Nicola A. Burgess-Brown, Marco Keller, Franz Bracher, Christian Grimm, Katharina L. Dürr
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Affinity-directed substrate/H+-antiport by a MATE transporter Structure (IF 4.4) Pub Date : 2024-05-29 Koh Takeuchi, Takumi Ueda, Misaki Imai, Miwa Fujisaki, Mie Shimura, Yuji Tokunaga, Yutaka Kofuku, Ichio Shimada
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Structural basis for the broad antigenicity of the computationally optimized influenza hemagglutinin X6 Structure (IF 4.4) Pub Date : 2024-05-28 Kaito A. Nagashima, John V. Dzimianski, Meng Yang, Jan Abendroth, Giuseppe A. Sautto, Ted M. Ross, Rebecca M. DuBois, Thomas E. Edwards, Jarrod J. Mousa
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Structural and functional insights in flavivirus NS5 proteins gained by the structure of Ntaya virus polymerase and methyltransferase Structure (IF 4.4) Pub Date : 2024-05-22 Kateřina Krejčová, Petra Krafcikova, Martin Klima, Dominika Chalupska, Karel Chalupsky, Eva Zilecka, Evzen Boura
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Bilayer lipids modulate ligand binding to atypical chemokine receptor 3 Structure (IF 4.4) Pub Date : 2024-05-21 Stefanie Alexandra Eberle, Martin Gustavsson
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DomainFit: Identification of protein domains in cryo-EM maps at intermediate resolution using AlphaFold2-predicted models Structure (IF 4.4) Pub Date : 2024-05-15 Jerry Gao, Maxwell Tong, Chinkyu Lee, Jacek Gaertig, Thibault Legal, Khanh Huy Bui
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Structural characterization of Thogoto Virus nucleoprotein provides insights into viral RNA encapsidation and RNP assembly Structure (IF 4.4) Pub Date : 2024-05-14 Alexej Dick, Vasilii Mikirtumov, Jonas Fuchs, Ferdinand Krupp, Daniel Olal, Elias Bendl, Thiemo Sprink, Christoph Diebolder, Mikhail Kudryashev, Georg Kochs, Yvette Roske, Oliver Daumke
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Cobalamin decyanation by the membrane transporter BtuM Structure (IF 4.4) Pub Date : 2024-05-10 Jose M. Martínez Felices, Yan Borges Barreto, Chancievan Thangaratnarajah, Jacob J. Whittaker, Adriano M. Alencar, Albert Guskov, Dirk J. Slotboom
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Polymorphic residues in HLA-B that mediate HIV control distinctly modulate peptide interactions with both TCR and KIR molecules Structure (IF 4.4) Pub Date : 2024-05-10 Rhoda Tano-Menka, Nishant K. Singh, Itai Muzhingi, Xiaolong Li, Michael V. Mandanas, Clarety Kaseke, Charles R. Crain, Angela Zhang, Funsho J. Ogunshola, Liza Vecchiarello, Alicja Piechocka-Trocha, Arman Bashirova, Michael E. Birnbaum, Mary Carrington, Bruce D. Walker, Gaurav D. Gaiha
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αMI-domain of integrin Mac-1 binds the cytokine pleiotrophin using multiple mechanisms Structure (IF 4.4) Pub Date : 2024-05-09 Hoa Nguyen, Nataly P. Podolnikova, Tatiana P. Ugarova, Xu Wang
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Slower CDK4 and faster CDK2 activation in the cell cycle Structure (IF 4.4) Pub Date : 2024-05-03 Wengang Zhang, Yonglan Liu, Hyunbum Jang, Ruth Nussinov
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Structural elucidation of the mesothelin-mucin-16/CA125 interaction Structure (IF 4.4) Pub Date : 2024-05-03
Mesothelin (MSLN) is a cell-surface glycoprotein expressed at low levels on normal mesothelium but overexpressed in many cancers. Mesothelin has been …
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DR-BERT: A protein language model to annotate disordered regions Structure (IF 4.4) Pub Date : 2024-05-02 Ananthan Nambiar, John Malcolm Forsyth, Simon Liu, Sergei Maslov
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Structural and biochemical characterization of a nucleotide hydrolase from Streptococcus pneumonia Structure (IF 4.4) Pub Date : 2024-05-02 Yuping Jin, Jiyuan Ke, Peiyi Zheng, Haiyan Zhang, Zhongliang Zhu, Liwen Niu
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Introducing: Ulrich J. Lorenz, Radoslav I. Enchev, Clare F. Megarity, Helen Ginn, Patrick Rabe, Alice R. Walker, and Kartik Ayyer Structure (IF 4.4) Pub Date : 2024-05-02
In this Voices article we introduce seven impressive young group leaders who spoke at the recent Ringberg Workshop on Structural Biology organized by …
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The critical importance of conditions: Reconciling GPCR functionality and biophysical findings Structure (IF 4.4) Pub Date : 2024-05-02 Melissa A. Reith, Jan K. Rainey
G-protein-coupled receptor (GPCR) activation relies on conformational sampling, a nuanced but functionally key behavior well suited to elucidation by nuclear magnetic resonance (NMR) spectroscopy. In this issue of Structure, Thakur et al.1 demonstrate that judicious choice of experimental conditions for 19F NMR studies of a GPCR enables rationalization of functional and pharmacological behavior, leading
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Enzyme engineering lets us play with new building blocks in non-ribosomal peptide synthesis Structure (IF 4.4) Pub Date : 2024-05-02 Minuri S. Ratnayake, Mathias H. Hansen, Max J. Cryle
In a recent issue of Nature Chemical Biology, Folger et al. demonstrated a high-throughput approach for engineering peptide bond forming domains from non-ribosomal peptide synthesis. A non-ribosomal peptide synthetase module from surfactin biosynthesis was reprogrammed to accept a fatty acid substrate into peptide biosynthesis, thus illustrating the potential of this approach for generating novel bioactive
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Structural insights into the bifunctional enzyme human FAD synthase Structure (IF 4.4) Pub Date : 2024-04-29 Giulia Leo, Piero Leone, Elham Ataie Kachoie, Maria Tolomeo, Michele Galluccio, Cesare Indiveri, Maria Barile, Stefano Capaldi
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Complementary structures of the yeast phosphate transporter Pho90 provide insights into its transport mechanism Structure (IF 4.4) Pub Date : 2024-04-29 Simon Schneider, Werner Kühlbrandt, Özkan Yildiz
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Constitutive activity of ionotropic glutamate receptors via hydrophobic substitutions in the ligand-binding domain Structure (IF 4.4) Pub Date : 2024-04-26 Sandra Seljeset, Oksana Sintsova, Yuhong Wang, Hassan Y. Harb, Timothy Lynagh
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Structural and functional insights into yeast Tbf1 as an atypical telomeric repeat-binding factor Structure (IF 4.4) Pub Date : 2024-04-26 Zhenfang Wu, Xin Gu, Lin Zha, Qingqiu Yang, Yuanze Zhou, Zhixiong Zeng
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Structural and functional characterization of itaconyl-CoA hydratase and citramalyl-CoA lyase involved in itaconate metabolism of Pseudomonas aeruginosa Structure (IF 4.4) Pub Date : 2024-04-26 Qin Huang, Chenxi Duan, Haichuan Ma, Cheng Nong, Qianqian Zheng, Jun Zhou, Ninglin Zhao, Xingyu Mou, Tonggen Liu, Shuang Zou, Ningchuan Yang, Aiping Tong, Wenming Qin, Rui Bao
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OPUS-Rota5: A highly accurate protein side-chain modeling method with 3D-Unet and RotaFormer Structure (IF 4.4) Pub Date : 2024-04-23 Gang Xu, Zhenwei Luo, Yaming Yan, Qinghua Wang, Jianpeng Ma