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Next-generation patient models for colorectal cancer research Nat. Biotechnol. (IF 33.1) Pub Date : 2024-07-02
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High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits Nat. Biotechnol. (IF 33.1) Pub Date : 2024-07-02 Ayano C. Kohlgruber, Mohammad H. Dezfulian, Brandon M. Sie, Charlotte I. Wang, Tomasz Kula, Uri Laserson, H. Benjamin Larman, Stephen J. Elledge
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Tracking-seq reveals the heterogeneity of off-target effects in CRISPR–Cas9-mediated genome editing Nat. Biotechnol. (IF 33.1) Pub Date : 2024-07-02 Ming Zhu, Runda Xu, Junsong Yuan, Jiacheng Wang, Xiaoyu Ren, Tingting Cong, Yaxian You, Anji Ju, Longchen Xu, Huimin Wang, Peiyuan Zheng, Huiying Tao, Chunhua Lin, Honghao Yu, Juanjuan Du, Xin Lin, Wei Xie, Yinqing Li, Xun Lan
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Patient-derived mini-colons enable long-term modeling of tumor–microenvironment complexity Nat. Biotechnol. (IF 33.1) Pub Date : 2024-07-02 L. Francisco Lorenzo-Martín, Nicolas Broguiere, Jakob Langer, Lucie Tillard, Mikhail Nikolaev, George Coukos, Krisztian Homicsko, Matthias P. Lutolf
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Cell–drug conjugates Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-07-01 Yanfang Wang, Jiaqi Shi, Minhang Xin, Anna R. Kahkoska, Jinqiang Wang, Zhen Gu
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Temperature‐responsive hydrogel‐grafted vessel‐on‐a‐chip: Exploring cold‐induced endothelial injury Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-07-01 Chong Shen, Jiajie Li, Wenqi She, Aiping Liu, Qin Meng
Cold‐induced vasoconstriction is a significant contributor that leads to chilblains and hypothermia in humans. However, current animal models have limitations in replicating cold‐induced acral injury due to their low sensitivity to cold. Moreover, existing in vitro vascular chips composed of endothelial cells and perfusion systems lack temperature responsiveness, failing to simulate the vasoconstriction
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3D imaging and analysis to unveil the impact of microparticles on the pellet morphology of filamentous fungi Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-29 Anna Dinius, Henri Müller, Diana Kellhammer, Charlotte Deffur, Stefan Schmideder, Jörg U. Hammel, Rainer Krull, Heiko Briesen
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Reinforcement learning based temperature control of a fermentation bioreactor for ethanol production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-28 Nagabhushanamgari Rajasekhar, Thota Karunakaran Radhakrishnan, Samsudeen Naina Mohamed
Ethanol production is a significant industrial bioprocess for energy. The primary objective of this study is to control the process reactor temperature to get the desired product, that is, ethanol. Advanced model‐based control systems face challenges due to model‐process mismatch, but Reinforcement Learning (RL) is a class of machine learning which can help by allowing agents to learn policies directly
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Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-27 Jiwei Mao, Hongyu Zhang, Yu Chen, Liang Wei, Jun Liu, Jens Nielsen, Yun Chen, Ning Xu
Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic
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Adaptive laboratory evolution in a novel parallel shaken pH‐auxostat Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-27 Burak Sarikaya, Hendrik Bück, Gino Pohen, Filipe Rodrigues, Karsten Günster, Katrin Wefelmeier, Katharina Miebach, Lars M. Blank, Jochen Büchs
Adaptive laboratory evolution (ALE) is a widely used microbial strain development and optimization method. ALE experiments, to select for faster‐growing strains, are commonly performed as serial batch cultivations in shake flasks, serum bottles, or microtiter plates or as continuous cultivations in bioreactors on a laboratory scale. To combine the advantages of higher throughput in parallel shaken
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CRISPR screens of tumor-infiltrating NK cells identify genetic checkpoints for CAR-NK therapy Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-25
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Tailoring silk fibroin fibrous architecture by a high‐yield electrospinning method for fast wound healing possibilities Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-26 Jia‐Chen Zhu, Hui Wang, Chen‐Xing Wu, Ke‐Qin Zhang, Hua Ye
In this study, a novel array electrospinning collector was devised to generate two distinct regenerated silk fibroin (SF) fibrous membranes: ordered and disordered. Leveraging electrostatic forces during the electrospinning process allowed precise control over the orientation of SF fiber, resulting in the creation of membranes comprising both aligned and randomly arranged fiber layers. This innovative
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Increasing access to biotech products for animal agriculture in Sub-Saharan Africa through partnerships Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-25 José de la Fuente, Christian Gortázar, Marinela Contreras, Frederick Kabi, Paul Kasaija, Swidiq Mugerwa, Justus Rutaisire
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In vivo AAV–SB-CRISPR screens of tumor-infiltrating primary NK cells identify genetic checkpoints of CAR-NK therapy Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-25 Lei Peng, Paul A. Renauer, Giacomo Sferruzza, Luojia Yang, Yongji Zou, Zhenghao Fang, Jonathan J. Park, Ryan D. Chow, Yueqi Zhang, Qianqian Lin, Meizhu Bai, Angelica Sanchez, Yongzhan Zhang, Stanley Z. Lam, Lupeng Ye, Sidi Chen
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Chinese hamster ovary cell line engineering strategies for modular production of custom extracellular vesicles Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-25 Braulio Carrillo Sanchez, Matthew Hinchliffe, Mark Ellis, Catherine Simpson, David Humphreys, Bernie Sweeney, Daniel G. Bracewell
Continuously secreted by all cell types, extracellular vesicles (EVs) are small membrane‐bound structures which shuttle bioactive cargo between cells across their external environment. Their central role as natural molecular messengers and ability to cross biological barriers has garnered significant attention in the use of EVs as therapeutic delivery vehicles. Still, harnessing the potential of EVs
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Improvement of TaC9‐ABE mediated correction of human SMN2 gene Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-25 Xiaohua Peng, Yue Chi, Jinling Wang, Shuangpeng Li, Yang Liu, Chengcheng Tang, Xiaoqing Zhou, Xuan Lu, Yue Gao, Liangxue Lai, Min Chen, Qingjian Zou
Spinal muscular atrophy (SMA) is a devastating neuromuscular disease caused by mutations in the survival motor neuron 1 (SMN1) gene. Gene editing technology repairs the conversion of the 6th base T to C in exon 7 of the paralogous SMN2 gene, compensating for the SMN protein expression and promoting the survival and function of motor neurons. However, low editing efficiency and unintended off‐target
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Strategies for the development of metalloimmunotherapies Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-06-24 Xiaoqi Sun, Xingwu Zhou, Xiaoyue Shi, Omar A. Abed, Xinran An, Yu Leo Lei, James J. Moon
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Advancing microbial production through artificial intelligence-aided biology Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-24 Xinyu Gong, Jianli Zhang, Qi Gan, Yuxi Teng, Jixin Hou, Yanjun Lyu, Zhengliang Liu, Zihao Wu, Runpeng Dai, Yusong Zou, Xianqiao Wang, Dajiang Zhu, Hongtu Zhu, Tianming Liu, Yajun Yan
Microbial cell factories (MCFs) have been leveraged to construct sustainable platforms for value-added compound production. To optimize metabolism and reach optimal productivity, synthetic biology has developed various genetic devices to engineer microbial systems by gene editing, high-throughput protein engineering, and dynamic regulation. However, current synthetic biology methodologies still rely
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Metabolomic characterization of monoclonal antibody‐producing Chinese hamster lung (CHL)‐YN cells in glucose‐controlled serum‐free fed‐batch operation Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Puriwat Sukwattananipaat, Hirotaka Kuroda, Noriko Yamano‐Adachi, Takeshi Omasa
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Development of a model for the ethanol concentration limit as a function of temperature and initial substrate concentration using the yeast Saccharomyces cerevisiae Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Ruan Dionízio SILVA, Flávio Luiz Honorato da Silva, Sharline Florentino de Melo Santos, Alberto Brandão Torres Neto, Julice Dutra Lopes, Josilene de Assis Cavalcante
In this study, a model was developed to simulate the effect of temperature () and initial substrate concentration () on the ethanol concentration limit () using the yeast Saccharomyces cerevisiae. To achieve this, regressions were performed using data provided by other authors for to establish a model dependent on and capable of predicting results with statistical significance. After constructing the
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Evaluation of bacteriophages for the alleviation of potential bacterial contamination risks in developmental engineering Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Yu Xiang, Xujin Bao, Tao Sun
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An industrial perspective on metabolic responses of Penicillium chrysogenum to periodic dissolved oxygen feast‐famine cycles in a scale‐down system Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Xueting Wang, Qi Yang, Cees Haringa, Zejian Wang, Ju Chu, Yingping Zhuang, Guan Wang
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Synergetic light and cytokinin treatments mitigate the recombinant protein yield depression induced by high‐density cultivation of hydroponically‐grown Nicotiana benthamiana Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Stefano Bilotta, Gilbert Éthier, Ann‐Catherine Laliberté, Marie‐Claire Goulet, Michèle Martel, Dominique Michaud, Steeve Pepin
Plant molecular farming is currently operating a transition from soil‐based cultures toward hydroponic systems. In this study, we designed a whole‐plant NFT (nutrient film technique) platform for the transient expression of influenza virus‐like particles harboring hemagglutinin H1 proteins in Nicotiana benthamiana. In particular, we examined the effects of plant density during the post‐infiltration
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Machine learning prediction of prime editing efficiency across diverse chromatin contexts Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-21 Nicolas Mathis, Ahmed Allam, András Tálas, Lucas Kissling, Elena Benvenuto, Lukas Schmidheini, Ruben Schep, Tanav Damodharan, Zsolt Balázs, Sharan Janjuha, Eleonora I. Ioannidi, Desirée Böck, Bas van Steensel, Michael Krauthammer, Gerald Schwank
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Functional roles and engineering strategies to improve the industrial functionalities of lactic acid bacteria during food fermentation Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-21 Huan Yang, Liying Hao, Yao Jin, Jun Huang, Rongqing Zhou, Chongde Wu
In order to improve the flavor profiles, food security, probiotic effects and shorten the fermentation period of traditional fermented foods, lactic acid bacteria (LAB) were often considered as the ideal candidate to participate in the fermentation process. In general, LAB strains possessed the ability to develop flavor compounds via carbohydrate metabolism, protein hydrolysis and amino acid metabolism
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Effects of the first successful lawsuit against a consumer neurotechnology company for violating brain data privacy Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-20 José M. Muñoz, José Ángel Marinaro, José Antonio Iglesias, Moisés Sánchez, Natalia Monti, Ciro Colombara, Guido Girardi
On 9 August 2023 the Supreme Court of Chile (SCC) ruled in favor of former senator Guido Girardi in a constitutional protection appeal filed against the neurotechnology company Emotiv1 for violating his rights to privacy and psychological integrity, which are enshrined in Article 19 of Chile’s constitution2. The authors of the present piece — the plaintiff, some of his lawyers and legal advisers, and
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A miniaturized mesoscope for the large-scale single-neuron-resolved imaging of neuronal activity in freely behaving mice Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-06-20 Yuanlong Zhang, Lekang Yuan, Qiyu Zhu, Jiamin Wu, Tobias Nöbauer, Rujin Zhang, Guihua Xiao, Mingrui Wang, Hao Xie, Zengcai Guo, Qionghai Dai, Alipasha Vaziri
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Editor’s pick: Tenpoint Therapeutics Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-19
Each year, Nature Biotechnology highlights companies that have received sizeable early-stage funding in the previous year. Tenpoint Therapeutics optimizes delivery and generation of stem-cell-derived retinal cells for vision repair.
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Tracking gene order provides a new perspective on intraspecific evolution in microbiotas Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-19
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Editor’s pick: Tome Biosciences Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-19
Each year, Nature Biotechnology highlights companies that have received sizeable early-stage funding in the previous year. Tome Biosciences inserts large DNA sequences into precise genomic locations, overcoming limitations of base and prime editing.
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Strain tracking in complex microbiomes using synteny analysis reveals per-species modes of evolution Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-19 Hagay Enav, Inbal Paz, Ruth E. Ley
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Bidirectional linkage of DNA barcodes for the multiplexed mapping of higher-order protein interactions in cells Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-06-19 Yu Liu, Noah R. Sundah, Nicholas R. Y. Ho, Wan Xiang Shen, Yun Xu, Auginia Natalia, Zhonglang Yu, Ju Ee Seet, Ching Wan Chan, Tze Ping Loh, Brian Y. Lim, Huilin Shao
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A pathologist–AI collaboration framework for enhancing diagnostic accuracies and efficiencies Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-06-19 Zhi Huang, Eric Yang, Jeanne Shen, Dita Gratzinger, Frederick Eyerer, Brooke Liang, Jeffrey Nirschl, David Bingham, Alex M. Dussaq, Christian Kunder, Rebecca Rojansky, Aubre Gilbert, Alexandra L. Chang-Graham, Brooke E. Howitt, Ying Liu, Emily E. Ryan, Troy B. Tenney, Xiaoming Zhang, Ann Folkins, Edward J. Fox, Kathleen S. Montine, Thomas J. Montine, James Zou
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Development and applications of lipid hydrophilic headgroups for nucleic acid therapy Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-19 Wanting Ma, Xingxing Fu, Tianyi Zhao, Yanfei Qi, Shubiao Zhang, Yinan Zhao
Nucleic acid therapy is currently the most promising method for treating tumors and genetic diseases and for preventing infectious diseases. However, the biggest obstacle to this therapy is delivery of the nucleic acids to the target site, which requires overcoming problems such as capture by the immune system, the need to penetrate biofilms, and degradation of nucleic acid performance. Designing suitable
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Cordyceps militaris: A novel mushroom platform for metabolic engineering Biotechnol. Adv. (IF 12.1) Pub Date : 2024-06-19 Jiapeng Zeng, Yue Zhou, Mengdi Lyu, Xinchang Huang, Muyun Xie, Mingtao Huang, Bai-Xiong Chen, Tao Wei
, widely recognized as a medicinal and edible mushroom in East Asia, contains a variety of bioactive compounds, including cordycepin (COR), pentostatin (PTN) and other high-value compounds. This review explores the potential of developing as a cell factory for the production of high-value chemicals and nutrients. This review comprehensively summarizes the fermentation advantages, metabolic networks
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Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum Metab. Eng. (IF 6.8) Pub Date : 2024-06-18 Kai Li, Cheng Li, Chen-Guang Liu, Xin-Qing Zhao, Ruiwen Ou, Charles A. Swofford, Feng-Wu Bai, Gregory Stephanopoulos, Anthony J. Sinskey
Effective utilization of glucose, xylose, and acetate, common carbon sources in lignocellulose hydrolysate, can boost biomanufacturing economics. However, carbon leaks into biomass biosynthesis pathways instead of the intended target product remain to be optimized. This study aimed to enhance α-carotene production by optimizing glucose, xylose, and acetate utilization in a high-efficiency cell factory
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A long-term growth stable Halomonas sp. deleted with multiple transposases guided by its metabolic network model Halo-ecGEM Metab. Eng. (IF 6.8) Pub Date : 2024-06-18 Lizhan Zhang, Jian-Wen Ye, Gang Li, Helen Park, Hao Luo, Yina Lin, Shaowei Li, Weinan Yang, Yuying Guan, Fuqing Wu, Wuzhe Huang, Qiong Wu, Nigel S. Scrutton, Jens Nielsen, Guo-Qiang Chen
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Engineered yeast produces the plant-based vaccine adjuvant QS-21 Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17 Iris Marchal
Extracting pharmacological compounds from plants can be ecologically damaging and often results in low yields, which limits their broad use. For instance, the supply of the potent vaccine adjuvant QS-21 is dependent on laborious extraction from the bark of the Chilean soapbark tree. Writing in Nature, Liu et al. describe an alternative method to produce QS-21 and its derivatives at scale using engineered
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OpenFold provides insights into AlphaFold2’s learning behavior Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17 Iris Marchal
Despite the immense utility of AlphaFold2 in predicting protein structure, the official implementation excludes code for its training procedure and the associated required data. This makes it difficult to study the model’s learning behavior and to create variants that can perform new tasks. Writing in Nature Methods, AlQuraishi and colleagues now report OpenFold, a trainable and open-source implementation
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Honey without bees Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
Honey has been a prized food since ancient times. A complex blend of sugars, mostly fructose and glucose, and small amounts of other sugars, proteins, amino acids, vitamins and minerals, honey is a $8.94 billion market, and its popularity continues to grow. But over the last decade, honeybees (Apis mellifera) have been hindered from making this natural product by disappearing grassland, herbicides
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A vaccine strategy for inducing broadly neutralizing antibodies against HIV Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17 Iris Marchal
An effective HIV vaccine must induce broadly neutralizing antibodies (bnAbs) that target the most conserved sites of the HIV envelope, a feat that has not yet been achieved in humans. Germline-targeting vaccine design holds promise by using immunogens to prime naive B cells for bnAb precursor activation and then boosting their maturation. A major challenge for this approach is that antibody–antigen
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First-in-class T cell engager approved for lung cancer Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
Amgen’s T cell engager Imdelltra (tarlatamab) has gained FDA accelerated approval for an aggressive type of cell lung cancer that is notoriously difficult to treat. The bispecific antibody is designed to bind both delta-like ligand 3 (DLL3) on small cell lung cancer (SCLC) cells and CD3 on T cells. Pharma companies have long considered DDL3 a promising target in oncology because healthy cells express
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Forum: Anikeeva, Courtine and Bloch Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
On this episode, Chief Editor Barbara Cheifet speaks with Polina Anikeeva from MIT, Grégoire Courtine from EPFL, and Jocelyn Bloch, a neurosurgeon at Lausanne University Hospital. These three leaders in the field of neurotechnologies discuss new devices that help us learn how our brain works, implantable brain–computer interfaces that are helping patients with neurological disorders walk again, and
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Biotech news from around the world Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
The kingdom pledges $500 million to the Bill & Melinda Gates Foundation to help fight polio. The funding, which helps close the funding gap in the $4.8 billion budget for the Global Polio Eradication Initiative, will be spread out over five years and makes Saudi Arabia one of the biggest national donors, along with the United States. A pilot project by the government of New South Wales has produced
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Gene therapy restores hearing Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
Regeneron Pharmaceuticals has released preliminary data from the first two patients treated with the investigational gene therapy DB-OTO. At the American Society for Gene and Cell Therapy (ASGCT) annual meeting in May, the company disclosed that, during the CHORD clinical trial, designed to evaluate safety and tolerability, the gene therapy improved hearing in two children with profound genetic deafness
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Cancer diagnostics Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
Recent patents relating to systems and methods for cancer detection and diagnosis.
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Bird flu in wastewater Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
The Centers for Disease Control and Prevention (CDC) is testing wastewater across the United States to track the spread of influenza virus, including the avian influenza A virus H5N1 outbreak. The CDC and its partners are sampling wastewater from 689 sites to detect influenza A virus by quantitative PCR. The surveillance and reporting system also includes monkeypox and SARS-CoV-2. Data from May 5–18
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B cells as drug factories Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
Companies are engineering B cells to produce gene-encoded medicines, but clinical proof of concept is needed to convince the doubters that this platform can deliver.
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People Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17
Recent moves of note in and around the biotech and pharma industries.
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Barriers and enablers to the effective implementation of omics research in low- and middle-income countries Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17 Jacus S. Nacis, Patrick Kamande, Alemayehu Teklu Toni, Evans Mudibo, Robert Musyimi, Siam Popluechai, Trenton Dailey-Chwalibóg, Wieger Voskuijl, Genevieve Dable-Tupas, Abu Sadat Mohammad Sayeem Bin Shahid, Neil Andrew Bascos, Farzana Afroze, Mohammod Jobayer Chisti, Benson Singa, Moses Ngari, Caroline Tigoi, Gomezgani Mhango, Harry Freitag, Isabel Potani, John Mukisa, Amir Kirolos, Kuda Mutasa, Lionel
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The sufficiency of disclosure of medical artificial intelligence patents Nat. Biotechnol. (IF 33.1) Pub Date : 2024-06-17 Mateo Aboy, W. Nicholson Price, Seth Raker, Kathleen Liddell
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Enhancement of erythropoietic output by Cas9-mediated insertion of a natural variant in haematopoietic stem and progenitor cells Nat. Biomed. Eng. (IF 26.8) Pub Date : 2024-06-17 Sofia E. Luna, Joab Camarena, Jessica P. Hampton, Kiran R. Majeti, Carsten T. Charlesworth, Eric Soupene, Sridhar Selvaraj, Kun Jia, Vivien A. Sheehan, M. Kyle Cromer, Matthew H. Porteus
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Metabolic engineering of Pichia pastoris for overproduction of cis-trans nepetalactol Metab. Eng. (IF 6.8) Pub Date : 2024-06-17 Cuifang Ye, Mengxin Li, Jucan Gao, Yimeng Zuo, Feng Xiao, Xiaojing Jiang, Jintao Cheng, Lei Huang, Zhinan Xu, Jiazhang Lian
Monoterpene indole alkaloids (MIAs) are a group of plant-derived natural products with high-value medicinal properties. However, their availability for clinical application is limited due to challenges in plant extraction. Microbial production has emerged as a promising strategy to meet the clinical demands for MIAs. The biosynthetic pathway of nepetalactol, which serves as the universal iridoid scaffold
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Improving the genetic stability of bacterial growth control for long-term bioproduction Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-15 Thibault Clavier, Corinne Pinel, Hidde de Jong, Johannes Geiselmann
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A multicrosslinked network composite hydrogel scaffold based on DLP photocuring printing for nasal cartilage repair Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-14 Wendan Jia, Zixian Liu, Lei Sun, Yanyan Cao, Zhizhong Shen, Meng Li, Yang An, Hulin Zhang, Shengbo Sang
Natural hydrogels are widely employed in tissue engineering and have excellent biodegradability and biocompatibility. Unfortunately, the utilization of such hydrogels in the field of three-dimensional (3D) printing nasal cartilage is constrained by their subpar mechanical characteristics. In this study, we provide a multicrosslinked network hybrid ink made of photocurable gelatin, hyaluronic acid,
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Commitment of human mesenchymal stromal cells towards ACL fibroblast differentiation upon rAAV-mediated FGF-2 and TGF-β overexpression using pNaSS-grafted PCL films Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-14 Meret Stehle, Mahnaz Amini, Jagadeesh K. Venkatesan, Wei Liu, Dan Wang, Tuan N. Nguyen, Amélie Leroux, Henning Madry, Véronique Migonney, Magali Cucchiarini
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Genome-scale models of metabolism and expression predict the metabolic burden of recombinant protein expression Metab. Eng. (IF 6.8) Pub Date : 2024-06-15 Omid Oftadeh, Vassily Hatzimanikatis
The production of recombinant proteins in a host using synthetic constructs such as plasmids comes at the cost of detrimental effects such as reduced growth, energetic inefficiencies, and other stress responses, collectively known as metabolic burden. Increasing the number of copies of the foreign gene increases the metabolic load but increases the expression of the foreign protein. Thus, there is
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Lentiviral vector determinants of anion-exchange chromatography elution heterogeneity Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-14 George Pamenter, Lee Davies, Ciaran Lamont, Danyal Rahim, Carol Knevelman, James Miskin, Kyriacos Mitrophanous, Duygu Dikicioglu, Daniel G. Bracewell
The demand for Lentiviral Vector (LV) drug substance is increasing. However, primary capture using convective anion-exchange chromatography remains a significant manufacturing challenge. This stems from a poor understanding of the complex adsorption behaviors linked to LVs intricate and variable structure, such as high binding heterogeneity which is typically characterized by a gradient elution profile
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Enhancing cell-based therapies with synthetic gene circuits responsive to molecular stimuli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-12 Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
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