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GA dynamics governing nodulation revealed using GIBBERELLIN PERCEPTION SENSOR 2 in Medicago truncatula lateral organs Plant Cell (IF 10.0) Pub Date : 2024-07-16 Colleen Drapek, Annalisa Rizza, Nadiatul A Mohd-Radzman, Katharina Schiessl, Fabio Dos Santos Barbosa, Jiangqi Wen, Giles E D Oldroyd, Alexander M Jones
During nutrient scarcity, plants can adapt their developmental strategy to maximize their chance of survival. Such plasticity in development is underpinned by hormonal regulation, which mediates the relationship between environmental cues and developmental outputs. In legumes, endosymbiosis with nitrogen fixing bacteria (rhizobia) is a key adaptation for supplying the plant with nitrogen in the form
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PHOSPHATASE 2A dephosphorylates PHYTOCHROME INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-07-12 Xingbo Cai, Sanghwa Lee, Andrea Paola Gomej Jaime, Wenqiang Tang, Yu Sun, Enamul Huq
The phytochrome (phy) family of sensory photoreceptors modulates developmental programs in response to ambient light. Phys also control gene expression in part by directly interacting with the bHLH class of transcription factors, PHYTOCHROME-INTERACTING FACTORS (PIFs), and inducing their rapid phosphorylation and degradation. Several kinases have been shown to phosphorylate PIFs and promote their degradation
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The lowdown on breakdown: Open questions in plant proteolysis Plant Cell (IF 10.0) Pub Date : 2024-07-09 Nancy A Eckardt, Tamar Avin-Wittenberg, Diane C Bassham, Poyu Chen, Qian Chen, Jun Fang, Pascal Genschik, Abi S Ghifari, Angelica M Guercio, Daniel J Gibbs, Maren Heese, R Paul Jarvis, Simon Michaeli, Monika W Murcha, Sergey Mursalimov, Sandra Noir, Malathy Palayam, Bruno Peixoto, Pedro L Rodriguez, Andreas Schaller, Arp Schnittger, Giovanna Serino, Nitzan Shabek, Annick Stintzi, Frederica L Theodoulou
Proteolysis, including post-translational proteolytic processing as well as protein degradation and amino acid recycling, is an essential component of the growth and development of living organisms. In this article, experts in plant proteolysis pose and discuss compelling open questions in their areas of research. Topics covered include the role of proteolysis in the cell cycle, DNA damage response
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The H1/H5 domain contributes to OsTRBF2 phase separation and gene repression during rice development Plant Cell (IF 10.0) Pub Date : 2024-07-08 Hua Xuan, Yanzhuo Li, Yue Liu, Jingze Zhao, Jianhao Chen, Nan Shi, Yulu Zhou, Limin Pi, Shaoqing Li, Guoyong Xu, Hongchun Yang
Transcription factors (TFs) tightly control plant development by regulating gene expression. The phase separation of TFs plays a vital role in gene regulation. Many plant TFs have the potential to form phase-separated protein condensates; however, little is known about which TFs are regulated by phase separation and how it affects their roles in plant development. Here, we report that the rice (Oryza
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The stromal side of the cytochrome b6f complex regulates state transitions Plant Cell (IF 10.0) Pub Date : 2024-07-04 Alexis Riché, Louis Dumas, Soazig Malesinski, Guillaume Bossan, Céline Madigou, Francesca Zito, Jean Alric
In oxygenic photosynthesis, state transitions distribute light energy between Photosystem I and Photosystem II. This regulation involves reduction of the plastoquinone pool, activation of the State Transitions 7 (STT7) protein kinase by the cytochrome b6f complex, and phosphorylation and migration of Light Harvesting Complex II (LHCII). Here, we show that in Chlamydomonas reinhardtii, the C-terminus
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RESISTANCE TO PHYTOPHTHORA1 promotes cytochrome b559 formation during early photosystem II biogenesis in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-07-04 Li-Ping Che, Junxiang Ruan, Qiang Xin, Lin Zhang, Fudan Gao, Lujuan Cai, Jianing Zhang, Shiwei Chen, Hui Zhang, Jean-David Rochaix, Lianwei Peng
As an essential intrinsic component of photosystem II (PSII) in all oxygenic photosynthetic organisms, heme-bridged heterodimer cytochrome b559 (Cyt b559) plays critical roles in protection and assembly of PSII. However, the underlying mechanisms of Cyt b559 assembly are largely unclear. Here, we characterized the Arabidopsis (Arabidopsis thaliana) rph1 (resistance to Phytophthora1) mutant, which was
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A cargo sorting receptor mediates chloroplast protein trafficking through the secretory pathway Plant Cell (IF 10.0) Pub Date : 2024-07-04 Jinling Liu, Hong Chen, Li Liu, Xiangzhao Meng, Qianwen Liu, Qinyi Ye, Jiangqi Wen, Tao Wang, Jiangli Dong
Nucleus-encoded chloroplast proteins can be transported via the secretory pathway. The molecular mechanisms underlying the trafficking of chloroplast proteins between the intracellular compartments are largely unclear, and a cargo sorting receptor has not previously been identified in the secretory pathway. Here we report a cargo sorting receptor that is specifically present in Viridiplantae and mediates
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Two PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEINs are essential for management of the coenzyme pyridoxal 5'-phosphate in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-07-02 Peter Farkas, Teresa B Fitzpatrick
Coenzyme management is important for homeostasis of the pool of active metabolic enzymes. The coenzyme pyridoxal 5'-phosphate (PLP) is involved in diverse enzyme reactions including amino acid and hormone metabolism. Regulatory proteins that contribute to PLP homeostasis remain to be explored in plants. Here we demonstrate the importance of proteins annotated as PLP HOMEOSTASIS PROTEINs (PLPHPs) for
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CELLULOSE SYNTHASE-LIKE C proteins modulate cell wall establishment during ethylene-mediated root growth inhibition in rice Plant Cell (IF 10.0) Pub Date : 2024-06-29 Yang Zhou, Yi-Hong Gao, Bao-Cai Zhang, Han-Lei Yang, Yan-Bao Tian, Yi-Hua Huang, Cui-Cui Yin, Jian-Jun Tao, Wei Wei, Wan-Ke Zhang, Shou-Yi Chen, Yi-Hua Zhou, Jin-Song Zhang
The cell wall shapes plant cell morphogenesis and affects the plasticity of organ growth. However, the way in which cell wall establishment is regulated by ethylene remains largely elusive. Here, by analyzing cell wall patterns, cell wall composition and gene expression in rice (Oryza sativa, L.) roots, we found that ethylene induces cell wall thickening and the expression of cell wall synthesis-related
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Alternative splicing of a potato disease resistance gene maintains homeostasis between growth and immunity Plant Cell (IF 10.0) Pub Date : 2024-06-28 Biying Sun, Jie Huang, Liang Kong, Chuyun Gao, Fei Zhao, Jiayong Shen, Tian Wang, Kangping Li, Luyao Wang, Yuanchao Wang, Dennis A Halterman, Suomeng Dong
Plants possess a robust and sophisticated innate immune system against pathogens and must balance growth with rapid pathogen detection and defense. The intracellular receptors with nucleotide-binding leucine-rich repeat (NLR) motifs recognize pathogen-derived effector proteins and thereby trigger the immune response. The expression of genes encoding NLR receptors is precisely controlled in multifaceted
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Molecular mechanisms underlying gene regulatory variation of maize metabolic traits Plant Cell (IF 10.0) Pub Date : 2024-06-26 Yi-Hsuan Chu, Yun Sun Lee, Fabio Gomez-Cano, Lina Gomez-Cano, Peng Zhou, Andrea I Doseff, Nathan Springer, Erich Grotewold
Variation in gene expression levels is pervasive among individuals and races or varieties, and has substantial agronomic consequences, for example, by contributing to hybrid vigor. Gene expression level variation results from mutations in regulatory sequences (cis) and/or transcription factor (TF) activity (trans), but the mechanisms underlying cis and/or trans-regulatory variation of complex phenotypes
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The U-Box E3 Ubiquitin Ligase PUB35 Negatively Regulates ABA Signaling through AFP1-mediated Degradation of ABI5 Plant Cell (IF 10.0) Pub Date : 2024-06-26 Chang Du, Meng Liu, Yujie Yan, Xiaoyu Guo, Xiuping Cao, Yuzhe Jiao, Jiexuan Zheng, Yanchun Ma, Yuting Xie, Hongbo Li, Chengwei Yang, Caiji Gao, Qingzhen Zhao, Zhonghui Zhang
Abscisic acid (ABA) signaling is crucial for plant responses to various abiotic stresses. The Arabidopsis (Arabidopsis thaliana) transcription factor ABA INSENSITIVE 5 (ABI5) is a central regulator of ABA signaling. ABI5 BINDING PROTEIN 1 (AFP1) interacts with ABI5 and facilitates its 26S-proteasome-mediated degradation, although the detailed mechanism has remained unclear. Here, we report that an
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Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation Plant Cell (IF 10.0) Pub Date : 2024-06-26 Srayan Ghosh, Macarena Mellado Sanchez, Kawinnat Sue-Ob, Dipan Roy, Andrew Jones, Miguel A Blazquez, Ari Sadanandom
SUMO modification is part of the spectrum of Ubiquitin-like (UBL) systems that give rise to proteoform complexity through post-translational modifications (PTMs). Proteoforms are essential modifiers of cell signaling for plant adaptation to changing environments. Exploration of the evolutionary emergence of Ubiquitin-like (UBL) systems unveils their origin from prokaryotes where it is linked to the
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Bioengineering secreted proteases convert divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors Plant Cell (IF 10.0) Pub Date : 2024-06-26 Jiorgos Kourelis, Mariana Schuster, Fatih Demir, Oliver Mattinson, Sonja Krauter, Parvinderdeep S Kahlon, Ruby O’Grady, Samantha Royston, Ana Lucía Bravo-Cazar, Brian C Mooney, Pitter F Huesgen, Sophien Kamoun, Renier A L van der Hoorn
Secreted immune proteases Rcr3 (Required for Cladosporium resistance-3) and Pip1 (Phytophthora- inhibited protease-1) of tomato (Solanum lycopersicum) are both inhibited by Avr2 from the fungal plant pathogen Cladosporium fulvum. However, only Rcr3 acts as a decoy co-receptor that detects Avr2 in the presence of the Cf-2 immune receptor. Here, we identified crucial residues in tomato Rcr3 that are
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NRC immune receptor networks show diversified hierarchical genetic architecture across plant lineages Plant Cell (IF 10.0) Pub Date : 2024-06-26 Foong-Jing Goh, Ching-Yi Huang, Lida Derevnina, Chih-Hang Wu
Plants’ complex immune systems include nucleotide-binding domain and leucine-rich repeat-containing (NLR) proteins, which help recognize invading pathogens. In solanaceous plants, the NRC (NLR required for cell death) family includes helper NLRs that form a complex genetic network with multiple sensor NLRs to provide resistance against pathogens. However, the evolution and function of NRC networks
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Population-level exploration of alternative splicing and its unique role in controlling agronomic traits of rice Plant Cell (IF 10.0) Pub Date : 2024-06-25 Hong Zhang, Wu Chen, De Zhu, Bintao Zhang, Qiang Xu, Chuanlin Shi, Huiying He, Xiaofan Dai, Yilin Li, Wenchuang He, Yang Lv, Longbo Yang, Xinglan Cao, Yan Cui, Yue Leng, Hua Wei, Xiangpei Liu, Bin Zhang, Xianmeng Wang, Mingliang Guo, Zhipeng Zhang, Xiaoxia Li, Congcong Liu, Qiaoling Yuan, Tianyi Wang, Xiaoman Yu, Hongge Qian, Qianqian Zhang, Dandan Chen, Guanjing Hu, Qian Qian, Lianguang Shang
Alternative splicing (AS) plays crucial roles in regulating various biological processes in plants. However, the genetic mechanisms underlying AS and its role in controlling important agronomic traits in rice (Oryza sativa) remain poorly understood. In this study, we explored AS in rice leaves and panicles using the rice minicore collection. Our analysis revealed a high level of transcript isoform
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NIN-LIKE PROTEIN3.2 inhibits repressor Aux/IAA14 expression and enhances root biomass in maize seedlings under low nitrogen Plant Cell (IF 10.0) Pub Date : 2024-06-25 Ruifeng Wang, Yanting Zhong, Jienan Han, Liangliang Huang, Yongqi Wang, Xionggao Shi, Mengfei Li, Yao Zhuang, Wei Ren, Xiaoting Liu, Huairong Cao, Beibei Xin, Jinsheng Lai, Limei Chen, Fanjun Chen, Lixing Yuan, Yi Wang, Xuexian Li
Plants generally enhance their root growth in the form of greater biomass and/or root length to boost nutrient uptake in response to short-term low nitrogen (LN). However, the underlying mechanisms of short-term LN-mediated root growth remain largely elusive. Our genome-wide association study, haplotype analysis, and phenotyping of transgenic plants showed that the crucial nitrate signaling component
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The SMXL8-AGL9 module mediates crosstalk between strigolactone and gibberellin to regulate strigolactone-induced anthocyanin biosynthesis in apple Plant Cell (IF 10.0) Pub Date : 2024-06-25 Jian-Ping An, Lei Zhao, Yun-Peng Cao, Di Ai, Miao-Yi Li, Chun-Xiang You, Yuepeng Han
Although the strigolactone (SL) signaling pathway and SL-mediated anthocyanin biosynthesis have been reported, the molecular association between SL signaling and anthocyanin biosynthesis remains unclear. In this study, we identified the SL signal transduction pathway associated with anthocyanin biosynthesis and the crosstalk between gibberellin (GA) and SL signaling in apple (Malus × domestica). ELONGATED
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Natural variation in response to combined water and nitrogen deficiencies in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-06-25 Zeyun Xue, Marina Ferrand, Elodie Gilbault, Olivier Zurfluh, Gilles Clément, Anne Marmagne, Stéphanie Huguet, José M Jiménez-Gómez, Anne Krapp, Christian Meyer, Olivier Loudet
Understanding plant responses to individual stresses does not mean that we understand real world situations, where stresses usually combine and interact. These interactions arise at different levels, from stress exposure to the molecular networks of the stress response. Here, we built an in-depth multi-omics description of plant responses to mild water (W) and nitrogen (N) limitations, either individually
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Differential phosphorylation of Ca2+-permeable channel CNGC20 modulates calcium-mediated freezing tolerance in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-06-14 Yue Peng, Yuhang Ming, Bochen Jiang, Xiuyue Zhang, Diyi Fu, Qihong Lin, Xiaoyan Zhang, Yi Wang, Yiting Shi, Zhizhong Gong, Yanglin Ding, Shuhua Yang
Plants respond to cold stress at multiple levels, including increasing cytosolic calcium (Ca2+) influx and triggering the expression of cold-responsive genes. Here we show that the Ca2+-permeable channel CYCLIC NUCLEOTIDE GATED CHANNEL20 (CNGC20) positively regulates freezing tolerance in Arabidopsis (Arabidopsis thaliana) by mediating cold-induced Ca2+ influx. Moreover, we demonstrate that the leucine-rich
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Multi-transcriptomics identifies targets of the endoribonuclease DNE1 and highlights its coordination with decapping Plant Cell (IF 10.0) Pub Date : 2024-06-13 Aude Pouclet, David Pflieger, Rémy Merret, Marie-Christine Carpentier, Marlene Schiaffini, Hélène Zuber, Dominique Gagliardi, Damien Garcia
Decapping is a crucial step in mRNA degradation in eucaryotes and requires the formation of a holoenzyme complex between the decapping enzyme DECAPPING 2 (DCP2) and the decapping enhancer DCP1. In Arabidopsis (Arabidopsis thaliana), DCP1-ASSOCIATED NYN ENDORIBONUCLEASE 1 (DNE1) is a direct protein partner of DCP1. The function of both DNE1 and decapping are necessary to maintain phyllotaxis, the regularity
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Transcription factors BZR1 and PAP1 cooperate to promote anthocyanin biosynthesis in Arabidopsis shoots Plant Cell (IF 10.0) Pub Date : 2024-06-13 Se-Hwa Lee, So-Hee Kim, Tae-Ki Park, Young-Pil Kim, Jin-Won Lee, Tae-Wuk Kim
Anthocyanins play critical roles in protecting plant tissues against diverse stresses. The complicated regulatory networks induced by various environmental factors modulate the homeostatic level of anthocyanins. Here, we show that anthocyanin accumulation is induced by brassinosteroids (BRs) in Arabidopsis (Arabidopsis thaliana) shoots and shed light on the underlying regulatory mechanism. We observed
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The MdHSC70-MdWRKY75 module mediates basal apple thermotolerance by regulating the expression of heat shock factor genes Plant Cell (IF 10.0) Pub Date : 2024-06-12 Zhijun Zhang, Chao Yang, Jing Xi, Yuting Wang, Jing Guo, Qianwei Liu, Yusong Liu, Yang Ma, Jing Zhang, Fengwang Ma, Chao Li
Heat stress severely restricts the growth and fruit development of apple (Malus domestica). Little is known about the involvement of WRKY proteins in the heat tolerance mechanism in apple. In this study, we found that the apple transcription factor MdWRKY75 responds to heat and positively regulates basal thermotolerance. Apple plants that overexpressed MdWRKY75 were more tolerant to heat stress, while
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Three Arabidopsis UMP kinases have different roles in pyrimidine nucleotide biosynthesis and (deoxy)CMP salvage Plant Cell (IF 10.0) Pub Date : 2024-06-12 Jannis Rinne, Markus Niehaus, Nieves Medina-Escobar, Henryk Straube, Frank Schaarschmidt, Nils Rugen, Hans-Peter Braun, Marco Herde, Claus-Peter Witte
Pyrimidine nucleotide monophosphate biosynthesis ends in the cytosol with uridine monophosphate (UMP). UMP phosphorylation to uridine diphosphate (UDP) by UMP KINASEs (UMKs) is required for the generation of all pyrimidine (deoxy)nucleoside triphosphates as building blocks for nucleic acids and central metabolites like UDP-glucose. The Arabidopsis (Arabidopsis thaliana) genome encodes five UMKs and
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HIGH PLOIDY2-mediated SUMOylation of transcription factor ARR1 controls two-component signaling in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-05-31 Na Young Kang, Min-Jung Kim, Seon Jeong, Sun Young Moon, Jin Sun Kim, Jin Jeon, Boyoung Lee, Mi Rha Lee, Jungmook Kim
Cytokinins regulate plant growth, development, and responses to environmental stresses such as cold via phosphorelay from cytokinin receptors to the ARABIDOPSIS RESPONSE REGULATORs (ARRs). However, the molecular mechanisms underlying the activation of type-B ARR transcriptional activity in Arabidopsis (Arabidopsis thaliana) remain unclear. Here, we show that the E3 SUMO ligase HIGH PLOIDY2 SUMOylates
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The plant rhabdovirus viroporin P9 facilitates insect-mediated virus transmission in barley Plant Cell (IF 10.0) Pub Date : 2024-05-31 Qiang Gao, Ying Zang, Ji-Hui Qiao, Zong-Ying Zhang, Ying Wang, Cheng-Gui Han, Xian-Bing Wang
Potassium (K+) plays crucial roles in both plant development and immunity. However, the function of K+ in plant–virus interactions remains largely unknown. Here, we utilized Barley yellow striate mosaic virus (BYSMV), an insect-transmitted plant cytorhabdovirus, to investigate the interplay between viral infection and plant K+ homeostasis. The BYSMV accessory P9 protein exhibits viroporin activity
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The Brassinosteroid Receptor StBRI1 Promotes Tuber Development by Enhancing Plasma Membrane H+-ATPase Activity in Potato Plant Cell (IF 10.0) Pub Date : 2024-05-31 Rui Deng, Shuhua Huang, Jia Du, Dan Luo, Jianwei Liu, Yan Zhao, Chongyang Zheng, Tiantian Lei, Qi Li, Siwei Zhang, Meng Jiang, Tong Jin, Dehai Liu, Shufen Wang, Yanfeng Zhang, Xiaofeng Wang
The brassinosteroid (BR) receptor BRASSINOSTEROID-INSENSITIVE 1 (BRI1) plays a critical role in plant growth and development. Although much is known about how BR signaling regulates growth and development in many crop species, the role of StBRI1 in regulating potato (Solanum tuberosum) tuber development is not well understood. To address this question, a series of comprehensive genetic and biochemical
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Computational Photosynthesis (ComPhot): Simulation-Based Learning Platform to Study Photosynthesis Plant Cell (IF 10.0) Pub Date : 2024-05-31 Sarah Philipps, Tobias Pfennig, Elouën Corvest, Marvin van Aalst, Lisa Fürtauer, Anna Matuszyńska
Studies show the advantage of active versus passive learning formats in delivering complicated concepts (Minocha and Clarke, 2009; Pluta et al., 2013). Hence, interactive teaching tools are not only more often positively evaluated by students but also contribute to better life-long teaching outcomes (Ang et al., 2021). Following this evidence, we created ComPhot, a stand-alone learning platform for
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Multi-omic analyses reveal key sectors of jasmonate-mediated defense responses in rice Plant Cell (IF 10.0) Pub Date : 2024-05-27 Yumeng Chen, Gaochen Jin, Mengyu Liu, Lanlan Wang, Yonggen Lou, Ian Baldwin, Ran Li
The phytohormone jasmonate (JA) plays a central role in plant defenses against biotic stressors. However, our knowledge of the JA signaling pathway in rice (Oryza sativa) remains incomplete. Here, we integrated multi-omic data from three tissues to characterize the functional modules involved in organizing JA-responsive genes. In the core regulatory sector, MYC2 transcription factor transcriptional
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Antisense transcription from stress-responsive transcription factors fine-tunes the cold response in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-05-27 Shiv Kumar Meena, Marti Quevedo, Sarah Muniz Nardeli, Clément Verez, Susheel Sagar Bhat, Vasiliki Zacharaki, Peter Kindgren
Transcription of antisense long noncoding RNAs (lncRNAs) occurs pervasively across eukaryotic genomes. Only a few antisense lncRNAs have been characterized and shown to control biological processes, albeit with idiosyncratic regulatory mechanisms. Thus, we largely lack knowledge about the general role of antisense transcription in eukaryotic organisms. Here, we characterized genes with antisense transcription
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COI1 F-box proteins regulate DELLA protein levels, growth, and photosynthetic efficiency in maize Plant Cell (IF 10.0) Pub Date : 2024-05-27 Leila Feiz, Christine Shyu, Shan Wu, Kevin R Ahern, Iram Gull, Ying Rong, Caroline J Artymowicz, Miguel A Piñeros, Zhangjun Fei, Thomas P Brutnell, Georg Jander
The F-box protein Coronatine Insensitive (COI) is a receptor for the jasmonic acid signaling pathway in plants. To investigate the functions of the six maize (Zea mays) COI proteins (COI1a, COI1b, COI1c, COI1d, COI2a, and COI2b), we generated single, double, and quadruple loss-of-function mutants. The pollen of the coi2a coi2b double mutant was inviable. The coi1 quadruple mutant (coi1-4x) exhibited
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A viral small interfering RNA-host plant mRNA pathway modulates virus-induced drought tolerance by enhancing autophagy Plant Cell (IF 10.0) Pub Date : 2024-05-27 Xinyang Wu, Shuting Chen, Zixin Zhang, Weixin Zhou, Ting Sun, Kang Ning, Min Xu, Xubo Ke, Pei Xu
Virus-induced drought tolerance presents a fascinating facet of biotic-abiotic interaction in plants, yet its molecular intricacies remain unclear. Our study shows that cowpea mild mottle virus (CPMMV) infection enhances drought tolerance in common bean (Phaseolus vulgaris) plants through a virus-derived small interfering RNA (vsiRNA)-activated autophagy pathway. Specifically, a 21-bp vsiRNA originating
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A pgr5 suppressor screen uncovers two distinct suppression mechanisms and links cytochrome b6f complex stability to PGR5 Plant Cell (IF 10.0) Pub Date : 2024-05-23 Jan-Ferdinand Penzler, Belén Naranjo, Sabrina Walz, Giada Marino, Tatjana Kleine, Dario Leister
PROTON GRADIENT REGULATION5 (PGR5) is thought to promote cyclic electron flow, and its deficiency impairs photosynthetic control and increases photosensitivity of photosystem (PS) I, leading to seedling lethality under fluctuating light (FL). By screening for Arabidopsis (Arabidopsis thaliana) suppressor mutations that rescue the seedling lethality of pgr5 plants under FL, we identified a portfolio
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SEPALLATA-driven MADS transcription factor tetramerization is required for inner whorl floral organ development Plant Cell (IF 10.0) Pub Date : 2024-05-21 Veronique Hugouvieux, Romain Blanc-Mathieu, Aline Janeau, Michel Paul, Jeremy Lucas, Xiaocai Xu, Hailong Ye, Xuelei Lai, Sarah LeHir, Audrey Guillotin, Antonin Galien, Wenhao Yan, Max Nanao, Kerstin Kaufmann, François Parcy, Chloe Zubieta
MADS transcription factors are master regulators of plant reproduction and flower development. The SEPALLATA (SEP) subfamily of MADS transcription factors is required for the development of floral organs and plays roles in inflorescence architecture and development of the floral meristem. SEPALLATAs act as organizers of MADS complexes, forming both heterodimers and heterotetramers in vitro. To date
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Alternative electron pathways of photosynthesis power green algal CO2 capture Plant Cell (IF 10.0) Pub Date : 2024-05-13 Gilles Peltier, Carolyne Stoffel, Justin Findinier, Sai Kiran Madireddi, Ousmane Dao, Virginie Epting, Amélie Morin, Arthur Grossman, Yonghua Li-Beisson, Adrien Burlacot
Microalgae contribute to about half of global net photosynthesis, which converts sunlight into the chemical energy (ATP and NADPH) used to transform CO2 into biomass. Alternative electron pathways of photosynthesis have been proposed to generate additional ATP that is required to sustain CO2 fixation. However, the relative importance of each alternative pathway remains elusive. Here, we dissect and
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A transthyretin-like protein acts downstream of miR397 and LACCASE to regulate grain yield in rice Plant Cell (IF 10.0) Pub Date : 2024-05-13 Yang Yu, Rui-Rui He, Lu Yang, Yan-Zhao Feng, Jiao Xue, Qing Liu, Yan-Fei Zhou, Meng-Qi Lei, Yu-Chan Zhang, Jian-Ping Lian, Yue-Qin Chen
Increasing grain yield is a major goal of breeders due to the rising global demand for food. We previously reported that the miR397–LACCASE (OsLAC) module regulates brassinosteroid (BR) signaling and grain yield in rice (Oryza sativa). However, the precise roles of laccase enzymes in the BR pathway remain unclear. Here, we report that OsLAC controls grain yield by preventing the turnover of TRANSTHYRETIN-LIKE
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The OsMOB1A–OsSTK38 kinase complex phosphorylates CYCLIN C, controlling grain size and weight in rice Plant Cell (IF 10.0) Pub Date : 2024-05-09 Guoxin Chen, Jiabei Gao, Suting Wu, Yuan Chang, Zhenhua Chen, Jing Sun, Liying Zhang, Jinxia Wu, Xuehui Sun, William Paul Quick, Xuean Cui, Zhiguo Zhang, Tiegang Lu
Grain size and weight are crucial yield-related traits in rice (Oryza sativa). Although certain key genes associated with rice grain size and weight have been successfully cloned, the molecular mechanisms underlying grain size and weight regulation remain elusive. Here, we identified a molecular pathway regulating grain size and weight in rice involving the MPS ONE BINDER KINASE ACTIVATOR-LIKE 1A–
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The microRNA408–plantacyanin module balances plant growth and drought resistance by regulating reactive oxygen species homeostasis in guard cells Plant Cell (IF 10.0) Pub Date : 2024-05-09 Yanzhi Yang, Lei Xu, Chen Hao, Miaomiao Wan, Yihan Tao, Yan Zhuang, Yanning Su, Lei Li
The conserved microRNA (miRNA) miR408 enhances photosynthesis and compromises stress tolerance in multiple plants, but the cellular mechanism underlying its function remains largely unclear. Here, we show that in Arabidopsis (Arabidopsis thaliana), the transcript encoding the blue copper protein PLANTACYANIN (PCY) is the primary target for miR408 in vegetative tissues. PCY is preferentially expressed
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Proteolysis in plant immunity Plant Cell (IF 10.0) Pub Date : 2024-05-09 Yanan Liu, Edan Jackson, Xueru Liu, Xingchuan Huang, Renier A L van der Hoorn, Yuelin Zhang, Xin Li
Compared with transcription and translation, protein degradation machineries can act faster and be targeted to different subcellular compartments, enabling immediate regulation of signaling events. It is therefore not surprising that proteolysis has been used extensively to control homeostasis of key regulators in different biological processes and pathways. Over the past decades, numerous studies
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A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis Plant Cell (IF 10.0) Pub Date : 2024-05-09 Julia Legen, Benjamin Lenzen, Nitin Kachariya, Stephanie Feltgen, Yang Gao, Simon Mergenthal, Willi Weber, Enrico Klotzsch, Reimo Zoschke, Michael Sattler, Christian Schmitz-Linneweber
Arabidopsis (Arabidopsis thaliana) plants can produce photosynthetic tissue with active chloroplasts at temperatures as low as 4°C, and this process depends on the presence of the nuclear-encoded, chloroplast-localized RNA-binding protein CP29A. In this study, we demonstrate that CP29A undergoes phase separation in vitro and in vivo in a temperature-dependent manner, which is mediated by a prion-like
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GROWTH REGULATING FACTOR 7–mediated arbutin metabolism enhances rice salt tolerance Plant Cell (IF 10.0) Pub Date : 2024-05-03 Yunping Chen, Zhiwu Dan, Shaoqing Li
Salt stress is an environmental factor that limits plant growth and crop production. With the rapid expansion of salinized arable land worldwide, investigating the molecular mechanisms underlying the salt stress response in plants is urgently needed. Here, we report that GROWTH REGULATING FACTOR 7 (OsGRF7) promotes salt tolerance by regulating arbutin (hydroquinone-β-D-glucopyranoside) metabolism in
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Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants Plant Cell (IF 10.0) Pub Date : 2024-05-03 Erika Isono, Jianming Li, Pablo Pulido, Wei Siao, Steven H Spoel, Zhishuo Wang, Xiaohong Zhuang, Marco Trujillo
Proteome composition is dynamic and influenced by many internal and external cues, including developmental signals, light availability, or environmental stresses. Protein degradation, in synergy with protein biosynthesis, allows cells to respond to various stimuli and adapt by reshaping the proteome. Protein degradation mediates the final and irreversible disassembly of proteins, which is important
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Perspectives on improving photosynthesis to increase crop yield Plant Cell (IF 10.0) Pub Date : 2024-05-03 Roberta Croce, Elizabete Carmo-Silva, Young B Cho, Maria Ermakova, Jeremy Harbinson, Tracy Lawson, Alistair J McCormick, Krishna K Niyogi, Donald R Ort, Dhruv Patel-Tupper, Paolo Pesaresi, Christine Raines, Andreas P M Weber, Xin-Guang Zhu
Improving photosynthesis, the fundamental process by which plants convert light energy into chemical energy, is a key area of research with great potential for enhancing sustainable agricultural productivity and addressing global food security challenges. This perspective delves into the latest advancements and approaches aimed at optimizing photosynthetic efficiency. Our discussion encompasses the
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Variation in WIDTH OF LEAF AND GRAIN contributes to grain and leaf size by controlling LARGE2 stability in rice Plant Cell (IF 10.0) Pub Date : 2024-05-03 Zhichuang Yue, Zhipeng Wang, Yilong Yao, Yuanlin Liang, Jiaying Li, Kaili Yin, Ruiying Li, Yibo Li, Yidan Ouyang, Lizhong Xiong, Honghong Hu
Grain and flag leaf size are two important agronomic traits that influence grain yield in rice (Oryza sativa). Many QTLs and genes that regulate these traits individually have been identified, however, few QTLs and genes that simultaneously control these two traits have been identified. In this study, we conducted a genome-wide association analysis in rice and detected a major locus, WIDTH OF LEAF
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Arabinosylation of cell wall extensin is required for the directional response to salinity in roots Plant Cell (IF 10.0) Pub Date : 2024-05-01 Yutao Zou, Nora Gigli-Bisceglia, Eva van Zelm, Pinelopi Kokkinopoulou, Magdalena M Julkowska, Maarten Besten, Thu-Phuong Nguyen, Hongfei Li, Jasper Lamers, Thijs de Zeeuw, Joram A Dongus, Yuxiao Zeng, Yu Cheng, Iko T Koevoets, Bodil Jørgensen, Marcel Giesbers, Jelmer Vroom, Tijs Ketelaar, Bent Larsen Petersen, Timo Engelsdorf, Joris Sprakel, Yanxia Zhang, Christa Testerink
Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in
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CAM evolution is associated with gene family expansion in an explosive bromeliad radiation Plant Cell (IF 10.0) Pub Date : 2024-04-30 Clara Groot Crego, Jaqueline Hess, Gil Yardeni, Marylaure de La Harpe, Clara Priemer, Francesca Beclin, Sarah Saadain, Luiz A Cauz-Santos, Eva M Temsch, Hanna Weiss-Schneeweiss, Michael H J Barfuss, Walter Till, Wolfram Weckwerth, Karolina Heyduk, Christian Lexer, Ovidiu Paun, Thibault Leroy
The subgenus Tillandsia (Bromeliaceae) belongs to one of the fastest radiating clades in the plant kingdom and is characterised by the repeated evolution of Crassulacean acid metabolism (CAM). Despite its complex genetic basis, this water-conserving trait has evolved independently across many plant families and is regarded as a key innovation trait and driver of ecological diversification in Bromeliaceae
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Photosynthetic control at the cytochrome b6f complex Plant Cell (IF 10.0) Pub Date : 2024-04-26 Gustaf E Degen, Matthew P Johnson
Photosynthetic control (PCON) is a protective mechanism that prevents light-induced damage to photosystem I (PSI) by ensuring the rate of NADPH and ATP production via linear electron transfer (LET) is balanced by their consumption in the CO2 fixation reactions. Protection of PSI is a priority for plants since they lack a dedicated rapid-repair cycle for this complex, meaning that any damage leads to
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Vacuolar Degradation of Plant Organelles Plant Cell (IF 10.0) Pub Date : 2024-04-24 Marisa S Otegui, Charlotte Steelheart, Wenlong Ma, Juncai Ma, Byung-Ho Kang, Victor Sanchez De Medina Hernandez, Yasin Dagdas, Caiji Gao, Shino Goto-Yamada, Kazusato Oikawa, Mikio Nishimura
Plants continuously remodel and degrade their organelles due to damage from their metabolic activities and environmental stressors, as well as an integral part of their cell differentiation programs. Whereas certain organelles use local hydrolytic enzymes for limited remodeling, most of pathways that control the partial or complete dismantling of organelles rely on vacuolar degradation. Specifically
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Cytochrome b5 diversity in green lineages preceded the evolution of syringyl lignin biosynthesis Plant Cell (IF 10.0) Pub Date : 2024-04-24 Xianhai Zhao, Yunjun Zhao, Qing-yin Zeng, Chang-Jun Liu
Lignin production marked a milestone in vascular plant evolution, and the emergence of syringyl (S)-lignin is lineage-specific. S-lignin biosynthesis in angiosperms, mediated by ferulate 5-hydroxylase (F5H, CYP84A1), has been considered a recent evolutionary event. F5H uniquely requires the cytochrome b5 protein CB5D as an obligatory redox partner for catalysis. However, it remains unclear how CB5D
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Erasing marks: Functions of plant deubiquitylating enzymes in modulating the ubiquitin code Plant Cell (IF 10.0) Pub Date : 2024-04-24 Karin Vogel, Erika Isono
Plant cells need to respond to environmental stimuli and developmental signals accurately and promptly. Ubiquitylation is a reversible posttranslational modification that enables the adaptation of cellular proteostasis to internal or external factors. The different topologies of ubiquitin linkages serve as the structural basis for the ubiquitin code, which can be interpreted by ubiquitin-binding proteins
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The structural basis for light harvesting in organisms producing phycobiliproteins Plant Cell (IF 10.0) Pub Date : 2024-04-23 Donald A Bryant, Christopher J Gisriel
Cyanobacteria, red algae, and cryptophytes produce two classes of proteins for light-harvesting: water-soluble phycobiliproteins and membrane-intrinsic proteins that bind chlorophylls and carotenoids. In cyanobacteria, red algae, and glaucophytes, phycobilisomes (PBS) are complexes of brightly colored phycobiliproteins and linker (assembly) proteins. To date, six structural classes of phycobilisomes
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The H3K4 demethylase JMJ1 is required for proper timing of flowering in Brachypodium distachyon Plant Cell (IF 10.0) Pub Date : 2024-04-23 Bing Liu, Chengzhang Li, Xiang Li, Jiachen Wang, Wenhao Xie, Daniel P Woods, Weiya Li, Xiaoyu Zhu, Shuoming Yang, Aiwu Dong, Richard M Amasino
Flowering is a key developmental transition in the plant life cycle. In temperate climates, flowering often occurs in response to the perception of seasonal cues such as changes in day-length and temperature. However, the mechanisms that have evolved to control the timing of flowering in temperate grasses are not fully understood. We identified a Brachypodium distachyon mutant whose flowering is delayed
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Protein degradation in the auxin response Plant Cell (IF 10.0) Pub Date : 2024-04-23 Martijn de Roij, Jan Willem Borst, Dolf Weijers
The signaling molecule auxin sits at the nexus of plant biology and coordinates essentially all growth and developmental processes in plants. Auxin molecules are transported throughout plant tissues and are capable of evoking highly specific physiological responses in plant cells by inducing various molecular pathways. In many of these pathways, proteolysis plays a crucial role for correct physiological
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Arabidopsis class A S-acyl transferases modify the pollen receptors LIP1 and PRK1 to regulate pollen tube guidance Plant Cell (IF 10.0) Pub Date : 2024-04-18 Xiaojiao Xiang, Zhiyuan Wan, Shuzhan Zhang, Qiang-Nan Feng, Shan-Wei Li, Gui-Min Yin, Jing-Yu Zhi, Xin Liang, Ting Ma, Sha Li, Yan Zhang
Protein S-acylation catalyzed by protein S-acyl transferases (PATs) is a reversible lipid modification regulating protein targeting, stability, and interaction profiles. PATs are encoded by large gene families in plants, and many proteins including receptor-like cytoplasmic kinases (RLCKs) and receptor-like kinases (RLKs) are subject to S-acylation. However, few PATs have been assigned substrates,
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The transcriptome landscape of developing barley seeds Plant Cell (IF 10.0) Pub Date : 2024-04-18 Martin Kovacik, Anna Nowicka, Jana Zwyrtková, Beáta Strejčková, Isaia Vardanega, Eddi Esteban, Asher Pasha, Kateřina Kaduchová, Maryna Krautsova, Marie Červenková, Jan Šafář, Nicholas J Provart, Rüdiger Simon, Ales Pecinka
Cereal grains are an important source of food and feed. To provide comprehensive spatiotemporal information about biological processes in developing seeds of cultivated barley (Hordeum vulgare L. subsp. vulgare), we performed a transcriptomic study of the embryo, endosperm, and seed maternal tissues collected from grains 4–32 days after pollination. Weighted gene co-expression network and motif enrichment
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The GRAS transcription factor CsTL regulates tendril formation in cucumber Plant Cell (IF 10.0) Pub Date : 2024-04-17 Junjun Shen, Yanxin Jiang, Jian Pan, Linhan Sun, Qingqing Li, Wenjing He, Piaoyun Sun, Bosi Zhao, Hongjiao Zhao, Xubo Ke, Yalu Guo, Tongwen Yang, Zheng Li
Cucumber (Cucumis sativus, Cs) tendrils are slender vegetative organs that typically require manual removal to ensure orderly growth during greenhouse cultivation. Here, we identified cucumber tendril-less (tl), a Tnt1 retrotransposon-induced insertion mutant lacking tendrils. Map-based cloning identified the mutated gene, CsaV3_3G003590, which we designated as CsTL, which is homologous to Arabidopsis
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BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition Plant Cell (IF 10.0) Pub Date : 2024-04-12 Hongtao Zhang, Chelsea Rundle, Nikola Winter, Alexandra Miricescu, Brian C Mooney, Andreas Bachmair, Emmanuelle Graciet, Frederica L Theodoulou
BIG/DARK OVEREXPRESSION OF CAB1/TRANSPORT INHIBITOR RESPONSE3 is a 0.5-MDa protein associated with multiple functions in Arabidopsis (Arabidopsis thaliana) signalling and development. However, the biochemical functions of BIG are unknown. We investigated a role for BIG in the Arg/N-degron pathways, in which substrate protein fate is influenced by the N-terminal (Nt) residue. We crossed a big loss-of-function
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The N-terminal domains of NLR immune receptors exhibit structural and functional similarities across divergent plant lineages Plant Cell (IF 10.0) Pub Date : 2024-04-10 Khong-Sam Chia, Jiorgos Kourelis, Albin Teulet, Martin Vickers, Toshiyuki Sakai, Joseph F Walker, Sebastian Schornack, Sophien Kamoun, Philip Carella
Nucleotide-binding domain and leucine-rich repeat (NLR) proteins are a prominent class of intracellular immune receptors in plants. However, our understanding of plant NLR structure and function is limited to the evolutionarily young flowering plant clade. Here, we describe an extended spectrum of NLR diversity across divergent plant lineages and demonstrate the structural and functional similarities
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The Y locus encodes a REPRESSOR OF PHOTOSYNTHETIC GENES protein that represses carotenoid biosynthesis via interaction with APRR2 in carrot Plant Cell (IF 10.0) Pub Date : 2024-04-09 Ying-Gang Wang, Yu-Min Zhang, Ya-Hui Wang, Kai Zhang, Jing Ma, Jia-Xin Hang, Yu-Ting Su, Shan-Shan Tan, Hui Liu, Ai-Sheng Xiong, Zhi-Sheng Xu
Little is known about the factors regulating carotenoid biosynthesis in roots. In this study, we characterized DCAR_032551, the candidate gene of the Y locus responsible for the transition of root color from ancestral white to yellow during carrot (Daucus carota) domestication. We show that DCAR_032551 encodes a REPRESSOR OF PHOTOSYNTHETIC GENES (RPGE) protein, named DcRPGE1. DcRPGE1 from wild carrot
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The phytochrome-interacting factor genes PIF1 and PIF4 are functionally diversified due to divergence of promoters and proteins Plant Cell (IF 10.0) Pub Date : 2024-04-09 Hanim Kim, Nayoung Lee, Yeojae Kim, Giltsu Choi
Phytochrome-interacting factors (PIFs) are basic helix-loop-helix transcription factors that regulate light responses downstream of phytochromes. In Arabidopsis (Arabidopsis thaliana), eight PIFs (PIF1-8) regulate light responses, either redundantly or distinctively. Distinctive roles of PIFs may be attributed to differences in mRNA expression patterns governed by promoters or variations in molecular