Generally, the metabolism in microbial organism is an intricate, spatiotemporal process that emerges from gene regulatory networks, which affects the efficiency of product biosynthesis. With the coming age of synthetic biology, spatiotemporal control systems have been explored as versatile strategies to promote product biosynthesis at both spatial and temporal levels. Meanwhile, the designer synthetic compartments provide new and promising approaches to engineerable spatiotemporal control systems to construct high-performance microbial cell factories. In this article, we comprehensively summarize recent developments in spatiotemporal control systems for tailoring advanced cell factories, and illustrate how to apply spatiotemporal control systems in different microbial species with desired applications. Future challenges of spatiotemporal control systems and perspectives are also discussed.

中文翻译：

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通过细胞代谢的多重时空控制来设计微生物细胞工厂：进展、挑战和未来展望


一般来说，微生物的代谢是一个错综复杂的时空过程，它出现在基因调控网络中，这会影响产物生物合成的效率。随着合成生物学时代的到来，时空控制系统已被探索为在空间和时间水平上促进产物生物合成的通用策略。同时，设计合成隔室为可工程的时空控制系统提供了新的和有前途的方法，以构建高性能微生物细胞工厂。在本文中，我们全面总结了用于定制高级细胞工厂的时空控制系统的最新发展，并说明了如何将时空控制系统应用于不同的微生物物种并具有所需的应用。还讨论了时空控制系统和视角的未来挑战。