Biomanufacturing, driven by technologies such as synthetic biology, offers significant potential to advance the bioeconomy and promote sustainable development. It is anticipated to transform traditional manufacturing and become a key industry in future strategies. Cell factories are the core of biomanufacturing. The advancement of synthetic biology and growing market demand have led to the production of a greater variety of natural products and increasingly complex metabolic pathways. However, this progress also presents challenges, notably the conflict between natural product production and chassis cell growth. This conflict results in low productivity and yield, adverse side effects, metabolic imbalances, and growth retardation. Enzyme co-localization strategies have emerged as a promising solution. This article reviews recent progress and applications of these strategies in constructing cell factories for efficient natural product production. It comprehensively describes the applications of enzyme-based compartmentalization, metabolic pathway-based compartmentalization, and synthetic organelle-based compartmentalization in improving product titers. The article also explores future research directions and the prospects of combining multiple strategies with advanced technologies.

中文翻译：

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微生物细胞工厂构建的多酶共定位策略研究进展


由合成生物学等技术驱动的生物制造为推动生物经济和促进可持续发展提供了巨大潜力。有望改造传统制造业，成为未来战略的重点产业。细胞工厂是生物制造的核心。合成生物学的进步和不断增长的市场需求导致了更多种类的天然产物的生产和日益复杂的代谢途径。然而，这一进展也带来了挑战，特别是自然产品生产和底盘细胞生长之间的冲突。这种冲突导致生产力和产量低下、不良副作用、代谢失衡和生长迟缓。酶共定位策略已成为一种有前途的解决方案。本文回顾了这些策略在构建细胞工厂以高效生产天然产物方面的最新进展和应用。它全面描述了基于酶的区室化、基于代谢途径的区室化和基于合成细胞器的区室化在提高产品效价方面的应用。文章还探讨了未来的研究方向以及多种策略与先进技术相结合的前景。