L-Phenylalanine (L-Phe) is an important aromatic amino acid, which has been widely used in food, health care products, medicine and other fields. Based on the relatively mature microbial biosynthesis process, a variety of -phenylalanine-derived compounds have attracted more and more attentions owing to their extensively potential applications in the fields of food, medicine, spices, cosmetics, and pesticides. However, the challenge of biosynthesis of -phenylalanine-derived compounds remains the issue of low production and productivity. With the development of metabolic engineering and synthetic biology, the biosynthesis of -phenylalanine has reached a high level. Therefore, the synthesis of -phenylalanine-derived compounds based on high production strains of -phenylalanine has broad prospects. In addition, some -phenylalanine-derived compounds are more suitable for efficient synthesis by exogenous addition of precursors due to their longer metabolic pathways and the inhibitory effects of many intermediate products. This review systematically summarized the research progress of -phenylalanine-derived compounds, including phenylpyruvate derivatives, -cinnamic derivatives, -coumaric acid derivatives and other -phenylalanine-derived compounds (such as flavonoids). Finally, the main strategies to improve the production of -phenylalanine-derived compounds were summarized, and the development trends of the synthesis of -phenylalanine-derived compounds by microbial method were also prospected.

中文翻译：

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工程微生物生物合成 L-苯丙氨酸衍生化合物


L-苯丙氨酸（L-Phe）是一种重要的芳香族氨基酸，已广泛应用于食品、保健品、医药等领域。基于相对成熟的微生物生物合成工艺，多种β-苯丙氨酸衍生化合物因其在食品、医药、香料、化妆品、农药等领域的广泛潜在应用而受到越来越多的关注。然而，β-苯丙氨酸衍生化合物的生物合成面临的挑战仍然是产量和生产率低的问题。随着代谢工程和合成生物学的发展，β-苯丙氨酸的生物合成已达到较高水平。因此，基于β-苯丙氨酸高产菌株合成β-苯丙氨酸衍生化合物具有广阔的前景。此外，一些β-苯丙氨酸衍生的化合物由于其较长的代谢途径和许多中间产物的抑制作用，更适合通过外源添加前体进行高效合成。本文系统综述了β-苯丙氨酸衍生化合物的研究进展，包括苯丙酮酸衍生物、β-肉桂酸衍生物、β-香豆酸衍生物以及其他β-苯丙氨酸衍生化合物（如黄酮类化合物）。最后总结了提高β-苯丙氨酸衍生物产量的主要策略，并对微生物法合成β-苯丙氨酸衍生物的发展趋势进行了展望。