Enabling the transition towards a future circular bioeconomy based on industrial biomanufacturing necessitates the development of efficient and versatile microbial platforms for sustainable chemical and fuel production. Recently, there has been growing interest in engineering non-model microbes as superior biomanufacturing platforms due to their broad substrate range and high resistance to stress conditions. Among these non-conventional microbes, red yeasts belonging to the genus have emerged as promising industrial chassis for the production of specialty chemicals such as oleochemicals, organic acids, fatty acid derivatives, terpenoids, and other valuable compounds. Advancements in genetic and metabolic engineering techniques, coupled with systems biology analysis, have significantly enhanced the production capacity of red yeasts. These developments have also expanded the range of substrates and products that can be utilized or synthesized by these yeast species. This review comprehensively examines the current efforts and recent progress made in red yeast research. It encompasses the exploration of available substrates, systems analysis using multi-omics data, establishment of genome-scale models, development of efficient molecular tools, identification of genetic elements, and engineering approaches for the production of various industrially relevant bioproducts. Furthermore, strategies to improve substrate conversion and product formation both with systematic and synthetic biology approaches are discussed, along with future directions and perspectives in improving red yeasts as more versatile biotechnological chassis in contributing to a circular bioeconomy. The review aims to provide insights and directions for further research in this rapidly evolving field. Ultimately, harnessing the capabilities of red yeasts will play a crucial role in paving the way towards next-generation sustainable bioeconomy.

中文翻译：

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将含油红酵母设计为生产油脂化学品和有价值化合物的多功能底盘：当前进展和前景


要实现向基于工业生物制造的未来循环生物经济的过渡，就必须开发高效且多功能的微生物平台，以实现可持续的化学品和燃料生产。最近，由于非模型微生物具有广泛的底物范围和对应激条件的高抵抗力，人们对工程化非模型微生物作为卓越的生物制造平台越来越感兴趣。在这些非常规微生物中，属于该属的红酵母已成为生产特种化学品（如油脂化学品、有机酸、脂肪酸衍生物、萜类化合物和其他有价值的化合物）的有前途的工业基础。遗传和代谢工程技术的进步，加上系统生物学分析，显着提高了红酵母的生产能力。这些发展还扩大了这些酵母物种可以利用或合成的底物和产品的范围。这篇综述全面考察了红曲研究目前的努力和最新进展。它包括探索可用底物、使用多组学数据进行系统分析、建立基因组规模模型、开发高效分子工具、识别遗传元件以及生产各种工业相关生物产品的工程方法。此外，还讨论了通过系统生物学和合成生物学方法改善底物转化和产物形成的策略，以及改进红酵母作为更通用的生物技术底盘以促进循环生物经济的未来方向和前景。该综述旨在为这个快速发展的领域的进一步研究提供见解和方向。 最终，利用红酵母的能力将在为下一代可持续生物经济铺平道路方面发挥至关重要的作用。