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PhieDBEs: a DBD‐containing, PAM‐flexible, high‐efficiency dual base editor toolbox with wide targeting scope for use in plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-19 Zhiye Zheng, Taoli Liu, Nan Chai, Dongchang Zeng, Ruixiang Zhang, Yang Wu, Jiaxuan Hang, Yuxin Liu, Qindi Deng, Jiantao Tan, Jialin Liu, Xianrong Xie, Yao‐Guang Liu, Qinlong Zhu
SummaryDual base editors (DBEs) enable simultaneous A‐to‐G and C‐to‐T conversions, expanding mutation types. However, low editing efficiency and narrow targeting range limit the widespread use of DBEs in plants. The single‐strand DNA binding domain of RAD51 DBD can be fused to base editors to improve their editing efficiency. However, it remains unclear how the DBD affects dual base editing performance
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Polyamines: pleiotropic molecules regulating plant development and enhancing crop yield and quality Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-18 Haishan Yang, Yinyin Fang, Zhiman Liang, Tian Qin, Ji‐Hong Liu, Taibo Liu
SummaryPolyamines (PAs) are pleiotropic bioorganic molecules. Cellular PA contents are determined by a balance between PA synthesis and degradation. PAs have been extensively demonstrated to play vital roles in the modulation of plant developmental processes and adaptation to various environmental stresses. In this review, the latest advances on the diverse roles of PAs in a range of developmental
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Ectopic enhancer–enhancer interactions as causal forces driving RNA‐directed DNA methylation in gene regulatory regions Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-18 Yazhou Yang, Jia Liu, Stacy D. Singer, Guohua Yan, Dennis R. Bennet, Yue Liu, Jean‐Michel Hily, Weirong Xu, Yingzhen Yang, Xiping Wang, Gan‐Yuan Zhong, Zhongchi Liu, Yong‐Chiang An, Huawei Liu, Zongrang Liu
SummaryCis‐regulatory elements (CREs) are integral to the spatiotemporal and quantitative expression dynamics of target genes, thus directly influencing phenotypic variation and evolution. However, many of these CREs become highly susceptible to transcriptional silencing when in a transgenic state, particularly when organised as tandem repeats. We investigated the mechanism of this phenomenon and found
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Genetic variation in the aquaporin TONOPLAST INTRINSIC PROTEIN 4;3 modulates maize cold tolerance Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-18 Rong Zeng, Xiaoyan Zhang, Guangshu Song, Qingxue Lv, Minze Li, Diyi Fu, Zhuo Zhang, Lei Gao, Shuaisong Zhang, Xiaohong Yang, Feng Tian, Shuhua Yang, Yiting Shi
SummaryCold stress is a major abiotic stress that threatens maize (Zea mays L.) production worldwide. Understanding the molecular mechanisms underlying cold tolerance is crucial for breeding resilient maize varieties. Tonoplast intrinsic proteins (TIPs) are a subfamily of aquaporins in plants. Here, we report that TIP family proteins are involved in maize cold tolerance. The expression of most TIP
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Enhanced efficacy of glycoengineered rice cell‐produced trastuzumab Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-17 Jun‐Hye Shin, Sera Oh, Mi‐Hwa Jang, Seok‐Yong Lee, Chanhong Min, Young‐Jae Eu, Hilal Begum, Jong‐Chan Kim, Gap Ryol Lee, Han‐Bin Oh, Matthew J. Paul, Julian K.‐C. Ma, Ho‐Shin Gwak, Hyewon Youn, Seong‐Ryong Kim
SummaryFor several decades, a plant‐based expression system has been proposed as an alternative platform for the production of biopharmaceuticals including therapeutic monoclonal antibodies (mAbs), but the immunogenicity concerns associated with plant‐specific N‐glycans attached in plant‐based biopharmaceuticals has not been completely solved. To eliminate all plant‐specific N‐glycan structure, eight
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Knockout of ZmNST2 promotes bioethanol production from corn stover Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-15 Ying Wang, Ye Xing, Xinyu Yang, Yanwen Yu, Jiankun Li, Chenyang Zhao, Mengyu Yuan, Weili Huang, Yue Yin, Guohui Liu, Yuqing Sun, Haochuan Li, Jihua Tang, Qin Zhang, Mingyue Gou
The crude oil crisis causes an increasing demand of renewable energy, among which, bioethanol is considered the cleanest and renewable liquid fuel alternative to fossil fuel (An Tran et al., 2019). Bioethanol was mostly produced from sugarcane and corn, which violates vigorously against the world's food security. Alternatively, efforts have been made to produce bioethanol from non-food lignocellulose
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Development of a multi‐resistance and high‐yield rice variety using multigene transformation and gene editing Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-14 Changyan Li, Zaihui Zhou, Xinzhu Xiong, Chuanxu Li, Chuanhong Li, Enlong Shen, Jianyu Wang, Wenjun Zha, Bian Wu, Hao Chen, Lei Zhou, Yongjun Lin, Aiqing You
Approximately one-third of the total annual food production in the world is lost owing to pests, diseases and weeds. Therefore, the challenges posed by crop losses and population growth have emphasized the need for better breeding techniques (FAO et al., 2023). Practical experience has demonstrated that the utilization of existing resistance genes to breed and cultivate herbicide- and pest-resistant
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Precision editing of a susceptibility gene promoter to alter its methylation modification for engineering rice resilience to biotic and abiotic stresses Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-14 Jingjing Tian, Hang Zhang, Shuxin Li, Yongjun Lin, Lizhong Xiong, Meng Yuan
Rice is a primary food crop, and its yield is threatened by biotic and abiotic stresses. Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a chief bacterial disease of rice. Xoo infects rice depending on its transcriptional activation-like effectors (TALEs), which specifically target effector binding elements (EBEs) in the promoter of host susceptibility (S) genes and regulate S genes' expression
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Cotton BOP1 mediates SUMOylation of GhBES1 to regulate fibre development and plant architecture Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-14 Bingting Wang, Zhian Wang, Ye Tang, Naiqin Zhong, Jiahe Wu
SummaryThe Arabidopsis BLADE‐ON‐PETIOLE (BOP) genes are primarily known for their roles in regulating leaf and floral patterning. However, the broader functions of BOPs in regulating plant traits remain largely unexplored. In this study, we investigated the role of the Gossypium hirsutum BOP1 gene in the regulation of fibre length and plant height through the brassinosteroid (BR) signalling pathway
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CRISPR/Cas9‐mediated editing of Bs5 and Bs5L in tomato leads to resistance against Xanthomonas Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-13 Arturo Ortega, Kyungyong Seong, Alex Schultink, Daniela Paula de Toledo Thomazella, Eunyoung Seo, Elaine Zhang, Julie Pham, Myeong‐Je Cho, Douglas Dahlbeck, Jacqueline Warren, Gerald V. Minsavage, Jeffrey B. Jones, Edgar Sierra‐Orozco, Samuel F. Hutton, Brian Staskawicz
Bacterial spot, caused by Xanthomonas species, is a devastating disease of tomato (Solanum lycopersicum) and pepper (Capsicum annuum) (Schwartz et al., 2015). The recessively inherited resistance, bacterial spot 5 (bs5), in pepper (hereafter referred to as Cabs5) can confer resistance against different Xanthomonas strains (Jones et al., 2002). The Cabs5 resistance is characterized by the absence of
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Just FIND‐IT: Harnessing the true power of induced mutagenesis Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-09 Christoph Dockter, Søren Knudsen, Magnus Wohlfahrt Rasmussen, Birgitte Skadhauge, Birger Lindberg Møller
In nature, genetic variation occurs in every population and results in the evolution of a diversity of new properties, some of which promote the survival of the species. To accelerate nature's evolution based on genetic diversity, plant breeders may induce additional mutations to raise the number of genetic variations increasing the chances to obtain varieties with new desired traits like improved
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Bt corn and cotton planting may benefit peanut growers by reducing aflatoxin risk Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-08 Jina Yu, David A. Hennessy, Felicia Wu
SummaryDecades of studies have shown that Bt corn, by reducing insect damage, has lower levels of mycotoxins (fungal toxins), such as aflatoxin and fumonisin, than conventional corn. We used crop insurance data to infer that this benefit from Bt crops extends to reducing aflatoxin risk in peanuts: a non‐Bt crop. In consequence, we suggest that any benefit–cost assessment of how transgenic Bt crops
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Fine‐tuning the N‐glycosylation of recombinant human erythropoietin using Chlamydomonas reinhardtii mutants Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-05 S. Leprovost, C. Plasson, J. Balieu, M‐L. Walet‐Balieu, P. Lerouge, M. Bardor, E. Mathieu‐Rivet
SummaryMicroalgae are considered as attractive expression systems for the production of biologics. As photosynthetic unicellular organisms, they do not require costly and complex media for growing and are able to secrete proteins and perform protein glycosylation. Some biologics have been successfully produced in the green microalgae Chlamydomonas reinhardtii. However, post‐translational modifications
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Mutation of the Vinv 5′ UTR regulatory region reduces acrylamide levels in processed potato to reach EU food‐safety standards Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-02 Leonard Shumbe, Emanoella Soares, Yordan Muhovski, Inga Smit, Hervé Vanderschuren
The recent prohibition of Chlorpropham (CIPC) in the EU (Commission Implementing Regulation(EU) 2019/989) is prompting the potato processing industry to search for alternative and safer anti-sprouting approaches. Storage at cold temperature (i.e. 4 °C) has emerged as a valuable option for long term storage of potato without the use of CIPC. However, most commercial potato varieties accumulate high
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Enhancing peanut nutritional quality by editing AhKCS genes lacking natural variation Plant Biotech. J. (IF 10.1) Pub Date : 2024-07-01 Dongxin Huai, Xiaomeng Xue, Jie Wu, Manish K. Pandey, Nian Liu, Li Huang, Liying Yan, Yuning Chen, Xin Wang, Qianqian Wang, Yanping Kang, Zhihui Wang, Huifang Jiang, Rajeev K. Varshney, Boshou Liao, Yong Lei
Peanut (Arachis hypogaea L.) is a globally staple oilseed crop, extensively cultivated in tropical and subtropical regions. Due to its substantial oil (approximately 46%–58%) and protein (around 22%–32%) content, the peanut plays a pivotal role in addressing malnutrition and ensuring food security in many regions. The fatty acid profiles of vegetable oil and foods have recently garnered increased attention
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Natural variation of WBR7 confers rice high yield and quality by modulating sucrose supply in sink organs Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-29 Huan Shi, Peng Yun, Yun Zhu, Lu Wang, Yipei Wang, Pingbo Li, Hao Zhou, Shiyuan Cheng, Rongjia Liu, Guanjun Gao, Qinglu Zhang, Jinghua Xiao, Yibo Li, Lizhong Xiong, Aiqing You, Yuqing He
SummaryGrain chalkiness is an undesirable trait that negatively regulates grain yield and quality in rice. However, the regulatory mechanism underlying chalkiness is complex and remains unclear. We identified a positive regulator of white‐belly rate (WBR). The WBR7 gene encodes sucrose synthase 3 (SUS3). A weak functional allele of WBR7 is beneficial in increasing grain yield and quality. During the
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A miniature alternative to Cas9 and Cas12: Transposon‐associated TnpB mediates targeted genome editing in plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-28 Subhasis Karmakar, Debasmita Panda, Sonali Panda, Manaswini Dash, Romio Saha, Priya Das, S.P. Avinash, Justin Shih, Yinong Yang, A. K. Nayak, Mirza J. Baig, Kutubuddin A. Molla
The two popular genome editor nucleases, Cas9 and Cas12a, hypothetically evolved from IscB and TnpB, respectively (Altae-Tran et al., 2021). Recent reports showed that TnpBs also function as RNA-guided nucleases in human cells (Karvelis et al., 2021). TnpB proteins are much smaller (~400 aa) than Cas9 (~1000–1400 aa) and Cas12a (~1300 aa). The large cargo size of Cas9 and Cas12a hinders their delivery
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The fnr‐like mutants confer isoxaben tolerance by initiating mitochondrial retrograde signalling Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-27 Ronan C. Broad, Michael Ogden, Arka Dutta, Peter M. Dracatos, James Whelan, Staffan Persson, Ghazanfar Abbas Khan
SummaryIsoxaben is a pre‐emergent herbicide used to control broadleaf weeds. While the phytotoxic mechanism is not completely understood, isoxaben interferes with cellulose synthesis. Certain mutations in cellulose synthase complex proteins can confer isoxaben tolerance; however, these mutations can cause compromised cellulose synthesis and perturbed plant growth, rendering them unsuitable as herbicide
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Delineation of loci governing an extra-earliness trait in lentil (Lens culinaris Medik.) using the QTL-Seq approach Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-25 Kumbarahally Murthigowda Shivaprasad, Harsh K. Dikshit, Gyan Prakash Mishra, Subodh Kumar Sinha, Muraleedhar Aski, Manju Kohli, Dwijesh C. Mishra, Amit Kumar Singh, Soma Gupta, Akanksha Singh, Kuldeep Tripathi, Ranjeet Ranjan Kumar, Atul Kumar, Girish Kumar Jha, Shiv Kumar, Rajeev K. Varshney
Developing early maturing lentil has the potential to minimize yield losses, mainly during terminal drought. Whole-genome resequencing (WGRS) based QTL-seq identified the loci governing earliness in lentil. The genetic analysis for maturity duration provided a good fit to 3:1 segregation (F2), indicating earliness as a recessive trait. WGRS of Globe Mutant (late parent), late-flowering, and early-flowering
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A CRISPR/Cas9‐mediated mutant library of seed‐preferred genes in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-25 Dongsheng Zhao, Siyu Chen, Yangshuo Han, Guanqing Liu, Jinyu Liu, Qingqing Yang, Tao Zhang, Jilei Shen, Xiaolei Fan, Changquan Zhang, Tao Zhang, Qianfeng Li, Chen Chen, Qiaoquan Liu
Rice seeds are an important energy source for humans. Seed traits are difficult to observe and controlled by complex networks. Therefore, mutant libraries enriched in seed traits are vital for interpreting gene functions during seed development as well as grain yield and quality formation. Using the simple and efficient genomic editing tool, several CRISPR/Cas9-based mutant libraries have been generated
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The complex hexaploid oil‐Camellia genome traces back its phylogenomic history and multi‐omics analysis of Camellia oil biosynthesis Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-24 Huaguo Zhu, Fuqiu Wang, Zhongping Xu, Guanying Wang, Lisong Hu, Junyong Cheng, Xianhong Ge, Jinxuan Liu, Wei Chen, Qiang Li, Fei Xue, Feng Liu, Wenying Li, Lan Wu, Xinqi Cheng, Xinxin Tang, Chaochen Yang, Keith Lindsey, Xianlong Zhang, Fang Ding, Haiyan Hu, Xiaoming Hu, Shuangxia Jin
SummaryOil‐Camellia (Camellia oleifera), belonging to the Theaceae family Camellia, is an important woody edible oil tree species. The Camellia oil in its mature seed kernels, mainly consists of more than 90% unsaturated fatty acids, tea polyphenols, flavonoids, squalene and other active substances, which is one of the best quality edible vegetable oils in the world. However, genetic research and molecular
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The superior allele LEA12OR in wild rice enhances salt tolerance and yield Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-24 Yuwei Ge, Gaoming Chen, Xinran Cheng, Chao Li, Yunlu Tian, Wenchao Chi, Jin Li, Zhaoyang Dai, Chunyuan Wang, Erchao Duan, Yan Liu, Zhiguang Sun, Jingfang Li, Baoxiang Wang, Dayong Xu, Xianjun Sun, Hui Zhang, Wenhua Zhang, Chunming Wang, Jianmin Wan
SummarySoil salinity has negative impacts on food security and sustainable agriculture. Ion homeostasis, osmotic adjustment and reactive oxygen species scavenging are the main approaches utilized by rice to resist salt stress. Breeding rice cultivars with high salt tolerance (ST) and yield is a significant challenge due to the lack of elite alleles conferring ST. Here, we report that the elite allele
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Correction Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-24
Correction to ‘Overexpression of Arabidopsis and Rice stress genes' inducible transcription factor confers drought and salinity tolerance to rice’ K. Datta, N. Baisakh, M. Ganguly, S. Krishnan, K. Yamaguchi Shinozai, S.K. Datta, “Overexpression of Arabidopsis and Rice stress genes' inducible transcription factor confers drought and salinity tolerance to rice,” Plant Biotechnology Journal 10, no. 5
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Trans‐complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-24 Weikuo Huang, Yuman Zhang, Na Xiao, Wenhui Zhao, Ying Shi, Rongxiang Fang
SummaryThe development of plant virus‐based expression systems has expanded rapidly owing to their potential applications in gene functional and disease resistance research, and industrial production of pharmaceutical proteins. However, the low yield of certain proteins, especially high‐molecular‐mass proteins, restricts the production scale. In this study, we observed that the tobacco mosaic virus
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Regulation of a single inositol 1‐phosphate synthase homeologue by HSFA6B contributes to fibre yield maintenance under drought conditions in upland cotton Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-22 Li'ang Yu, Anna C. Nelson Dittrich, Xiaodan Zhang, Jordan R. Brock, Venkatesh P. Thirumalaikumar, Giovanni Melandri, Aleksandra Skirycz, Patrick P. Edger, Kelly R. Thorp, Lori Hinze, Duke Pauli, Andrew D.L. Nelson
SummaryDrought stress substantially impacts crop physiology resulting in alteration of growth and productivity. Understanding the genetic and molecular crosstalk between stress responses and agronomically important traits such as fibre yield is particularly complicated in the allopolyploid species, upland cotton (Gossypium hirsutum), due to reduced sequence variability between A and D subgenomes. To
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Dendrobium thyrsiflorum genome and its molecular insights into genes involved in important horticultural traits Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-20 BingJie Chen, Jie‐Yu Wang, Pei‐Ji Zheng, Kang‐Li Li, Yu‐Min Liang, Xiao‐Fang Chen, Cuili Zhang, Xuewei Zhao, Xin He, Guo‐Qiang Zhang, Zhong‐Jian Liu, Qing Xu
Orchids possess significant ornamental value and are highly esteemed, especially among horticulturists. Numerous Dendrobium species are cultivated extensively due to their substantial commercial importance and the vast diversity in flower color and morphology (Teixeira da Silva et al., 2014). Dendrobium thyrsiflorum, characterized by its white flowers, yellow lips and globular inflorescences (Figure 1a)
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Integrating GWAS, RNA‐Seq and functional analysis revealed that BnaA02.SE mediates silique elongation by affecting cell proliferation and expansion in Brassica napus Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-20 Liyuan Zhang, Bo Yang, Xiaodong Li, Si Chen, Chao Zhang, Sirou Xiang, Tingting Sun, Ziyan Yang, Xizeng Kong, Cunmin Qu, Kun Lu, Jiana Li
SummaryRapeseed (Brassica napus) silique is the major carbohydrate source for seed development, and the final silique length has attracted great attention from breeders. However, no studies had focused on the dynamic character of silique elongation length (SEL). Here, the dynamic SEL investigation in a natural population including 588 lines over two years indicate that dynamic SEL during 0–20 days
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Production of grains with ultra‐low heavy metal accumulation by pyramiding novel Alleles of OsNramp5 and OsLsi2 in two‐line hybrid rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-20 Shikai Hu, Liang Zhou, Jingxin Wang, Amos Musyoki Mawia, Suozhen Hui, Bo Xu, Guiai Jiao, Zhonghua Sheng, Gaoneng Shao, Xiangjin Wei, Ling Wang, Lihong Xie, Fengli Zhao, Shaoqing Tang, Peisong Hu
SummaryEnsuring rice yield and grain safety quality are vital for human health. In this study, we developed two‐line hybrid rice (TLHR) with ultra‐low grain cadmium (Cd) and arsenic (As) accumulation by pyramiding novel alleles of OsNramp5 and OsLsi2. We first generated low Cd accumulation restorer (R) lines by editing OsNramp5, OsLCD, and OsLCT in japonica and indica. After confirming that OsNramp5
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The role of sugar transporters in the battle for carbon between plants and pathogens Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-16 Yi Chen, Anthony J. Miller, Bowen Qiu, Yao Huang, Kai Zhang, Gaili Fan, Xiaokun Liu
In photosynthetic cells, plants convert carbon dioxide to sugars that can be moved between cellular compartments by transporters before being subsequently metabolized to support plant growth and development. Most pathogens cannot synthesize sugars directly but have evolved mechanisms to obtain plant-derived sugars as C resource for successful infection and colonization. The availability of sugars to
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Epistasis and pleiotropy-induced variation for plant breeding Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-14 Sangam L. Dwivedi, Pat Heslop-Harrison, Junrey Amas, Rodomiro Ortiz, David Edwards
Epistasis refers to nonallelic interaction between genes that cause bias in estimates of genetic parameters for a phenotype with interactions of two or more genes affecting the same trait. Partitioning of epistatic effects allows true estimation of the genetic parameters affecting phenotypes. Multigenic variation plays a central role in the evolution of complex characteristics, among which pleiotropy
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Deciphering the mechanism of E3 ubiquitin ligases in plant responses to abiotic and biotic stresses and perspectives on PROTACs for crop resistance Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-12 Yingying Su, Guillaume Legrand Ngolong Ngea, Kaili Wang, Yuchun Lu, Esa Abiso Godana, Michael Ackah, Qiya Yang, Hongyin Zhang
With global climate change, it is essential to find strategies to make crops more resistant to different stresses and guarantee food security worldwide. E3 ubiquitin ligases are critical regulatory elements that are gaining importance due to their role in selecting proteins for degradation in the ubiquitin-proteasome proteolysis pathway. The role of E3 Ub ligases has been demonstrated in numerous cellular
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Soybean hypocotyl elongation is regulated by a MYB33-SWEET11/21-GA2ox8c module involving long-distance sucrose transport Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-11 Tong Su, Huan Liu, Yichun Wu, Jianhao Wang, Fanglei He, Haiyang Li, Shichen Li, Lingshuang Wang, Lanxin Li, Jie Cao, Qiulian Lu, Xiaohui Zhao, Hongtao Xiang, Chun Lin, Sijia Lu, Baohui Liu, Fanjiang Kong, Chao Fang
The length of hypocotyl affects the height of soybean and lodging resistance, thus determining the final grain yield. However, research on soybean hypocotyl length is scarce, and the regulatory mechanisms are not fully understood. Here, we identified a module controlling the transport of sucrose, where sucrose acts as a messenger moved from cotyledon to hypocotyl, regulating hypocotyl elongation. This
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Creating yellow seed Camelina sativa with enhanced oil accumulation by CRISPR-mediated disruption of Transparent Testa 8 Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-10 Yuanheng Cai, Yuanxue Liang, Hai Shi, Jodie Cui, Shreyas Prakash, Jianhui Zhang, Sanket Anaokar, Jin Chai, Jorg Schwender, Chaofu Lu, Xiao-Hong Yu, John Shanklin
Camelina (Camelina sativa L.), a hexaploid member of the Brassicaceae family, is an emerging oilseed crop being developed to meet the increasing demand for plant oils as biofuel feedstocks. In other Brassicas, high oil content can be associated with a yellow seed phenotype, which is unknown for camelina. We sought to create yellow seed camelina using CRISPR/Cas9 technology to disrupt its Transparent
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MaHsf24, a novel negative modulator, regulates cold tolerance in banana fruits by repressing the expression of HSPs and antioxidant enzyme genes Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-10 Jia Si, Zhong-qi Fan, Chao-jie Wu, Ying-ying Yang, Wei Shan, Jian-fei Kuang, Wang-jin Lu, Wei Wei, Jian-ye Chen
Transcriptional regulation mechanisms underlying chilling injury (CI) development have been widely investigated in model plants and cold-sensitive fruits, such as banana (Musa acuminata). However, unlike the well-known NAC and WRKY transcription factors (TFs), the function and deciphering mechanism of heat shock factors (HSFs) involving in cold response are still fragmented. Here, we showed that hot
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SBT5.2s are the major active extracellular subtilases processing IgG antibody 2F5 in the Nicotiana benthamiana apoplast Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-09 Konstantina Beritza, Pierre Buscaill, Shi-Jian Song, Philippe V. Jutras, Jie Huang, Lukas Mach, Suomeng Dong, Renier A. L. van der Hoorn
Agroinfiltration of Nicotiana benthamiana has emerged as a main protein expression platform in plant science and molecular pharming but yields are often hampered by endogenous proteases degrading recombinant proteins. Many IgG antibodies, for instance, are degraded when expressed in N. benthamiana (Niemer et al., 2014). HIV-neutralizing IgG antibody 2F5 is cleaved in the H3 loop of the variable region
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The miR7125-MdARF1 module enhances the resistance of apple to Colletotrichum gloeosporioides by promoting lignin synthesis in response to salicylic acid signalling Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-09 Xinmei Liu, Hongyan Dai, Feng Zhang, Jun Wang, Jiajun Shi, Junqin Chen, Ping He, Feng Wang, Yue Ma
Apple is an important cash crop in China, and it is susceptible to fungal infections that have deleterious effects on its yield. Apple bitter rot caused by Colletorichum gloeosporioides is one of the most severe fungal diseases of apple. Salicylic acid (SA) is a key signalling molecule in the plant disease resistance signalling pathways. Lignin synthesis also plays a key role in conferring disease
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Biochemical and nanotechnological approaches to combat phytoparasitic nematodes Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-03 Patrick Opdensteinen, Raghavan Charudattan, Jason C. Hong, Erin N. Rosskopf, Nicole F. Steinmetz
The foundation of most food production systems underpinning global food security is the careful management of soil resources. Embedded in the concept of soil health is the impact of diverse soil-borne pests and pathogens, and phytoparasitic nematodes represent a particular challenge. Root-knot nematodes and cyst nematodes are severe threats to agriculture, accounting for annual yield losses of US$157
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Coevolution unveiled: Sulfate transporters mediate rice resistance and susceptibility to Xanthomonas oryzae pv. oryzicola Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-03 Muhammad Sohaib Shafique, Liu Yapei, Li Man, Wang Hongjie, Su Ruyi, Wang Chunlian, Ji Zhiyuan
Rice (Oryza sativa L.) is an important cereal crop, feeding more than half of the world's population. The pathogen Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak (BLS) in rice and poses an emerging threat to food security (Liu et al., 2014). Genetic resistance is an efficient and sustainable solution to control diseases. Resistance resources against Xoc are scarce and largely unexplored
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A Syringa pinnatifolia genome assembly reveals the zerumbone biosynthesis machinery with a cytochrome P450-catalysed epoxidation reaction in eudicots Plant Biotech. J. (IF 10.1) Pub Date : 2024-06-02 Jiaqi Gao, Jing Wang, Ruishan Wang, Jian Wang, Ying Ma, Jian Yang, Badalahu Tai, Chongjiu Li, Xingyun Chai, Shungang Jiao, Tong Chen, Han Zheng, Xiang Li, Liping Kang, Chao Jiang, Junhui Zhou, Juan Liu, Luqi Huang
Syringa pinnatifolia, a shrub within the Syringa genus of the Oleaceae family (Figure S1), is valued in traditional Chinese medicine for its volatile chemical constituents. These principally include a suite of humulane-type monocyclic sesquiterpenoids with an 11-membered ring, notably zerumbone and its derivatives. Renowned for their cardioprotective, immunomodulatory and anti-carcinogenic properties
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Multiomics and biotechnologies for understanding and influencing cadmium accumulation and stress response in plants Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-31 Yan Yu, Saleh Alseekh, Zonghe Zhu, Kejin Zhou, Alisdair R. Fernie
SummaryCadmium (Cd) is one of the most toxic heavy metals faced by plants and, additionally, via the food chain, threatens human health. It is principally dispersed through agro‐ecosystems via anthropogenic activities and geogenic sources. Given its high mobility and persistence, Cd, although not required, can be readily assimilated by plants thereby posing a threat to plant growth and productivity
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CmBr confers fruit bitterness under CPPU treatment in melon Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-31 Mingyan Wang, Naiyu Jiang, Yuanchao Xu, Xinxiu Chen, Cui Wang, Chuangjiang Wang, Shiqi Wang, Kuipeng Xu, Sen Chai, Qing Yu, Zhonghua Zhang, Huimin Zhang
SummaryMany biotic or abiotic factors such as CPPU (N‐(2‐chloro‐pyridin‐4‐yl)‐N′‐phenylurea), a growth regulator of numerous crops, can induce bitterness in cucurbits. In melon, cucurbitacin B is the major compound leading to bitterness. However, the molecular mechanism underlying CuB biosynthesis in response to different conditions remains unclear. Here, we identified a set of genes involved in CPPU‐induced
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Coordinated control for the auricle asymmetric development by ZmIDD14 and ZmIDD15 fine‐tune the high‐density planting adaption in maize Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-31 Dan Liu, Qiang Ning, Lihong Zhai, Feng Teng, Yunfu Li, Ran Zhao, Qing Xiong, Jimin Zhan, Zhen Li, Fang Yang, Zuxin Zhang, Lei Liu
SummaryMultiple distinct specialized regions shape the architecture of maize leaves. Among them, the fringe‐like and wedge‐shaped auricles alter the angle between the leaf and stalk, which is a key trait in crop plant architecture. As planting density increased, a small leaf angle (LA) was typically selected to promote crop light capture efficiency and yield. In the present study, we characterized
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Correction Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-28
Correction for article ‘Rice-produced classical swine fever virus glycoprotein E2 with herringbone-dimer design to enhance immune responses’ Xu, Q., Ma, F., Yang, D., Li, Q., Yan, L., Ou, J., Zhang, L. et al. (2023) Rice-produced classical swine fever virus glycoprotein E2 with herringbone-dimer design to enhance immune responses. Plant Biotechnol. J., 21, 2546–2559. The authors want to rearrange the
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DeepCCR: large‐scale genomics‐based deep learning method for improving rice breeding Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-28 Xiaoding Ma, Hao Wang, Shengyang Wu, Bing Han, Di Cui, Jin Liu, Qiang Zhang, Xiuzhong Xia, Peng Song, Cuifeng Tang, Leiyue Geng, Yaolong Yang, Shen Yan, Kunneng Zhou, Longzhi Han
Rice is the staple food crop for half of the world's population. Traditional phenotype-based and marker-assisted selection methods have been used in rice improvement, but they are time-consuming, costly and labor-intensive. Therefore, research and implementation of novel breeding strategies to improve rice yield is a high priority. Genomic selection (GS) has paved the way for overcoming these limitations
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Seed plastids: A novel platform for recombinant protein expression Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-28 Malihe Mirzaee, Alyssa Leung, Mugdha Parulekar, Ana Candia, Aki Matsuoka, Kerry A. Lutz, Pal Maliga
Platforms for expression of recombinant proteins in plants are protein bodies in the seed (Kanai et al., 2023), and plant viral vectors and chloroplasts in leaves (Abrahamian et al., 2020; Bock, 2015; Maliga, 2021). Expression of recombinant proteins in chloroplasts has the advantages of high-level protein accumulation, expression of multiple genes in operons and natural biocontainment due to the lack
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Multiplex‐genome‐editing based rapid directional improvement of complex traits in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-28 Yidong Wei, Haomin Zhang, Jiaxing Fan, Qiuhua Cai, Zhixing Zhang, Jinlan Wang, Min Zhang, Fengting Yan, Jiahuan Jiang, Hongguang Xie, Xi Luo, Linyan Wei, Yuelong Lin, Wei He, Mengyu Qu, Xinxin Zhang, Yongsheng Zhu, Huaan Xie, Jianfu Zhang
SummaryAlthough thousands of genes have been identified or cloned in rice (Oryza sativa) in the last two decades, the majority of them have only been separately characterized in specific varieties or single‐gene modified backgrounds, thus limiting their practical application. We developed an optimized multiplex genome editing (MGE) toolbox that can efficiently assemble and stably express up to twelve
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Correction Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-24
Correction to ‘CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum’ Li, Y., Huang, C., Liu, Y., Zeng, J., Yu, H., Tong, Z., Yuan, X., et al. (2023) CRISPR/Cas9-mediated seamless gene replacement in protoplasts expands the resistance spectrum to TMV-U1 strain in regenerated Nicotiana tabacum. Plant Biotechnol
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Self‐incompatibility based functional genomics for rapid phenotypic characterization of seed metabolism genes Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-24 Abdul Azeez, Philip D. Bates
Reverse genetic characterization of plant gene function through technologies such as CRISPR/Cas, RNAi, or gene overexpression requires the ability to efficiently transform the plant species of interest. However, efficient transformation systems are not available for most plant species. Physaria fendleri [previously Lesquerella fendleri (Al-Shehbaz and O'Kane Jr., 2002)] is an emerging oilseed crop
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Pleiotropic phenotypic effects of the TaCYP78A family on multiple yield‐related traits in wheat Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-24 Meng Ma, Linnan Wu, Mengyao Li, Long Li, Lijian Guo, Deyan Ka, Tianxing Zhang, Mengdie Zhou, Baowei Wu, Haixia Peng, Zhaoxin Hu, Xiangli Liu, Ruilian Jing, Huixian Zhao
SummaryIncreasing crop yield depends on selecting and utilizing pleiotropic genes/alleles to improve multiple yield‐related traits (YRTs) during crop breeding. However, synergistic improvement of YRTs is challenging due to the trade‐offs between YRTs in breeding practices. Here, the favourable haplotypes of the TaCYP78A family are identified by analysing allelic variations in 1571 wheat accessions
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The extent of multiallelic, co‐editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-22 Eleanor J. Brant, Ayman Eid, Baskaran Kannan, Mehmet Cengiz Baloglu, Fredy Altpeter
SummarySugarcane (Saccharum spp. hybrid) is a prime feedstock for commercial production of biofuel and table sugar. Optimizing canopy architecture for improved light capture has great potential for elevating biomass yield. LIGULELESS1 (LG1) is involved in leaf ligule and auricle development in grasses. Here, we report CRISPR/Cas9‐mediated co‐mutagenesis of up to 40 copies/alleles of the putative LG1
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Genome editing of NPR3 confers potato resistance to Candidatus Liberibacter spp. Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-22 Manikandan Ramasamy, Mohan Singh Rajkumar, Renesh Bedre, Sonia Irigoyen, Katherine Berg‐Falloure, Michael V. Kolomiets, Kranthi K. Mandadi
Candidatus Liberibacter solanacearum (CLso) is a phloem-limited, fastidious bacterium associated with the potato (Solanum tuberosum) zebra chip disease. It is transmitted by the potato psyllid (Bactericera cockerelli Šulc.) and causes significant economic losses globally (Mora et al., 2021). Developing disease resistance by conventional breeding has shown limited success (Mora et al., 2022), thus necessitating
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KIN10‐mediated HB16 protein phosphorylation and self‐association improve cassava disease resistance by transcriptional activation of lignin biosynthesis genes Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-20 Yu Yan, Peng Wang, Jiaoyan He, Haitao Shi
SummaryCassava bacterial blight significantly affects cassava yield worldwide, while major cassava cultivars are susceptible to this disease. Therefore, it is crucial to identify cassava disease resistance gene networks and defence molecules for the genetic improvement of cassava cultivars. In this study, we found that MeHB16 transcription factor as a differentially expressed gene in cassava cultivars
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Light control of three‐dimensional chromatin organization in soybean Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-19 Zhu Li, Linhua Sun, Xiao Xu, Yutong Liu, Hang He, Xing Wang Deng
SummaryHigher‐order chromatin structure is critical for regulation of gene expression. In plants, light profoundly affects the morphogenesis of emerging seedlings as well as global gene expression to ensure optimal adaptation to environmental conditions. However, the changes and functional significance of chromatin organization in response to light during seedling development are not well documented
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An expanded cysteine‐rich receptor‐like kinase gene cluster functionally differentiates in drought, cold, heat, and pathogen stress responses in rice Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-19 Tiantian Ye, Huaijun Wang, Chengjing An, Haifu Tu, Lingqun Zhang, Dan Hu, Haiyan Xiong, Lizhong Xiong
Receptor-like kinases (RLKs) are crucial for plant stress responses. Cysteine-rich receptor-like kinases (CRKs), a significant RLK subfamily, typically possess a signal peptide, one to four extracellular DUF26 domains, a transmembrane domain, and an intracellular serine/threonine kinase domain (Chen, 2001). CRKs are land plant-specific, being present in vascular plants but absent in bryophytes and
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A genome‐wide association study uncovers a ZmRap2.7‐ZCN9/ZCN10 module to regulate ABA signalling and seed vigour in maize Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-18 Shasha Guo, Junmin Ai, Nannan Zheng, Hairui Hu, Zhuoyi Xu, Quanquan Chen, Li Li, Yunjun Liu, Hongwei Zhang, Jieping Li, Qingchun Pan, Fanjun Chen, Lixing Yuan, Junjie Fu, Riliang Gu, Jianhua Wang, Xuemei Du
SummarySeed vigour, including rapid, uniform germination and robust seedling establishment under various field conditions, is becoming an increasingly essential agronomic trait for achieving high yield in crops. However, little is known about this important seed quality trait. In this study, we performed a genome‐wide association study to identify a key transcription factor ZmRap2.7, which regulates
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Evolutionary relationship of moso bamboo forms and a multihormone regulatory cascade involving culm shape variation Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-14 Yan Liu, Chenglei Zhu, Xianghua Yue, Zeming Lin, Hui Li, Xiaolin Di, Jiangfei Wang, Zhimin Gao
SummaryMoso bamboo (Phyllostachys edulis) known as Mao Zhu (MZ) in Chinese exhibits various forms with distinct morphological characteristics. However, the evolutionary relationship among MZ forms and the mechanisms of culm shape variation are still lacking. Here, the main differences among MZ forms were identified as culm shape variation, which were confirmed by analysing MZ forms (799 bamboo culms)
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The complex relationship between disease resistance and yield in crops Plant Biotech. J. (IF 10.1) Pub Date : 2024-05-14 Mark C. Derbyshire, Toby E. Newman, William J. W. Thomas, Jacqueline Batley, David Edwards
SummaryIn plants, growth and defence are controlled by many molecular pathways that are antagonistic to one another. This results in a ‘growth‐defence trade‐off’, where plants temporarily reduce growth in response to pests or diseases. Due to this antagonism, genetic variants that improve resistance often reduce growth and vice versa. Therefore, in natural populations, the most disease resistant individuals