Aptamers are excellent recognition molecules obtained from systematic evolution of ligands by exponential enrichment (SELEX) that have been extensively researched for constructing aptasensors. However, in the process from SELEX to the construction of aptasensors, there are many disadvantages, such as tedious and repetitive operations, interference from external factors, and low efficiency, which seriously limits their application scope and development. Introducing the microfluidic technology can realize the integration and intelligence of SELEX and aptasensing, improve the efficiency of SELEX, and enhance the detection performance and convenience of aptasensing. Hence, in this review, the characteristics of various chips based on different driving forces are described firstly. And then summarizing the design of microfluidic devices based on different SELEX methods and showing the strategies of microfluidic aptasensors based on different detection modes. Finally, discussing the difficulties and challenges encountered when microfluidic is integrated with the SELEX and the aptasensors.

中文翻译：

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微流控技术在适配体 SELEX 和 Aptasensors 中的设计与应用


适配子是通过指数富集 （SELEX） 系统进化配体获得的优秀识别分子，已被广泛研究用于构建适配体传感器。然而，在从 SELEX 到 aptasensor 建设的过程中，存在许多缺点，例如繁琐和重复的操作、受外界因素干扰、效率低下，严重限制了它们的应用范围和发展。引入微流控技术可以实现 SELEX 与适配传感的集成化和智能化，提高 SELEX 的效率，增强适配传感的检测性能和便利性。因此，在本文中，首先描述了基于不同驱动力的各种芯片的特性。然后总结了基于不同 SELEX 方法的微流控器件设计，并展示了基于不同检测模式的微流控适配体传感器的策略。最后，讨论了微流体与 SELEX 和 aptasensors 集成时遇到的困难和挑战。