This review examines a wide range of marine microbial-derived bioactive peptide molecules, emphasizing the significance of reverse engineering in their production. The discussion encompasses the advancements in Marine Natural Products (MNPs) bio-manufacturing through the integration of omics-driven microbial engineering and bioinformatics. The distinctive features of non-ribosomally synthesised peptides (NRPs), and ribosomally synthesised precursor peptides (RiPP) biosynthesis is elucidated and presented. Additionally, the article delves into the origins of common peptide modifications. It highlights various genome mining approaches for the targeted identification of Biosynthetic Gene Clusters (BGCs) and novel RiPP and NRPs-derived peptides. The review aims to demonstrate the advancements, prospects, and obstacles in engineering both RiPP and NRP biosynthetic pathways.

中文翻译：

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用于工业应用的海洋生物活性肽分子的生产：逆向工程方法


这篇综述研究了多种海洋微生物衍生的生物活性肽分子，强调了逆向工程在其生产中的重要性。讨论内容包括通过组学驱动的微生物工程和生物信息学的整合在海洋天然产品（MNP）生物制造方面取得的进展。阐明并介绍了非核糖体合成肽 (NRP) 和核糖体合成前体肽 (RiPP) 生物合成的独特特征。此外，本文还深入探讨了常见肽修饰的起源。它重点介绍了用于生物合成基因簇 (BGC) 和新型 RiPP 和 NRP 衍生肽的靶向鉴定的各种基因组挖掘方法。该综述旨在展示 RiPP 和 NRP 生物合成途径工程的进展、前景和障碍。