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Temperature‐responsive hydrogel‐grafted vessel‐on‐a‐chip: Exploring cold‐induced endothelial injury Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-07-01 Chong Shen, Jiajie Li, Wenqi She, Aiping Liu, Qin Meng
Cold‐induced vasoconstriction is a significant contributor that leads to chilblains and hypothermia in humans. However, current animal models have limitations in replicating cold‐induced acral injury due to their low sensitivity to cold. Moreover, existing in vitro vascular chips composed of endothelial cells and perfusion systems lack temperature responsiveness, failing to simulate the vasoconstriction
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3D imaging and analysis to unveil the impact of microparticles on the pellet morphology of filamentous fungi Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-29 Anna Dinius, Henri Müller, Diana Kellhammer, Charlotte Deffur, Stefan Schmideder, Jörg U. Hammel, Rainer Krull, Heiko Briesen
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Reinforcement learning based temperature control of a fermentation bioreactor for ethanol production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-28 Nagabhushanamgari Rajasekhar, Thota Karunakaran Radhakrishnan, Samsudeen Naina Mohamed
Ethanol production is a significant industrial bioprocess for energy. The primary objective of this study is to control the process reactor temperature to get the desired product, that is, ethanol. Advanced model‐based control systems face challenges due to model‐process mismatch, but Reinforcement Learning (RL) is a class of machine learning which can help by allowing agents to learn policies directly
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Adaptive laboratory evolution in a novel parallel shaken pH‐auxostat Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-27 Burak Sarikaya, Hendrik Bück, Gino Pohen, Filipe Rodrigues, Karsten Günster, Katrin Wefelmeier, Katharina Miebach, Lars M. Blank, Jochen Büchs
Adaptive laboratory evolution (ALE) is a widely used microbial strain development and optimization method. ALE experiments, to select for faster‐growing strains, are commonly performed as serial batch cultivations in shake flasks, serum bottles, or microtiter plates or as continuous cultivations in bioreactors on a laboratory scale. To combine the advantages of higher throughput in parallel shaken
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Tailoring silk fibroin fibrous architecture by a high‐yield electrospinning method for fast wound healing possibilities Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-26 Jia‐Chen Zhu, Hui Wang, Chen‐Xing Wu, Ke‐Qin Zhang, Hua Ye
In this study, a novel array electrospinning collector was devised to generate two distinct regenerated silk fibroin (SF) fibrous membranes: ordered and disordered. Leveraging electrostatic forces during the electrospinning process allowed precise control over the orientation of SF fiber, resulting in the creation of membranes comprising both aligned and randomly arranged fiber layers. This innovative
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Chinese hamster ovary cell line engineering strategies for modular production of custom extracellular vesicles Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-25 Braulio Carrillo Sanchez, Matthew Hinchliffe, Mark Ellis, Catherine Simpson, David Humphreys, Bernie Sweeney, Daniel G. Bracewell
Continuously secreted by all cell types, extracellular vesicles (EVs) are small membrane‐bound structures which shuttle bioactive cargo between cells across their external environment. Their central role as natural molecular messengers and ability to cross biological barriers has garnered significant attention in the use of EVs as therapeutic delivery vehicles. Still, harnessing the potential of EVs
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Improvement of TaC9‐ABE mediated correction of human SMN2 gene Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-25 Xiaohua Peng, Yue Chi, Jinling Wang, Shuangpeng Li, Yang Liu, Chengcheng Tang, Xiaoqing Zhou, Xuan Lu, Yue Gao, Liangxue Lai, Min Chen, Qingjian Zou
Spinal muscular atrophy (SMA) is a devastating neuromuscular disease caused by mutations in the survival motor neuron 1 (SMN1) gene. Gene editing technology repairs the conversion of the 6th base T to C in exon 7 of the paralogous SMN2 gene, compensating for the SMN protein expression and promoting the survival and function of motor neurons. However, low editing efficiency and unintended off‐target
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Metabolomic characterization of monoclonal antibody‐producing Chinese hamster lung (CHL)‐YN cells in glucose‐controlled serum‐free fed‐batch operation Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Puriwat Sukwattananipaat, Hirotaka Kuroda, Noriko Yamano‐Adachi, Takeshi Omasa
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Development of a model for the ethanol concentration limit as a function of temperature and initial substrate concentration using the yeast Saccharomyces cerevisiae Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Ruan Dionízio SILVA, Flávio Luiz Honorato da Silva, Sharline Florentino de Melo Santos, Alberto Brandão Torres Neto, Julice Dutra Lopes, Josilene de Assis Cavalcante
In this study, a model was developed to simulate the effect of temperature () and initial substrate concentration () on the ethanol concentration limit () using the yeast Saccharomyces cerevisiae. To achieve this, regressions were performed using data provided by other authors for to establish a model dependent on and capable of predicting results with statistical significance. After constructing the
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Evaluation of bacteriophages for the alleviation of potential bacterial contamination risks in developmental engineering Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Yu Xiang, Xujin Bao, Tao Sun
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An industrial perspective on metabolic responses of Penicillium chrysogenum to periodic dissolved oxygen feast‐famine cycles in a scale‐down system Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Xueting Wang, Qi Yang, Cees Haringa, Zejian Wang, Ju Chu, Yingping Zhuang, Guan Wang
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Synergetic light and cytokinin treatments mitigate the recombinant protein yield depression induced by high‐density cultivation of hydroponically‐grown Nicotiana benthamiana Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-22 Stefano Bilotta, Gilbert Éthier, Ann‐Catherine Laliberté, Marie‐Claire Goulet, Michèle Martel, Dominique Michaud, Steeve Pepin
Plant molecular farming is currently operating a transition from soil‐based cultures toward hydroponic systems. In this study, we designed a whole‐plant NFT (nutrient film technique) platform for the transient expression of influenza virus‐like particles harboring hemagglutinin H1 proteins in Nicotiana benthamiana. In particular, we examined the effects of plant density during the post‐infiltration
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Improving the genetic stability of bacterial growth control for long-term bioproduction Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-15 Thibault Clavier, Corinne Pinel, Hidde de Jong, Johannes Geiselmann
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A multicrosslinked network composite hydrogel scaffold based on DLP photocuring printing for nasal cartilage repair Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-14 Wendan Jia, Zixian Liu, Lei Sun, Yanyan Cao, Zhizhong Shen, Meng Li, Yang An, Hulin Zhang, Shengbo Sang
Natural hydrogels are widely employed in tissue engineering and have excellent biodegradability and biocompatibility. Unfortunately, the utilization of such hydrogels in the field of three-dimensional (3D) printing nasal cartilage is constrained by their subpar mechanical characteristics. In this study, we provide a multicrosslinked network hybrid ink made of photocurable gelatin, hyaluronic acid,
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Commitment of human mesenchymal stromal cells towards ACL fibroblast differentiation upon rAAV-mediated FGF-2 and TGF-β overexpression using pNaSS-grafted PCL films Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-14 Meret Stehle, Mahnaz Amini, Jagadeesh K. Venkatesan, Wei Liu, Dan Wang, Tuan N. Nguyen, Amélie Leroux, Henning Madry, Véronique Migonney, Magali Cucchiarini
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Lentiviral vector determinants of anion-exchange chromatography elution heterogeneity Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-14 George Pamenter, Lee Davies, Ciaran Lamont, Danyal Rahim, Carol Knevelman, James Miskin, Kyriacos Mitrophanous, Duygu Dikicioglu, Daniel G. Bracewell
The demand for Lentiviral Vector (LV) drug substance is increasing. However, primary capture using convective anion-exchange chromatography remains a significant manufacturing challenge. This stems from a poor understanding of the complex adsorption behaviors linked to LVs intricate and variable structure, such as high binding heterogeneity which is typically characterized by a gradient elution profile
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Enhancing cell-based therapies with synthetic gene circuits responsive to molecular stimuli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-12 Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
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Pattern-centric transformation of omics data grounded on discriminative gene associations aids predictive tasks in TCGA while ensuring interpretability Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-10 André Patrício, Rafael S. Costa, Rui Henriques
The increasing prevalence of omics data sources is pushing the study of regulatory mechanisms underlying complex diseases such as cancer. However, the vast quantities of molecular features produced and the inherent interplay between them lead to a level of complexity that hampers both descriptive and predictive tasks, requiring custom-built algorithms that can extract relevant information from these
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Chromogenic fusion proteins as alternative textiles dyes Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-10 Tyson Watkins, Kaylee Moffitt, Robert E. Speight, Laura Navone
The widespread adoption of fast fashion has led to a significant waste problem associated with discarded textiles. Using proteins to color textiles can serve as a sustainable alternative to chemical dyes as well as reduce the demand for new raw materials. Here, we explore the use of chromogenic fusion proteins, consisting of a chromoprotein and a carbohydrate-binding module (CBM), as coloring agents
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Nanobiosensors based on on-site detection approaches for rapid pesticide sensing in the agricultural arena: A systematic review of the current status and perspectives Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-10 Subramani Srinivasan, Devarajan Raajasubramanian, Natarajan Ashokkumar, Veerasamy Vinothkumar, Nagarajan Paramaguru, Palanisamy Selvaraj, Ambothi Kanagalakshimi, Kuppan Narendra, Chinna Krishnan Shanmuga Sundaram, Raju Murali
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Effect of cellulose reducing ends and primary hydroxyl groups modifications on cellulose-cellulase interactions and cellulose hydrolysis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-10 Rajeev Kumar, Samarthya Bhagia, Ashutosh Mittal, Charles E. Wyman
Cellulose reducing ends are believed to play a vital role in the cellulose recalcitrance to enzymatic conversion. However, their role in insoluble cellulose accessibility and hydrolysis is not clear. Thus, in this study, reducing ends of insoluble cellulose derived from various sources were modified by applying reducing and/or oxidizing agents. The effects of cellulose reducing ends modification on
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Current state of implementation of in silico tools in the biopharmaceutical industry—Proceedings of the 5th modeling workshop Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-10 Felix Wittkopp, John Welsh, Robert Todd, Arne Staby, David Roush, Jessica Lyall, Sophie Karkov, Stephen Hunt, Jan Griesbach, Maria-Ona Bertran, Deenesh Babi
The fifth modeling workshop (5MW) was held in June 2023 at Favrholm, Denmark and sponsored by Recovery of Biological Products Conference Series. The goal of the workshop was to assemble modeling practitioners to review and discuss the current state, progress since the last fourth mini modeling workshop (4MMW), gaps and opportunities for development, deployment and maintenance of models in bioprocess
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Tailoring polishing steps for effective removal of polysorbate-degrading host cell proteins in antibody purification Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-10 Melanie Maier, Stefan Schneider, Linus Weiss, Simon Fischer, Daniel Lakatos, Joey Studts, Matthias Franzreb
Ensuring the quality and safety of biopharmaceutical products requires the effective separation of monoclonal antibodies (mAbs) from host cell proteins (HCPs). A major challenge in this field is the enzymatic hydrolysis of polysorbates (PS) in drug products. This study addresses this issue by investigating the removal of polysorbate-degrading HCPs during the polishing steps of downstream purification
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In vitro degradation, swelling, and bioactivity performances of in situ forming injectable chitosan-matrixed hydrogels for bone regeneration and drug delivery Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-05 Fatma Zehra Kocak, Muhammad Yar, Ihtesham U. Rehman
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Using ex vivo bioengineered lungs to model pathologies and screening therapeutics: A proof-of-concept study Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-04 Mohammadali Ahmadipour, Jorge Castilo Prado, Benyamin Hakak-Zargar, Malik Quasir Mahmood, Ian M. Rogers
Respiratory diseases, claim over eight million lives annually. However, the transition from preclinical to clinical phases in research studies is often hindered, partly due to inadequate representation of preclinical models in clinical trials. To address this, we conducted a proof-of-concept study using an ex vivo model to identify lung pathologies and to screen therapeutics in a humanized rodent model
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Elucidating solvent effects on lipase-catalyzed peroxyacid synthesis through activity-based kinetics and molecular dynamics Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-04 Salvador E. Brandolín, José A. Scilipoti, Ivana Magario
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Combining descriptive and predictive modeling to systematically design depth filtration-based harvest processes for biologics Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-04 Peter Liu, Michael Hartmann, Ajay Shankaran, Hong Li, John Welsh
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Multi-cell-line learning for the data-driven construction of mechanistic metabolic models Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-03 Yen-An Lu, Meghan G. McCann, Wei-Shou Hu, Qi Zhang
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Disposable electrochemical biosensors for the detection of bacteria in the light of antimicrobial resistance Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-01 Sreelakshmi C. S., Vrinda Kini, Maargavi Singh, Chiranjay Mukhopadhyay, Pooja Nag, Kapil Sadani
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Development of growth selection system and pocket engineering of d‐amino acid oxidase to enhance selective deamination activity toward d‐phosphinothricin Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-01 Feng Cheng, Ke‐Xiang Sun, Xiao‐Xiao Gong, Wei Peng, Hua‐Yue Zhang, Xi‐Hang Liang, Ya‐Ping Xue, Yu‐Guo Zheng
D‐amino acid oxidase (DAAO)‐catalyzed selective oxidative deamination is a very promising process for synthesizing l‐amino acids including l‐phosphinothricin ( l‐PPT, a high‐efficiency and broad‐spectrum herbicide). However, the wild‐type DAAO's low activity toward unnatural substrates like d‐phosphinothricin ( d‐PPT) hampers its application. Herein, a DAAO from Caenorhabditis elegans (CeDAAO) was
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Dynamic optimization of an integrated cultivation‐aggregation model for mAb production Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-06-01 Wil Jones, Dimitrios I. Gerogiorgis
Due to their proteinaceous structure, monoclonal antibodies (mAbs) are susceptible to irreversible aggregation, with harmful consequences on drug efficacy and patient safety. To mitigate this risk in modern biopharmaceutical processes, it is critical to comply with current good manufacturing practices (cGMP) and pursue operating strategies minimizing irreversible aggregation whilst also maximizing
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Reinforcement learning‐guided control strategies for CAR T‐cell activation and expansion Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-30 Sakib Ferdous, Ibne Farabi Shihab, Ratul Chowdhury, Nigel F. Reuel
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Removal of empty capsids from high‐dose adeno‐associated virus 9 gene therapies Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-29 William S. Kish, John Lightholder, Tamara Zeković, Alex Berrill, Matthew Roach, William B. Wellborn, Eric Vorst
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Cover Image, Volume 121, Number 6, June 2024 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-28 Chuan‐Chieh Hsiang, I‐Son Ng
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Biotechnology and Bioengineering: Volume 121, Number 6, June 2024 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-28
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Status and future developments for downstream processing of biological products Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-25 Alois Jungbauer, Gisela Ferreira, Michelle Butler, Susan D'Costa, Kevin Brower, Andrea Rayat, Richard Willson
Governments and biopharmaceutical organizations aggressively leveraged expeditious communication capabilities, decision models, and global strategies to make a COVID‐19 vaccine happen within a period of 12 months. This was an unusual effort and cannot be transferred to normal times. However, this focus on a single vaccine has also led to other treatments and drug developments being sidelined. Society
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CRISPR‐Cas9 mediated genome editing of Kluyveromyces marxianus for iterative, multiplexed gene disruption and pathway integration Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-23 Wenliang Wang, Xinkai Wang, Yadi Tan, Shuo Zhao, Liqian Zhao, Zhiwei Zhu
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Expanding the synthetic biology repertoire of a fast‐growing cyanobacterium Synechococcus elongatus PCC 11801 Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-22 Swati Madhu, Annesha Sengupta, Aditya P. Sarnaik, Pramod P. Wangikar
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A new methodology for a rapid and high‐throughput comparison of molecular profiles and biological activity of phytoextracts Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-18 Viviana Caldeira, Tiago A. H. Fonseca, Luana N'Dembo, Rúben Araújo, Cristiana P. Von Rekowski, Pedro N. S. Sampaio, Cecília R.C. Calado
To robustly discover and explore phytocompounds, it is necessary to evaluate the interrelationships between the plant species, plant tissue, and the extraction process on the extract composition and to predict its cytotoxicity. The present work evaluated how Fourier Transform InfraRed spectroscopy can acquire the molecular profile of aqueous and ethanol‐based extracts obtained from leaves, seeds, and
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A perfusion host‐microbe bioreactor (HMB) system that captures dynamic interactions of secreted metabolites between epithelial cells cocultured with a human gut anaerobe Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-08 Jingyun Yang, Jason Cassaday, Thomas P. Wyche, Brian Squadroni, William Newhard, Huong Trinh, Damien Cabral, Erik Hett, Theodore R. Sana, Kyongbum Lee, Stephen Kasper
The human microbiota impacts a variety of diseases and responses to therapeutics. Due to a lack of robust in vitro models, detailed mechanistic explanations of host‐microbiota interactions cannot often be recapitulated. We describe the design and development of a novel, versatile and modular in vitro system that enables indirect coculture of human epithelial cells with anaerobic bacteria for the characterization
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Vaccine process technology—A decade of progress Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-07 Barry Buckland, Gautam Sanyal, Todd Ranheim, David Pollard, Jim A. Searles, Sue Behrens, Stefanie Pluschkell, Jessica Josefsberg, Christopher J. Roberts
In the past decade, new approaches to the discovery and development of vaccines have transformed the field. Advances during the COVID‐19 pandemic allowed the production of billions of vaccine doses per year using novel platforms such as messenger RNA and viral vectors. Improvements in the analytical toolbox, equipment, and bioprocess technology have made it possible to achieve both unprecedented speed
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Higher efficiency of vanadate iron in heterogeneous Fenton‐like systems to pretreat sugarcane bagasse and its enzymatic saccharification Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-07 Ju Liang, Huiying Zeng, Yuting Zhang, Wenbing Zhou, Naidong Xiao
Pretreatment is crucial for effective enzymatic saccharification of lignocellulose such as sugarcane bagasse (SCB). In the present study, SCB was pretreated with five kinds of heterogeneous Fenton‐like systems (HFSs), respectively, in which α‐FeOOH, α‐Fe2O3, Fe3O4, and FeS2 worked as four traditional heterogeneous Fenton‐like catalysts (HFCs), while FeVO4 worked as a novel HFC. The enzymatic reducing
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Computational fluid dynamics based digital twins of fixed bed bioreactors validate scaling principles for recombinant adeno‐associated virus gene therapy manufacturing Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-06 Michael Hill, Colten White, Shaoying Wang, John Thomas, Brian DeVincentis, Nripen Singh
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Evaluation of various membranes at different fluxes to enable large‐volume single‐use perfusion bioreactors Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-04 Hassan Raza, Tiffany Tang, Baizhen Gao, Chelsea Phuangthong, Chienhung Billy Chen, Nuno D. S. Pinto
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Reinventing shake flask fermentation: The breathable flask Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-03 Vikash Kumar, Michael Tolosa, Xudong Ge, Govind Rao
Cultivating cells in shake flasks is a routine operation that is largely unchanged since its inception. A glass or plastic Erlenmeyer vessel with the primary gas exchange taking place across various porous plugs is used with media volumes typically ranging from 100 mL to 2 L. Oxygen limitation and carbon dioxide accumulation in the vessel is a major concern for studies involving shake flask cultures
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Mechanistic modeling of in vitro transcription incorporating effects of magnesium pyrophosphate crystallization Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-05-02 Nathan Merica Stover, Krystian Ganko, Richard D. Braatz
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Rapid spectrophotometric detection for optimized production of landomycins and characterization of their therapeutic potential Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-30 Todd C. Chappell, Kathleen G. Maiello, Allison J. Tierney, Karin Yanagi, Jessica A. Lee, Kyongbum Lee, Charles R. Mace, Clay S. Bennett, Nikhil U. Nair
Microbial‐derived natural products remain a major source of structurally diverse bioactive compounds and chemical scaffolds that have the potential as new therapeutics to target drug‐resistant pathogens and cancers. In particular, genome mining has revealed the vast number of cryptic or low‐yield biosynthetic gene clusters in the genus Streptomyces. However, low natural product yields—improvements
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Hotspot Wizard-informed engineering of a hyperthermophilic β-glucosidase for enhanced enzyme activity at low temperatures Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-29 Mehmet Emre Erkanli, Khalid El-Halabi, Ted Keunsil Kang, Jin Ryoun Kim
Hyperthermophilic enzymes serve as an important source of industrial enzymes due to their high thermostability. Unfortunately, most hyperthermophilic enzymes suffer from reduced activity at low temperatures (e.g., ambient temperature), limiting their applicability. In addition, evolving hyperthermophilic enzymes to increase low temperature activity without compromising other desired properties is generally
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Listening to life: Sonification for enhancing discovery in biological research Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-28 Rhea Braun, Maxwell Tfirn, Roseanne M. Ford
Sonification, or the practice of generating sound from data, is a promising alternative or complement to data visualization for exploring research questions in the life sciences. Expressing or communicating data in the form of sound rather than graphs, tables, or renderings can provide a secondary information source for multitasking or remote monitoring purposes or make data accessible when visualizations
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Bioprocess in-line monitoring and control using Raman spectroscopy and Indirect Hard Modeling (IHM) Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-28 David Heinrich Müller, Marieke Börger, Julia Thien, Hans-Jürgen Koß
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Structural and molecular insights into a bifunctional glycoside hydrolase 30 xylanase specific to glucuronoxylan Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-28 Christina Pentari, Christos Kosinas, Efstratios Nikolaivits, Maria Dimarogona, Evangelos Topakas
Glycoside hydrolase (GH) 30 family xylanases are enzymes of biotechnological interest due to their capacity to degrade recalcitrant hemicelluloses, such as glucuronoxylan (GX). This study focuses on a subfamily 7 GH30, TtXyn30A from Thermothelomyces thermophilus, which acts on GX in an “endo” and “exo” mode, releasing methyl-glucuronic acid branched xylooligosaccharides (XOs) and xylobiose, respectively
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Correction to “Metabolic engineering of betacyanin in vegetables for anti-inflammatory therapy” Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-28
Saito, S., Nishihara, M., Kohakura, M., Kimura, K., Yashiro, T., Takasawa, S., & Arimura, G.-i. (2023). Metabolic engineering of betacyanin in vegetables for anti-inflammatory therapy. Biotechnology and Bioengineering, 120, 1357–1365. https://doi.org/10.1002/bit.28335 In the originally published article, in Section 2.8, second paragraph, “4,4-dimethyl-4-silapentane-1-sulfonic acid” should read “dextran
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Systems engineering Escherichia coli for efficient production p-coumaric acid from glucose Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-26 Chong Qiu, Xiaoge Wang, Jiaojiao Zuo, Runyang Li, Cong Gao, Xiulai Chen, Jia Liu, Wanqing Wei, Jing Wu, Guipeng Hu, Wei Song, Nan Xu, Liming Liu
P-coumaric acid (p-CA), a pant metabolite with antioxidant and anti-inflammatory activity, is extensively utilized in biomedicine, food, and cosmetics industry. In this study, a synthetic pathway (PAL) for p-CA was designed, integrating three enzymes (AtPAL2, AtC4H, AtATR2) into a higher l-phenylalanine-producing strain Escherichia coli PHE05. However, the lower soluble expression and activity of AtC4H
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Synthesized arsenic nanoparticles and their high potential in biomedical applications: A review Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-25 Seyed Soheil Hosseininasab, Mahin Naderifar, Majid Reza Akbarizadeh, Nooshin Hashemi, Mehdi Ghaderi, Hamid Pajavand, Naghmeh Satarzadeh, Amin Sadeghi Dousari
Arsenic with the scientific name AS is an element that exists everywhere. It is the fourth among the abundant elements in water, the twelfth in the human body, and the twentieth in the earth's crust. This element exists in sulfide, carbonate, and elemental forms. Different names of arsenic are known as white arsenic (As2O3), yellow arsenic (As2S3), and red arsenic (As4S4). Nowadays, due to its unique
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Covalent organic frameworks (COFs) as carrier for improved drug delivery and biosensing applications Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-25 Rida Younas, Farhat Jubeen, Nargis Bano, Silvana Andreescu, Hongxia Zhang, Akhtar Hayat
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Super7 passaging method to improve Chinese hamster ovary cell fed‐batch performance Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-25 Matthew J. Moran, Jin Chen, James M. Piret, R. Robert Balcarcel
Chinese hamster ovary (CHO) cells are widely used to manufacture biopharmaceuticals, most of all monoclonal antibodies (mAbs). Some CHO cell lines exhibit production instability, where the productivity of the cells decreases as a function of time in culture. To counter this, we designed a passaging strategy that, rather than maximizing the time spent in log‐growth phase, mimics the first 7 days of
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Integration of just-in-time learning with variational autoencoder for cell culture process monitoring based on Raman spectroscopy Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-23 Mohammad Rashedi, Hamid Khodabandehlou, Tony Wang, Matthew Demers, Aditya Tulsyan, Christopher Garvin, Cenk Undey
Protein production in the biopharmaceutical industry necessitates the utilization of multiple analytical techniques and control methodologies to ensure both safety and consistency. To facilitate real-time monitoring and control of cell culture processes, Raman spectroscopy has emerged as a versatile analytical technology. This technique, categorized as a Process Analytical Technology, employs chemometric
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Stabilization of galactose oxidase by high hydrostatic pressure: Insights on the role of cavities size Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-22 Min J. Kang, José I. Reyes-De-Corcuera
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Development of generic metabolic Raman calibration models using solution titration in aqueous phase and data augmentation for in-line cell culture analysis Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-19 Zhijun Zhang, Zhe Lang, Gong Chen, Hang Zhou, Weichang Zhou
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Engineering of the Lrp/AsnC-type transcriptional regulator DecR as a genetically encoded biosensor for multilevel optimization of L-cysteine biosynthesis pathway in Escherichia coli Biotechnol. Bioeng. (IF 3.5) Pub Date : 2024-04-18 Zhiyou Zhou, Zonglin Li, Yahui Zhong, Shuai Xu, Zhimin Li