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From Sensing to Acclimation: The Role of Membrane Lipid Remodeling in Plant Responses to Low Temperatures Plant Physiol. (IF 6.5) Pub Date : 2024-07-19 Zachery D Shomo, Fangyi Li, Cailin N Smith, Sydney R Edmonds, Rebecca L Roston
Low temperatures pose a dramatic challenge to plant viability. Chilling and freezing disrupt cellular processes, forcing metabolic adaptations reflected in alterations to membrane compositions. Understanding the mechanisms of plant cold tolerance is increasingly important due to anticipated increases in the frequency, severity, and duration of cold events. This review synthesizes current knowledge
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Nuclear factors NF-YC3 and NF-YBs positively regulate arbuscular mycorrhizal symbiosis in tomato Plant Physiol. (IF 6.5) Pub Date : 2024-07-19 Heng Chien, Ting-Yu Kuo, Ching-Hung Yao, Yi-Ru Su, Yu-Ting Chang, Zheng-Lin Guo, Kai-Chieh Chang, Yu-Heng Hsieh, Shu-Yi Yang
The involvement of nuclear factor Y (NF-Y) in transcriptional reprogramming during arbuscular mycorrhizal symbiosis has been demonstrated in several plant species. However, a comprehensive picture is lacking. We showed that the spatial expression of NF-YC3 was observed in cortical cells containing arbuscules via the cis-regulatory element GCC boxes. Moreover, the NF-YC3 promoter was transactivated
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RNA N6-adenine methylation dynamics impact Hyaloperonospora arabidopsidis resistance in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2024-07-12 Leonardo Furci, Jérémy Berthelier, Hidetoshi Saze
In plants, epitranscriptomic mark N6-adenine methylation (m6A) is dynamically regulated in response to environmental cues. However, little is known about m6A dynamics under biotic stresses and their role in environmental adaptation. Additionally, current methodologies limit the investigation of m6A dynamics at single-nucleotide resolution on specific RNA molecules. Using Oxford Nanopore Technology
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Bi-parental graph strategy to represent and analyze hybrid plant genomes Plant Physiol. (IF 6.5) Pub Date : 2024-07-12 Qianqian Kong, Yi Jiang, Mingfei Sun, Yunpeng Wang, Lin Zhang, Xing Zeng, Zhiheng Wang, Zijie Wang, Yuting Liu, Yuanxian Gan, Han Liu, Xiang Gao, Xuerong Yang, Xinyuan Song, Hongjun Liu, Junpeng Shi
Hybrid plants are found extensively in the wild, and they often demonstrate superior performance of complex traits over their parents and other selfing plants. This phenomenon, known as heterosis, has been extensively applied in plant breeding for decades. However, the process of decoding hybrid plant genomes has seriously lagged due to the challenges associated with genome assembly and the lack of
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Myo-inositol oxygenase CgMIOX3 alleviates S-RNase-induced inhibition of incompatible pollen tubes in pummelo Plant Physiol. (IF 6.5) Pub Date : 2024-07-12 Qiang Xu, Chenchen Liu, Zhezhong Zhang, Zonghong Cao, Mei Liang, Changning Ye, Zongcheng Lin, Xiuxin Deng, Junli Ye, Maurice Bosch, Lijun Chai
Pummelo (Citrus grandis L. Osbeck) exhibits S-RNase-based self-incompatibility (SI), during which S-RNase cytotoxicity inhibits pollen tubes in an S-haplotype specific manner. The entry of S-RNase into self-pollen tubes triggers a series of reactions. However, these reactions are still poorly understood in pummelo. In the present study, we used S-RNases as baits to screen a pummelo pollen cDNA library
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DWARF AND LESS TILLERS ON CHROMOSOME 3 Promotes Tillering in Rice by Sustaining FLORAL ORGAN NUMBER 1 Expression Plant Physiol. (IF 6.5) Pub Date : 2024-07-12 Yongyi Fan, Hongmei Chen, Beifang Wang, Dian Li, Ran Zhou, Wangmin Lian, Gaoneng Shao, Xiangjin Wei, Weixun Wu, Qunen Liu, Lianping Sun, Xiaodeng Zhan, Shihua Cheng, Yingxin Zhang, Liyong Cao
Three key factors determine yield in rice (Oryza sativa): panicle number, grain number, and grain weight. Panicle number is strongly associated with tiller number. Although many genes regulating tillering have been identified, whether Dof proteins are involved in controlling plant architecture remains unknown. The dwarf and less tillers on chromosome 3 (dlt3) rice mutant produces fewer tillers than
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CYTOSOLIC INVERTASE2 regulates flowering and reactive oxygen species-triggered programmed cell death in tomato Plant Physiol. (IF 6.5) Pub Date : 2024-07-12 Qiongqiong Zhang, Xi Wang, Tianying Zhao, Junfeng Luo, Xin Liu, Jing Jiang
Cytosolic invertase (CIN) in plants hydrolyzes sucrose into fructose and glucose, influencing flowering time and organ development. However, the underlying molecular mechanisms remain elusive. Through expressional, genetic, and histological analyses, we identified a substantially role of SlCIN2 (localized in mitochondria) in regulating flowering and pollen development in tomato (Solanum lycopersicum)
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Effector MoSDT1 enhances Magnaporthe oryzae virulence and plays a dual role in regulating rice defense Plant Physiol. (IF 6.5) Pub Date : 2024-07-09 Hongfeng Chen, Shunyu Su, Shumin Yang, Tianqi Zhao, Ping Tang, Qiong Luo, Yuanyuan Zhong, Jing Yang
C2H2 zinc effectors are a class of pathogen proteins that play a dual role in plant-pathogen interactions, promoting pathogenicity and enhancing plant defense. In our previous research, we identified MoSDT1 (Magnaporthe Oryzae Systemic Defense Trigger 1) as a C2H2 zinc effector that activates rice (Oryza sativa) defense when overexpressed in rice. However, its regulatory roles in pathogenicity and
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Two Calcium Sensor-Activated Kinases Function in Root Hair Growth Plant Physiol. (IF 6.5) Pub Date : 2024-07-09 Xianming Fang, Beibei Liu, Haiyan Kong, Jingyou Zeng, Yixin Feng, Chengbin Xiao, Qianshuo Shao, Xuemei Huang, Yujun Wu, Aike Bao, Jia Li, Sheng Luan, Kai He
Plant pollen tubes and root hairs typical polarized tip growth. It is well established that calcium ions (Ca2+) play essential roles in maintaining cell polarity and guiding cell growth orientation. Ca2+ signals are encoded by Ca2+ channels and transporters and are decoded by a variety of Ca2+-binding proteins often called Ca2+ sensors, in which calcineurin B-like protein (CBL) proteins function by
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PICKLE and HISTONE DEACETYLASE6 coordinately regulate genes and transposable elements in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2024-07-08 Wenjuan Li, Xiaoling Zhang, Qingche Zhang, Qingzhu Li, Yanzhuo Li, Yanfang Lv, Yue Liu, Ying Cao, Huamei Wang, Xiangsong Chen, Hongchun Yang
Chromatin dynamics play essential roles in transcriptional regulation. The chromodomain helicase DNA-binding domain 3 (CHD3) chromatin remodeler PICKLE (PKL) and HISTONE DEACETYLASE6 (HDA6) are required for transcriptional gene silencing, but their coordinated function in gene repression requires further study. Through a genetic suppressor screen, we found that a point mutation at PKL could partially
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Light-responsive transcription factors VvHYH and VvGATA24 mediate wax terpenoid biosynthesis in Vitis vinifera Plant Physiol. (IF 6.5) Pub Date : 2024-07-08 Mingyi Yang, Yizhou Xiang, Zisheng Luo, Yizhou Gao, Lei Wang, Qiannan Hu, Yingying Dong, Ming Qi, Dong Li, Lingling Liu, Miroslava Kačániová, Zhaojun Ban, Li Li
The cuticular wax that covers the surfaces of plants is the first barrier against environmental stresses and increasingly accumulates with light exposure. However, the molecular basis of light-responsive wax biosynthesis remains elusive. In grape (Vitis vinifera), light exposure resulted in higher wax terpenoid content and lower decay and abscission rates than controls kept in darkness. Assay for
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GLUTAMYL-tRNA SYNTHETASE 1 deficiency confers thermo-sensitive male sterility in rice by affecting ROS homeostasis Plant Physiol. (IF 6.5) Pub Date : 2024-07-08 Huixin Liu, Hanli You, Changzhen Liu, Yangzi Zhao, Jiawei Chen, Zhuoran Chen, Yafei Li, Ding Tang, Yi Shen, Zhukuan Cheng
Temperature is one of the key environmental factors influencing crop fertility and yield. Understanding how plants sense and respond to temperature changes is, therefore, crucial for improving agricultural production. In this study, we characterized a temperature-sensitive male-sterile mutant in rice (Oryza sativa), glutamyl-tRNA synthetase 1-2 (ers1-2), that shows reduced fertility at high temperatures
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Cell wall integrity modulates HOOKLESS1 and PHYTOCHROME INTERACTING FACTOR4 expression controlling apical hook formation Plant Physiol. (IF 6.5) Pub Date : 2024-07-08 Riccardo Lorrai, Özer Erguvan, Sara Raggi, Kristoffer Jonsson, Jitka Široká, Danuše Tarkowská, Ondřej Novák, Jayne Griffiths, Alexander M Jones, Stéphane Verger, Stéphanie Robert, Simone Ferrari
Formation of the apical hook in etiolated dicot seedlings results from differential growth in the hypocotyl apex and is tightly controlled by environmental cues and hormones, among which auxin and gibberellins (GAs) play an important role. Cell expansion is tightly regulated by the cell wall, but whether and how feedback from this structure contributes to hook development is still unclear. Here, we
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A disease resistance assay in Nicotiana benthamiana reveals the immune function of Response to HopBA1 Plant Physiol. (IF 6.5) Pub Date : 2024-07-08 Keiichi Hasegawa, Ton Timmers, Jijie Chai, Takaki Maekawa
A receptor protein variant lacking 2',3'-cAMP/cGMP synthetase activity but retaining NADase activity does not induce cell death but confers resistance to Potato Virus X.
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Soybean ethylene response factors GmENS1 and GmENS2 promote nodule senescence Plant Physiol. (IF 6.5) Pub Date : 2024-07-02 Aifang Xiao, Jiashan Wu, Weiyun Wang, Yuxin Guan, Mengting Zhuang, Xiaoli Guo, Hui Zhu, Haixiang Yu, Yangrong Cao
The final phase in root nodule development is nodule senescence. The mechanism underlying the initiation of nodule senescence requires further elucidation. Here, we investigated the intrinsic signals governing soybean (Glycine max L. Merr.) nodule senescence, uncovering ethylene as a key signal in this intricate mechanism. Two AP2/ERF transcription factor genes, GmENS1 and GmENS2 (Ethylene-responsive
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FATTY ACID DESATURASE4 enhances plant RNA virus replication and undergoes host vacuolar ATPase-mediated degradation Plant Physiol. (IF 6.5) Pub Date : 2024-06-27 Xinxin Fang, Zhaoxing Jia, Tianqi Yu, Penghuan Rui, Hongying Zheng, Yuwen Lu, Jiejun Peng, Shaofei Rao, Jian Wu, Jianping Chen, Fei Yan, Guanwei Wu
Emerging evidence indicates that fatty acid (FA) metabolic pathways regulate host immunity to vertebrate viruses. However, information on FA signaling in plant virus infection remains elusive. In this study, we demonstrate the importance of fatty acid desaturase (FAD), an enzyme that catalyzes the rate-limiting step in the conversion of saturated FAs into unsaturated FAs, during infection by a plant
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The Arabidopsis ARID-HMG DNA-BINDING PROTEIN 15 modulates JA signaling by regulating MYC2 during pollen development Plant Physiol. (IF 6.5) Pub Date : 2024-06-26 Sonal Sachdev, Ruby Biswas, Adrita Roy, Ayantika Nandi, Vishal Roy, Sabini Basu, Shubho Chaudhuri
The intricate process of male gametophyte development in flowering plants is regulated by jasmonic acid (JA) signaling. JA signaling initiates with the activation of the basic-helix-loop-helix (bHLH) transcription factor (TF), MYC2, leading to the expression of numerous JA-responsive genes during stamen development and pollen maturation. However, the regulation of JA signaling during different stages
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Photoreceptor-induced sinapate synthesis contributes to photoprotection in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2024-06-26 Manuela Leonardelli, Nicolas Tissot, Roman Podolec, Florence Ares-Orpel, Gaétan Glauser, Roman Ulm, Emilie Demarsy
Plants must balance light capture for photosynthesis with protection from potentially harmful ultraviolet radiation (UV). Photoprotection is mediated by concerted action of photoreceptors, but the underlying molecular mechanisms are not fully understood. In this study, we provide evidence that UV RESISTANCE LOCUS 8 (UVR8) UV-B-, phytochrome red-, and cryptochrome blue-light photoreceptors converge
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Filling the gaps on root hair development under salt stress and phosphate starvation using current evidence coupled with a meta-analysis approach Plant Physiol. (IF 6.5) Pub Date : 2024-06-26 Miguel Angel Ibeas, Hernán Salinas-Grenet, Nathan R Johnson, Jorge Pérez-Díaz, Elena A Vidal, José Miguel Alvarez, José M Estevez
Population expansion is a global issue, especially for food production. Meanwhile, global climate change is damaging our soils, making it difficult for crops to thrive and lowering both production and quality. Poor nutrition and salinity stress affect plant growth and development. Although the impact of individual plant stresses has been studied for decades, the real stress scenario is more complex
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Cucumber JASMONATE ZIM-DOMAIN 6 interaction with transcription factor MYB6 impairs waterlogging-triggered adventitious rooting Plant Physiol. (IF 6.5) Pub Date : 2024-06-26 Jiawei Pan, Hamza Sohail, Rahat Sharif, Qiming Hu, Jia Song, Xiaohua Qi, Xuehao Chen, Xuewen Xu
Waterlogging is a serious abiotic stress that drastically decreases crop productivity by damaging the root system. Jasmonic acid (JA) inhibits waterlogging-induced adventitious root (AR) formation in cucumber (Cucumis sativus L.). However, we still lack a profound mechanistic understanding of how JA governs AR formation under waterlogging stress. JAZ (JASMONATE ZIM-DOMAIN) proteins are responsible
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Pistil-derived lipids influence pollen tube growth and male fertility in Arabidopsis thaliana Plant Physiol. (IF 6.5) Pub Date : 2024-06-25 Jingpu Song, Ioannis Mavraganis, Wenyun Shen, Hui Yang, Nii Patterson, Liping Wang, Daoquan Xiang, Yuhai Cui, Jitao Zou
Pollen germination and pollen tube elongation require rapid phospholipid production and remodeling in membrane systems that involve both de novo synthesis and turnover. Phosphatidic acid phosphohydrolase (PAH) and lysophosphatidylcholine acyltransferase (LPCAT) are two key enzymes in membrane lipid maintenance. PAH generates diacylglycerol (DAG), a necessary precursor for the de novo synthesis of phosphatidylcholine
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A potyvirus provides an efficient viral vector for gene expression and functional studies in Asteraceae plants Plant Physiol. (IF 6.5) Pub Date : 2024-06-25 Yi-Zhou Yang, Liang Xie, Qiang Gao, Zhang-Yao Nie, Ding-Liang Zhang, Xian-Bing Wang, Cheng-Gui Han, Ying Wang
Plant virus-derived vectors are rapid and cost-effective for protein expression and gene functional studies in plants, particularly for species that are difficult to genetically transform. However, few efficient viral vectors are available for functional studies in Asteraceae plants. Here, we identified a potyvirus named zinnia mild mottle virus (ZiMMV) from common zinnia (Zinnia elegans Jacq.) through
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Genome-wide characterization of single-stranded DNA in rice Plant Physiol. (IF 6.5) Pub Date : 2024-06-25 Yulian Peng, Pengtao Zhao, Zhaoguo Li, Ning Mu, Shentong Tao, Yilong Feng, Xuejiao Cheng, Wenli Zhang
Single-stranded DNA (ssDNA) is essential for various DNA-templated processes in both eukaryotes and prokaryotes. However, comprehensive characterizations of ssDNA still lag in plants compared to non-plant systems. Here, we conducted in situ S1-seq (ISS1-seq), with starting gDNA ranging from 5 µg to 250 ng, followed by comprehensive characterizations of ssDNA in rice (Oryza sativa L.). We found that
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Genetic factors of grain cadmium concentration in Polish wheat (Triticum polonicum L.) Plant Physiol. (IF 6.5) Pub Date : 2024-06-25 Yiran Cheng, Rui Liu, Tian Yang, Shan Yang, Jia Chen, Yiwen Huang, Dan Long, Jian Zeng, Dandan Wu, Houyang Kang, Xing Fan, Lina Sha, Haiqin Zhang, Yonghong Zhou, Yi Wang
Wheat (Triticum aestivum L.) is one of the most important crops worldwide and a major source of human Cd intake. Limiting grain Cd concentration (Gr_Cd_Conc) in wheat is necessary to ensure food safety. However, the genetic factors associated with Cd uptake, translocation, distribution, and Gr_Cd_Conc in wheat are poorly understood. Here, we mapped quantitative trait loci (QTL) for Gr_Cd_Conc and its
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DWARF TILLER1 regulates apical–basal pattern formation and proper orientation of rice embryos Plant Physiol. (IF 6.5) Pub Date : 2024-06-21 Jingyao Tang, Xiaorong Huang, Mengxiang Sun, Wanqi Liang
Body axis establishment is one of the earliest patterning events in plant embryogenesis. Asymmetric zygote division is critical for apical–basal axis formation in Arabidopsis (Arabidopsis thaliana). However, how the orientation of the cell division plane is regulated and its relation to apical–basal axis establishment and proper position of embryos in grasses remain poorly understood. By characterizing
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The CsDof1.8-CsLIPOXYGENASE09 module regulates C9 aroma production in cucumber Plant Physiol. (IF 6.5) Pub Date : 2024-06-14 Yinhui Sun, Xuzhen Li, Hua Wang, Qiongzhi Zhang, Xin Wang, Yanan Jiao, Jie Zhang, Yuying Yang, Wanyu Xue, Yulei Qian, Xiaojiang Zhang, Ruochen Wang, Shuxia Chen
Nine-carbon aldehydes and their relative alcohols (C9 aromas) are the main aroma compounds of cucumber (Cucumis sativus L.) fruits and provide a unique cucumber-like note. However, the key regulators of C9 aroma accumulation in cucumber fruit are poorly characterized. Based on C9 aroma dynamic analysis and transcriptome analysis during fruit development of two different cucumber inbred lines, Q16 and
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SUPPRESSOR OF FRIGIDA 4 cooperates with the histone methylation reader EBS to positively regulate root development Plant Physiol. (IF 6.5) Pub Date : 2024-06-14 Can Huang, Diao Wang, Yanqi Yang, Hong Yang, Biaoming Zhang, Haitao Li, Haitao Zhang, Yan Li, Wenya Yuan
Maintenance and homeostasis of the quiescent center (QC) in Arabidopsis (Arabidopsis thaliana) root apical meristems are critical for stem cell organization and root development. Despite great progress in relevant research, the molecular mechanisms that determine the root stem cell fate and QC still need further exploration. In Arabidopsis, SUPPRESSOR OF FRIGIDA 4 (SUF4) encodes a C2H2-type zinc finger
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LUX ARRHYTHMO links CBF pathway and jasmonic acid metabolism to regulate cold tolerance of tea plants Plant Physiol. (IF 6.5) Pub Date : 2024-06-14 Yanli Wang, Wei Tong, Fangdong Li, Lidia Samarina, Penghui Li, Tianyuan Yang, Zhaoliang Zhang, Lianghui Yi, Fei Zhai, Xinchao Wang, Enhua Xia
Cold stress declines the quality and yield of tea, yet the molecular basis underlying cold tolerance of tea plants (Camellia sinensis) remains largely unknown. Here, we identified a circadian rhythm component LUX ARRHYTHMO (LUX) that potentially regulates cold tolerance of tea plants through a genome-wide association study and transcriptomic analysis. The expression of CsLUX phased with sunrise and
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Transcriptome and metabolome atlas reveals contributions of sphingosine and chlorogenic acid to cold tolerance in Citrus Plant Physiol. (IF 6.5) Pub Date : 2024-06-14 Peng Xiao, Jing Qu, Yue Wang, Tian Fang, Wei Xiao, Yilei Wang, Yu Zhang, Madiha Khan, Qiyu Chen, Xiaoyong Xu, Chunlong Li, Ji-Hong Liu
Citrus is one of the most important fruit crop genera in the world, but many Citrus species are vulnerable to cold stress. Ichang papeda (Citrus ichangensis), a cold-hardy citrus species, holds great potential for identifying valuable metabolites that are critical for cold tolerance in Citrus. However, the metabolic changes and underlying mechanisms that regulate Ichang papeda cold tolerance remain
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Running Fermi calculations as a superpower to gauge reality Plant Physiol. (IF 6.5) Pub Date : 2024-06-14 Edmar R Oliveira-Filho, Rodrigo Campos-Silva, Andrew D Hanson
Making Fermi calculations is a way to estimate rough but reliable numbers that enable right reasoning in science and engineering – and a skill that all scientists should practice.
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Evaluation of maize varieties via multivariate analysis: Roles of ionome, antioxidants, and autophagy in salt tolerance Plant Physiol. (IF 6.5) Pub Date : 2024-06-12 Rayyan Khan, Fei Gao, Kashif Khan, Muhammad Ali Shah, Haseeb Ahmad, Zhu Peng Fan, Xun Bo Zhou
Salt stress presents a major obstacle to maize (Zea mays L.) production globally, impeding its growth and development. In this study, we aimed to identify salt-tolerant maize varieties through evaluation using multivariate analysis and shed light on the role of ionome, antioxidant capacity, and autophagy in salt tolerance. We investigated multiple growth indices, including shoot fresh weight, shoot
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Shoot hydraulic impairments induced by root waterlogging: parallels and contrasts with drought Plant Physiol. (IF 6.5) Pub Date : 2024-06-12 Eduardo J Haverroth, Cristiane J Da-Silva, Matthew Taggart, Leonardo A Oliveira, Amanda A Cardoso
Soil waterlogging and drought correspond to contrasting water extremes resulting in plant dehydration. Dehydration in response to waterlogging occurs due to impairments to root water transport, but no previous study has addressed whether limitations to water transport occur beyond this organ or whether dehydration alone can explain shoot impairments. Using common bean (Phaseolus vulgaris) as a model
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Epigenetic control during root development and symbiosis Plant Physiol. (IF 6.5) Pub Date : 2024-06-12 María Eugenia Zanetti, Flavio Blanco, Milagros Ferrari, Federico Ariel, Matthias Benoit, Andreas Niebel, Martin Crespi
Summary The roots of plants play multiples functions that are essential for growth and development, including anchoring to the soil and water and nutrient acquisition. These underground organs exhibit the plasticity to modify their root system architecture in response to environmental cues allowing adaptation to change in water and nutrient availability. In addition, roots enter in mutualistic interactions
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Of attachment and connection: Auxin signaling in the cambium promotes successful plant grafting Plant Physiol. (IF 6.5) Pub Date : 2024-06-12 Janlo M Robil
Auxin signaling in the procambium is required for tissue attachment and vascular connection during graft formation.
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Reaction-diffusion modeling provides insights into biophysical carbon concentrating mechanisms in land plants Plant Physiol. (IF 6.5) Pub Date : 2024-06-10 Joshua A M Kaste, Berkley J Walker, Yair Shachar-Hill
Carbon concentrating mechanisms (CCMs) have evolved numerous times in photosynthetic organisms. They elevate the concentration of CO2 around the carbon-fixing enzyme rubisco, thereby increasing CO2 assimilatory flux and reducing photorespiration. Biophysical CCMs, like the pyrenoid-based CCM of Chlamydomonas reinhardtii or carboxysome systems of cyanobacteria, are common in aquatic photosynthetic microbes
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Dissociation of transcription factor MYB94 and histone deacetylases HDA907/908 alleviates oxidative damage in poplar Plant Physiol. (IF 6.5) Pub Date : 2024-06-08 Xiangge Kong, Yao Chen, Huanhuan Li, Menghan Li, Xuejiao Liu, Linchao Xia, Sheng Zhang
Drought is one of the major threats to forest productivity. Oxidation stress is common in drought-stressed plants, and plants need to maintain normal life activities through complex reactive oxygen scavenging mechanisms. However, the molecular links between epigenetics, oxidation stress, and drought in poplar (Populus) remain poorly understood. Here, we found that Populus plants overexpressing PtrMYB94
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Tubulin participates in establishing protoxylem vessel reinforcement patterns and hydraulic conductivity in maize Plant Physiol. (IF 6.5) Pub Date : 2024-06-08 Shiquan Huang, Siyi Guo, Liufeng Dai, Lingyu Mi, Wenrao Li, Jingjing Xing, Zhubing Hu, Wenqiang Wu, Zhikun Duan, Baozhu Li, Ting Sun, Baojie Wang, Yi Zhang, Tiqiao Xiao, Yanling Xue, Ning Tang, Han Li, Changqing Zhang, Chun-Peng Song
Water transportation to developing tissues relies on the structure and function of plant xylem cells. Plant microtubules govern the direction of cellulose microfibrils and guide secondary cell wall formation and morphogenesis. However, the relevance of microtubule-determined xylem wall thickening patterns in plant hydraulic conductivity remains unclear. In the present study, we identified a maize (Zea
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A COBRA family protein, PtrCOB3, contributes to gelatinous layer formation of tension wood fibers in poplar Plant Physiol. (IF 6.5) Pub Date : 2024-06-08 Wenjing Xu, Hao Cheng, Jiyao Cheng, Siran Zhu, Yongyao Cui, Chong Wang, Jianzhen Wu, Xingguo Lan, Yuxiang Cheng
Angiosperm trees usually develop tension wood (TW) in response to gravitational stimulation. TW comprises abundant gelatinous (G-) fibers with thick G-layers primarily composed of crystalline cellulose. Understanding of the pivotal factors governing G-layer formation in TW fiber remains elusive. This study elucidates the role of a Populus trichocarpa COBRA family protein, PtrCOB3, in the G-layer formation
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Absence of alka(e)nes triggers profound remodeling of glycerolipid and carotenoid composition in cyanobacteria membrane Plant Physiol. (IF 6.5) Pub Date : 2024-06-08 Rui Miao, Bertrand Légeret, Stéphan Cuine, Adrien Burlacot, Peter Lindblad, Yonghua Li-Beisson, Fred Beisson, Gilles Peltier
Alka(e)nes are produced by many living organisms and exhibit diverse physiological roles, reflecting a high functional versatility. Alka(e)nes serve as waterproof wax in plants, communicating pheromones for insects, and microbial signaling molecules in some bacteria. Although alka(e)nes have been found in cyanobacteria and algal chloroplasts, their importance for photosynthetic membranes has remained
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CDPK5 and CDPK13 play key roles in acclimation to low oxygen through the control of RBOH-mediated ROS production in rice Plant Physiol. (IF 6.5) Pub Date : 2024-06-08 Jingxia Li, Takahiro Ishii, Miki Yoshioka, Yuta Hino, Mika Nomoto, Yasuomi Tada, Hirofumi Yoshioka, Hirokazu Takahashi, Takaki Yamauchi, Mikio Nakazono
CALCIUM-DEPENDENT PROTEIN KINASE (CDPK) stimulates reactive oxygen species (ROS)-dependent signaling by activating RESPIRATORY BURST OXIDASE HOMOLOG (RBOH). The lysigenous aerenchyma is a gas space created by cortical cell death that facilitates oxygen diffusion from the shoot to the root tips. Previously, we showed that RBOHH is indispensable for the induction of aerenchyma formation in rice (Oryza
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A network comprising ELONGATED HYPOCOTYL5, microRNA397b, and auxin-associated factors regulates root hair growth in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2024-05-31 Subhash Reddy Gaddam, Ashish Sharma, Chitra Bhatia, Prabodh Kumar Trivedi
ELONGATED HYPOCOTYL 5 (HY5) is a major light-associated transcription factor involved in plant growth and development. In Arabidopsis (Arabidopsis thaliana), the role of HY5 is very well-defined in regulating primary root growth and lateral root formation; however, information regarding its role in root hair development is still lacking, and little is known about the genetic pathways regulating this
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Natural variation in LONELY GUY-Like 1 regulates rice grain weight under warmer night conditions Plant Physiol. (IF 6.5) Pub Date : 2024-05-31 Jaspreet Sandhu, Larissa Irvin, Anil Kumar Chandaran, Shohei Oguro, Puneet Paul, Balpreet Dhatt, Waseem Hussain, Shannon S Cunningham, Cherryl O Quinones, Argelia Lorence, Maria Arlene Adviento-Borbe, Paul Staswick, Gota Morota, Harkamal Walia
Global nighttime temperatures are rising at twice the rate of daytime temperatures and pose a challenge for rice (Oryza sativa) production. High nighttime temperature (HNT) stress affects rice yield by reducing grain weight, size, and fertility. Although the genes associated with these yield parameters have been identified and characterized under normal temperatures, the genetic basis of grain weight
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NCPbook: a comprehensive database of non-canonical peptides Plant Physiol. (IF 6.5) Pub Date : 2024-05-29 Abdul Sami, Mengjia Fu, Haoqiang Yin, Usman Ali, Lei Tian, Shunxi Wang, Jinghua Zhang, Xueyan Chen, Hehuan Li, Minghui Chen, Wen Yao, Liuji Wu
Non-canonical peptides (NCPs) are a class of peptides generated from regions previously thought of as non-coding, such as introns, 5' untranslated regions (UTRs), 3' UTRs, and intergenic regions. In recent years, the significance and diverse functions of NCPs have come to light, yet a systematic and comprehensive NCP database remains absent. Here, we developed NCPbook (https://ncp.wiki/ncpbook/), a
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The white lupin trehalase gene LaTRE1 regulates cluster root formation and function under phosphorus deficiency Plant Physiol. (IF 6.5) Pub Date : 2024-05-28 Tianyu Xia, Xiaoqi Zhu, Yujie Zhan, Bowen Liu, Xiangxue Zhou, Qian Zhang, Weifeng Xu
Under phosphorus (P) deficiency, white lupin (Lupinus albus L.) forms specialized root structure, called cluster root (CR), to improve soil exploration and nutrient acquisition. Sugar signaling is thought to play a vital role in the development of CR. Trehalose and its associated metabolites are the essential sugar signal molecules that link growth and development to carbon metabolism in plants, however
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Alternative localization of HEME OXYGENASE 1 in plant cells regulates cytosolic heme catabolism Plant Physiol. (IF 6.5) Pub Date : 2024-05-28 Yingxi Chen, Kohji Nishimura, Mutsutomo Tokizawa, Yoshiharu Y Yamamoto, Yoshito Oka, Tomonao Matsushita, Kousuke Hanada, Kazumasa Shirai, Shoji Mano, Takayuki Shimizu, Tatsuru Masuda
Heme, an organometallic tetrapyrrole, is widely engaged in oxygen transport, electron delivery, enzymatic reactions, and signal transduction. In plants, it is also involved in photomorphogenesis and photosynthesis. HEME OXYGENASE 1 (HO1) initiates the first committed step in heme catabolism, and it has generally been thought that this reaction takes place in chloroplasts. Here, we show that HO1 in
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Delta-5 elongase knockout reduces docosahexaenoic acid and lipid synthesis and increases heat sensitivity in a diatom Plant Physiol. (IF 6.5) Pub Date : 2024-05-26 Junkai Zhu, Shuangqing Li, Weizhong Chen, Xinde Xu, Xiaoping Wang, Xinwei Wang, Jichang Han, Juliette Jouhet, Alberto Amato, Eric Maréchal, Hanhua Hu, Andrew E Allen, Yangmin Gong, Haibo Jiang
Recent global marine lipidomic analysis reveals a strong relationship between ocean temperature and phytoplanktonic abundance of omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are essential for human nutrition and primarily sourced from phytoplankton in marine food webs. In phytoplanktonic organisms, EPA may play
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An insertion in the promoter of a malate dehydrogenase gene regulates malic acid content in apple fruit Plant Physiol. (IF 6.5) Pub Date : 2024-05-24 Meng Gao, Nanxiang Yang, Yingli Shao, Tian Shen, Wenxin Li, Baiquan Ma, Xiaoyu Wei, Yong-Ling Ruan, Fengwang Ma, Mingjun Li
Malic acid is an important flavor determinant in apple (Malus domestica Borkh.) fruit. One known variation controlling malic acid is the A/G SNP in an aluminium-activated malate transporter gene (MdMa1). Nevertheless, there are still differences in malic acid content in apple varieties with the same Ma1 genotype (Ma1/Ma1 homozygous), such as ‘Honeycrisp’ (high malic acid content) and ‘Qinguan’ (low
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Plasticity of the Arabidopsis leaf lipidome and proteome in response to pathogen infection and heat stress Plant Physiol. (IF 6.5) Pub Date : 2024-05-23 Patricia Scholz, Nathan M Doner, Katharina Gutbrod, Cornelia Herrfurth, Philipp Niemeyer, Magdiel S S Lim, Katharina Blersch, Kerstin Schmitt, Oliver Valerius, John Shanklin, Ivo DOE Feussner, Peter Dörmann, Gerhard H Braus, Robert T Mullen, Till Ischebeck
Plants must cope with a variety of stressors during their life cycle, and the adaptive responses to these environmental cues involve all cellular organelles. Among them, comparatively little is known about the contribution of cytosolic lipid droplets (LDs) and their core set of neutral lipids and associated surface proteins to the rewiring of cellular processes in response to stress. Here, we analyzed
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ALTERED MERISTEM PROGRAM1 sustains cellular differentiation by limiting HD-ZIP III transcription factor gene expression Plant Physiol. (IF 6.5) Pub Date : 2024-05-23 Saiqi Yang, Olena Poretska, Brigitte Poppenberger, Tobias Sieberer
Plants show remarkable developmental and regenerative plasticity through the sustained activity of stem cells in meristems. Under certain conditions, pluripotency can even be re-established in cells that have already entered differentiation. Mutation of the putative carboxypeptidase ALTERED MERISTEM PROGRAM1 (AMP1) in Arabidopsis (Arabidopsis thaliana) causes a set of hypertrophic phenotypes, indicating
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Endoplasmic reticulum protein ALTERED MERISTEM PROGRAM 1 negatively regulates senescence in Arabidopsis Plant Physiol. (IF 6.5) Pub Date : 2024-05-23 Hui Xue, Wenhui Zhou, Lan Yang, Shuting Li, Pei Lei, Xue An, Min Jia, Hongchang Zhang, Fei Yu, Jingjing Meng, Xiayan Liu
Plant senescence is a highly regulated developmental program crucial for nutrient reallocation and stress adaptation in response to developmental and environmental cues. Stress-induced and age-dependent natural senescence share both overlapping and distinct molecular responses and regulatory schemes. Previously, we have utilized a carbon-deprivation (C-deprivation) senescence assay using Arabidopsis
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Symplasmic phloem loading and subcellular transport in storage roots are key factors for carbon allocation in cassava Plant Physiol. (IF 6.5) Pub Date : 2024-05-22 David Rüscher, Viktoriya V Vasina, Jan Knoblauch, Leo Bellin, Benjamin Pommerrenig, Saleh Alseekh, Alisdair R Fernie, H Ekkehard Neuhaus, Michael Knoblauch, Uwe Sonnewald, Wolfgang Zierer
Cassava (Manihot esculenta) is a deciduous woody perennial shrub that stores large amounts of carbon and water in its storage roots. Previous studies have shown that assimilate unloading into storage roots happens symplasmically once secondary anatomy is established. However, mechanisms controlling phloem loading and overall carbon partitioning to different cassava tissues remain unclear. Here, we
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Pinpointing the causal influences of stomatal anatomy and behavior on minimum, operational, and maximum leaf surface conductance Plant Physiol. (IF 6.5) Pub Date : 2024-05-22 Marissa E Ochoa, Christian Henry, Grace John, Camila Medeiros, Ruihua Pan, Christine Scoffoni, Thomas N Buckley, Lawren Sack
Leaf surface conductance to water vapor and CO2 across the epidermis (gleaf) strongly determines rates of gas exchange. Thus, clarifying the drivers of gleaf has important implications for resolving mechanisms of photosynthetic productivity and leaf and plant responses and tolerance to drought. It is well recognized that gleaf is a function of the conductances of the stomata (gs) and of the epidermis
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Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid Plant Physiol. (IF 6.5) Pub Date : 2024-05-21 Santeri Kankaanpää, Enni Väisänen, Geert Goeminne, Rabah Soliymani, Sandrien Desmet, Anatoliy Samoylenko, Seppo Vainio, Gunnar Wingsle, Wout Boerjan, Ruben Vanholme, Anna Kärkönen
Lignin is a phenolic polymer in plants that rigidifies the cell walls of water-conducting tracheary elements and support-providing fibers and stone cells. Different mechanisms have been suggested for the transport of lignin precursors to the site of lignification in the cell wall. Extracellular vesicle (EV)-enriched samples isolated from a lignin-forming cell suspension culture of Norway spruce (Picea
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A Genetically Encoded Fluorescent Heme Sensor Detects Free Heme in Plants Plant Physiol. (IF 6.5) Pub Date : 2024-05-19 Bingxiao Wen, Bernhard Grimm
Heme is produced in plants via a plastid-localized metabolic pathway and is subsequently distributed to all cellular compartments. In addition to covalently and non-covalently bound heme, a comparatively small amount of free heme that is not associated with protein is available for incorporation into heme-dependent proteins in all subcellular compartments and for regulatory purposes. This “labile”
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Low nitrate under waterlogging triggers exodermal suberization to form a barrier to radial oxygen loss in rice roots Plant Physiol. (IF 6.5) Pub Date : 2024-05-18 Katsuhiro Shiono, Masato Ejiri, Yuto Sawazaki, Yuka Egishi, Tomonori Tsunoda
To acclimate to hypoxic waterlogged conditions, the roots of wetland plants form a radial oxygen loss (ROL) barrier that can promote oxygen diffusion to the root tips. We hypothesized that the low nitrate concentrations that occur after molecular oxygen is consumed in waterlogged soils are an environmental trigger for ROL barrier formation in rice (Oryza sativa). We previously identified 128 tissue-specific
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Tissue-specific deposition, speciation and transport of antimony in rice Plant Physiol. (IF 6.5) Pub Date : 2024-05-18 Hengliang Huang, Naoki Yamaji, Jian Feng Ma
Rice (Oryza sativa) as a staple food is a potential intake source of antimony (Sb), a toxic metalloid. However, how rice accumulates this element is still poorly understood. Here, we investigated tissue-specific deposition, speciation, and transport of Sb in rice. We found that Sb(III) is the preferential form of Sb uptake in rice, but most Sb accumulates in the roots, resulting in a very low root-to-shoot
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The genome of Lespedeza potaninii reveals biased subgenome evolution and drought adaptation Plant Physiol. (IF 6.5) Pub Date : 2024-05-17 Qi Yan, Pan Xu, Yunyue Xiao, Lijun Chen, Fan Wu, Shengsheng Wang, Fukang Guo, Zhen Duan, Jiyu Zhang
Lespedeza potaninii, a xerophytic subshrub belonging to the legume family, is native to the Tengger Desert and is highly adapted to drought. It has important ecological value due to its drought adaptability, but the underlying molecular mechanisms remain largely unknown. Here, we report a 1.24 Gb chromosome-scale assembly of the L. potaninii genome (contig N50=15.75 Mb). Our results indicate that L
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In vivo X-ray microtomography locally affects stem radial growth with no immediate physiological impact Plant Physiol. (IF 6.5) Pub Date : 2024-05-17 Laura Mekarni, Hervé Cochard, Marco M Lehmann, Pascal Turberg, Charlotte Grossiord
Micro-computed tomography (µCT) is a non-destructive X-ray imaging method used in plant physiology to visualize in-situ plant tissues that enables assessments of embolized xylem vessels. Whereas evidence for X-ray-induced cellular damage has been reported, the impact on plant physiological processes such as carbon (C) uptake, transport, and use are unknown. Yet, these damages could be particularly
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Transcriptional factor MdESE3 controls fruit acidity by activating genes regulating malic acid content in apple Plant Physiol. (IF 6.5) Pub Date : 2024-05-17 Litong Zheng, Wenfang Ma, Peipei Liu, Shujie Song, Liang Wang, Wei Yang, Hang Ren, Xiaoyu Wei, Lingcheng Zhu, Jiaqing Peng, Fengwang Ma, Mingjun Li, Baiquan Ma
Acidity is a key factor controlling fruit flavor and quality. In a previous study, combined transcriptome and methylation analyses identified a P3A-type ATPase from apple (Malus domestica), MdMa11, which regulates vacuolar pH when expressed in Nicotiana benthamiana leaves. In this study, the role of MdMa11 in controlling fruit acidity was verified in apple calli, fruits, and plantlets. In addition
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Effector Cs02526 from Ciboria shiraiana induces cell death and modulates plant immunity Plant Physiol. (IF 6.5) Pub Date : 2024-05-16 Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Shuman Liu, Panpan Zhu, Xiaohui Gu, Shuchang Wang, Aichun Zhao
Sclerotinia disease is one of the most devastating fungal diseases worldwide, as it reduces the yields of many economically important crops. Pathogen-secreted effectors play crucial roles in infection processes. However, key effectors of Ciboria shiraiana, the pathogen primarily responsible for sclerotinia disease in mulberry (Morus spp.), remain poorly understood. In this study, we identified and