Conditional protein-protein interactions enable dynamic regulation of cellular activity and are an attractive approach to probe native protein interactions, improve metabolic engineering of microbial factories, and develop smart therapeutics. Conditional protein-protein interactions have been engineered to respond to various chemical, light, and nucleic acid-based stimuli. These interactions have been applied to assemble protein fragments, build protein scaffolds, and spatially organize proteins in many microbial and higher-order hosts. To foster the development of novel conditional protein-protein interactions that respond to new inputs or can be utilized in alternative settings, we provide an overview of the process of designing new engineered protein interactions while showcasing many recently developed computational tools that may accelerate protein engineering in this space.

中文翻译：

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设计条件性蛋白质-蛋白质相互作用以实现动态细胞控制


条件性蛋白质-蛋白质相互作用能够对细胞活动进行动态调节，是探测天然蛋白质相互作用、改善微生物工厂的代谢工程和开发智能疗法的一种有吸引力的方法。条件性蛋白质-蛋白质相互作用已被设计为响应各种化学、光和基于核酸的刺激。这些相互作用已被应用于组装蛋白质片段、构建蛋白质支架以及在许多微生物和高阶宿主中对蛋白质进行空间组织。为了促进响应新输入或可用于替代环境的新型条件性蛋白质-蛋白质相互作用的发展，我们概述了设计新的工程蛋白质相互作用的过程，同时展示了许多最近开发的计算工具，这些工具可能会加速该领域的蛋白质工程。