Pterostilbene (PST), a 3’,5’-O-methylated derivative of resveratrol (RSV), is a potent natural antioxidant produced by some plants in trace amounts as defense compound. It exhibits various health-promoting activities, such as anticancer, antiviral, and antimicrobial effects. Large-scale biosynthesis of PST is crucial due to the challenges associated with extracting it from plants. This study aims to develop an efficient method for PST production using an engineered Escherichia coli strain by feeding RSV as a precursor. We introduced a two-step substrate addition strategy combined with immobilized RSV (IMRSV) on macroporous adsorption resin (MAR) to enhance PST production. Five MARs were selected for RSV immobilization, and the substrate addition strategy and fermentation parameters for PST synthesis were optimized. A maximum PST concentration of 403 ± 9 mg/L was achieved, representing a 239% increase over the control, which in a one-step addition of free RSV. The PST titer reached 395 ± 24 mg/L in a 3-L bioreactor. In conclusion, the combination of a two-step substrate addition system and IMRSV is a promising approach for the economical and industrial-scale production of PST.

中文翻译：

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使用两步底物添加策略在大孔吸附树脂上从白藜芦醇生物合成大肠杆菌中的紫檀芪


紫檀芪 （PST） 是白藜芦醇 （RSV） 的 3'，5'-O-甲基化衍生物，是一种有效的天然抗氧化剂，由一些植物以微量产生作为防御化合物。它表现出各种促进健康的活性，例如抗癌、抗病毒和抗菌作用。由于从植物中提取 PST 存在相关挑战，因此 PST 的大规模生物合成至关重要。本研究旨在通过喂养 RSV 作为前体，使用工程化的大肠杆菌菌株开发一种有效的 PST 生产方法。我们引入了一种两步底物添加策略，结合大孔吸附树脂 （MAR） 上的固定化 RSV （IMRSV） 以提高 PST 的产生。选择 5 个 MARs 用于 RSV 固定化，并优化 PST 合成的底物添加策略和发酵参数。最大 PST 浓度达到 403 ± 9 mg/L，比对照增加了 239%，这是一步添加游离 RSV。在 3 L 生物反应器中，PST 滴度达到 395 ± 24 mg/L。总之，两步底物添加系统和 IMRSV 的结合是经济和工业规模生产 PST 的一种很有前途的方法。