Quantification of cyanobacterial CO2 fixation rates is vital to determining their potential as industrial strains in a circular bioeconomy. Currently, however, CO2 fixation rates are most often determined through indirect and/or low‐resolution methods, resulting in an incomplete picture of both dynamic behaviors and total carbon fixation potential. To address this, we developed the “Automated Carbon and CO2 Experimental Sampling System” (ACCESS); a low‐cost system for in situ off‐gas analysis that supports the automated acquisition of high‐resolution volumetric CO2 uptake rates from multiple cyanobacterial cultures in parallel. Carbon fixation data obtained via ACCESS were first independently validated by elemental analysis of cultivated biomass. Using ACCESS, we then demonstrate how the volumetric CO2 uptake rate of two model cyanobacteria, Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803, accelerates linearly to a maximum before then decaying monotonically to cessation by stationary phase. Furthermore, consistent with the expected stoichiometry, strong correlations were also found to exist between cell growth and carbon fixation, both in terms of rates as well as total levels. The novel insights made possible via ACCESS will aid other cyanobacterial researchers in diverse fundamental and applied research efforts.

中文翻译：

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通过自动废气分析对 CO2 吸收率进行原位高分辨率定量分析，阐明蓝藻培养物中的碳吸收动力学


蓝藻 CO₂ 固定率的定量对于确定它们在循环生物经济中作为工业菌株的潜力至关重要。然而，目前，CO₂ 固定率通常是通过间接和/或低分辨率方法确定的，导致动态行为和总碳固定潜力的图片不完整。为了解决这个问题，我们开发了“自动碳和 CO₂ 实验采样系统”（ACCESS）;一种用于原位废气分析的低成本系统，支持从多个蓝藻培养物中并行自动采集高分辨率体积 CO₂ 吸收率。通过 ACCESS 获得的碳固定数据首先通过培养生物质的元素分析进行独立验证。然后使用 ACCESS，我们展示了两种模型蓝细菌 Synechococcus sp 的体积 CO₂ 吸收率。PCC 7002 和 集胞藻属PCC 6803 线性加速至最大值，然后单调衰减至固定相停止。此外，与预期的化学计量一致，还发现细胞生长和碳固定之间存在很强的相关性，无论是在速率还是总水平方面。通过 ACCESS 实现的新见解将帮助其他蓝藻研究人员进行各种基础和应用研究工作。