Vascularization is a key issue facing the construction of functional three‐dimensional (3D) tissues, which is critical for the long‐term survival and stability of tissue construct transplantation. In this study, a photocurable hydrogel material carboxymethyl chitosan (CHIMA) was successfully prepared and integrated with methacryloyl gelatin (GelMA) to construct the bioink GelMA/CHIMA, which was subsequently used 3D printing technology to prepared a bioactive scaffold with angiogenesis‐inducing functionality. The results showed that the cross‐linked GelMA/CHIMA bioink had a porous structure that supported cell growth and metabolism. The incorporation of CHIMA could significantly improve the hydrophilicity, swelling rate, pressure resistance and mechanical strength of the bioink. GelMA/CHIMA bioink supported the survival and continued proliferation of human umbilical vein endothelial cells (HUVECs) in the scaffold. In particular, the bioink composed of 8 wt% GelMA and 2 wt% CHIMA could stimulate the expression of angiogenesis genes. 3D printed bioactive scaffolds supported the survival of HUVECs and had abundant protein deposition including CD31 and VEGF. Therefore, this study constructed a bioactive scaffold with angiogenesis induction function, which provides a feasible strategy for the construction of vascularized complex tissues.

中文翻译：

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用于血管生成的 3D 打印 GelMA/CHIMA 交联网络水凝胶


血管形成是功能性三维 （3D） 组织构建面临的一个关键问题，这对于组织结构移植的长期生存和稳定性至关重要。在这项研究中，成功制备了一种光固化水凝胶材料羧甲基壳聚糖 （CHIMA），并与甲基丙烯酰明胶 （GelMA） 整合构建了生物墨水 GelMA/CHIMA，随后使用 3D 打印技术制备了具有血管生成诱导功能的生物活性支架。结果表明，交联的 GelMA/CHIMA 生物墨水具有支持细胞生长和新陈代谢的多孔结构。CHIMA 的掺入可以显着提高生物墨水的亲水性、溶胀率、耐压性和机械强度。GelMA/CHIMA 生物墨水支持支架中人脐静脉内皮细胞 （HUVEC） 的存活和持续增殖。特别是，由 8 wt% GelMA 和 2 wt% CHIMA 组成的生物墨水可以刺激血管生成基因的表达。3D 打印的生物活性支架支持 HUVEC 的存活，并具有丰富的蛋白质沉积，包括 CD31 和 VEGF。因此，本研究构建了具有血管生成诱导功能的生物活性支架，为血管化复杂组织的构建提供了可行的策略。