Culture system for longitudinal monitoring of bone dynamics ex vivo,Biotechnology and Bioengineering

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Culture system for longitudinal monitoring of bone dynamics ex vivo
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2024-09-19 , DOI: 10.1002/bit.28848
Esther E A Cramer _{1,

2} , Kim C J Hermsen _{1,

2} , Linda M Kock _{1,

3} , Keita Ito _{1,

2} , Sandra Hofmann _{1,

2}

Affiliation

To quantify and visualize both bone formation and resorption within osteochondral explants cultured ex vivo is challenging with the current analysis techniques. An approach that enables monitoring of bone remodeling dynamics is longitudinal microcomputed tomography (µCT), a non‐destructive technique that relies on repeated µCT scanning and subsequent registration of consecutive scans. In this study, a two‐compartment culture system suitable for osteochondral explants that allowed for µCT scanning during ex vivo culture was established. Explants were scanned repeatedly in a fixed orientation, which allowed assessment of bone remodeling due to adequate image registration. Using this method, bone formation was found to be restricted to the outer surfaces when cultured statically. To demonstrate that the culture system could capture differences in bone remodeling, explants were cultured statically and under dynamic compression as loading promotes osteogenesis. No quantitative differences between static and dynamic culture were revealed. Still, only in dynamic conditions, bone formation was visualized on trabecular surfaces located within the inner cores, suggesting enhanced bone formation towards the center of the explants upon mechanical loading. Taken together, the ex vivo culture system in combination with longitudinal µCT scanning and subsequent registration of images demonstrated potential for evaluating bone remodeling within explants.

中文翻译：

用于离体骨动力学纵向监测的培养系统

目前的分析技术对离体培养的骨软骨外植体中的骨形成和骨吸收进行量化和可视化具有挑战性。纵向微计算机断层扫描（μCT）是一种能够监测骨重塑动态的方法，这是一种非破坏性技术，依赖于重复的μCT扫描和后续连续扫描的注册。在这项研究中，建立了适合骨软骨外植体的双室培养系统，允许在离体培养期间进行μCT扫描。以固定方向重复扫描外植体，通过充分的图像配准来评估骨重塑。使用这种方法，发现静态培养时骨形成仅限于外表面。为了证明培养系统可以捕捉骨重塑的差异，在负载促进成骨时静态和动态压缩下培养外植体。静态和动态培养之间没有发现定量差异。尽管如此，只有在动态条件下，位于内核内的小梁表面上才能看到骨形成，这表明在机械负载下朝向外植体中心的骨形成增强。总而言之，离体培养系统与纵向 µCT 扫描和随后的图像配准相结合，证明了评估外植体内骨重塑的潜力。

更新日期：2024-09-19

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