The SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin remodeling complex is involved in various aspects of plant development and stress responses. Here, we investigated the role of BRM (BRAHMA), a core catalytic subunit of the SWI/SNF complex, in Arabidopsis thaliana seed biology. brm-3 seeds exhibited enlarged size, reduced yield, increased longevity, and enhanced secondary dormancy, but did not show changes in primary dormancy or salt tolerance. Some of these phenotypes depended on the expression of DOG1, a key regulator of seed dormancy, as they were restored in the brm-3 dog1-4 double mutant. Transcriptomic and metabolomic analyses revealed that BRM and DOG1 synergistically modulate the expression of numerous genes. Some of the changes observed in the brm-3 mutant, including increased glutathione levels, depended on a functional DOG1. We demonstrated that the BRM-containing chromatin remodeling complex directly controls secondary dormancy through DOG1 by binding and remodeling its 3’ region, where the promoter of the long non-coding RNA asDOG1 is located. Our results suggest that BRM and DOG1 cooperate to control seed physiological properties and that BRM regulates DOG1 expression through asDOG1. This study reveals chromatin remodeling at the DOG1 locus as a molecular mechanism controlling the interplay between seed viability and dormancy.

中文翻译：

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BRAHMA 相关的 SWI/SNF 染色质重塑复合物控制拟南芥种子的质量和生理学


SWI/SNF （SWItch/Sucrose Non-Fermentable） 染色质重塑复合物参与植物发育和胁迫反应的各个方面。在这里，我们研究了 BRM （BRAHMA） 的作用，BRM 是 SWI/SNF 复合物的核心催化亚基，在拟南芥种子生物学中的作用。BRM-3 种子表现出体积增大、产量降低、寿命延长和次生休眠增强，但未表现出初级休眠或耐盐性的变化。其中一些表型依赖于种子休眠的关键调节因子 DOG1 的表达，因为它们在 brm-3 dog1-4 双突变体中恢复。转录组学和代谢组学分析显示，BRM 和 DOG1 协同调节许多基因的表达。在 brm-3 突变体中观察到的一些变化，包括谷胱甘肽水平的增加，都依赖于功能性 DOG1。我们证明，含有 BRM 的染色质重塑复合物通过结合和重塑其 3' 区域（长链非编码 RNA asDOG1 的启动子所在的区域）直接通过 DOG1 控制次级休眠。我们的结果表明，BRM 和 DOG1 合作控制种子生理特性，并且 BRM 通过 asDOG1 调节 DOG1 表达。本研究揭示了 DOG1 位点的染色质重塑是控制种子活力和休眠之间相互作用的分子机制。