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Histone modification H3K27me3 is essential during chilling-induced flowering in Litchi chinensis
Plant Physiology ( IF 6.5 ) Pub Date : 2024-11-20 , DOI: 10.1093/plphys/kiae619
Xifen Pan, Xingyu Lu, Lijie Huang, Zhiqun Hu, Maogen Zhuo, Yanchun Ji, Bingqi Lin, Jianqin Luo, Peitao Lü, Biyan Zhou
Plant Physiology ( IF 6.5 ) Pub Date : 2024-11-20 , DOI: 10.1093/plphys/kiae619
Xifen Pan, Xingyu Lu, Lijie Huang, Zhiqun Hu, Maogen Zhuo, Yanchun Ji, Bingqi Lin, Jianqin Luo, Peitao Lü, Biyan Zhou
Litchi (Litchi chinensis), a prominent fruit tree in the Sapindaceae, initiates flowering in response to low autumn and winter temperatures. This study investigates the epigenetic regulation of this process, focusing on the marks histone H3 lysine 27 trimethylation (H3K27me3) and its deposition genes during the chilling-induced floral induction (FId) and initiation stages. Our genomic analysis delineated the H3K27me3 deposition landscape across the prefloral induction (PFId), FId, and floral initiation (FIn) stages. We identified 5,635 differentially H3K27me3-modified genes (DHGs) in buds and 4,801 DHGs in leaves. Integration of the RNA-seq and ChIP-seq datasets identified 1,001 differentially regulated genes (DRGs) in buds and 675 DRGs in leaves, offering insights into the genes potentially targeted by H3K27me3. To probe the functional role of H3K27me3, we employed GSK343, a histone H3 lysine methyltransferase inhibitor. Treatment with GSK343 during the chilling-induced flowering process led to reduced H3K27me3 deposition at the TREHALOSE-6-PHOSPHATE SYNTHASE 1 (LcTPS1) and FRIGIDA (LcFRI) loci, resulting in increased gene expression. This manipulation delayed flowering and reduced flowering rates, confirming the pivotal role of H3K27me3 in chilling-induced flowering in litchi. Gene coexpression network analysis identified SHORT VEGETATIVE PHASE 10 (LcSVP10) as a crucial regulator in litchi flowering. Overexpression of LcSVP10 in Arabidopsis thaliana delayed flowering, indicating a conserved function in flowering time control. Our results elucidate the molecular and epigenetic mechanisms that govern FId in litchi and highlight the potential of epigenetic modifications to regulate flowering time in horticultural plants.
中文翻译:
组蛋白修饰 H3K27me3 在荔枝中低温诱导的开花中是必不可少的
荔枝 (Litchi chinensis) 是无患子科中的重要果树,在秋冬季温较低时开始开花。本研究探讨了这一过程的表观遗传调控,重点关注组蛋白 H3 赖氨酸 27 三甲基化 (H3K27me3) 及其在寒冷诱导花诱导 (FId) 和起始阶段的沉积基因。我们的基因组分析描绘了 H3K27me3 在花前诱导 (PFId)、FId 和花起始 (FIn) 阶段的沉积景观。我们在芽中鉴定了 5,635 个差异 H3K27me3 修饰基因 (DHG),在叶片中鉴定了 4,801 个 DHG。RNA-seq 和 ChIP-seq 数据集的整合在芽中确定了 1,001 个差异调控基因 (DRG),在叶中发现了 675 个 DRG,从而深入了解了 H3K27me3 可能靶向的基因。为了探究 H3K27me3 的功能作用,我们采用了组蛋白 H3 赖氨酸甲基转移酶抑制剂 GSK343。在寒冷诱导的开花过程中用 GSK343 处理导致 H3K27me3 在海藻糖-6-磷酸合酶 1 (LcTPS1) 和 FRIGIDA (LcFRI) 基因座的沉积减少,导致基因表达增加。这种操作延迟了开花并降低了开花率,证实了 H3K27me3 在荔枝寒冷诱导开花中的关键作用。基因共表达网络分析确定短营养期 10 (LcSVP10) 是荔枝开花的关键调节因子。拟南芥中过表达 LcSVP10 延迟了开花,表明在开花时间控制中具有保守功能。我们的结果阐明了控制荔枝 FId 的分子和表观遗传机制,并强调了表观遗传修饰调节园艺植物开花时间的潜力。
更新日期:2024-11-20
中文翻译:
组蛋白修饰 H3K27me3 在荔枝中低温诱导的开花中是必不可少的
荔枝 (Litchi chinensis) 是无患子科中的重要果树,在秋冬季温较低时开始开花。本研究探讨了这一过程的表观遗传调控,重点关注组蛋白 H3 赖氨酸 27 三甲基化 (H3K27me3) 及其在寒冷诱导花诱导 (FId) 和起始阶段的沉积基因。我们的基因组分析描绘了 H3K27me3 在花前诱导 (PFId)、FId 和花起始 (FIn) 阶段的沉积景观。我们在芽中鉴定了 5,635 个差异 H3K27me3 修饰基因 (DHG),在叶片中鉴定了 4,801 个 DHG。RNA-seq 和 ChIP-seq 数据集的整合在芽中确定了 1,001 个差异调控基因 (DRG),在叶中发现了 675 个 DRG,从而深入了解了 H3K27me3 可能靶向的基因。为了探究 H3K27me3 的功能作用,我们采用了组蛋白 H3 赖氨酸甲基转移酶抑制剂 GSK343。在寒冷诱导的开花过程中用 GSK343 处理导致 H3K27me3 在海藻糖-6-磷酸合酶 1 (LcTPS1) 和 FRIGIDA (LcFRI) 基因座的沉积减少,导致基因表达增加。这种操作延迟了开花并降低了开花率,证实了 H3K27me3 在荔枝寒冷诱导开花中的关键作用。基因共表达网络分析确定短营养期 10 (LcSVP10) 是荔枝开花的关键调节因子。拟南芥中过表达 LcSVP10 延迟了开花,表明在开花时间控制中具有保守功能。我们的结果阐明了控制荔枝 FId 的分子和表观遗传机制,并强调了表观遗传修饰调节园艺植物开花时间的潜力。