CELL DIVISION CYCLE 5 (CDC5) is a R2R3-type MYB transcription factor, serving as a key component of Modifier of snc1, 4 (MOS4)-associated complex (MAC)/NineTeen Complex (NTC), which is associated with plant immunity, RNA splicing, and miRNA biogenesis. In this study, we demonstrate that mutation of CDC5 accelerates flowering in Arabidopsis (Arabidopsis thaliana). CDC5 activates the expression of FLOWERING LOCUS C (FLC) by binding to and affecting the enrichment of RNA polymerase II on FLC chromatin. Moreover, genetic analysis confirmed that CDC5 regulates flowering in an FLC-dependent manner. Furthermore, we characterized the interaction of CDC5 with the RNA polymerase-associated factor 1 (Paf1) complex and confirmed that CDC5, as part of the spliceosome, mediates genome-wide alternative splicing, as revealed by RNA-Seq. CDC5 affected the splicing of flowering-associated genes such as FLC, SEF, and MAFs. Additionally, we also demonstrated that CDC5 contributes to the regulation of histone modification of FLC chromatin, which further promotes FLC expression. In summary, our results establish CDC5 as a key factor regulating flowering. This provides valuable insight for future research into plant flowering.

中文翻译：

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CELL DIVISION CYCLE 5 通过调节拟南芥中的开花基因转录和剪接来控制花的转变


细胞分裂周期 5 （CDC5） 是一种 R2R3 型 MYB 转录因子，是 snc1， 4 （MOS4） 相关复合物 （MAC）/NineTeen 复合物 （NTC） 修饰因子的关键组分，与植物免疫、RNA 剪接和 miRNA 生物合成有关。在这项研究中，我们证明了 CDC5 的突变加速了拟南芥 （Arabidopsis thaliana） 的开花。CDC5 通过结合并影响 FLC 染色质上 RNA 聚合酶 II 的富集来激活 FLOWERING LOCUS C （FLC） 的表达。此外，遗传分析证实 CDC5 以 FLC 依赖性方式调节开花。此外，我们表征了 CDC5 与 RNA 聚合酶相关因子 1 （Paf1） 复合物的相互作用，并证实 CDC5 作为剪接体的一部分，介导全基因组选择性剪接，如 RNA-Seq 所示。CDC5 影响 FLC 、 SEF 和 MAFs 等开花相关基因的剪接。此外，我们还证明 CDC5 有助于调节 FLC 染色质的组蛋白修饰，从而进一步促进 FLC 表达。总之，我们的研究结果将 CDC5 确定为调节开花的关键因素。这为未来植物开花的研究提供了宝贵的见解。