Carbohydrate-based cell wall signaling impacts plant growth, development, and stress responses; however, how cell wall signals are perceived and transduced remains poorly understood. Several cell wall breakdown products have been described as typical damage-associated molecular patterns that activate plant immunity, including pectin-derived oligogalacturonides (OGs). Receptor kinases of the WALL-ASSOCIATED KINASE (WAK) family bind pectin and OGs, and were previously proposed as OG receptors. However, unambiguous genetic evidence for the role of WAKs in OG responses is lacking. Here, we investigated the role of Arabidopsis (Arabidopsis thaliana) WAKs in OG perception using a clustered regularly interspaced short palindromic repeats (CRISPR) mutant in which all five WAK genes were deleted. Using a combination of immune assays for early and late pattern-triggered immunity, we show that WAKs are dispensable for OG-induced signaling and immunity, indicating that they are not bona fide OG receptors.

中文翻译：

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拟南芥 WALL-ASSOCIATED KINASES 不是寡半乳糖醛酸诱导的信号传导和免疫所必需的


基于碳水化合物的细胞壁信号传导影响植物生长、发育和胁迫反应;然而，细胞壁信号是如何被感知和转导的仍然知之甚少。几种细胞壁分解产物已被描述为激活植物免疫的典型损伤相关分子模式，包括果胶衍生的寡半乳糖醛酸酯 （OG）。WALL-ASSOCIATED KINASE （WAK） 家族的受体激酶结合果胶和 OGs，以前被提议作为 OG 受体。然而，缺乏关于 WAK 在 OG 反应中的作用的明确遗传证据。在这里，我们使用成簇的规则间隔短回文重复序列 （CRISPR） 突变体研究了拟南芥 （Arabidopsis thaliana） WAK 在 OG 感知中的作用，其中所有五个 WAK 基因都被删除。使用早期和晚期模式触发免疫的免疫测定组合，我们表明 WAK 对于 OG 诱导的信号传导和免疫是可有可无的，表明它们不是真正的 OG 受体。