As an emerging contaminant, microplastics (MPs) are widely distributed in freshwater ecosystems and pose potential threats to aquatic organisms, attracting significant attention from both the scientific community and the general public. However, there is still uncertainty regarding the mechanisms of MPs transfer within aquatic biota and how particle size and food availability influence their transport

This research investigated the effects of glyphosate herbicide on golden apple snail (Pomacea canaliculata) eggs, focusing on hatching rates, morphological changes, and acetylcholinesterase (AChE) expression to explore its potential as a biomarker of exposure. Additionally, key bioactive components in golden apple snail eggs were characterized. The study demonstrated that glyphosate toxicity increased

Pollution is a major global concern affecting biodiversity, particularly of freshwater species. Populations have developed mechanisms to deal with pollution, such as the chemical defensome, which is a set of genes involved in maintaining internal stability. Pollution significantly affects the Maipo River basin in Chile. This area is home to the endemic silverside fish Basilichthys microlepidotus, whose

Bisphenol A (BPA) is a widely found endocrine-disrupting chemical (EDC). Nanoplastics (NPs) represent a novel environmental pollutant, and the combined toxicity of these pollutants on the hepatopancreas of marine arthropods is understudied. To investigate the potential risks associated with co-exposure to BPA and NPs on the hepatopancreas, Portunus trituberculatus was treated with 100 μg/L BPA, 104

Phenanthrene (PHE) is a typical polycyclic aromatic hydrocarbon compound that is ubiquitous in the environment and accumulates in aquatic products, thereby posing a risk to food safety. Oligomeric proanthocyanidins (OPC) is widely distributed powerful antioxidants with potent antioxidant and anti-inflammatory properties. This study aimed to evaluate the alleviating effects of dietary OPC on oxidative

Leptodactylus fuscus is a frog species widely distributed in the Neotropical region, occurring in several biomes, which makes it a potential biomonitor of environmental conditions. To advance the establishment of this species in this field, we developed a fibroblast-like cell line derived from the digits of the forelimbs (LFUfd) and evaluated its response to possible environmental stressors. An adult

Recent research efforts in endocrine disruption have focused on evaluating non-EATS (estrogen, androgen, thyroid, and steroidogenesis) pathways. Retinoid signaling disruption is noteworthy because of its teratogenic effects and environmental relevance. However, current environmental risk assessments are limited in their ability to evaluate impacts on individuals and populations. This study characterizes

Tadpoles, as early developmental stages of frogs, are vital indicators of toxicity and environmental health in ecosystems exposed to harmful organic compounds from industrial and runoff sources. Evaluating each compound individually is challenging, necessitating the use of in silico methods like Quantitative Structure Toxicity-Relationship (QSTR) and Quantitative Read-Across Structure-Activity Relationship

Microplastics are a novel pollutant that adversely affect freshwater benthic organisms. However, few studies have investigated the mechanism underlying the bioaccumulation and the toxicity of microplastics. In this study, microplastics bioaccumulation of wild Cipangopaludina chinensis in the Songhua River were utilized, and a 28-day aquatic toxicity test was performed to determine the effects of exposure

Chemical substances such as drugs pose a threat to the environment. One of the substances recorded in soil and water is chlortetracycline, an antibiotic used in veterinary medicine. Plants exposed to such xenobiotics show changes in the content of biogenic amines. An analytical technique - ion exchange chromatography is used to assess their content. The occurrence of these active compounds is used

Contemporary research on ichthyological behavior predominantly investigates underwater environments. However, the intricate nature of aquatic ecosystems often hampers subaqueous observations of fish behavior due to interference. Transitioning the observational perspective from subaqueous to supra-aquatic enables a more direct assessment of fish physiology and habitat conditions. In this study, we utilized

Chloroxylenol (para‑chloro-meta-xylenol, PCMX), is a widely used antimicrobial agent and can remain in the aquatic environment. Although toxicity studies related to PCMX on the aquatic animals like zebrafish and Brachionus koreanus have been reported, there are few reports in the ecological risk of amphibians. In this study, the toxicity of different concentration (143, 14.3, 1.43 μg/L) of PCMX treatments

The rising concern over heavy metals (HMs) and microplastics (MPs) pollution in marine ecosystems, primarily driven by anthropogenic activities, poses significant threats to ecological health. Understanding the combined exposure of HMs and MPs aids in toxicity assessment. In this study, we examined the combined effects of polystyrene microplastics (MPs) and iron (Fe) on oxidative stress, bioaccumulation

Ocean acidification (OA) and other environmental factors can collectively affect marine organisms. Deltamethrin (DM), a type II pyrethroid insecticide, has been widely detected in coastal and estuarine areas, while little attention has been given to the combined effects of DM and OA. In this study, Haliotis discus hannai was exposed to three pH levels (8.1, 7.7 and 7.4) and three DM nominal concentrations

After the COVID-19 pandemic, the use of quaternary ammonium compounds increased exponentially due to their efficacy as antimicrobials, stabilizers and disinfectants. Among these, Quaternium-15 is a preservative used in the formulation of a variety of personal care products. The increased use of this substance and the resulting persistence in wastewater treatment systems, which are unable to completely

Cortisone is a naturally occurring corticosteroid hormone known for its wide range of anti-inflammatory and immunosuppressive effects, and it is commonly found in various aquatic environments. Previous reports have shown that cortisone can have significant negative impacts on fish; however, its specific effects on fish reproduction have not been thoroughly investigated. In this study, female adult

The toxic effects of ammonia exposure on Litopenaeus vannamei have been widely reported, including tissue damage, oxidative stress, and metabolic disorders, but the ability of L. vannamei to recover from ammonia damage is still unclear. To further understand the adaptation mechanism of L. vannamei to ammonia, this study explored the effects of ammonia exposure and recovery on histopathology, physiological

The prevalence of organic/chemical UV-filter compounds in aquatic ecosystems represents a growing environmental issue. The long-term toxicity risks of many UV-filters at environmentally relevant concentrations to aquatic biota are still less studied, especially in the case of invertebrates. This study was designed to evaluate the chronic toxicity of avobenzone (AVO), octocrylene (OCTO), and octinoxate

Azoxystrobin, a widely used fungicide, can contaminate water bodies through surface run-off, posing a risk to aquatic organisms. This study aimed to assess the genotoxic effects of azoxystrobin on the fish Pethia conchonius. The 96 h lethal concentration (LC50) of azoxystrobin was determined to be 0.514 mg/L. Based on LC50, three sub-lethal concentrations (SLCs) of 0.025 mg/L, 0.0514 mg/L, and 0.103

Biofilms are thought to be sinks for antibiotic resistance genes (ARGs) and nanoparticles (NPs), however, studies on the interactions between NPs and ARGs in biofilms are limited. This study focused on the occurrence and regulatory mechanisms of ARGs during the formation of biofilms with continuous treatment of zero-valent silver nanoparticles (Ag0-NPs) and Ag ions at an environmental concentration

In recent years, psychoactive drugs such as benzodiazepines (BZDs) have been frequently detected in water environments, however, there is still limited understanding regarding their potential impact on neurological health and underlying mechanisms. This study evaluated the neurotoxicity of the typical BZD drug flunitrazepam (FLZ, 0.2 and 5 μg/L) in zebrafish embryos and adults, and investigated the

Microplastics (MPs) colonized by pathogens pose significant risks to the environment and health of animals and humans, however, the strategies for pathogens colonization in MPs and the effects of its colonization on spread of pathogens have not been fully characterized. Here, we investigated the biofilm formation mechanism regulated by c-di-GMP in Hafnia paralvei Z11, and determined the effect of MPs

Daphnia magna (D. magna) is a model organism widely used in aquatic ecotoxicology research due to its sensitivity to environmental changes. The survival and reproduction rates of D. magna are easily affected by toxic environments. However, their small size, fragility, and transparency, especially in neonate stages, make them challenging to count accurately. Traditionally, counting adult and neonate

As an emerging pollutant frequently detected in aquatic ecosystems, the toxicity of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine-quinone (6PPD-quinone) on fish has been confirmed, but insight into the mechanisms underlying those adverse effects is still limited. Thus, we exposed zebrafish embryos to 6PPD-quinone at 0, 0.25, 2.5, and 25 μg/L until 120 h post-fertilization (hpf), and investigated

As an alternative to perfluorooctane sulfonate, sodium p-perfluorous nonenoxybenzene sulfonate (OBS) has been widely used and caused ubiquitous water pollution. However, its toxicity to aquatic organisms is still not well known. Therefore, in this study, parental zebrafish were exposed to OBS at environmentally relevant concentrations from ∼ 2 h post-fertilization to 21 days post-fertilization (dpf)

Significant concerns have been raised by the widespread pollutants phoxim (PHO) and nanopolystyrene (NP) in the natural environment. This study evaluated the toxicity effects on the hepatopancreas of Eriocheir sinensis caused by NP and/or PHO at concentrations found in the environment. Subchronic exposure to NP and/or PHO triggered hepatopancreas histological damage within a 21-day exposure period

Silver (Ag) and cadmium (Cd) are non-essential metals that, as a result of natural processes and human activities, reach the aquatic environment where they interact with biota inducing potential toxic effects. To determine the biological effects of these metals on the endobenthic bivalve Scrobicularia plana, specimens were exposed to Ag and Cd at two concentrations, 5 and 50 μg∙L-1, for 7 days in a

Fibrous microplastics (FMPs), a unique class of microplastics, are increasingly recognized as a significant environmental threat due to their ubiquitous presence and potential risks to ecological and human health. This review provides a comprehensive overview of FMPs, including their sources, prevalence in various environmental media, and potential impacts. FMPs, which can be found in over 90 % of

Glyphosate, a commonly used herbicide, has been associated with environmental pollution and potential health risks to aquatic organisms. This study investigated the effects of glyphosate on the muscle metabolism of grass carp (Ctenopharyngodon idellus) following exposure to environmentally relevant concentrations. Over a 14-day exposure period to varying glyphosate levels, significant disruptions were

Pericardial edema – fluid accumulation within the pericardium – is a frequently observed malformation in zebrafish embryo-based chemical toxicity screens. We recently discovered that the severity of triphenyl phosphate (TPHP)-induced pericardial edema was dependent on the ionic strength of exposure media. TPHP is an aryl phosphate ester (APE) widely used as a plasticizer and flame retardant. APEs are

Glyphosate, a key ingredient in many herbicides, is increasingly present in aquatic systems due to agricultural runoff. High doses of glyphosate cause defects in organisms due to its ability to interfere with physiological processes as an endocrine disruptor. We used the mangrove rivulus fish (Kryptolebias marmoratus) to evaluate the effects of larval exposure to glyphosate on non-target species in

Tire wear particles (TWP) represent a distinctive form of microplastics (MPs) that are widely distributed in aquatic ecosystems. However, the toxicity of various types of TWP on phytoplankton remain to be further explored. Thus, three different TWPs originating from replaced bicycle, car, and electro-mobile tire (marked as BTWP, CTWP, and ETWP) were selected and their long-term biological influences

In order to estimate the effects on aquatic organisms of long-term exposure to low doses of yttrium (Y) as a potential emerging contaminant, ecotoxicological and metabolomic data were collected on the model organism Daphnia magna, a keystone species in freshwater ecosystems. Following an initial acute toxicity assessment, a 21-day chronic exposure experiment was conducted using a sublethal concentration

The microplastics (MPs) vector effect of environmental contaminants (such as polychlorinated biphenyls-PCBs) to organism tissues is currently one of the major concerns regarding MPs pollution in the marine environment. The relative importance of MPs as vectors for the bioaccumulation of contaminants to marine organisms compared to other naturally occurring particles has been poorly investigated and

Salicylate pollutants (SAs) poses a serious threat to marine ecosystems as emerging contaminants. However, the toxic effects of SAs on marine phytoplankton, as well as the potential mechanisms and their ecological risks linked with them, are remain largely unknown. In this study, we aimed to evaluate the toxic effects of salicylic acid (SA) and its 5-substituted derivatives (5-sSA) on the marine diatom

The production of nanoparticles (NPs) has recently become more prevalent owing to their numerous applications in the fast-growing nanotechnology industry. Although nanoparticles have growing applications, there is a significant concern over their environmental impact due to their inevitable release into the environment. With the increasing risk to aquatic organisms, D. magna and zebrafish (Danio rerio)

Microplastics (MPs) disturb the normal activity of aquatic organisms at different levels, causing physiological stress and altering feeding, growth, and reproduction. Alterations of epigenetic patterns due to exposure to MPs have scarcely been studied in invertebrates. In this study, Mytilus galloprovincialis mussels (N = 61) were intermittently exposed to different concentrations of pure polystyrene

The zinc oxide nanoparticles (ZnO-NPs) being widely employed in several industries and consumer products, are raising concerns about their safety on aquatic biota and human health. This study aims to investigate the possible toxicological effects of ZnO-NPs through a combined in vivo and in silico approach. Zebrafish embryos were exposed to several ZnO-NPs concentrations and morphological alterations

Diethyl phthalate (DEP) has been widely used as a plasticiser in various consumer products, including cosmetics, personal care items, and pharmaceuticals, and recent studies reported a higher abundance of this priority phthalate in the aquatic environment. DEP is a potential endocrine disruptor, affecting immune systems in humans and wildlife even at low-level chronic exposure. As concern over phthalates

The stabilization of rhizobacteria communities plays a crucial role in sustaining healthy macrophyte growth. In light of increasing evidence of combined pollution from microplastics (MPs) and per- and polyfluoroalkyl substances (PFASs), Selecting typical floating macrophyte as a case, this study explored their impacts using hydroponic simulations and 16S rRNA high-throughput sequencing. A total of

Wound healing and tissue regeneration are influenced by a variety of factors. Adverse lifestyle habits, such as excessive alcohol consumption, delay wound healing and increase the risk of secondary infections. Ethyl butyrate is a common food additive widely used to enhance the aroma of alcoholic beverages. This additive is generally considered harmless to human health in both industrial and domestic

Perfluorooctanoic acid (PFOA), which is widely used during the manufacturing of fluoropolymer coatings and polytetrafluoroethylene, is now a widespread pollutant in the environment and within the human body. This study used zebrafish, an aquatic model species, to investigate how low levels of chronic PFOA exposure affect the reproductive system. The results of the experiments in which zebrafish were

The use of in silico and in vitro methods, commonly referred to as New Approach Methodologies (NAMs), has been proposed to support environmental (and human) chemical safety decisions, ensuring enhanced environmental protection. Toxicokinetic models developed for environmentally relevant species are fundamental to the deployment of a NAMs-based safety strategy, enabling the conversion between external

Ionizing radiation, as an increasingly serious environmental pollutant, has aroused widespread public concern. Melatonin, as an indole heterocyclic compound, is known to have anti-inflammatory and antioxidant effects. However, few studies have considered the comprehensive impact of melatonin on radiation damage. In this study, we used zebrafish as experimental materials and employed methods such as

Progestins are crucial steroid hormones that have attracted wide attention due to their endocrine disrupting effects in fish. The aim of this study is to investigate the effects of long-term exposure to low concentrations of norgestrel (NGT) on the reproductive and thyroid endocrine systems of adult zebrafish. Adult zebrafish were exposed to 7 and 39 ng/L NGT for a duration of 90 days. The results

Most studies assessing the effects of microplastics (MPs) on freshwater ecosystems use reference materials of a certain size, shape, and polymer type. However, in the environment, aquatic organisms are exposed to a mixture of different polymers with different sizes and shapes, resulting in different bioaccessible fractions and effects. This study assesses the fate and effects of an environmentally

Mediterranean barbel (Barbus meridionalis) an endemic species is currently facing habitat destruction and pollution in Osor River (Spain) due to mining runoff that has severely deteriorated the water quality by metals, primarily zinc (Zn). In order to assess the potential risk of metal contamination and hydrological changes in the Osor River by using oxidative stress and neurotoxicity biomarkers via

This study investigated the effects of Bisphenol A (BPA), a common endocrine-disrupting chemical, on the antioxidant enzyme activities in the hepatopancreas and the expression of genes related to ovarian development in oriental river prawns (Macrobrachium nipponense). The 24hLC50 and 48hLC50 values for BPA were 80.59 mg/L and 63.90 mg/L, respectively, with a safe concentration of 12.06 mg/L. Prawns

The occurrence of iodinated contrast agents (ICAs) in the aquatic environment is relatively well documented, showing that these compounds can be found at several µg/L in natural waters, and up to hundreds of µg/L in waste water treatment plants inlets. Nevertheless, only few studies address their potential impacts and fate in aquatic organisms mainly because these compounds are considered non-toxic

Nano-zinc oxide (ZnO NPs), as widely used nanomaterials, are inevitably released into aquatic environments, posing potential threats to aquatic organisms. Mytilus galloprovincialis is a bivalve species sensitive to changes in marine ecological environments, but there has been limited research on its toxicity response to ZnO NPs. Therefore, we selected M. galloprovincialis as the research subject and

Yangtze finless porpoises (YFP) accumulate high levels of per- and polyfluoroalkyl substances (PFASs). However, the health impacts of PFASs to YFP are still unknown because it is technically and ethically unfeasible to use the critically endangered YFP in toxicological exposures. To uncover the potential toxicities of PFASs to YFP, this study exposed a YFP umbilical cord fibroblast cell line to perfluorobutane

There are many concerns about the impacts of Endocrine-Disrupting Chemicals on both wildlife and human populations. A plethora of chemicals have been shown to interfere with the Hypothalamic–Pituitary–Thyroid (HPT) axis in vertebrates. Disruption of the HPT axis is one of main endocrine criteria considered for the regulation of chemicals, along with the estrogen axis, androgen axis and steroidogenesis

This study evaluates the impact of environmentally relevant, low-concentration deltamethrin exposure to Eriocheir sinensis ovaries. Our findings revealed that even at a concentration of 0.05 µg/L, deltamethrin exposure can induce significant ovarian toxicity through a 5-day exposure, with gradual amplification detected with time, demonstrating the toxicity amplification effect. Hematoxylin and Eosin

New evidence regarding the risks that microplastics (MP) ingestion pose to human and wildlife health are being revealed with progress made in ecotoxicological research. However, comprehensive and realistic approaches that evaluate multiple physiological responses simultaneously are still scarce despite their relevance to understand whole-organism effects. To address this information gap, we performed

The study aims to investigate the effects of nano-alumina (AlNPs) on the early development and neurobehavior of zebrafish and the role of mTOR in this process. After embryos and grown-up larvae exposed to AlNPs from 0 to 200 μg/mL, we examined the development, neurobehavior, AlNPs content, and mTOR pathway genes. Moreover, embryos were randomly administered with control, negative control, mTOR knockdown

Global warming due to climate change, as well as freshwater eutrophication caused by anthropogenic activities are responsible, among other factors, for an increasing occurrence of harmful algal blooms (HABs) in aquatic systems. These can lead to the generation of cyanotoxins, secondary metabolites coming from cyanobacteria, producing adverse effects in living organisms including death. This research

The influence of inorganic mercury (Hg(II)) exposure on photosynthetic microorganisms and their pigments remains understudied. Here, we employed resonance Raman (RR) spectroscopy to investigate the responses of two freshwater phytoplankton species, the green alga Chlamydomonas reinhardtii and the diatom Cyclotella meneghiniana to Hg(II) exposure. We selectively recorded the spectral RR signature of

Bisphenol A analogues are largely used plasticisers that are going to replace bisphenol A in many sectors. Due to this replacement, their discharge and presence in the marine coastal areas are increasing, with unknown consequences for organisms and the trophic chain. This study assessed the effects of three different bisphenols (BPAF, BPF and BPS) - alone or as a mixture - provided via food (exposed

Tris (2-chloroethyl) phosphate (TCEP), a commonly used organophosphate flame retardant, has garnered considerable concern owing to its pervasive presence in the environment and its toxic effects on living organisms. The perpetuation of populations and species hinges on successful reproduction, yet research into the mechanisms underlying reproductive toxicity remains scant, particularly in aquatic species

Non-lethal biomonitoring should provide an innovative approach to establish bioethical protocols for the management of both aquaculture and wild fisheries resources. We aimed to assess non-lethal biomarkers in Piaractus mesopotamicus caged in a rice field during a bifenthrin (BF) application. We analyzed parameters related to the immune system, energy metabolism and oxidative stress in fish skin mucus