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Integration of physiology, microbiota and metabolomics reveals toxic response of zebrafish gut to co-exposure to polystyrene nanoplastics and arsenic
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2024-11-19 , DOI: 10.1016/j.aquatox.2024.107172 Guoxin Li, Min Lv, Haitao Yu, Heng zhang, Dandan Zhang, Qingsong Li, Lei Wang, Yicheng Wu
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2024-11-19 , DOI: 10.1016/j.aquatox.2024.107172 Guoxin Li, Min Lv, Haitao Yu, Heng zhang, Dandan Zhang, Qingsong Li, Lei Wang, Yicheng Wu
Both nanoplastic (NP) particles and arsenic (As) are widespread in aquatic environments and pose a combined risk of exposure to aquatic organisms. How the gut of aquatic organisms responds to combined risk of exposure is still unclear. In this study, zebrafish (Danio rerio ) were subjected to three distinct As stress environments: only As group (10 μg/L), and As combined with different concentrations of polystyrene (PS) NPs (1 mg/L and 10 mg/L) groups for 21 days via semi-static waterborne exposure. The physiological responses to combined stress, the diversity of gut microorganisms, and the metabolomic response of the gut were investigated. The findings indicated that PSNPs were prevalent in the intestines of zebrafish in the co-exposed group. Furthermore, the administration of 1 mg/L and 10 mg/L of PSNPs in the co-exposed group was observed to elevate As levels in the intestines by 24.88% and 76.95%, respectively, in comparison to As treatment alone. Simultaneous exposure of the gut to PSNPs and As resulted in increased contents/activities of MDA, SOD, CAT, and GST, and a decrease in contents/activities of GSH and GPx, when compared to As exposure alone. Additionally, the combined exposure led to an elevated expression of the Cu/Zn-sod, Mn-sod, gpx , and cat genes. The combined treatment with NPs and As resulted in an increase in the abundance of Proteobacteria and Fusobacteriota at the phylum level, as well as a significant increase in the abundance of Cetobacterium, Rhodococcus , and Bacteroides at the genus level. Non-targeted metabolomics analyses suggest that metabolic pathways affected by co-exposure include glycerophospholipid metabolism, glycerolipid metabolism, ABC transporters and autophagy. The findings of this study are of considerable significance for the evaluation of the toxicological impact of co-existing pollutants.
中文翻译:
生理学、微生物群和代谢组学的整合揭示了斑马鱼肠道对共暴露于聚苯乙烯纳米塑料和砷的毒性反应
纳米塑料 (NP) 颗粒和砷 (As) 都广泛存在于水生环境中,并且对接触水生生物构成综合风险。目前尚不清楚水生生物的肠道如何应对暴露的综合风险。在本研究中,斑马鱼 (Danio rerio) 经受了三种不同的 As 胁迫环境:仅 As 组 (10 μg/L) 和 As 与不同浓度的聚苯乙烯 (PS) NPs (1 mg/L 和 10 mg/L) 组合组通过半静态水暴露 21 天。研究了对综合应激的生理反应、肠道微生物的多样性和肠道的代谢组学反应。研究结果表明,PSNPs 在共暴露组斑马鱼的肠道中普遍存在。此外,与单独 As 治疗相比,观察到在共暴露组中施用 1 mg/L 和 10 mg/L PSNPs 使肠道中的 As 水平分别升高 24.88% 和 76.95%。与单独暴露于 As 相比,肠道同时暴露于 PSNPs 和 As 导致 MDA、SOD、CAT 和 GST 的含量/活性增加,而 GSH 和 GPx 的含量/活性降低。此外,联合暴露导致 Cu/Zn-sod、Mn-sod、gpx 和 cat 基因的表达升高。NPs 和 As 的联合处理导致门水平变形菌门和梭杆菌门的丰度增加,以及属水平上盲肠杆菌属、红球菌属和拟杆菌属的丰度显着增加。非靶向代谢组学分析表明,受共暴露影响的代谢途径包括甘油磷脂代谢、甘油脂代谢、ABC 转运蛋白和自噬。 本研究的结果对于评估共存污染物的毒理学影响具有重要意义。
更新日期:2024-11-19
中文翻译:
生理学、微生物群和代谢组学的整合揭示了斑马鱼肠道对共暴露于聚苯乙烯纳米塑料和砷的毒性反应
纳米塑料 (NP) 颗粒和砷 (As) 都广泛存在于水生环境中,并且对接触水生生物构成综合风险。目前尚不清楚水生生物的肠道如何应对暴露的综合风险。在本研究中,斑马鱼 (Danio rerio) 经受了三种不同的 As 胁迫环境:仅 As 组 (10 μg/L) 和 As 与不同浓度的聚苯乙烯 (PS) NPs (1 mg/L 和 10 mg/L) 组合组通过半静态水暴露 21 天。研究了对综合应激的生理反应、肠道微生物的多样性和肠道的代谢组学反应。研究结果表明,PSNPs 在共暴露组斑马鱼的肠道中普遍存在。此外,与单独 As 治疗相比,观察到在共暴露组中施用 1 mg/L 和 10 mg/L PSNPs 使肠道中的 As 水平分别升高 24.88% 和 76.95%。与单独暴露于 As 相比,肠道同时暴露于 PSNPs 和 As 导致 MDA、SOD、CAT 和 GST 的含量/活性增加,而 GSH 和 GPx 的含量/活性降低。此外,联合暴露导致 Cu/Zn-sod、Mn-sod、gpx 和 cat 基因的表达升高。NPs 和 As 的联合处理导致门水平变形菌门和梭杆菌门的丰度增加,以及属水平上盲肠杆菌属、红球菌属和拟杆菌属的丰度显着增加。非靶向代谢组学分析表明,受共暴露影响的代谢途径包括甘油磷脂代谢、甘油脂代谢、ABC 转运蛋白和自噬。 本研究的结果对于评估共存污染物的毒理学影响具有重要意义。