Azoxystrobin, a widely used fungicide, can contaminate water bodies through surface run-off, posing a risk to aquatic organisms. This study aimed to assess the genotoxic effects of azoxystrobin on the fish Pethia conchonius. The 96 h lethal concentration (LC50) of azoxystrobin was determined to be 0.514 mg/L. Based on LC50, three sub-lethal concentrations (SLCs) of 0.025 mg/L, 0.0514 mg/L, and 0.103 mg/L were used to expose fish for 96 h. The blood and gill samples were collected at 24 h intervals for analysis. The Micronucleus (MN) and Comet assays were used to evaluate nuclear abnormalities and DNA damage, respectively. The results showed that the frequency of nuclear abnormalities and DNA damage in the exposed groups was significantly higher than the control, with increasing concentrations and duration of exposure. The highest levels of micronuclei, notched and blebbed nuclei, and DNA damage parameters were observed in the group exposed to SLC III for 96 h. These findings indicate that azoxystrobin is highly genotoxic to fish causing severe DNA damage.

中文翻译：

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Azoxystrobin 在印度提斯塔河的淡水鱼 Pethia conchonius 中诱导遗传毒性


嘧菌酯是一种广泛使用的杀菌剂，可通过地表径流污染水体，对水生生物构成风险。本研究旨在评估嘧菌酯对 Pethia conchonius 鱼的遗传毒性作用。嘧菌酯的 96 h 致死浓度 （LC50） 测定为 0.514 mg/L。基于 LC50，使用 0.025 mg/L 、 0.0514 mg/L 和 0.103 mg/L 三种亚致死浓度 （SLC） 暴露鱼类 96 h。每隔 24 小时收集血液和鳃样本进行分析。微核 （MN） 和 Comet 检测分别用于评估核异常和 DNA 损伤。结果显示，暴露组核异常和 DNA 损伤的频率显著高于对照组，暴露浓度和持续时间增加。在 SLC III 暴露 96 h 的组中观察到最高水平的微核、缺口和起泡的细胞核以及 DNA 损伤参数。这些发现表明，嘧菌酯对鱼类具有高度遗传毒性，会导致严重的 DNA 损伤。