Cortisone is a naturally occurring corticosteroid hormone known for its wide range of anti-inflammatory and immunosuppressive effects, and it is commonly found in various aquatic environments. Previous reports have shown that cortisone can have significant negative impacts on fish; however, its specific effects on fish reproduction have not been thoroughly investigated. In this study, female adult zebrafish were exposed to 0.0 (control), 3.9, 40.2, and 377.9 ng/L of cortisone for 60 days, and multiple endpoints were evaluated. The results showed that as the concentration of cortisone increased, there was an increase in the percentage of perinuclear oocytes and a decrease in the proportion of late-stage oocytes, indicating a stagnation in oocyte development. Additionally, female zebrafish exposed to cortisone exhibited decreased attraction to males and reduced mating intimacy. Furthermore, exposure to cortisone resulted in changes in the development and behavior of zebrafish embryos. At cortisone concentrations of 3.9 and 40.2 ng/L, fewer eggs were laid and the survival rate of fertilized eggs decreased. These observed effects are associated with abnormal transcription levels of genes (Star, Cyp11a1, Cyp17, Cyp19a, Cyp11b, Hsd11β2, Hsd17β3) related to the HPG axis. These findings provided new insights into understanding potential environmental risks associated with corticosteroids.

中文翻译：

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可的松对雌性斑马鱼 （Dania rerio） 的影响：降低繁殖能力和后代存活率


可的松是一种天然存在的皮质类固醇激素，以其广泛的抗炎和免疫抑制作用而闻名，常见于各种水生环境中。以前的报告表明，可的松会对鱼类产生重大的负面影响;然而，它对鱼类繁殖的具体影响尚未得到彻底研究。在这项研究中，雌性成年斑马鱼暴露于 0.0 （对照）、3.9 、 40.2 和 377.9 ng/L 的可的松 60 天，并评估了多个终点。结果显示，随着可的松浓度的增加，核周卵母细胞的百分比增加，晚期卵母细胞的比例降低，表明卵母细胞发育停滞。此外，暴露于可的松的雌性斑马鱼对雄性的吸引力降低，交配亲密度降低。此外，暴露于可的松导致斑马鱼胚胎的发育和行为发生变化。在可的松浓度为 3.9 和 40.2 ng/L 时，产卵较少，受精卵的存活率降低。这些观察到的效果与与 HPG 轴相关的基因 （Star、Cyp11a1、Cyp17、Cyp19a、Cyp11b、Hsd11β2、Hsd17β3） 的异常转录水平有关。这些发现为理解与皮质类固醇相关的潜在环境风险提供了新的见解。