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个人简介

2018/06-至今 湖北大学生命科学院,教授 2014/01-2018/5 中国科学院天津工业生物技术研究所,访问学者/高级台湾青年访问学者 2012/01-2013/12 中国科学院微生物研究所,副研究员/高级台湾青年访问学者 2008/02-2011/12 台湾中研院特聘博士后 2003/09-2008/01 台湾大学,微生物学研究所,博士 1999/09-2001/07 台湾大学,医事技术学研究所,硕士 1995/09-1999/07 台湾大学,医事技术学系,学士

研究领域

结构生物学、酶应用与改造、酶的机理研究 酶是所有生命活动的发动机,酶的结构与催化机理研究对于了解生物基本运作、疾病发生原因、药物开发、以及医药产业与绿色生物工艺发展具有重大意义。陈纯琪教授通过结构生物学方法(X光晶体学与冷冻电镜)解析酶的三维结构与底物结合的复合体结构,阐释催化机理,并开展基于结构的理性设计,改造酶的特征与活性。工作内容主要涵盖三个体系:(1) 新型萜类合成酶;(2) 天然与人造合成高分子降解酶;(3) 霉菌毒素分解酶。

近期论文

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Chen, C. C.,* Zhang, L., Yu, X., Ma, L., Ko, T. P., Guo, R. T.* Versatile cis-isoprenyl Diphosphate Synthase Superfamily Members in Catalyzing Carbon–Carbon Bond Formation. ACS Catalysis, 2020, 10, 4717-4725 (Review, ACS Editors’ Choice, Cover story) Chen, C. C., Dai, L., Ma, L.,* Guo, R. T.* Enzymatic degradation of plant biomass and synthetic polymers Nature Reviews Chemistry, 2020, 4, 114-126 (Review, Cover story) Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C. C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E., Guo, R. T., Zhang, Y. A Structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity, 2019, 50, 1043-1053 Sun, H., Ko, T. P., Liu, W., Liu, W., Zheng, Y., Chen, C. C.,* Guo, R. T.* Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications, 2019, 521, 31-36. Kuo, C. J., Gao, J., Huang, J. W., Ko, T. P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y. F., Chen, T. H., Hu, Y., Yu, X., Guo, R. T.,* Chen, C. C.* Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. (2019) Biochimica et Biophysica Acta. General subjects, 2019, 1863, 1351-1359 Tang, X.# Xue, J.#, Yang, Y., Ko, T. P., Chen, C.Y., Dai, L., Guo, R.T. , Zhang, Y.,* Chen C. C.* Structural insights into the calcium dependence of Stig cyclases. RSC Advances, 2019, 9, 13182. Ma, J., Ko, T. P., Yu, X.,Zhang, L., Ma, L., Zhai, C., Guo, R. T., Liu, W., Li, H., Chen, C. C.* Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications, 2019, 515, 621-626. Zhang, L., Ko, T. P., Malwal S. R., Liu W., Zhou S., Oldfield E., Guo R. T.,* and Chen, C. C.* Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism, Biochemical and Biophysical Research Communications, 2019, 511, 800-805 Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T. P., Zhang, G., Liu, W., Huang, J. W., Dai, L., Guo, R. T., Major, D. T.,* Chen, C. C.* Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis, Organic Biomolecular Chemistry, 2019, 17, 2070-2076. (Cover story) Chen, C. C.,# Hu, X.,# Tang, X.,# Yang, Y.,# Ko, T. P., Gao, J., Zheng, Y., Huang, J. W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R. T.* The Crystal Structure of a Class of Cyclases that Catalyze the Cope Rearrangement. Angewandte Chemie International Edition, 2018, 57, 15060-15064. (Cover story) Chen, C. C.,# Han, X.,# Ko, T. P., Liu, W.,* Guo, R. T.* Structural studies reveal the molecular mechanism of PETase. FEBS Journal, 2018, 285, 3717-3723. Zheng, Y.,# Liu, W.,# Chen C. C.,# Hu, X., Liu, W., Ko, T. P., Tang, X., Wei, H., Huang, J. W., Guo, R. T.* Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity. ACS Catalysis. 2018, 8, 4294-4298. Han, X.,# Liu, W.,# Huang, J. W.,# Ma, J., Zheng, Y., Ko, T. P., Xu, L., Cheng, Y. S., Chen, C. C., Guo, R. T.* Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 2017, 13, 2106. Zhang, L.,# Chen, C. C.,# Ko, T. P.,# Huang, J. W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, Ke., Huang, C. C., Hsu, S. T., Wang, A. H., Oldfield, E.,* and Guo, R. T.* Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyl Transferase MoeN5, Angewandte Chemie International Edition, 2016, 55, 4716-4720. Xu, Z.,# Liu, W.,# Chen, C. C.,# Li Q., Huang, J. W., Ko, T. P., Liu, G., Liu, W., Peng, W., Cheng, Y. S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,* Guo, R. T.* Enhanced α‑Zearalenol Hydrolyzing Activity of a Mycoestrogen Detoxifying Lactonase by Structure-Based Engineering. ACS Catalysis, 2016, 6, 7657-7663. (Cover story) Liu, M.,# Chen, C. C.,# Chen, Lu.,# Xiao, X., Zheng, Y.,Huang, J. W., Liu, W., Ko, T. P., Cheng, Y. S., Feng, X., Oldfield, E.,* Guo, R. T.,* and Ma, Y.* Structure and Function of a “Head-to-Middle” Prenyltransferase: Lavandulyl Diphosphate Synthase, Angewandte Chemie International Edition, 2016, 55, 4721-4724. (Cover story) Huang, J. W.,# Liu, W.,# Lai, H. L., Cheng, Y. S., Zheng, Y., Li, Q., Sun, H., Kuo, C. J., Guo, R. T.,* Chen, C. C.* Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and Biophysical Research Communications, 2016, 16, 565-572. Yang, Y.,# Ko, T. P.,# Chen, C. C.,# Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S., O'Dowd, B., Huff, H., Huang, C. H., Docampo, R., Oldfield, E.,* Guo, R. T.,* Structures of Trypanosome Vacuolar Soluble Pyrophosphatases: Anti-Parasitic Drug Targets. ACS Chemical Biology, 2016, 11, 1362-1371. Haywood, J., Qi, J., Chen, C. C., Lu, G., Liu, Y., Yan, J., Shi, Y., and Gao, G. F. Structural basis of collagen recognition by human osteoclast-associated receptor and design of osteoclastogenesis inhibitors, PNAS, 2016, 113, 1038-1043. Cheng, Y. S.,# Chen, C. C.,# Huang, J. W., Ko, T. P., Huang, Z., and Guo, R. T.* Improving the catalytic performance of a GH11 xylanase by rational protein engineering, Applied Microbiology and Biotechnology, 2015, 99, 9503-9510.

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