儿茶素和咖啡因通过将分化的 3T3-L1 脂肪细胞从白色脂肪细胞转化为米色脂肪细胞来促进脂质代谢和产热,Journal of Food Science

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儿茶素和咖啡因通过将分化的 3T3-L1 脂肪细胞从白色脂肪细胞转化为米色脂肪细胞来促进脂质代谢和产热
Journal of Food Science ( IF 3.2 ) Pub Date : 2019-11-28 , DOI: 10.1111/1750-3841.14811
Chikako Sugiura _{1,

2} , Guodong Zheng ₃ , Litong Liu ₂ , Kazutoshi Sayama ₂

Affiliation

为了阐明儿茶素和咖啡因对脂肪细胞脂质代谢的影响并确定作用机制，将分化的 3T3-L1 脂肪细胞在含有 1、2.5、5 和 10 µg/mL 儿茶素以及 50 和 100 µg 咖啡因的培养基中培养/mL，单独或组合使用 8 天。儿茶素和咖啡因联合治疗强烈抑制细胞内脂质积累和甘油-3-磷酸脱氢酶活性。联合组PPARɤ、GLUT4、HSL、UCP-1和TMEM26的mRNA表达显着增加。这些发现表明联合治疗抑制了脂肪细胞中的脂质合成并改善了脂质代谢。此外，表明分化的3T3-L1脂肪细胞可以通过儿茶素和咖啡因从白色脂肪细胞转化为米色样脂肪细胞，因此，这种转化可以促进生热作用。实际应用：在这项研究中，我们揭示了儿茶素和咖啡因的联合治疗抑制了脂肪细胞的脂质合成并改善了脂质代谢。此外，该处理可能有助于从白色脂肪细胞转变为米色样脂肪细胞，这可以强烈促进产热量。

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Catechins and Caffeine Promote Lipid Metabolism and Heat Production Through the Transformation of Differentiated 3T3‐L1 Adipocytes from White to Beige Adipocytes

To elucidate effects of catechins and caffeine on lipid metabolism in adipocytes and identify the mechanism of action, differentiated 3T3-L1 adipocytes were incubated in culture media containing catechins at 1, 2.5, 5, and 10 µg/mL and caffeine at 50 and 100 µg/mL, singly or in combination, for 8 days. Intracellular lipid accumulation and glycerol-3-phosphate dehydrogenase activity were strongly suppressed by catechins and caffeine combination treatment. The mRNA expression of PPARɤ, GLUT4, HSL, UCP-1, and TMEM26 were significantly increased in the combined groups. These findings suggest that the combined treatment inhibited lipid synthesis and improved lipid metabolism in adipocytes. Moreover, it was indicated that the differentiated 3T3-L1 adipocytes could be transformed from white adipocytes to beige-like adipocytes by catechins and caffeine, and accordingly that this transformation could promote calorigenic action. PRACTICAL APPLICATION: In this study, we revealed that the combined treatment of catechins and caffeine inhibited lipid synthesis and improved lipid metabolism in adipocytes. Moreover, the treatment may contribute to the transforming from white adipocytes to beige-like adipocytes, which could strongly promote calorigenic action.

更新日期：2019-11-28

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