This experiment was conducted to determine the effect of an adsorbent material based on powdered alfalfa leaves added in the aflatoxin B₁ (AFB₁)-contaminated diet of turkey poults on production parameters, blood cell count, serum biochemistry, liver enzymes, and liver histology. For this purpose, three hundred and fifty female Nicholas-700 poults were randomly assigned into five treatments: (1) Control, AFB₁-free diet; (2) AF, diet contaminated with 250 ng AFB₁/g; (3) Alfalfa, AFB₁-free diet + 0.5% (w/w) adsorbent; (4) AF+alfalfa, diet contaminated with 250 ng AFB₁/g + 0.5% (w/w) adsorbent, and (5) AF+ yeast cell wall (YCW), diet contaminated with 250 ng AFB₁/g + 0.5% (w/w) of yeast cell wall (a commercial mycotoxin binder used as reference material). The in vivo efficacy of powdered alfalfa leaves was assessed during a 28-day period. In general, the addition of powdered alfalfa leaves in the AFB₁-free diet gave the best performance results (body weight, body weight gain, and feed intake) and improved the values of total protein, glucose, calcium, creatinine, and blood urea nitrogen. Moreover, the addition of powdered alfalfa leaves in the AFB₁-contaminated diet enhanced body weight and body weight gain and significantly reduced the feed intake, compared to the AF and AF+YCW groups. Additionally, significant alterations in serum parameters were observed in poults intoxicated with the AFB₁, compared to the Control group. Furthermore, typical histopathological lesions were observed in the liver of the AF group, which were significantly ameliorated with the addition of powdered alfalfa leaves. Conclusively, these results pointed out that low inclusion of powdered alfalfa leaves in the contaminated feed counteracted the adverse effects of AFB₁ in turkey poults.

本实验旨在确定在受黄曲霉毒素 B ₁ (AFB ₁ ) 污染的火鸡幼苗饲料中添加基于粉状苜蓿叶的吸附材料对生产参数、血细胞计数、血清生化、肝酶和肝脏组织学的影响。为此，将 350 只雌性 Nicholas-700 家禽随机分配到五种处理中：(1) 对照，不含 AFB ₁饮食； (2)AF，被250ng AFB ₁ /g污染的饮食； (3)苜蓿、无AFB ₁饲料+0.5%(w/w)吸附剂； (4) AF+苜蓿，被 250 ng AFB ₁ /g + 0.5% (w/w) 吸附剂污染的饮食，以及 (5) AF+ 酵母细胞壁 (YCW)，被 250 ng AFB ₁ /g + 0.5% 污染的饮食(w/w) 酵母细胞壁（用作参考材料的商业霉菌毒素结合剂）。在 28 天的时间内评估了苜蓿叶粉的体内功效。一般来说，在不含 AFB ₁的日粮中添加粉状苜蓿叶可获得最佳的生产性能结果（体重、体重增加和采食量），并提高总蛋白、葡萄糖、钙、肌酐和血尿素的值氮。此外，与AF组和AF+YCW组相比，在AFB ₁污染的日粮中添加粉状苜蓿叶可增加体重和体重增加，并显着降低采食量。另外，与对照组相比，在用AFB ₁中毒的家禽中观察到血清参数的显着变化。此外，在AF组的肝脏中观察到典型的组织病理学病变，添加苜蓿叶粉后，该病变明显改善。 最后，这些结果指出，受污染饲料中苜蓿叶粉的含量较低可以抵消 AFB ₁对火鸡幼苗的不利影响。