This experiment was conducted to determine the effect of an adsorbent material based on powdered alfalfa leaves added in the aflatoxin B₁ (AFB₁)-contaminated diet of turkey poults on production parameters, blood cell count, serum biochemistry, liver enzymes, and liver histology. For this purpose, three hundred and fifty female Nicholas-700 poults were randomly assigned into five treatments: (1) Control, AFB₁-free diet; (2) AF, diet contaminated with 250 ng AFB₁/g; (3) Alfalfa, AFB₁-free diet + 0.5% (w/w) adsorbent; (4) AF+alfalfa, diet contaminated with 250 ng AFB₁/g + 0.5% (w/w) adsorbent, and (5) AF+ yeast cell wall (YCW), diet contaminated with 250 ng AFB₁/g + 0.5% (w/w) of yeast cell wall (a commercial mycotoxin binder used as reference material). The in vivo efficacy of powdered alfalfa leaves was assessed during a 28-day period. In general, the addition of powdered alfalfa leaves in the AFB₁-free diet gave the best performance results (body weight, body weight gain, and feed intake) and improved the values of total protein, glucose, calcium, creatinine, and blood urea nitrogen. Moreover, the addition of powdered alfalfa leaves in the AFB₁-contaminated diet enhanced body weight and body weight gain and significantly reduced the feed intake, compared to the AF and AF+YCW groups. Additionally, significant alterations in serum parameters were observed in poults intoxicated with the AFB₁, compared to the Control group. Furthermore, typical histopathological lesions were observed in the liver of the AF group, which were significantly ameliorated with the addition of powdered alfalfa leaves. Conclusively, these results pointed out that low inclusion of powdered alfalfa leaves in the contaminated feed counteracted the adverse effects of AFB₁ in turkey poults.

进行本实验是为了确定在火鸡家禽的黄曲霉毒素 B₁ （AFB₁） 污染的饮食中添加的基于苜蓿叶粉的吸附材料对生产参数、血细胞计数、血清生化、肝酶和肝组织学的影响。为此，将 350 只雌性 Nicholas-700 幼禽随机分配到五个处理中：（1） 对照，无 AFB₁ 饮食;（2） AF，被 250 ng AFB₁/g 污染的饮食;（3） 紫花苜蓿、AFB_1-free 饮食 + 0.5% （w/w） 吸附剂;（4） AF+紫花苜蓿，被 250 ng AFB₁/g + 0.5% （w/w） 吸附剂污染的饮食，以及 （5） AF+ 酵母细胞壁 （YCW），被 250 ng AFB₁/g + 0.5% （w/w） 酵母细胞壁（用作参考物质的商业霉菌毒素吸附剂）污染的饮食。在 28 天内评估了苜蓿叶粉的体内功效。一般来说，在无 AFB₁ 的日粮中添加苜蓿叶粉给出了最好的性能结果（体重、体重增加和采食量），并提高了总蛋白、葡萄糖、钙、肌酐和血尿素氮的值。此外，与 AF 和 AF+YCW 组相比，在 AFB₁ 污染的日粮中添加苜蓿叶粉增加了体重和体重增加，并显着降低了采食量。此外，与对照组相比，在被 AFB₁ 中毒的家禽中观察到血清参数的显着变化。此外，在 AF 组肝脏中观察到典型的组织病理学病变，添加苜蓿叶粉后显著改善。 总之，这些结果表明，受污染饲料中苜蓿叶粉的含量低抵消了火鸡幼犬中 AFB₁ 的不利影响。