This project explored the antibacterial mechanism of didecyldimethylammonium bromide (DDAB) toward Staphylococcus aureus and Pseudomonas aeruginosa, and its removal effect on biofilms. Furthermore, we explored the effect of treatment by DDAB combined with slightly acidic electrolyzed water (SAEW) on biofilms of S. aureus or P. aeruginosa. First, DDAB has bacteriostatic and biofilm removal effects. Second, The effect of DDAB combined with SAEW on biofilm is more obvious than that of the two alone. DDAB at a concentration of 16 MIC combined with SAEW (ACC 30 mg/L, ORP 875 mV, pH 6.30) completely cleared the biofilm. In addition, the results of Confocal laser scanning microscopy (CLSM), Field emission scanning electron microscopy (FESEM) and Attenuated total reflection–Fourier-transform infrared spectroscopy (ATR-FTIR) showed that DDAB combined with SAEW could disrupt the structure of biofilms, reduce polysaccharides, proteins and phospholipids in biofilms. This method has the potential to be used in food production chains to control S. aureus and P. aeruginosa and their biofilms, and it can be used in other industries.

本项目探讨了二癸基二甲基溴化铵（DDAB）对金黄色葡萄球菌和铜绿假单胞菌的抑菌机制及其对生物膜的去除效果。此外，我们探讨了 DDAB 与微酸性电解水 (SAEW) 联合处理对金黄色葡萄球菌或铜绿假单胞菌生物膜的影响。首先，DDAB具有抑菌和去除生物膜的作用。其次，DDAB联合SAEW对生物膜的影响比两者单独作用更明显。浓度为 16 MIC 的 DDAB 与 SAEW（ACC 30 mg/L，ORP 875 mV，pH 6.30）完全清除生物膜。此外，共聚焦激光扫描显微镜（CLSM）、场发射扫描电子显微镜（FESEM）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）的结果表明，DDAB与SAEW结合可以破坏生物膜的结构，减少生物膜中的多糖、蛋白质和磷脂。该方法具有在食品生产链中控制金黄色葡萄球菌和铜绿假单胞菌及其生物膜的潜力，也可用于其他行业。