Isothiocyanates had attracted attention for many years due to their antimicrobial properties. In this study, the antifungal efficiency of eighteen isothiocyanate compounds were evaluated against six plant pathogenic fungi and two molds in vitro. Among them, 2-(4-methoxyphenyl)ethyl isothiocyanate displayed the highest antifungal activity against Aspergillus niger (EC₅₀ = 4.19 μg/mL). The further studies of its mode of action showed that it may damage the cell morphology and membrane integrity in a dose dependent manner by morphological observation, propyl iodide staining and the release of cellular contents. On the other hand, the decrease of related enzyme activities in tricarboxylic acid cycle suggested that 2-(4-methoxyphenyl)ethyl isothiocyanate may effected energy metabolism. Besides, reactive oxygen species, the content of malondialdehyde, the total antioxidant capacity, the content of glutathione and the kinase of oxidative reactive were changed in fungi treated with 2-(4-methoxyphenyl)ethyl isothiocyanate, compared with those in untreated fungi, which all indicated that 2-(4-methoxyphenyl)ethyl isothiocyanate exhibited antifungal activity against A.niger by inducing oxidative stress.

多年来，异硫氰酸酯因其抗菌特性而备受关注。在这项研究中，在体外评估了 18 种异硫氰酸酯化合物对 6 种植物病原真菌和 2 种霉菌的抗真菌效果。其中，2-(4-甲氧基苯基)乙基异硫氰酸酯对黑曲霉的抗真菌活性最高（EC ₅₀ = 4.19 微克/毫升）。对其作用方式的进一步研究表明，通过形态观察、碘丙啶染色和细胞内容物的释放，它可能以剂量依赖性方式损害细胞形态和膜完整性。另一方面，三羧酸循环中相关酶活性的降低表明2-(4-甲氧基苯基)乙基异硫氰酸酯可能影响能量代谢。此外，与未经处理的真菌相比，2-(4-甲氧基苯基)乙基异硫氰酸酯处理的真菌的活性氧、丙二醛含量、总抗氧化能力、谷胱甘肽含量和氧化反应激酶均发生了变化。均表明 2-(4-甲氧基苯基)乙基异硫氰酸酯对黑曲霉具有抗真菌活性通过诱导氧化应激。