<br>菊粉型果聚糖生物合成和降解中脱落酸与生长素和乙烯的串扰,Plant Biology

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菊粉型果聚糖生物合成和降解中脱落酸与生长素和乙烯的串扰
Plant Biology ( IF 4.2 ) Pub Date : 2021-03-12 , DOI: 10.1111/plb.13252
F Mohammadi ₁ , M R Naghavi ₁ , S A Peighambari ₁ , N Khosravi Dehaghi _{2,

3} , I Khaldari ₁ , E Bravi ₄ , O Marconi ₄ , G Perretti ₄

Affiliation

不同激素对果聚糖积累以及调节生物合成和降解的基因的影响是已知的；然而，有关果聚糖含量和平均聚合度 (mDP) 的激素相互作用机制的信息有限。
制备菊苣细胞悬浮培养物，并用脱落酸（ABA）、生长素（AUX）、乙烯（ETH）、ABA + AUX或ABA + ETH处理，然后测定菊粉浓度、菊粉的mDP和FAZY基因的表达。
低浓度的AUX和ETH增加了果聚糖含量，而高浓度的AUX和ETH则降低了果聚糖含量。外源 ABA 增加了菊粉的 mDP，这与1-FEHII的低表达相一致。在激素相互作用中，ABA改变并调整了AUX和ETH的作用。 ABA 与低水平的 AUX 和 ETH 一起导致菊粉含量减少和 mDP 增加，这与FEHII的低表达相一致。 ABA 与高水平的 AUX 和 ETH 一起导致菊粉含量增加，同时 mDP 降低，这与生物合成 ( 1-FFT ) 和降解 ( 1-FEHII ) 基因的高表达相一致。
AUX 和 ETH 的影响几乎相同，尽管 ETH 的影响更严重。 ABA 与 AUX 和 ETH 结合具有调节作用。在生物合成和降解基因中， 1-FEHII的表达受这些激素的影响更大。

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Abscisic acid crosstalk with auxin and ethylene in biosynthesis and degradation of inulin-type fructans in chicory

The effect of different hormones on fructan accumulation and the genes regulating biosynthesis and degradation is known; however, information on hormonal interaction mechanisms for fructan content and mean degree of polymerization (mDP) is limited.
Cell suspension cultures of chicory were prepared and treated with abscisic acid (ABA), auxin (AUX), ethylene (ETH), ABA + AUX or ABA + ETH, then inulin concentration, mDP of inulin and expression of FAZY genes was determined.
A low concentration of AUX and ETH increased fructan content, while a high concentration of AUX and ETH decreased it. Exogenous ABA increased mDP of inulin and this coincided with the low expression of 1-FEHII. In hormone interactions, ABA changed and adjusted the effect of both AUX and ETH. ABA, together with a low level of AUX and ETH, resulted in a decrease in inulin content and increase in mDP, which coincided with low expression of FEHII. ABA together with a high level of AUX and ETH caused an increase in inulin content with a lower mDP, which coincided with high expression of biosynthesis (1-FFT) and degradation (1-FEHII) genes.
The effect of both AUX and ETH was almost the same, although the effect of ETH was more severe. ABA had a modulating role in combinations with AUX and ETH. Among biosynthesis and degradation genes, the expression of 1-FEHII was more affected by these hormones.

更新日期：2021-03-12

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