Mixed self-assembled monolayers (Mixed SAMs) consisting of 8-Ferrocenyl-1-octanethiol and 6-Mercapto-1-hexanol (FcOT:MCH) with probe PNA on gold electrodes were fabricated by using two-step after the optimization of immobilization temperature of FcOT:MCH SAMs. Using AC voltammetry, a novel nucleic acid detection platform, with mixed SAMs, was proposed. A negative formal potential shift was observed after complementary ssDNA hybridization while there was no significant difference after non-complementary ssDNA hybridization. Compatible results were obtained with the measurement of formal potential differences between mixed SAMs and target DNA (complementary and non-complementary DNA) in different target DNA concentrations. The formal potential difference between mixed SAMs immobilization and complementary ssDNA hybridization was measured in different ionic strength concentrations.

混合自组装单层优化了FcOT：MCH SAMs的固定温度后，通过两步法，在金电极上用探针PNA制备了由8-二茂铁基-1-辛硫醇和6-巯基-1-己醇（FcOT：MCH）组成的（混合SAMs） 。使用交流伏安法，提出了一种具有混合SAM的新型核酸检测平台。互补的ssDNA杂交后观察到负的形式电位移位，而非互补的ssDNA杂交后没有显着差异。通过测量不同目标DNA浓度下混合SAM与目标DNA（互补和非互补DNA）之间的形式势差获得了兼容的结果。在不同的离子强度浓度下，测量了混合SAMs固定和互补ssDNA杂交之间的形式势差。