Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1.,Journal of Agricultural and Food Chemistry

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Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-08-31 , DOI: 10.1021/acs.jafc.0c03735
Xia Zhang ₁ , Min Gan ₂ , Jingyun Li ₃ , Hui Li ₁ , Meicheng Su ₁ , Dongfei Tan ₁ , Shaolei Wang ₁ , Man Jia ₁ , Liguo Zhang ₃ , Gang Chen ₁

Affiliation

l-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the aryl hydrocarbon receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Trp to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity.

中文翻译：

IL4I1介导的色氨酸代谢的内源性吲哚丙酮酸途径。

升色氨酸（TRP）被广泛用于食品和饲料执法发挥生物学过程中起重要作用。Trp的各种代谢物都发挥其强大的功能。吲哚丙酮酸途径是肠道菌群中Trp代谢的主要途径之一，提供了大量的吲哚衍生物，它们可以通过激活芳烃受体（AHR）信号传导途径来调节肠道的稳态和粘膜免疫。在这项研究中，我们构建了一个IL4I1过表达的293T细胞系，并发现IL4I1可以催化Trp产生吲哚-3-乙酸（IAA）和吲哚-3-甲醛（IAld）。此外，IAA和IAdd都积累在树突状细胞（DC）中，并且可以刺激CYP1A1的表达。我们的结果证明了以IL4I1为关键酶的宿主细胞中吲哚丙酮酸途径的存在。IL4I1介导的Trp代谢暗示饮食对免疫的影响。

更新日期：2020-09-30

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