Background: Dexpanthenol is a widely used humectant in hair care products, especially anti-hairfall products. The hair care industry is highly regulated in East Asia and treats products containing the combination of dexpanthenol, zinc pyrithione, and nicotinamide (vitamin B₃) as quasi-drugs. Objective: Because dexpanthenol lacks a UV chromophore, existing methodologies for analysis in finished products include pretreatments and/or HPLC-UV analysis at low wavelengths at which poor signal-to-noise is observed. These time-consuming methods lack the robustness needed for routine use in quality laboratories. This has resulted in the need for a simple, fast, accurate, and robust UHPLC-MS method to quantify dexpanthenol in hair care products that could be easily adapted in quality laboratories. Methods: The MS detection was performed in positive ion mode with data acquired in single-ion recording for dexpanthenol (206.14 m/z), dexpanthenol-d6 (212.29 m/z), and Leucine Enkephalin acetate salt (556.28 m/z). Quantitation was performed using peak area ratio of dexpanthenol to the internal standard. Results: The resulting linear curve R² was 0.9998 with sample precision RSDs <2.5%. The accuracy recoveries were within 2% and the robustness results were within 3% of the nominal conditions. Conclusions: The resulting method for the quantitation of dexpanthenol is fast, accurate, and robust in the range of 170.24-1024.5 ng/mL in shampoo and conditioner, which is easily adaptable in quality laboratories. Highlights: This study determined optimal sample preparation and UHPLC-MS conditions to quantify dexpanthenol in finished hair care products.

中文翻译：

---

分析护发产品中泛醇的创新方法。

背景：右泛醇是在护发产品（尤其是抗脱发产品）中广泛使用的保湿剂。护发行业在东亚受到严格监管，并将含有右泛醇，巯氧吡啶锌和烟酰胺（维生素B₃）的组合的产品视为准药物。目的：由于右泛醇缺乏紫外线生色团，因此成品中现有的分析方法包括预处理和/或低波长下的HPLC-UV分析，在该波长下观察到较差的信噪比。这些耗时的方法缺乏质量实验室常规使用所需的鲁棒性。因此，需要一种简单，快速，准确且耐用的UHPLC-MS方法来定量护发产品中的右旋泛醇，而该方法很容易在质量实验室中进行调整。方法：MS检测是在正离子模式下进行的，其中以单离子记录的方式获得了dexpanthenol（206.14 m / z），dexpanthenol-d6（212.29 m / z）和亮氨酸脑啡肽乙酸盐（556.28 m / z）的数据。使用右泛醇与内标的峰面积比进行定量。结果：线性曲线R²为0.9998，样品精密度RSD <2.5％。精度回收率在标称条件的2％以内，鲁棒性结果在3％以内。结论：所制得的右旋泛醇的定量方法在洗发剂和护发素中的定量结果为170.24-1024.5 ng / mL，快速，准确且可靠，很容易在质量实验室中应用。重点：这项研究确定了最佳的样品前处理方法和UHPLC-MS条件，以定量成品护发产品中的地芬太尼。