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成果及论文

期刊论文

2024

1. Wenshuai Wu; Ying Mu; Microfluidic technologies for advanced antimicrobial susceptibility testing, Biomicrofluidics, 2024, 18(3), 031504.   https://doi.org/10.1063/5.0190112

2. Wenshuai Wu; Qianbin Zhao; Boran Zhang; Viable Escherichia coli enumeration on a polydimethylsiloxane (PDMS) chip with vertical channel-well configuration, Microchim. Acta, 2024,191 (5), 241. https://www.researchgate.net/publication/379570069

3. Wenshuai Wu; Simon Chun Kiat Goh; Gaozhe Cai; Shilun Feng; Boran Zhang; Digital metabolic activity assay enables fast assessment of 2D materials bactericidal efficiency, Analytica Chimica Acta, 2024, 1285: 342007. https://www.researchgate.net/publication/375511698 

4. Haoqiang Feng; Shitao Shen; Mingliang Jin; Mengjie Xiao; Mengjun Liu; Qilin Zhang; Hongwei Jiang, Zichuan Yi; Wenshuai Wu; Guofu Zhou; Lingling Shui; Massive Electro‐Microfluidic Particle Assembly Patterns in Droplet Array for Information Encoding, Small, 2024, 2405161.  https://www.researchgate.net/publication/383822045  

2023

1. Wenshuai Wu; Boran Zhang; Weihong Yin; Liping Xia; Yuanjie Suo; Gaozhe Cai; Yang Liu; Wei Jin; Qianbin Zhao; Ying Mu; Enzymatic Antimicrobial Susceptibility Testing with Bacteria Identification in 30 min, Analytical Chemistry, 2023, 95(44): 16426-16432.   https://www.researchgate.net/publication/374954533      

2. Wenshuai Wu; Gaozhe Cai; Yang Liu; Yuanjie Suo; Boran Zhang; Wei Jin; Yinghua Yu; Ying Mu ; Direct single-cell antimicrobial susceptibility testing of Escherichia coli in urine using a ready-to-use 3D microwell array chip, Lab on a chip, 2023, 23(10), 2399-2410.   https://www.researchgate.net/publication/368521631     

3. Wenshuai Wu; Yuanjie Suo; Qianbin Zhao; Gaozhe Cai; Yang Liu; Wei Jin; Ying Mu; Boran Zhang; Inoculum size-insensitive susceptibility determination of urine sample based on in-situ measurement of inducible enzyme activity after 20 min of antibiotic exposure, Analytica Chimica Acta, 2023, 1282: 341858. https://www.researchgate.net/publication/374843455

4. Boran zhang; Wenhan Zhao; Dan Yuan; Tiao Wang; Wenshuai Wu*;A novel microparticle size sorting technology based on sheath flow stable expansion regimes, Physics of fluidics, 2023, 35: 052018. 通讯作者 ) https://doi.org/10.1063/5.0152581

5. Boran Zhang; Wenhan Zhao; Ziyu Wang; Qianbin Zhao; Wenshuai Wu*; Assistance of inertial microfluidics in membrane-based microfiltration, Journal of Water Process Engineering, 2023, 53: 103762 . 通讯作者 ) https://doi.org/10.1016/j.jwpe.2023.103762

6. Boran Zhang; Wenshuai Wu; Qianbin Zhao; Sheng Yan ; Geometric optimization of double layered microchannel with grooves array for enabling nanoparticle manipulation, Physics of Fluids, 2023, 35: 062009. https://doi.org/10.1063/5.0152929      

7. Wenhan Zhao; Xiaopeng Shang; Boran Zhang; Dan Yuan; Binh Thi Thanh Nguye; Wenshuai Wu; Jing Bo Zhang; Niancai Peng; Ai Qun Liu; Fei Duan; Lip Ket Chin ; Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection, Lab on a Chip, 2023, 23: 5039-5046. https://www.researchgate.net/publication/375217920      

8. Boran Zhang; Wenhan Zhao; Wenshuai Wu; Xiaopeng Shang; Binh Thi Thanh Nguyen; Jing Bo Zhang; Eric Peng Huat Yap; Ai Qun Liu; Lip Ket Chin ; Dean Vortex Trapping for Concentrating Escherichia coli in a Cross-Flow Microfilter, ACS Applied Engineering Materials, 2023, 1(10): 2809-2816. https://pubs.acs.org/doi/10.1021/acsaenm.3c00502    

2022

1. Wenshuai Wu; Binh Thi Thanh Nguyen; Patricia Yang Liu; Gaozhe Cai; Shilun Feng; Yuzhi Shi; Boran Zhang; Yuzhi Hong; Ruozhen Yu; Xiaohong Zhou; Ai Qun Liu; Yi Zhang; Eric Peng Huat Yap; Lip Ket Chin; Single Escherichia coli bacteria detection using a chemiluminescence digital microwell array chip., Biosensors and Bioelectronics, 2022, 215: 114594-114594.     https://www.researchgate.net/publication/362376489

2. Wenshuai Wu; Binh Thi Thanh Nguyen; Patricia Yang Liu; Gaozhe Cai; Shilun Feng; Yuzhi Shi; Boran Zhang; Yuzhi Hong; Ruozhen Yu; Xiaohong Zhou; Yi Zhang; Eric Peng Huat Yap; Ai Qun Liu; Lip Ket Chin; A self-driven carbon-doped high-density microwell array for single cell analysis, Sensors and Actuators B-chemical, 2022, 368: 132198-132198.  https://www.researchgate.net/publication/361384562   

3. Wenshuai Wu; Qianbin Zhao; Gaozhe Cai; Boran Zhang; Yuanjie Suo; Yang Liu; Wei Jin; Ying Mu; All-In-One Escherichia coli Viability Assay for Multi-dimensional Detection of Uncomplicated Urinary Tract Infections, Analytical Chemistry, 2022, 94(51): 17853-17860.   https://www.researchgate.net/publication/366361002  

4. Boran Zhang; Fan Yang; Wenshuai Wu*; Wuyi Wan; Wenhan Zhao; Qianbin Zhao; Investigation of particle manipulation mechanism and size sorting strategy in a double-layered microchannel., Lab on a Chip, 2022, 22(23): 4556-4573.    通讯作者 )   https://doi.org/10.1039/D2LC00822J

5. Boran Zhang; Siyuan Zou; Wenshuai Wu*; Fan Yang; Kashif Mehmood; Wuyi Wan; Qianbin Zhao ; Mechanism and Effects of Cellular Creep in a Microfluidic Filter, Journal of Physical Chemistry Letters, 2022, 13: 8641-8647.     通讯作者 )   https://pubs.acs.org/doi/10.1021/acs.jpclett.2c02137

6. Wenhan Zhao; Wenshuai Wu#; Dan Yuan; Siyuan Zou; Fan Yang; Qianbin Zhao; Kashif Mehmood; Boran Zhang; Experimental exploration on stable expansion phenomenon of sheath flow in viscous microfluidics, Physics of Fluids, 2022, 34(12): 122002.共同一作 )   https://doi.org/10.1063/5.0129764

7. Simon Chun Kiat Goh; Wenshuai Wu#; Chun Fei Siah; Derek Keng Yang Phee; Aiqun Liu; Beng Kang Tay ; Surface disinfection with silver loaded pencil graphite prepared with green UV photoreduction technique, Nanotechnology, 2022, 33(23): 235602.( 共同一作 )    https://www.researchgate.net/publication/358601726    

8. Yuanjie Suo; Weihong Yin; Wenshuai Wu; Wenjian Cao; Qiangyuan Zhu; Ying Mu ; A large-scale pico-droplet array for viable bacteria digital counting and dynamic tracking based on a thermosetting oil, Analyst, 2022, 147: 3305-3314.   https://doi.org/10.1039/D2AN00680D    

9. Yuanjie Suo; Weihong Yin; Qiangyuan Zhu; Wenshuai Wu; Wenjian Cao; Ying Mu ; A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection, Biosensors, 2022, 12(7): 458-458.  https://www.researchgate.net/publication/362314479

2021 

1. Wenshuai Wu; Shan Zhang; Tao Zhang; Ying Mu; Immobilized Droplet Arrays in Thermosetting Oil for Dynamic Proteolytic Assays of Single Cells, ACS Applied Materials & Interfaces, 2021, 13(5): 6081-6090. https://www.researchgate.net/publication/348839364      

2. Gaozhe Cai; Wenshuai Wu; Shilun Feng; Yuanjie Liu ; Label-free E. coli detection based on enzyme assay and a microfluidic slipchip, The Analyst, 2021, 146(14): 4622-4629. https://www.researchgate.net/publication/352204950      

3. Yuzhi Shi; Zhenyu Li; Patricia Yang Liu; Binh Thi Thanh Nguyen; Wenshuai Wu; Qianbin Zhao; Lip Ket Chin; Minggui Wei; Peng Huat Yap; Xiaohong Zhou; Hongwei Zhao; Dan Yu; Din Ping Tsai; Ai Qun Liu ; On-Chip Optical Detection of Viruses: A Review, Advanced Photonics Research, 2021, 2: 2000150. https://www.researchgate.net/publication/348515657 

2020    

1. Wenshuai Wu; Fengtian Wu; Shan Zhang; Xiong Ding; Tao Zhang; Ying Yang; Ying Mu; A self-powered bidirectional partition microfluidic chip with embedded microwells for highly sensitive detection of EGFR mutations in plasma of non-small cell lung cancer patients, Talanta, 2020, 220: 121426. https://www.researchgate.net/publication/343117588      

2. Yu He; Juxin Yin; Wenshuai Wu; Hongxiao Liang; Futianchun Zhu; Ying Mu; Hongliang Fan; Tao Zhang ; Rapid In Situ Photoimmobilization of a Planar Droplet Array for Digital PCR, Analytical Chemistry, 2020, 92(12): 8530-8535.   https://pubs.acs.org/doi/10.1021/acs.analchem.0c01304   

2019  

1. Tong Gou; Jiumei Hu; Shufang Zhou; Wenshuai Wu; Weibo Fang; Jingjing Sun; Zhenming Hu; Haotian Shen; Ying Mu ; A new method using machine learning for automated image analysis applied to chip-based digital assays, The Analyst, 2019, 144(10): 3274-3281.  https://doi.org/10.1039/C9AN00149B    

2. Zhenming Hu; Weibo Fang; Tong Gou; Wenshuai Wu; Jiumei Hu; Shufang Zhou; Ying Mu ; A novel method based on Mask R-CNN model for processing dPCR images, Analytical Methods, 2019, 11: 3410-3418.  https://doi.org/10.1039/C9AY01005J   

3. Jingjing Sun; Jiumei Hu; Tong Gou; Xiong Ding; Qi Song; Wenshuai Wu; Guoping Wang; Juxin Yin; Ying Mu ; Power-free polydimethylsiloxane femtoliter-sized arrays for bead-based digital immunoassays, Biosensors and Bioelectronics, 2019, 139: 111339. https://doi.org/10.1016/j.bios.2019.111339    

4. Jiumei Hu; Tong Gou; Wenshuai Wu; Jingjing Sun; Shan Zhang; Shufang Zhou; Juxin Yin; Ying Mu ; Proximity ligation assays for precise quantification of femtomolar proteins in single cells using self-priming microfluidic dPCR chip, Analytica Chimica Acta, 2019, 1076: 118-124.    https://doi.org/10.1016/j.aca.2019.05.034  

5. Shufang Zhou; Tong Gou; Jiumei Hu; Wenshuai Wu; Xiong Ding; Weibo Fang; Zhenming Hu; Ying Mu ; A highly integrated real-time digital PCR device for accurate DNA quantitative analysis, Biosensors and Bioelectronics, 2019, 128: 151-158.   https://doi.org/10.1016/j.bios.2018.12.055     

2018

1. Wu, Wenshuai; Zhou, Shufang; Hu, Jiumei; Wang, Guoping; Ding, Xiong; Gou, Tong; Sun, Jingjing; Zhang, Tao; Mu, Ying; A Thermosetting Oil for Droplet-Based Real-Time Monitoring of Digital PCR and Cell Culture, ADVANCED FUNCTIONAL MATERIALS, 2018, 39(28): 1803559. https://www.researchgate.net/publication/326871839    

2. Tong Gou; Jiumei Hu; Wenshuai Wu; Xiong Ding; Shufang Zhou; Weibo Fang; Ying Mu ; Smartphone-based mobile digital PCR device for DNA quantitative analysis with high accuracy, Biosensors and Bioelectronics, 2018, 120: 144-152 . https://doi.org/10.1016/j.bios.2018.08.030    

3. Pang, Bo; Fu, Kaiyue; Liu, Yushen; Ding, Xiong; Hu, Jiumei; Wu, Wenshuai; Xu, Kun; Song, Xiuling; Wang, Juan; Mu, Ying; Zhao, Chao; Li, Juan; Development of a self-priming PDMS/paper hybrid microfluidic chip using mixed-dye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection, ANALYTICA CHIMICA ACTA, 2018, 1040(1040): 81-89.  https://doi.org/10.1016/j.aca.2018.07.024    

2017

1. 吴文帅; 丁雄; 牟颖; 负压驱动皮升级等温核酸精确定量微流控芯片, 色谱, 2017, 35(3): 351-356.     

2. Pang, Bo; Ding, Xiong; Wang, Guoping; Zhao, Chao; Xu, Yanan; Fu, Kaiyue; Sun, Jingjing; Song, Xiuling; Wu, Wenshuai; Liu, Yushen; Song, Qi; Hu, Jiumei; Li, Juan; Mu, Ying ; Rapid and Quantitative Detection of Vibrio parahemolyticus by the Mixed-Dye-Based Loop-Mediated Isothermal Amplification Assay on a Self-Priming Compartmentalization Microfluidic Chip, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 51(65): 11312-11319. https://pubs.acs.org/doi/10.1021/acs.jafc.7b03655 

3. Wang, Guoping, Ding, Xiong, Hu, Jiumei, Wu, Wenshuai, Sun, Jing, Mu, Ying. Unusual isothermal multimerization and amplification by the strand-displacing DNA polymerases with reverse transcription activities. Scientific reports, 2017, 7(1), 1-10.   https://www.researchgate.net/publication/320593584

2015    

1. Ding, Xiong; Wu, Wenshuai; Zhu, Qiangyuan; Zhang, Tao; Jin, Wei; Mu, Ying; Mixed-Dye-Based Label-Free and Sensitive Dual Fluorescence for the Product Detection of Nucleic Acid Isothermal Multiple-Self-Matching-Initiated Amplification, Analytical Chemistry, 2015, 20(87): 10306-10314 .( 共同一作 ) https://www.researchgate.net/publication/282042474

会议论文

1. Zhang, J. B., Wu, W. S., Soh, W. M., Natesan, P. B., Yap, E. P. H., Zhang, B., ... & Liu, A. Q. (2023, May). Online Inspection of Microbial with an Opto-Microfluidic Chip. In CLEO: Applications and Technology (pp. ATh3I-4). Optica Publishing Group.

2. Cai, G., Feng, S., Liu, Y., Wu, W., Zhang, J., Lin, J., & Liu, A. Q. (2020, October). Design and microfabrication of microreactor array for plate reader for E. coli research. In 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 (pp. 434-435). Chemical and Biological Microsystems Society.

3. 吴文帅, 张珊, 朱强远, &牟颖. (2019). 微流控数字PCR 用于循环肿瘤DNA 高灵敏检测. 中国化学会第十二届全国微全分析系统学术会议、第七届全国微纳尺度生物分离分析学术会议、第七届国际微流控学学术论坛, ,1-1.

4. 张珊, 吴文帅, &牟颖. (2019). 基于静态液滴阵列的单个Jurkat细胞分泌的高通量分析. 中国化学会第十二届全国微全分析系统学术会议、第七届全国微纳尺度生物分离分析学术会议、第七届国际微流控学学术论坛, ,1-1.

申请专利

1. 牟颖, 吴文帅,金伟,一种可凝固的油相混合物及其应用, 中国发明专利,专利号:CN 108543504 B;(授权)

2. 牟颖, 吴文帅,金伟,一种通道内壁涂覆Parylene的微流控芯片制作方法, 中国发明专利,专利号:CN 108545692 B;(授权)

3. 牟颖, 吴文帅,一种快速的病原菌特异性酶促药敏分析方法, 中国发明专利,受理号:2023112474281;(审查)

4. 牟颖, 吴文帅,一种无接种效应的快速病原菌药敏分析方法, 中国发明专利,受理号:2023112476766;(审查)

5. 张博然, 吴文帅, 方越, 刘琳, 冉启华,一种基于PDMS微通道的被动式双体积液滴同步生成与收集芯片、装置及方法,中国发明专利,受理号:202410663209X;(审查)