Dayie, Kwaku - University of Maryland - Department of Chemistry & Biochemistry

个人简介

B.A., Physics with Honors, 1990, Hamilton College, Clinton, New York Ph.D., Biophysics, 1996, Harvard University, Cambridge MA (w. G. Wagner) Postdoctoral, 1998, Massachusetts Institute of Technology, Cambridge, MA (w. JR Williamson) Postdoctoral, 2000, The Scripps Research Institute, La Jolla, CA (w. JR Williamson)

研究领域

The structural and dynamic basis of RNA signaling in gene regulation of pathogenic bacteria; structure, interactions, dynamics, and function of RNA complexes involved in catalysis; chemical biological methods of labeling RNAs; RNA-Drug interactions, NMR methods development for large macromolecules

近期论文

查看导师最新文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Luo, Y.; Sintim, H.O.; Dayie, T.K. Synthesis of a Biotinylated Photocleavable Nucleotide Monophosphate for the Preparation of Natively Folded RNAs. Methods Enzymol. 2014, 549, 115-31.2.Alvarado, L.J.; Longhini, A.P.; LeBlanc, R.M.; Chen, B.; Kreutz, C.; Dayie, T.K. Chemo-enzymatic synthesis of selectively (13)C/(15)N-labeled RNA for NMR structural and dynamics studies. Methods Enzymol. 2014, 549, 133-62.3.Alvarado, L.J.; LeBlanc, R.M.; Longhini, A.P.; Keane, S.C.; Jain, N.; Yildiz, Z.F.; Tolbert, B.S.; D'Souza, V.M.; Summers, M.F.; Kreutz, C.; Dayie, T.K. Regio-selective chemical-enzymatic synthesis of pyrimidine nucleotides facilitates RNA structure and dynamics studies. Chembiochem. 2014, 15(11), 1573-7.4.Luo, Y.; Chen, B.; Zhou, J.; Sintim, H.O.; Dayie, T.K. E88, a new cyclic-di-GMP class I riboswitch aptamer from Clostridium tetani, has a similar fold to the prototypical class I riboswitch, Vc2, but differentially binds to c-di-GMP analogs. Mol. Biosyst. 2014, 10(3): 384-90.5.Berlin, K.; Longhini, A.; Dayie, T.K.; Fushman, D. Deriving quantitative dynamics information for proteins and RNAs using ROTDIF with a graphical user interface. J. Biomol. NMR 2013, 57(4), 333-52.6.Luo, Y.; Zhou, J.; Wang, J.; Dayie, T.K.; Sintim, H.O. Selective binding of 2'-F-c-di-GMP to Ct-E88 and Cb-E43, new class I riboswitches from Clostridium tetani and Clostridium botulinum respectively. Mol. Biosyst. 2013, 9(6), 1535-9.7.Nakayama, S.; Luo, Y.; Zhou, J.; Dayie, T.K.; Sintim, H.O. Nanomolar fluorescent detection of ci-di-GMP using a modular aptamer strategy. Chem. Comm. 2012, 48(72), 9059-61.8.Luo, Y.; Zhou, J.; Watt, S.K.; Lee, V.T.; Dayie, T.K.; Sintim, H.O. Differential binding of 2'-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins. Mol. Biosyst. 2012, 8(3), 772-8.9.Thakur, C.S.; Luo, Y.; Chen, B.; Eldho, N.V.; Dayie, T.K. Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E.coli mutant for labeling RNA for high resolution NMR, J. Biomol. NMR 2012, 52(2), 103-14.10.Thakur, C.S.; Dayie, T.K. Asymmetry of 13C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy, J. Biomol. NMR 2012, 52(1), 505-17. Chen, B.; Zuo, X.; Wang, Y.; Dayie, T.K., Multiple conformations of SAM-II riboswitch detected with SAXS and NMR spectroscopy. Nucleic Acids Res. 2011, 40(7), 3117-30.2.Dayie, T.K. Nucleic Acids: Dynamics Studies by Solution NMR. Encyclopedia of Nuclear Magnetic Resonance 2011. (D. M. Grant and R. K. Harris, eds.), DOI: 10.1002/9780470034590.emrstm1237.3.Alvarado, L.J.; Ash, D.; Dayie, T.K. Activity measurements of pentose phosphate pathway enzymes using the Thermo Scientific NanoDrop 2000c kinetics application. ThermoScientific Application Note 2011, T118-Revised 8/2011.4.Luo, Y.; Eldho, N. V.; Sintim, H. O.; Dayie, T. K. RNAs synthesized using photocleavable biotinylated nucleotides have dramatically improved catalytic efficiency. Nucleic Acids Res. 2011, 39(19), 8559-71.5.Arthur, P. K.; Alvarado, L. J.; Dayie, T. K. Expression, purification and analysis of the activity of enzymes from the pentose phosphate pathway. Protein Expr. Purif. 2011, 76(2), 229-37.6.Thakur, C.S.; Brown, M.E.; Sama, J.N.; Jackson, M.E.; Dayie, T.K. Growth of wildtype and mutant E. coli strains in minimal media for optimal production of nucleic acids for preparing labeled nucleotides. Appl. Microbiol. Biotechnol. 2010, 88(3), 771-9.7.Thakur, C.S.; Sama, J.N.; Jackson, M.E.; Chen, B.; Dayie, T.K. Selective (13)C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle. J Biomol. NMR 2010, 48(4), 179-92.8.Chen, Y.; Eldho, N.V.; Dayie, T.K.; Carey, P.R. Probing Adenine Rings and Backbone Linkages Using Base Specific Isotope-Edited Raman Spectroscopy: Application to Group II Intron Ribozyme Domain V. Biochemistry 2010, 49(16), 3427-35.9.Dayie, T.K.; Thakur, C.S. Site-specific labeling of nucleotides for making RNA for high resolution NMR studies using an E. coli strain disabled in the oxidative pentose phosphate pathway. J Biomol. NMR 2010, 47(1), 19-31.