当前位置: X-MOL首页全球导师 国内导师 › 李健

个人简介

2013年9月毕业于德国柏林工业大学(Technische Universität Berlin),获得自然科学博士学位(Dr. rer. nat.);随后于2013年12月至2017年8月在美国西北大学(Northwestern University)从事博士后研究;2017年9月加入上海科技大学物质科学与技术学院,任助理教授、研究员、博士生导师。2018年获上海市“浦江人才计划(A类)”资助。2018年8月起任Springer Nature出版社《SN Applied Sciences》杂志编委。2019年4月起担任中国生物工程学会青年工作委员会委员。2023年10月起担任上海市生物工程学会理事。2020年12月获得上海科技大学“优秀教师”荣誉称号。2022年3月起担任《Frontiers in Natural Products》杂志副主编 (Associate Editor)。

研究领域

1. 新型无细胞蛋白合成体系的开发和利用 2. 无细胞生物合成具有重要工业和药用价值的蛋白质 3. 天然产物药物(如非核糖体多肽类抗生素)的生物合成、发现与开发 4. 无细胞代谢工程及生物催化与生物转化 5. 新型蛋白质生物材料的设计、开发与应用

近期论文

查看导师新发文章 (温馨提示:请注意重名现象,建议点开原文通过作者单位确认)

Ouyang J, Zhang Z, Li J*, Wu C*. Integrating enzymes with supramolecular polymers for recyclable photobiocatalytic catalysis. Angewandte Chemie International Edition, 2024, e202400105 Ba F, Zhang Y, Ji X, Liu WQ, Ling S, Li J*. Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology. Biotechnology Journal, 2024, 19, 2300327 Lu H, Ouyang J, Liu WQ, Wu C*, Li J*. Enzyme-polymer-conjugate-based Pickering emulsions for cell-free expression and cascade biotransformation. Angewandte Chemie International Edition, 2023, e202312906 Ba F, Zhang Y, Ji X, Liu WQ, Ling S, Li J*. Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology. Biotechnology Journal, 2023, 2300327 Zhang Y, Liu WQ, Li J*. Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold. Synthetic and Systems Biotechnology, 2023, 8, 610–617 Wang Y, Gao W, Yang S, Chen Q, Ye C, Wang H, Zhang Q, Ren J, Ning Z, Chen X, Shao Z, Li J, Liu Y, Ling S. Humanoid intelligent display platform for audiovisual interaction and sound identification. Nano-Micro Letters, 2023, 15, 221 Shi Q, Li J, Liu C, Zhai X, Chen L, Zhang Y, Feng D, Zhang R, Li J, Ling S, Zheng L, Luo Y, Liu Y. Fluorescence-coded logarithmic-dilution digital droplet PCR for ultrawide-dynamic-range nucleic acid quantification. Biosensors and Bioelectronics, 2023, 241, 115702 Ba F, Zhang Y, Wang L, Liu WQ, Li J*. Applications of serine integrases in synthetic biology over the past decade. SynBio, 2023, 1, 172–189 Peng R#, Ba F#, Li J#, Cao J, Zhang R, Liu WQ, Ren J, Liu Y*, Li J*, Ling S*. Embedding living cells with a mechanically reinforced and functionally programmable hydrogel fiber platform. Advanced Materials, 2023, 35, 2305583 Dong Z, Peng R, Zhang Y, Shan Y, Ding W, Liu Y, Li J, Zhao M, Jiang LB, Ling S. Tendon repair and regeneration using bioinspired fibrillation engineering that mimicked the structure and mechanics of natural tissue. ACS Nano, 2023, 17, 17858–17872 Zhu J, Dai C, Yang H, Shan Y, Ren J, Liu Y, Li J, Chen W, Liang J, Ling S. IntelliSense bio-ionotronics battery for early warning of geological seepage. ACS Sensors, 2023, 8, 2731–2739 Zhang R, Liu WQ, Ling S, Li J*. Combining cell-free expression and multifactor optimization for enhanced biosynthesis of cinnamyl alcohol. Journal of Agricultural and Food Chemistry, 2023, 71, 8551–8557 Chen Y, Liu WQ, Zheng X, Liu Y, Ling S, Li J*. Cell-free biosynthesis of lysine-derived unnatural amino acids with chloro, alkene, and alkyne groups. ACS Synthetic Biology, 2023, 12, 1349–1357 (Highlighted as ACS Editors' Choice on May 4, 2023) Meng Y, Yang M, Liu WQ, Li J*. Cell-free expression of a therapeutic protein serratiopeptidase. Molecules, 2023, 28, 3132 Ba F, Ji X, Huang S, Zhang Y, Liu WQ, Liu Y, Ling S, Li J*. Engineering Escherichia coli to utilize erythritol as sole carbon source. Advanced Science, 2023, 10, 2207008 Zhang H, Cao L, Li J, Liu Y, Lv Z, Ren J, Shao Z, Ling S. Dual physically crosslinked silk fibroin ionoelastomer with ultrahigh stretchability and low hysteresis. Chemistry of Materials, 2023, 35, 1752–1761 Huang S, Ba F, Liu WQ, Li J*. Stapled NRPS enhances the production of valinomycin in Escherichia coli. Biotechnology and Bioengineering, 2023, 120, 793–802 Liu Y, Liu WQ, Huang S, Xu H, Lu H, Wu C, Li J*. Cell-free metabolic engineering enables selective biotransformation of fatty acids to value-added chemicals. Metabolic Engineering Communications, 2023, 16, e00217 Liu WQ, Ji X, Xu H, Lu Y, Li J*. Cell-free protein synthesis system enables rapid and efficient biosynthesis of restriction endonucleases. Synthetic Biology Journal (in Chinese), 2023, 4, 840–851 Li J*. Cellular decoding for non-natural peptides. Nature Chemistry, 2023, 15, 7–8 Moore SJ, Lai HE, Li J, Freemont PS. Streptomyces cell-free systems for natrual product discovery and engineering. Natural Product Reports, 2023, 40, 228–236 Li L, Zhang R, Chen L, Tian X, Li T, Pu B, Ma C, Ji X, Ba F, Xiong C, Shi Y, Mi X, Li J*, Keasling JD, Zhang J*, Liu Y*. Permeability-engineered compartmentation enables in vitro reconstitution of sustained synthetic biology systems. Advanced Science, 2022, 2203652 Yang C#, Yang M#, Zhao W, Ding Y, Wang Y*, Li J*. Establishing a Klebsiella pneumoniae-based cell-free protein synthesis system. Molecules, 2022, 27, 4684 Sun Z, Hübner R, Li J, Wu C. Artificially sporulated Escherichia coli cells as a robust cell factory for interfacial biocatalysis. Nature Communications, 2022, 13, 3142 Zhang Y, Chen W, Wu D, Liu Y, Wu Z, Li J, Zhang SY, Ji Q. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli. Nucleic Acids Research, 2022, 50, 5948–5960 Li J*. Engineered microbes: Making valuable chemicals from waste gases. Synthetic and Systems Biotechnology, 2022, 7, 837–838 Tian X, Liu WQ, Xu H, Ji X, Liu Y, Li J*. Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan. Synthetic and Systems Biotechnology, 2022, 7, 775–783 Liu Y, Ba F, Liu WQ, Wu C, Li J*. Plug-and-play functionalization of protein-polymer conjugates for tunable catalysis enabled by genetically encoded "click" chemistry. ACS Catalysis, 2022, 12, 4165–4174 Ji X#, Liu WQ#, Li J*. Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis. Current Opinion in Microbiology, 2022, 67, 102142 (Invited review) Ba F, Liu Y, Liu WQ, Tian X, Li J*. SYMBIOSIS: Synthetic manipulable biobricks via orthogonal serine integrase systems. Nucleic Acids Research, 2022, 50, 2973–2985 (Faculty Opinions Recommended as Exceptional) Xu H, Yang C, Tian X, Chen Y, Liu WQ, Li J*. Regulatory part engineering for high-yield protein synthesis in an all-Streptomyces-based cell-free expression system. ACS Synthetic Biology, 2022, 11, 570–578 Xu H, Liu WQ, Li J*. A Streptomyces-based cell-free protein synthesis system for high-level protein expression. Methods in Molecular Biology, 2022, 2433, 89–103 Li J*, Kwon YC*, Lu Y*, Moore SJ*. Editorial: Cell-free synthetic biology. Frontiers in Bioengineering and Biotechnology, 2021, 9, 799122 Yang C, Liu Y, Liu WQ, Wu C, Li J*. Designing modular cell-free systems for tunable biotransformation of L-phenylalanine to aromatic compounds. Frontiers in Bioengineering and Biotechnology, 2021, 9, 730663 Huang S#, Liu Y#, Liu WQ, Neubauer P*, Li J*. The nonribosomal peptide valinomycin: From discovery to bioactivity and biosynthesis. Microorganisms, 2021, 9, 780 (Highly Cited Paper) Feng J#, Yang C#, Zhao Z, Xu J, Li J*, Li P*. Application of cell-free protein synthesis system for the biosynthesis of L-theanine. ACS Synthetic Biology, 2021, 10, 620–631 Liu WQ, Wu C*, Jewett MC, Li J*. Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system. Biotechnology and Bioengineering, 2020, 117, 4001–4008 Zhang L, Liu WQ, Li J*. Establishing a eukaryotic Pichia pastoris cell-free protein synthesis system. Frontiers in Bioengineering and Biotechnology, 2020, 8, 536 Xu H, Liu WQ, Li J*. Translation related factors improve the productivity of a Streptomyces-based cell-free protein synthesis system. ACS Synthetic Biology, 2020, 9, 1221–1224 Zhuang L#, Huang S#, Liu WQ, Karim AS, Jewett MC*, Li J*. Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin. Metabolic Engineering, 2020, 60, 37–44

推荐链接
down
wechat
bug