罗晖 - 北京科技大学 - 化学与生物工程学院

个人简介

1、2009-2010麻省理工学院访问学者 2、2004年至今北京科技大学教师 3、2004年清华大学获生物化工博士学位 4、1999年北京化工大学获生物化工硕士学位 5、1996年北京化工大学获生物化工学士学位

研究领域

酶工程，医药中间体，疫苗，合成生物学

近期论文

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Tian J, Jia R, Dong W, Sun H, Wang Y, Chang Y, Luo H*. (2021). One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry. Biotechnology Letters, 43(5), 1075-1087. Sun H, Liu T, Luo H*, Nie Z, Chang Y, Yu H, Shen Z. Optimization of Cephalosporin C Acylase Expression in Escherichia coli by High-Throughput Screening a Constitutive Promoter Mutant library. Applied Biochemistry and Biotechnology, 2021, 193(4):1056-1071. Liu J, Tong S, Sun H, Chang Y, Luo H*, Yu H, Shen Z. Effect of shaking speed on immobilization of cephalosporin C acylase: Correlation between protein distribution and properties of the immobilized enzymes. Biotechnology Progress. 2020, 37(1):e3063 Wang Y, Chang Y, Jia R, Sun H, Tian J, Luo H*, Yu H, Shen Z. SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability. Process Biochemistry, 2020, 95:260-268 Nie Z, Luo H*, Li J, Sun H, Xiao Y, Liu T, Chang Y, Yu H, Shen Z. High-throughput screening of T7 promoter mutants for soluble expression of cephalosporin C acylase in E. coli. Applied Biochemistry and Biotechnology, 2020, 190:293-304 Chang Y, Tong S, Luo H*, Liu Z, Qin B, Zhu L, Sun H, Yu H, Shen Z. (2019). Application of ammonium bicarbonate buffer as a smart microenvironmental pH regulator of immobilized cephalosporin C acylase catalysis in different reactors. Biotechnology Progress. 2019, 35(5):e2846 Wang Y, Tian J, Xiao Y, Wang Y, Sun H, Chang Y, Luo H*. SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase. Biotechnology Letters, 2019, 41(8-9):987-994 Luo H*, Han K, Chang Y, Wang Y, Tong S, Nie Z, Wang X, Wei Y, Yu H, Shen Z. Effects of Inhibitors on the Catalysis and Immobilization of Cephalosporin C Acylase. Chem. Biochem. Eng. Q., 2018, 32(1):125-133 Luo H*, Zhu L, Chang Y, Liu X, Liu Z, Sun H, Li X, Yu H, Shen, Z. Microenvironmental pH changes in immobilized cephalosporin C acylase during a proton-producing reaction and regulation by a two-stage catalytic process. Bioresource Technology, 2017, 223:157-165 Xu J, Luo H*, López C, Xiao J, Chang Y*. Novel immobilization process of a thermophilic catalase: efficient purification by heat treatment and subsequent immobilization at high temperature. Bioprocess and Biosystems Engineering, Bioprocess Biosyst Eng, 2015, 38:1983-1991 Luo H*, Ma J, Chang Y, Yu H, Shen Z. Directed evolution and mutant characterization of nitrilase from Rhodococcus rhodochrous tg1-A6. Appl Biochem Biotechnol, 2016, 178(8):1510-1521 Luo H*, Zhao H, Chang Y, Wang Q, Yu H, Shen Z. Oriented immobilization and characterization of a poly-lysine tagged cephalosporin C acylase on glyoxyl agarose support. Appl Biochem Biotechnol, 2015, 175:2114-2123 He H, Wei Y, Luo H*, Chang Y, Yu H, Shen Z. Immobilization and stabilization of cephalosporin C acylase on aminated support by cross-linking with glutaraldehyde and post-modification using aminated macromolecules. Biotechnology Progress, 2015, 31(2):387-95 Wei Y, Luo H*, Chang Y, Yu H, Shen Z. Reversible immobilization of cephalosporin C acylase on epoxy supports coated with polyethyleneimine. Biocatalysis and Biotransformation, 2015, 33(5-6):250-259