个人简介

Work done by muscle and non-muscle cells requires the transduction of chemical into mechanical energy. This function is carried out by actin, tubulin, and a family of motor proteins. The interactions between these proteins and the changes in their structure constitute the molecular basis for force generation and motility in muscle and non-muscle cells. Our goal of elucidating the mechanism of contractile processes is pursued at two levels. At the molecular level, we are concerned with the structural and dynamic properties of the contractile proteins. We explore structure-function relationships in actin, myosin and other proteins by biochemical, biophysical, immunochemical, mutational, and in the vitro motility and force measurements. Intermediate steps in the contractile process are probed with the help of nucleotide analogues, specific antibodies, synthetic peptides, and appropriate mutants of the key proteins. The aim of these studies is to obtain a structural description of the mechanism of motion and force generation by motor proteins., At the cellular level, we study the function, interactions, and structural transitions of the assembled protein systems. Many filamentous cellular structures are constructed from their components through fine-tuned assembly processes. The biological function of these filaments is frequently linked to the signaling and the regulation of assembly by cellular factors. Our interest is focused on the formation and remodeling of actin filaments, bundles, and networks by actin binding proteins. The mechanisms of the assembly reactions and the structural changes which govern them are studied by biophysical, biochemical, and electron microscopy methods. To understand the regulation of actin assembly by other proteins, macromolecular contact sites are investigated by a wide spectrum of experimental techniques.

研究领域

Biochemistry/Biophysics/Structural and Computational Biology/Systems Biology and Biological Regulation/Proteomics and Bioinformatics

Dr. Reisler received his B.A. from the Hebrew University in Jerusalem, Israel and his Ph.D. from the Weizmann Institute of Science in Rehovot, Israel. He was a Postdoctoral Scholar in the Biology Department of the Johns Hopkins University.

近期论文

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drebrin Inhibits Cofilin-Induced Severing of F-Actin. Grintsevich Elena E, Reisler Emil Cytoskeleton (Hoboken). 2014 Jul 22. doi: 10.1002/cm.21184.[Epub ahead of print]. Nanostructured self-assembly of inverted formin 2 (INF2) and F-actin-INF2 complexes revealed by atomic force microscopy Sharma Shivani, Grintsevich Elena E, Woo JungReem, Gurel Pinar S, Higgs Henry N, Reisler Emil,Gimzewski James K Langmuir. 2014 Jul 1;30(25):7533-9. INF2-mediated severing through actin filament encirclement and disruption. Gurel Pinar S, Ge Peng,Grintsevich Elena E, Shu Rui, Blanchoin Laurent, Z. Hong Zhou,Reisler Emil, Higgs Henry N. Curr Biol. 2014 Jan 20;24(2):156-64. Cofilin-induced changes in F-actin detected via cross-linking with benzophenone-4-maleimide. Chen Christine K, Benchaar Sabrina A, Phan Mai, Grintsevich Elena E, Loo Rachel R, Loo Joseph A, Reisler Emil Biochemistry. 2013 Aug 13;52(32):5503-9. Correlative nanoscale imaging of actin filaments and their complexes. Sharma Shivani, Zhu Huanqi, Grintsevich Elena E, Reisler Emil, Gimzewski James K. Nanoscale. 2013 Jul 7;5(13):5692-702. Drebrin-induced stabilization of actin filaments Mikati Mouna A, Grintsevich Elena E, Reisler Emil J Biol Chem. 2013 Jul 5;288(27):19926-38 ATP and ADP actin states Dmitri S. Kudryashov, Reisler Emil Biopolymers. 2013 Apr;99(4):245-56. Identification of cation-binding sites on actin that drive polymerization and modulate bending stiffness Hyeran Kang, Michael J. Bradley, Brannon R. McCullough, Anaëlle Pierre, Elena E.Grintsevich, Emil Reisler, Enrique M. De La Cruza Proc Natl Acad Sci U S A. 2012 Oct 16;109(42) Structural states and dynamics of the D-loop in actin. Durer Zenep A, Kudryashov Dmitri S, Sawaya Michael R, Altenbach Christian, Hubbell Wayne, Reisler Emil Biophys J. 2012 Sep 5;103(5):930-9. Molecular cooperativity of drebrin1-300 binding and structural remodeling of F-actin. Sharma Shivani, Grintsevich Elena E, Hsueh Carlin, Reisler Emil, Gimzewski James K. Biophys J. 2012 Jul 18;103(2):275-83.