陈兴 - 北京大学 - 化学与分子工程学院

个人简介

2017 – 至今北大-清华生命科学联合中心高级研究员 2016 – 至今北京大学化学与分子工程学院教授 2011 – 至今北京大学合成与功能生物分子中心研究员 2011 – 2017 北大-清华生命科学联合中心研究员 2010 – 2016 北京大学化学与分子工程学院研究员 2007 – 2010 哈佛大学医学院博士后 2007 加州大学伯克利分校化学博士 2002 清华大学化学学士

研究领域

化学与生命科学的交叉融合为分析、理解和操控生物学过程开辟了一条全新的研究途径。我们课题组的研究兴趣集中于化学糖生物学和生物纳米技术。我们综合运用化学方法、生物手段和纳米技术，研究糖基化的生物学功能及其在代谢疾病及其心血管并发症中的作用主要研究内容包括活细胞及活体中聚糖的化学标记糖的合成、成像和组学分析糖基化在神经系统和干细胞中的功能糖代谢与糖基化的内在联系基于生物正交反应的化学生物学技术

近期论文

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Tang, Q.; Chen, X.* “ Nascent Proteomics: Chemical Tools for Monitoring Newly Synthesized Proteins” Angew. Chem. Int. Ed. e202305866 (2023). Sun, J.#; Huang, Z.#; Du, Y.#; Lv, P.; Fan, X.; Dai, P.*; Chen, X.* “ Metabolic Glycan Labeling in Primary Neurons Enabled by Unnatural Sugars with No S-Glyco-Modification” ACS Chem. Biol. 18, 1416-1424 (2023). Zhang, X.#; Tang, Q.#; Sun, J.; Guo, Y.; Zhang, S.; Liang, S.; Dai, P.; Chen, X.* “ Cellular-Scale Proximity Labeling for Recording Cell Spatial Organization in Mouse Tissues” Sci. Adv. 9, eadg6388 (2023). Hao, Y.#, Li, X.#, Qin, K., Shi, Y., He, Y., Zhang, C., Cheng, B., Zhang, X., Hu, G., Liang, S., Tang, Q., Chen, X.* “ Chemoproteomic and Transcriptomic Analysis Reveals that O-GlcNAc Regulates Mouse Embryonic Stem Cell Fate through the Pluripotency Network” Angew. Chem. Int. Ed. 62, e202300500 (2023). Li, X.#, Lei, C.#, Song, Q., Bai, L., Cheng, B., Qin, K., Li, X., Ma, B., Wang, B., Zhou, W., Chen, X.*, Li, J.* “ Chemoproteomic profiling of O-GlcNAcylated proteins and identification of O-GlcNAc transferases in rice” Plant Biotechnol. J. 21, 742-753 (2023). Cheng, B.*#, Wang, C.#, Hao, Y.#, Wang, J., Xia, X., Zhang, H., He, R., Zhang, S., Dai, P., Chen, X.* “ Facile Synthesis of Clickable Unnatural Sugars in the Unprotected and 1,6-Di-O-Acylated Forms for Metabolic Glycan Labeling” Chem. Eur. J. 29, e202203054 (2023). Liu, W.#; Tang, Q.#; Meng, L.#; Hu, S.; Sun, D.; Li, S.; Dai, P.*; Chen, X.* “ Interbacterial Chemical Communication-Triggered Nascent Proteomics” Angew. Chem. Int. Ed. 62, e202214010 (2023). Wu, J.; Lei, C.; Li, X.; Dong, X.; Qin, K.; Hong, W.; Li, J.; Zhu, Y.*; Chen, X.* “ Chemoproteomic Profiling of O-GlcNAcylation in Arabidopsis Thaliana by Using Metabolic Glycan Labeling” Isr. J. Chem. 298, e202200065 (2022). Shi, Y.; Yan, S.; Shao, G.; Wang, J.; Jian, Y.; Liu, B.; Yuan, Y.; Qin, K.; Nai, S.; Huang, X.; Wang, Y.; Chen, Z.; Chen, X.; Dong, M.; Geng, Y.*; Xu, Z.*; Li, J.* “ O-GlcNAcylation Stabilizes the Authophagy-Initiating Kinase ULK1 by Inhibiting Chaperone-Mediated Autophagy upon HPV Infection” J. Biol. Chem. 298, 102341 (2022). Jin, G.; Sun, D.; Xia, X.; Jiang, Z.; Cheng, B.; Ning, Y.; Wang, Y.; Zhao, Y.*; Chen, X.*; Zhang, J.* “ Bioorthogonal Lanthanide Molecular Probes for Near-Infrared Fluorescence and Mass Spectrometry Imaging” Angew. Chem. Int. Ed. 61, e202208707 (2022). Lv, P.; Du, Y.; He, C.; Peng, L.; Zhou, X.; Wan, Y.; Zeng, M.; Zhou, W.; Zou, P.; Li, C.; Zhang, M.; Dong, S.*; Chen, X.* “ O-GlcNAcylation modulates liquid–liquid phase separation of SynGAP/PSD-95” Nat. Chem. 14, 831-840 (2022). Fan, X.; Song, Q.; Sun, D.; Hao, Y.; Wang, J.; Wang, C.; Chen, X.* “ Cell-type-specific labeling and profiling of glycans in living mice” Nat. Chem. Biol. 18, 625-633 (2022). Zhao, W.; Zhao, S.; Li, Liuju; Huang, X.; Xing, S.; Zhang, Y.; Qiu, G.; Han, Z.; Shang, Y.; Sun, D.; Shan, C.; Wu, R.; Gu, L.; Zhang, S.; Chen, R.; Xiao, J.; Mo, Y.; Wang, J.; Ji, W.; Chen, X.; Ding, B.; Liu, Y,; Mao, H.; Song, B.; Tan, J.; Liu, J.; Li, H.*; Chen, L.* “ Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy” Nat. Biotechnol. 40, 606-617 (2022). Cheng, B.*; Wan, Y.; Tang, Q.; Du, Y.; Chen, X.* “ A Photocaged Azidosugar for Light-Controlled Metabolic Labeling of Cell-surface Sialoglycans” Chin. J. Chem. 40, 806-812 (2022). Liu, J.; Hao, Y.*; Wang, C.; Jin, Y.; Yang, Y.; Gu, J.*; Chen, X.* “ An Optimized Isotopic Photocleavable Tagging Strategy for Site-Specific and Quantitative Profiling of Protein O-GlcNAcylation in Colorectal Cancer Metastasis” ACS Chem. Biol. 17, 513-520 (2022). Tang, Q.; Liu, L.; Guo, Y.; Zhang, X.; Zhang, S.; Jia, Y.; Du, Y.; Cheng, B.; Yang, L.*; Huang, Y.*; Chen, X.* “ Optical Cell Tagging for Spatially Resolved Single-Cell RNA Sequencing” Angew. Chem. Int. Ed. 61, e202113929 (2022). Liu, J.; Hao, Y.; He, Y.; Li, X.; Sun, D.; Zhang, Y.; Yang, P.; Chen, X.* “ Quantitative and Site-Specific Chemoproteomic Profiling of Protein O-GlcNAcylation in the Cell Cycle” ACS Chem. Biol. 16, 1917-1923 (2021). Cheng, B.;Tang, Q.;Zhang, C.; Chen, X.* “ Glycan Labeling and Analysis in Cells and In Vivo” Annu. Rev. Anal. Chem. 14, 363-387 (2021). Liu, J; Shao, X.; Qin, W.; Zhang, Y.; Dang, F.; Yang, Q.; Yu, X.; Li, Y.; Chen, X.*; Wang, C*; Wang, Y.* “ Quantitative chemoproteomics reveals O-GlcNAcylation of cystathionine γ-lyase (CSE) represses trophoblast syncytialization” Cell Chem. Biol. 28, 788-781 (2021). Tang, Q.; Guo, Y.; Meng, L.; Chen, X.* “ Chemical Tagging of Protein Lipoylation” Angew. Chem. Int. Ed. 60, 4028-4033 (2021). Sun, D.; Fan, X.;Shi, Y.; Zhang, H.; Huang, Z.; Cheng, B.; Tang, Q.; Li, W.; Zhu, Y.; Bai, J.; Liu, W.; Li, Y.; Wang, X.; Lei, X.; Chen, X.* “ Click-ExM enables expansion microscopy for all biomolecules” Nat. Methods18, 107-113 (2021). Meng, L.; Guo, Y.; Tang, Q.; Huang, R.; Xie, Y.; Chen, X.* “ Metabolic RNA labeling for probing RNA dynamics in bacteria” Nucleic Acids Res. 48, 12566-12576 (2020). Hong, S.; Chen, T.; Liu, L.; Cao, C.; Lv, F.; Rabinowitz, J.D.; Huang, Y.*; Chen, X.* “ Live-Cell Imaging of NADPH Production from Specific Pathways” CCS Chem. 2, 1642-1648 (2020). Cheng, B.; Chen, X.* “ Chemical Labeling and Analysis of Sialoglycans” Chin. Sci. Bull. 65, 2984-2997 (2020). Qin, K; Zhang, H.; Zhao, Z.; Chen, X.* “ Protein S-Glyco-Modification Through an Elimination-Addition Mechanism” J. Am. Chem. Soc. 142, 9382-9388 (2020). Liu, C.; Shi, Y.; Li, J.; Liu, X.; Xiahou, Z.; Tan, Z.; Chen, X.; Li, J.* “ O-GlcNAcylation of Myosin Phosphatase Targeting Subunit 1 (MYPT1) Dictates Timely Disjunction of Centrosomes” J. Biol. Chem. 295, 7341-7349 (2020). Qin, W.; Xie, Z.; Wang, J.; Ou, G.; Wang, C.*; Chen, X.* “ Chemoproteomic Profiling of O-GlcNAcylation in Caenorhabditis elegans” Biochemistry 59, 3129-3134 (2020). Cheng, B.; Dong, L.; Zhu, Y.; Huang, R.; Sun, Y.; You, Q.; Song, Q.; Paton, J.C.; Paton, A.W.; Chen, X.* “ 9-Azido Analogs of Three Sialic Acid Forms for Metabolic Remodeling of Cell-Surface Sialoglycans” ACS Chem. Biol. 14， 2141-2147 (2019) Hao, Y.; Fan, X.; Shi, Y.; Zhang, C.; Sun, D.; Qin, K.; Qin, W.; Zhou, W.; Chen, X.* “ Next-Generation Unnatural Monosaccharides Reveal that ESRRB O-GlcNAcylation Enhances Pluripotency of Mouse Embryonic Stem Cells” Nat. Comm. 10, 4065 (2019). Qin, W.; Qin, K.; Zhang, Y.; Jia, W.; Chen, Y.; Cheng, B.; Peng, L.; Chen, N.; Liu, Y.; Zhou, W.; Wang, Y.; Chen, X.*; Wang, C.* “ S-Glycosylation-Based Cysteine Profiling Reveals Negative-Feedback Regulation of Glycolysis by Itaconate.” Nat. Chem. Biol. 15, 983-991 (2019). Wang, W.; Lin, L.; Du, Y.; Song, Y.; Peng, X.; Chen, X.*; Yang, J. C.* “ Assessing the Viability of Transplanted Gut Microbiota by Sequential Tagging with D-Amino Acid-Based Metabolic Probes.” Nat. Comm. 10, 1317 (2019). Li, Y.; Liu, W.; Tang, Q.; Fan, X.; Hao, Y.; Gao, L.; Li, Z.; Cheng, B.; Chen, X.* “ Gap-Junction-Dependent Labeling of Nascent Proteins in Multicellular Networks.” ACS Chem. Biol. 14, 182-185 (2019). Gao, L.; Song, Q.; Liang, H.; Zhu, Y.; Wei, T.; Dong, N.; Xiao, J.; Shao, F.; Chen, X.* “ Legionella Effector SetA as A General O-glucosyltransferase for Eukaryotic Proteins.” Nat. Chem. Biol. 15, 213-216 (2019). Huang, R.; Han, M.; Meng, L.; Chen, X.* “ Capture and Identification of RNA-Binding Proteins By Using Click Chemistry-Assisted RNA-Interactome Capture (CARIC) Strategy” J. Vis. Exp. 140, e58580 (2018). Liang, L.; Tang, H; Deng, Z.; Liu, Y,; Chen, X.*; Wang, H.* “ Ag Nanoparticles Inhibit the Growth of the Bryophyte, Physcomitrella Patens.” Ecotoxicol. Environ. Saf. 164, 739-748 (2018). Qin, K.; Zhu, Y; Qin, W.; Gao, J.; Shao, X.; Wang, Y.; Zhou, W.*; Wang, C.*; Chen, X.* “ Quantitiative Profiling of Protein O-GlcNAcylation Sites by an Isotope-Tagged Cleavable Linker” ACS Chem. Biol. 13, 1983-1989 (2018). Bai, Q.; Dong L.; Hao, Y.; Chen, X.*; Shen, Q.* “ Metabolic Glycan Labeling-Assisted Discovery of Cell-Surface Markers for Primary Neural Stem and Progenitor Cells” Chem. Comm. 54, 5486-5489 (2018). Huang, R.; Han, M.; Meng, L; Chen, X.* “ Transcriptome-wide discovery of coding and noncoding RNA-binding proteins” Proc. Natl. Acad. Sci. USA 115, E3879-E3887 (2018). Wang, W.; Chen, X.* “ Antibiotics-Based Fluorescent Probes for Selective Labeling of Gram-Negative and Gram-Positive Bacteria in Living Microbiota” Sci. China Chem. 61, 792-796 (2018). Xu, Y.; Peng, L.; Wang, S.; Wang, A.; Ma, R.; Zhou, Y.; Yang, J.; Sun, D.; Lin, W.; Chen, X.; Zou, P.* “ Hybrid Indicators for Fast and Sensitive Voltage Imaging” Angew. Chem. Int. Ed. 57, 3949-3953 (2018). Sun, Y.; Hong, S.; Xie, R.; Huang, R.; Lei, R.; Cheng, B.; Sun, D.; Du, Y.; Nycholat, C.; Paulson, J.; Chen, X.* “ Mechanistic Investigation and Multiplexing of Liposome-Assisted Metabolic Glycan Labeling” J. Am. Chem. Soc. 140, 3592–3602 (2018). Qin, W.; Qin, K.; Fan, X.; Peng, L.; Hong, W.; Zhu, Y.; Lv, P.; Du, Y.; Huang, R.; Han, M.; Cheng, B.; Liu, Y.; Zhou, W.; Zhou, Y.; Wang, C.*; Chen, X.* “Artificial Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling” Angew. Chem. Int. Ed. 57,1817-1820 (2018). Du, Y.; Xie, R.; Sun, Y.; Fan, X.; Chen, X.* “Liposome-Assisted Metabolic Glycan Labeling with Cell and Tissue Selectivity” Methods Enzymol. 598, 321-353 (2018).