个人简介

Joseph A. Loo, Ph.D., is Professor of Biological Chemistry at the UCLA David Geffen School of Medicine and Professor of Chemistry and Biochemistry. He received his Bachelor of Science degree in Chemistry at Clarkson University (Potsdam, NY). He entered the PhD program at Cornell University, where he developed high-resolution Fourier-transform mass spectrometry (MS) methods for large biomolecule characterization in the laboratory of Professor Fred W. McLafferty; he received his PhD in Chemistry in 1988. He was a postdoctoral fellow and later a Senior Scientist at the Pacific Northwest National Laboratory (Richland, WA) with Dr. Richard Smith, where he worked on advanced MS for protein sequencing. He joined Parke-Davis Pharmaceutical (later became Pfizer) in Ann Arbor, MI in 1992, and he became Group Leader of the Biological Mass Spectrometry and Proteomics Teams. In 2001, he joined the UCLA faculty. He is a member of UCLA/DOE Laboratory for Genomics and Proteomics, and the UCLA Molecular Biology Institute. The Loo group focuses on the development of MS and protein chemistry for the characterization of peptides and proteins and the application of MS for proteomics. In 2000-2002, he served on the Board of Directors for the American Society for Mass Spectrometry. He has been on the Editorial Boards of the journals Bioconjugate Chemistry and Analytical Chemistry. Currently, Dr. Loo is on the Editorial Boards of several analytical chemistry journals, and he is an Associate Editor for the Journal of the American Society for Mass Spectrometry.

研究领域

Biochemistry/Structural and Computational Biology/Systems Biology and Biological Regulation/Proteomics and Bioinformatics/Analytical

Research in the Loo lab is aimed at developing and applying high performance mass spectrometry and proteomics to address important questions in biology and medicine. The important role of proteins and protein assemblies in normal cellular processes and diseases warrants a practical and sensitive method for their study. Although methods such as X-ray crystallography and NMR spectroscopy provide unrivaled high-resolution structural information, significantly advanced mass spectrometry (MS) and ion mobility spectrometry (IMS) with higher sensitivity has capabilities to offer structural biologists layers of insight. Mass measurements deliver information on stoichiometry of binding partners directly, even for multi-ligand hetero-complexes and molecular machines with masses well beyond 1 MDa. With electrospray ionization (ESI), MS can measure proteins and complexes from aqueous solution at near neutral pH, i.e., “native” MS. ESI’s special gift for transforming solution-phase macromolecules into gas-phase ionized counterparts without disrupting covalent bonds and weak noncovalent interactions is key for applying MS to study protein complexes.

近期论文

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Lohnes K, Quebbemann NR, Liu K, Kobzeff F, Loo JA, and Ogorzalek Loo RR. “Combining High-throughput MALDI-TOF Mass Spectrometry and Isoelectric Focusing Gel Electrophoresis for Virtual 2D Gel-based Proteomics.” Methods, in press. Levine J, Kwon E, Paez P, Yan W, Czerwieniec G, Loo JA, Sofroniew MV and Wanner I-B. “Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.” Glia, in press. Cheng S, Wang J, Cai Y, Loo JA, and Chen H. “Enhancing Performance of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry Using Trap and Capillary Columns.” Int J Mass Spectrom, in press. Decker CG, Wang Y, Paluck SJ, Shen L, Loo JA, Levine AJ, Miller LS, and Maynard HD. “Fibroblast Growth Factor 2 Dimer with Superagonist In Vitro Activity Improves Granulation Tissue Formation During Wound Healing Biomaterials.” Biomaterials, in press. Jiang J, Chan H, Cash DD, Miracco EJ, Ogorzalek Loo RR, Cascio D, O’Brien Johnson R, Loo JA, Zhou ZH, and Feigon J. “Structure of Tetrahymena telomerase reveals new subunits, functions, and interactions.” Science 2015; 350: 529 (aab4070). Sohn CH, Yin S, Peng I, Loo JA and Beauchamp JL. “Investigation of the Mechanism of Electron Capture and Electron Transfer Dissociation of Peptides with a Covalently Attached Free Radical Hydrogen Atom Scavenger.” Int J Mass Spectrom 2015; 390: 49–55. Wongkongkathep P, Li H, Zhang X, Ogorzalek Loo RR, Julian RR, and Loo JA. “Enhancing Protein Disulfide Bond Cleavage by UV Excitation and Electron Capture Dissociation for Top-Down Mass Spectrometry.” Int J Mass Spectrom 2015; 390: 137–145. Cai Y, Zheng Q, Liu Y, Helmy R, Loo JA and Chen H. “Integration of Electrochemistry with Ultra Performance Liquid Chromatography/Mass Spectrometry (UPLC/MS).” Eur J Mass Spectrom 2015; 21: 341–351. Zhang J, Malmirchegini GR, Clubb RT, and Loo JA. “Native Top-Down Mass Spectrometry for the Structural Characterization of Human Hemoglobin.” Eur J Mass Spectrom 2015; 21: 221–231.