个人简介

During his graduate and postdoctoral work with Harden McConnell at Stanford University, Wayne Hubbell used spin label technology to first describe the fluidity and fluidity gradient in biological membranes, landmark discoveries of broad impact in cell and membrane biology. In 1970, he joined the faculty of the Department of Chemistry at UC Berkeley where his laboratory designed new surfactants, pioneered the molecular characterization of reconstituted membrane proteins, and developed a series of unique spin label probes to study membrane electrostatics. In 1983, Prof. Hubbell moved his laboratory to UCLA where he became the first Jules Stein Professor of Ophthalmology and Professor of Chemistry and Biochemistry. Soon after arriving, he combined unrelated technical advancements in molecular biology and EPR spectroscopy and pioneered the powerful new technology of site-directed spin labeling (SDSL) for the determination of structure and conformational dynamics in both soluble and membrane proteins. For his development and application of SDSL, Prof. Hubbell has received numerous honors and awards including the Gold Medal of the International EPR/ESR Society, the International Zavoisky Award from the Physical Technical Institute of the Russian Academy of Sciences, the Bruker Prize from the Royal Society of Chemistry-ESR Group, and the Elisabeth Roberts Cole from the Biophysical Society (US). He is a member of the National Academy of Sciences, American Academy of Arts and Sciences, elected to the first class of fellows of the Biophysical Society and recipient of an honorary doctorate from the University of Pécs, Hungary.

研究领域

Biochemistry/Biophysics/Structural and Computational Biology/Proteomics and Bioinformatics

Dr. Hubbell's research is focused on understanding the inter-relationship of protein structure, dynamics and function. Of particular interest are proteins involved in visual signal transduction (rhodopsin, the G-protein transducin and arrestin) and soluble ligand-binding proteins. To explore functional protein dynamics in such complex systems, his laboratory has pioneered the technique of "Site-Directed Spin Labeling" (SDSL) in which a paramagnetic nitroxide side chain (R1) is introduced into the protein as a molecular sensor. The technique exploits the sensitivity of Electron Paramagnetic Resonance (EPR) to motions of R1 in the biologically important nanosecond and microsecond time domains that correspond to backbone fluctuations and conformational switching, respectively. Research in the group involves a parallel development of new EPR and SDSL technology and applications to the systems mentioned above.

近期论文

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chredelseker J, Paz A, Lopez C, Chen J-N, Hubbell W, Abramson J. High-resolution structure and double electron-electron resonance of zebrafish voltage dependent anion channel 2 reveal an oligomeric population. J Biol Chem 289:12566-77 (2014) PMCID4007448 Lerch MT, Yang Z, Brooks EK, Hubbell WL. Mapping protein heterogeneity under pressure with site-directed spin labeling and double electron-electron resonance. Proc Natl Acad Sci USA 111:R1201-10 (2014) PMCID3977274 Chen Q, Zhou Y, Kim M, Hanson SM, Francis DJ, Vishnivetskiy SA, Altenbach C, Klug CS, Hubbell WL, Gurevich VV. Self-association of arrestin family members. Handb Exp Pharmacol 219:205-23 (2014) Lerch MT, McCoy J, Horwitz J, Hubbell WL. Circular dichroism and site-directed spin labeling reveal structural and dynamical features of high-pressure states of myoglobin. Proc Natl Acad Sci USA 110:E4714-22 (2013) PMCID3856799 Lopez CJ, Yang Z, Altenbach C, Hubbell WL. Conformational selection and adaptation to ligand binding in T4 lysozyme cavity mutants. Proc Natl Acad Sci USA 110:E4306-15 (2013) PMCID3832024 Razzaghi S, Brooks EK, Bordignon E, Hubbell WL, Yulikov M, Jeschke G. EPR relaxation-enhancement-based distance measurements on orthogonally spin-labeled T4-lysozyme. Chembiochem 14:1883-90 (2013) PMCID3804414 Hubbell WL, Lopez CJ, Altenbach C, Yang Z. Technological advances in site-directed spin labeling of proteins. Curr Opin Struct Biol 23:725-33 (2013) PMCID3805720 Song X, Seo J, Baarneur F, Vishnivetskiy S, Chen Q, Kim M, Brooks E, Altenbach C, Hong Y, Hanson S, Palazzo M, Chen J, Hubbell W, Gurevich E, Gurevich V. Rapid degeneration of rod photoreceptors expressing self-association-deficient arrestin-1-mutant. Cell Signal 25:2613-24 (2013) PMCID3833262 Toledo-Warshaviak D, Khramtzov V, Cascio D, Altenbach C, Hubbell W. Structure and dynamics of an imidazoline nitroxide side chain with strongly hindered internal motion in proteins. J Mag Reson 232C:53-61 (2013) PMCID3758229